LOCUS       pPSup                   5471 bp ds-DNA     circular     29-JAN-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(1307..1337)
                     /label="lac promoter"
     primer_bind     complement(1259..1275)
                     /label="M13 rev"
     promoter        27..45
                     /label="T7 promoter"
     CDS             complement(2486..3274)
                     /label="SmR"
     primer_bind     71..87
                     /label="KS primer"
     CDS             183..899
                     /label="EGFP"
     protein_bind    1283..1299
                     /label="lac operator"
     rep_origin      3565..4000
                     /label="pSa ori"
     terminator      4181..4433
                     /label="NOS terminator"
     misc_feature    948..1147
                     /label="polyA rbcS"
     misc_feature    147..182
                     /label="BbsI cassette for promoter+UTR+bc"
     protein_bind    1352..1373
                     /label="CAP binding site"
     misc_feature    109..146
                     /label="BsaI cassette for enhancer"
     CDS             complement(4453..5004)
                     /label="BlpR"
     promoter        complement(5046..5225)
                     /label="NOS promoter"
     rep_origin      complement(1727..2315)
                     /label="ori"
     primer_bind     1..17
                     /label="M13 fwd"
     misc_feature    1612..1636
                     /label="RB T-DNA repeat"
     misc_feature    4135..4157
                     /label="LB T-DNA repeat"
     promoter        complement(1220..1238)
                     /label="T3 promoter"
     primer          complement(265..286)
                     /label="TJ1275"
                     /note="sequence: CAAAGGTAGAAGCAGAAACTTACCTTCGCCCTCGCCGGACACGC"
     primer          1314..1333
                     /label="TJ1248"
                     /note="sequence: CGAGCCGGAAGCATAAAGTG"
     primer          complement(1616..1636)
                     /label="TJ1246"
                     /note="sequence: GTTTACCCGCCAATATATCCT"
     primer          4135..4154
                     /label="TJ1245"
                     /note="sequence: TGGCAGGATATATTGTGGTG"
     primer          285..306
                     /label="TJ1274"
                     /note="sequence: GACCAAAATTTGTTGATGTGCAGGTGATGCCACCTACGGCAAGC"
     primer          complement(4271..4293)
                     /label="TJ1247"
                     /note="sequence: GGTTTTTATGATTAGAGTCCCGC"
ORIGIN
        1 GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG
       61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCAC TCCGAGACCA
      121 CAAGGCGCGC CTAGTGGTCT CCAGGAGCTG CCGTCTTCAG TGCCTGCAGG CATAGAAGAC
      181 CCGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
      241 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
      301 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
      361 TCGTGACCAC CTTCACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
      421 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
      481 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
      541 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
      601 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
      661 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
      721 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
      781 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
      841 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCACGGCAT GGACGAGCTG TACAAGTAAA
      901 GCGGCCGCAA ATTGCCCAGA ATTAATTCCC CAACTGCACA GAACAAATTT CCCTTTGCTT
      961 TTGTGTAGAC CTCAAAACTT TATCCCCCAT CTTTGATCTT ATCCCTTGTT TTTCTGCTTT
     1021 TTTCTTCTTT CTTGGGTTTT GATTTCCGGA CTTAACGTTT GTTTTCCGGT TTGCGAGACA
     1081 TATTCTATCG GATTCTCAAC TGTCTGATGA AATAAATATG TAATGTTCTA TAAGTCTTTC
     1141 AATTTGATGG GATGTTCCAC TTCTGTATAG GAGCCAATTG ATTGACAACA TGCATCAATC
     1201 GGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT
     1261 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG
     1321 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT
     1381 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG
     1441 GCCAACGCTC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
     1501 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
     1561 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGAAGGCC TTGACAGGAT
     1621 ATATTGGCGG GTAAACTAAG TCGCTGTATG TGTTTGTTTG AGATCTCATG TGAGCAAAAG
