LOCUS       pPSdown2                5483 bp ds-DNA     circular     28-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    121..156
                     /label="BbsI cassette for promoter+UTR+bc"
     primer_bind     71..87
                     /label="KS primer"
     promoter        complement(1194..1212)
                     /label="T3 promoter"
     protein_bind    1326..1347
                     /label="CAP binding site"
     misc_feature    1624..1648
                     /label="RB T-DNA repeat"
     primer_bind     complement(1233..1249)
                     /label="M13 rev"
     misc_feature    4147..4169
                     /label="LB T-DNA repeat"
     promoter        complement(5058..5237)
                     /label="NOS promoter"
     rep_origin      3577..4012
                     /label="pSa ori"
     misc_feature    922..1121
                     /label="polyA rbcS"
     terminator      4193..4445
                     /label="NOS terminator"
     CDS             complement(4465..5016)
                     /label="BlpR"
     CDS             complement(2498..3286)
                     /label="SmR"
     promoter        27..45
                     /label="T7 promoter"
     CDS             157..873
                     /label="EGFP"
     primer_bind     1..17
                     /label="M13 fwd"
     protein_bind    1257..1273
                     /label="lac operator"
     promoter        complement(1281..1311)
                     /label="lac promoter"
     rep_origin      complement(1739..2327)
                     /label="ori"
     misc_feature    1566..1603
                     /label="BsaI cassette for enhancer"
     primer          1288..1307
                     /label="TJ1248"
                     /note="sequence: CGAGCCGGAAGCATAAAGTG"
ORIGIN
        1 GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG
       61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCGC GGCCGCAGGA
      121 GCTGCCGTCT TCAGTGCCTG CAGGCATAGA AGACCCGTGA GCAAGGGCGA GGAGCTGTTC
      181 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC
      241 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC
      301 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCAC CTACGGCGTG
      361 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG
      421 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC
      481 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC
      541 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC
      601 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC
      661 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC
      721 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC
      781 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG
      841 ATCACTCACG GCATGGACGA GCTGTACAAG TAAAGCGGCC GCAAATTGCC CAGAATTAAT
      901 TCCCCAACTG CACAGAACAA ATTTCCCTTT GCTTTTGTGT AGACCTCAAA ACTTTATCCC
      961 CCATCTTTGA TCTTATCCCT TGTTTTTCTG CTTTTTTCTT CTTTCTTGGG TTTTGATTTC
     1021 CGGACTTAAC GTTTGTTTTC CGGTTTGCGA GACATATTCT ATCGGATTCT CAACTGTCTG
     1081 ATGAAATAAA TATGTAATGT TCTATAAGTC TTTCAATTTG ATGGGATGTT CCACTTCTGT
     1141 ATAGGAGCCA ATTGATTGAC AACATGCATC AATCGGAGCT CCAGCTTTTG TTCCCTTTAG
     1201 TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
     1261 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
     1321 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     1381 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCTCGGGGAG AGGCGGTTTG
     1441 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     1501 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     1561 AACGCACTCC GAGACCACAA GGCGCGCCTA GTGGTCTCCA GGAAGGAAAG AACATGAAGG
     1621 CCTTGACAGG ATATATTGGC GGGTAAACTA AGTCGCTGTA TGTGTTTGTT TGAGATCTCA
     1681 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
     1741 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
     1801 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
     1861 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
