LOCUS       pACYCDuet1_6xHIS        4468 bp ds-DNA     circular     21-APR-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    71..439
                     /locus_tag="6xHis-HRV3C-Pa_IHFa"
                     /label="6xHis-HRV3C-Pa_IHFa"
                     /ApEinfo_label="6xHis-HRV3C-Pa_IHFa"
                     /ApEinfo_fwdcolor="#804040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4452..4468
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    511..527
                     /locus_tag="T7 promoter(1)"
                     /label="T7 promoter(1)"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    608..892
                     /locus_tag="PA_IHFbeta"
                     /label="PA_IHFbeta"
                     /ApEinfo_label="PA_IHFbeta"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG
       61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGatc ctctggaagt
      121 gctgtttcag ggtccgatgg gggctctgac gaaagctgaa attgcggaac gtctgtacga
      181 agagctgggc ctgaacaaac gggaagccaa ggagttggtg gagctgttct tcgaagagat
      241 ccgccaggcg ctggagcaca acgagcaggt caagctgtcc ggtttcggca acttcgacct
      301 gcgcgacaag cgccagcgcc ccggacgcaa cccgaagacc ggtgaagaaa tcccgatcac
      361 ggcccgccgc gttgtcacct ttcgtccagg gcagaaactg aaggccaggg ttgaggcata
      421 tgctggaacc aagtcataaa agcttgcggc cgcataatgc ttaagtcgaa cagaaagtaa
      481 tcgtattgta cacggccgca taatcgaaat taatacgact cactataggg gaattgtgag
      541 cggataacaa ttccccatct tagtatatta gttaagtata agaaggagat atacatatgg
      601 cagatctatg accaagtcgg agttgatcga acggatcgtt acccatcagg ggcaactgtc
      661 cgcgaaggat gtcgagttgg caatcaagac catgctggag caaatgtccc aagccctggc
      721 gactggggac cggatcgaga tccgtggctt cggcagcttt tccttgcact accgcgcccc
      781 gcgcgtcggt cgcaacccca agaccgggga gtcggtacgc ctcgacggca agttcgtgcc
      841 gcacttcaag ccgggcaagg agttgcggga tcgggtcaac gagccggagt gacctaggct
      901 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      961 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     1021 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     1081 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     1141 aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     1201 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1261 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1321 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1381 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     1441 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1501 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     1561 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     1621 tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     1681 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     1741 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     1801 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     1861 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     1921 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     1981 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     2041 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
     2101 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
     2161 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga
     2221 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     2281 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     2341 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     2401 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     2461 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     2521 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg
     2581 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     2641 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     2701 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     2761 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     2821 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     2881 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     2941 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     3001 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     3061 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     3121 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     3181 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     3241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     3361 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     3421 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     3481 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     3541 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     3601 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     3661 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     3721 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     3781 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     3841 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     3901 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     3961 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     4021 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     4081 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     4141 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     4201 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     4261 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     4321 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     4381 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     4441 cattaggaaa ttaatacgac tcactata   
//