     1681 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     1741 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     1801 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     1861 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     1921 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     1981 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     2041 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     2101 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     2161 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAGAAGAG
     2221 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     2281 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     2341 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     2401 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     2461 TATATGTGTA ACATTGGTCT AGTGATTATT TGCCGACTAC CTTGGTGATC TCGCCTTTCA
     2521 CGTAGTGAAC AAATTCTTCC AACTGATCTG CGCGCGAGGC CAAGCGATCT TCTTGTCCAA
     2581 GATAAGCCTG CCTAGCTTCA AGTATGACGG GCTGATACTG GGCCGGCAGG CGCTCCATTG
     2641 CCCAGTCGGC AGCGACATCC TTCGGCGCGA TTTTGCCGGT TACTGCGCTG TACCAAATGC
     2701 GGGACAACGT AAGCACTACA TTTCGCTCAT CGCCAGCCCA GTCGGGCGGC GAGTTCCATA
     2761 GCGTTAAGGT TTCATTTAGC GCCTCAAATA GATCCTGTTC AGGAACCGGA TCAAAGAGTT
     2821 CCTCCGCCGC TGGACCTACC AAGGCAACGC TATGTTCTCT TGCTTTTGTC AGCAAGATAG
     2881 CCAGATCAAT GTCGATCGTG GCTGGCTCGA AGATACCTGC AAGAATGTCA TTGCGCTGCC
     2941 ATTCTCCAAA TTGCAGTTCG CGCTTAGCTG GATAACGCCA CGGAATGATG TCGTCGTGCA
     3001 CAACAATGGT GACTTCTACA GCGCGGAGAA TCTCGCTCTC TCCAGGGGAA GCCGAAGTTT
     3061 CCAAAAGGTC GTTGATCAAA GCTCGCCGCG TTGTTTCATC AAGCCTTACG GTCACCGTAA
     3121 CCAGCAAATC AATATCACTG TGTGGCTTCA GGCCGCCATC CACTGCGGAG CCGTACAAAT
     3181 GTACGGCCAG CAACGTCGGT TCGAGATGGC GCTCGATGAC GCCAACTACC TCTGATAGTT
     3241 GAGTCGATAC TTCGGCGATC ACCGCTTCCC TCATAACACC CCTTGTATTA CTGTTTATGT
     3301 AAGCAGACAG TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA
     3361 GATTTTGAGA CACAACGTGG CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC
     3421 AGATCACGCA TCTTCCCGAC AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA
     3481 CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG
     3541 ATGATGGGGC GATTCAGGCG ATCCCCATCC AACAGCCCGC CGTCGAGCGG GCTTTTTTAT
     3601 CCCCGGAAGC CTGTGGATAG AGGGTAGTTA TCCACGTGAA ACCGCTAATG CCCCGCAAAG
     3661 CCTTGATTCA CGGGGCTTTC CGGCCCGCTC CAAAAACTAT CCACGTGAAA TCGCTAATCA
     3721 GGGTACGTGA AATCGCTAAT CGGAGTACGT GAAATCGCTA ATAAGGTCAC GTGAAATCGC
     3781 TAATCAAAAA GGCACGTGAG AACGCTAATA GCCCTTTCAG ATCAACAGCT TGCAAACACC
     3841 CCTCGCTCCG GCAAGTAGTT ACAGCAAGTA GTATGTTCAA TTAGCTTTTC AATTATGAAT
     3901 ATATATATCA ATTATTGGTC GCCCTTGGCT TGTGGACAAT GCGCTACGCG CACCGGCTCC
     3961 GCCCGTGGAC AACCGCAAGC GGTTGCCCAC CGTCGAGCGC CAGCGCCTTT GCCCACAACC
     4021 CGGCGGCCGG CCGCAACAGA TCGTTTTATA AATTTTTTTT TTTGAAAAAG AAAAAGCCCG
     4081 AAAGGCGGCA ACCTCTCGGG CTTCTGGATT TCCGATCCCC GGAATTAGAG ATCTTGGCAG
     4141 GATATATTGT GGTGTAACGT TATCAGCTTG CATGCCGGTC GATCTAGTAA CATAGATGAC
     4201 ACCGCGCGCG ATAATTTATC CTAGTTTGCG CGCTATATTT TGTTTTCTAT CGCGTATTAA
     4261 ATGTATAATT GCGGGACTCT AATCATAAAA ACCCATCTCA TAAATAACGT CATGCATTAC
     4321 ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA
     4381 CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCTGCTTGA
     4441 CTCTAGGGGT CATCAGATTT CGGTGACGGG CAGGACCGGA CGGGGCGGCA CCGGCAGGCT
     4501 GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG
     4561 CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG
     4621 CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG
     4681 GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTGGA
     4741 CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGA CCCGCGTAGG CGATGCCGGC
     4801 GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC
     4861 GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT
     4921 GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC
     4981 GGCCGGGCGT CGTTCTGGGC TCATGGTAGA TCCCCTCGAT CGAGTTGAGA GTGAATATGA
     5041 GACTCTAATT GGATACCGAG GGGAATTTAT GGAACGTCAG TGGAGCATTT TTGACAAGAA
     5101 ATATTTGCTA GCTTATAGTG ACCTTAGGCG ACTTTTGAAC GCGCAATAAT GGTTTCTGAC
     5161 GTATGTGCTT AGCTCATTAA ACTCCAGAAA CCCGCGGCTC AGTGGCTCCT TCAACGTTGC
     5221 GGTTCTGTCA GTTCCAAACG TAAAACGGCT TGTCCCGCGT CATCGGCGGG GGTCATAACG
     5281 TGACTCCCTT AATTCTCCGC TCATGATCGA TAACATTAAC GCTTACAATT TCCATTCGCC
     5341 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA
     5401 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA
     5461 GTCACGACGT T
//