     1921 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     1981 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     2041 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     2101 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     2161 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     2221 TCGGAAGAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     2281 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     2341 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     2401 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT
     2461 CAATCTAAAG TATATATGTG TAACATTGGT CTAGTGATTA TTTGCCGACT ACCTTGGTGA
     2521 TCTCGCCTTT CACGTAGTGA ACAAATTCTT CCAACTGATC TGCGCGCGAG GCCAAGCGAT
     2581 CTTCTTGTCC AAGATAAGCC TGCCTAGCTT CAAGTATGAC GGGCTGATAC TGGGCCGGCA
     2641 GGCGCTCCAT TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG GTTACTGCGC
     2701 TGTACCAAAT GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC CAGTCGGGCG
     2761 GCGAGTTCCA TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG
     2821 GATCAAAGAG TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT CTTGCTTTTG
     2881 TCAGCAAGAT AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT
     2941 CATTGCGCTG CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA
     3001 TGTCGTCGTG CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC TCTCCAGGGG
     3061 AAGCCGAAGT TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA
     3121 CGGTCACCGT AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA TCCACTGCGG
     3181 AGCCGTACAA ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA
     3241 CCTCTGATAG TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATAACA CCCCTTGTAT
     3301 TACTGTTTAT GTAAGCAGAC AGTTTTATTG TTCATGATGA TATATTTTTA TCTTGTGCAA
     3361 TGTAACATCA GAGATTTTGA GACACAACGT GGCTTTGTTG AATAAATCGA ACTTTTGCTG
     3421 AGTTGAAGGA TCAGATCACG CATCTTCCCG ACAACGCAGA CCGTTCCGTG GCAAAGCAAA
     3481 AGTTCAAAAT CACCAACTGG TCCACCTACA ACAAAGCTCT CATCAACCGT GGCTCCCTCA
     3541 CTTTCTGGCT GGATGATGGG GCGATTCAGG CGATCCCCAT CCAACAGCCC GCCGTCGAGC
     3601 GGGCTTTTTT ATCCCCGGAA GCCTGTGGAT AGAGGGTAGT TATCCACGTG AAACCGCTAA
     3661 TGCCCCGCAA AGCCTTGATT CACGGGGCTT TCCGGCCCGC TCCAAAAACT ATCCACGTGA
     3721 AATCGCTAAT CAGGGTACGT GAAATCGCTA ATCGGAGTAC GTGAAATCGC TAATAAGGTC
     3781 ACGTGAAATC GCTAATCAAA AAGGCACGTG AGAACGCTAA TAGCCCTTTC AGATCAACAG
     3841 CTTGCAAACA CCCCTCGCTC CGGCAAGTAG TTACAGCAAG TAGTATGTTC AATTAGCTTT
     3901 TCAATTATGA ATATATATAT CAATTATTGG TCGCCCTTGG CTTGTGGACA ATGCGCTACG
     3961 CGCACCGGCT CCGCCCGTGG ACAACCGCAA GCGGTTGCCC ACCGTCGAGC GCCAGCGCCT
     4021 TTGCCCACAA CCCGGCGGCC GGCCGCAACA GATCGTTTTA TAAATTTTTT TTTTTGAAAA
     4081 AGAAAAAGCC CGAAAGGCGG CAACCTCTCG GGCTTCTGGA TTTCCGATCC CCGGAATTAG
     4141 AGATCTTGGC AGGATATATT GTGGTGTAAC GTTATCAGCT TGCATGCCGG TCGATCTAGT
     4201 AACATAGATG ACACCGCGCG CGATAATTTA TCCTAGTTTG CGCGCTATAT TTTGTTTTCT
     4261 ATCGCGTATT AAATGTATAA TTGCGGGACT CTAATCATAA AAACCCATCT CATAAATAAC
     4321 GTCATGCATT ACATGTTAAT TATTACATGC TTAACGTAAT TCAACAGAAA TTATATGATA
     4381 ATCATCGCAA GACCGGCAAC AGGATTCAAT CTTAAGAAAC TTTATTGCCA AATGTTTGAA
     4441 CGATCTGCTT GACTCTAGGG GTCATCAGAT TTCGGTGACG GGCAGGACCG GACGGGGCGG
     4501 CACCGGCAGG CTGAAGTCCA GCTGCCAGAA ACCCACGTCA TGCCAGTTCC CGTGCTTGAA
     4561 GCCGGCCGCC CGCAGCATGC CGCGGGGGGC ATATCCGAGC GCCTCGTGCA TGCGCACGCT
     4621 CGGGTCGTTG GGCAGCCCGA TGACAGCGAC CACGCTCTTG AAGCCCTGTG CCTCCAGGGA
     4681 CTTCAGCAGG TGGGTGTAGA GCGTGGAGCC CAGTCCCGTC CGCTGGTGGC GGGGGGAGAC
     4741 GTACACGGTG GACTCGGCCG TCCAGTCGTA GGCGTTGCGT GCCTTCCAGG GACCCGCGTA
     4801 GGCGATGCCG GCGACCTCGC CGTCCACCTC GGCGACGAGC CAGGGATAGC GCTCCCGCAG
     4861 ACGGACGAGG TCGTCCGTCC ACTCCTGCGG TTCCTGCGGC TCGGTACGGA AGTTGACCGT
     4921 GCTTGTCTCG ATGTAGTGGT TGACGATGGT GCAGACCGCC GGCATGTCCG CCTCGGTGGC
     4981 ACGGCGGATG TCGGCCGGGC GTCGTTCTGG GCTCATGGTA GATCCCCTCG ATCGAGTTGA
     5041 GAGTGAATAT GAGACTCTAA TTGGATACCG AGGGGAATTT ATGGAACGTC AGTGGAGCAT
     5101 TTTTGACAAG AAATATTTGC TAGCTTATAG TGACCTTAGG CGACTTTTGA ACGCGCAATA
     5161 ATGGTTTCTG ACGTATGTGC TTAGCTCATT AAACTCCAGA AACCCGCGGC TCAGTGGCTC
     5221 CTTCAACGTT GCGGTTCTGT CAGTTCCAAA CGTAAAACGG CTTGTCCCGC GTCATCGGCG
     5281 GGGGTCATAA CGTGACTCCC TTAATTCTCC GCTCATGATC GATAACATTA ACGCTTACAA
     5341 TTTCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC
     5401 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC
     5461 AGGGTTTTCC CAGTCACGAC GTT
//