LOCUS       Exported               15673 bp ds-DNA     circular SYN 22-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 22, 2020 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
COMMENT     Comments Assemble of: Fragment  : pTCO2 : NheI at 5350 to NotI at 
            3404.Fragment  : pSUP-spTDG.0 : NotI at 150 to NheI at 
            2094.Assembled on 30.01.2014 with corrected mTDG cDNAThis plasmid 
            was designed to complement TDG KO alleles. It contains the cDNA of 
            mouse TDG with an chimeric intron sequence that can be spliced to 
            produce TDGa and TDGb isoforms. Expression is controlled by about 6 
            kb of mouse TDG promoter sequence and about 3 kb of mouse TDG 
            terminator sequences. It also can be used to produce conditional KO 
            alleles since it contains two Cre/lox sites.The plasmid furthermore 
            carries a bacterial ampicilin resistance (pBlueScript backbone) and 
            a puromycin resistance cassette controlled by SV40 transcription 
            regulators.
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1412..1445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     5'UTR           join(1837..1899,2572..2620)
                     /label=5'UTR Tdg transcript variant 1
                     /note="This feature has 2 segments:
                        1: 1837 .. 1899 / #ffcc00
                        2: 2572 .. 2620 / #ffcc00"
     5'UTR           1837..1975
                     /label=5'UTR Tdg transcript variant 2
                     /note="color: #ffcc00"
     misc_RNA        1837
                     /label=Transcription start mTDGb
                     /note="color: #00ff00; direction: RIGHT"
     misc_RNA        1899
                     /label=splice donor TDGb
                     /note="color: #00ff00; direction: RIGHT"
     intron          1900..2571
                     /label=chimeric Tdg|| beta-globin intron --> TDGb
                     /note="intron from rabbit I²-globin gene"
                     /note="color: #------"
     regulatory      1970..1979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #ff00ff; direction: RIGHT"
     CDS             join(1976..1998,2572..3812)
                     /codon_start=1
                     /label=TDGa
                     /note="This feature has 2 segments:
                        1: 1976 .. 1998 / #ff0000
                        2: 2572 .. 3812 / #ff0000 / TDGa"
                     /translation="MDAEAARSYSLEQVQALYSFPFQQMMAEVPNMAVTTGQQVPAVAP
                     NMATVTEQQVPADAPVQEPAPEAPKRRKRKPRAAEPQEPVEPKKPATSKKSGKSTKSKE
                     KQEKITDAFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHY
                     PGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREG
                     GRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMP
                     SSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNTDVQEVQYTFDLQLAQEDAKKMAV
                     KEEKYDPGYEAAYGGAYGENPCNGEPCGIASNGLTAHSAEPRGEATPGDVPNGQWMAQS
                     FAEQIPSFNNCGTREQEEESHA"
     misc_RNA        1976..1978
                     /label=TDGa start codon
                     /note="color: #00ff00; direction: RIGHT"
     misc_RNA        1998
                     /label=splice donor TDGa (chimeric)
                     /note="color: #00ff00; direction: RIGHT"
     intron          1999..2571
                     /label=beta-globin intron --> TDGa
                     /note="intron from rabbit beta-globin gene"
                     /note="color: #------"
     misc_RNA        complement(2572)
                     /label=splice acceptor (chimeric)
                     /note="color: #00ff00; direction: LEFT"
     CDS             2621..3812
                     /codon_start=1
                     /label=TDGb
                     /note="color: #ff0000"
                     /translation="MMAEVPNMAVTTGQQVPAVAPNMATVTEQQVPADAPVQEPAPEAP
                     KRRKRKPRAAEPQEPVEPKKPATSKKSGKSTKSKEKQEKITDAFKVKRKVDRFNGVSEA
                     ELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHM
                     DDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIY
                     EIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFPRAQDKVHYYIKLKD
                     LRDQLKGIERNTDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCNG
                     EPCGIASNGLTAHSAEPRGEATPGDVPNGQWMAQSFAEQIPSFNNCGTREQEEESHA"
     misc_RNA        2621..2623
                     /label=TDGb start codon
                     /note="color: #00ff00; direction: RIGHT"
     protein_bind    3837..3870
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     3'UTR           3870..5411
                     /label=Tdg 3'UTR
                     /note="color: #ff9900"
     terminator      5412..6876
                     /label=Tdg terminator region
                     /note="This feature has 2 segments:
                        1: 5412 .. 5417 / #00ffff / poly(A) signal
                        2: 5418 .. 6876 / #ffcc99 / terminator"
     promoter        complement(6900..6918)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #cc0000; direction: LEFT"
     polyA_signal    6944..7151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(7195..7213)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ff6c6c; direction: LEFT"
     primer_bind     complement(7234..7250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a4a400; direction: LEFT"
     protein_bind    7258..7274
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-I²-D-thiogalactopyranoside (IPTG)."
                     /note="color: #ffff6f"
     promoter        complement(7282..7312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7282 .. 7288 / #ffffff / -10
                        2: 7289 .. 7306 / #ffffff
                        3: 7307 .. 7312 / #ffffff / -35"
     protein_bind    7327..7348
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #ff8000"
     rep_origin      complement(7636..8224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8395..9255)
                     /codon_start=1
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8395 .. 9186 / #ccffcc
                        2: 9187 .. 9255 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9256..9360)
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      9387..9842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     9983..9999
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #faffdd; direction: RIGHT"
     promoter        10006..10024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffeaea; direction: RIGHT"
     polyA_signal    10055..10176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #99ccff"
     CDS             complement(10398..10997)
                     /codon_start=1
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #0000ff"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(11007..11336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #99ccff; direction: LEFT"
     rep_origin      11021..11156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #c0c0c0"
     rep_origin      11021..11156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #c0c0c0"
     promoter        join(11348..15673,1..1835)
                     /label=Tdg promoter region
                     /note="color: #ff6600; direction: RIGHT"
ORIGIN
        1 taacttctgc atacccaaac ctcttctatg atctaggggt tatgaaggag ccattgccca
       61 agattctcag acacaggaga ctacctggtc tgccaggtac gtctaaggac tgatactgta
      121 aaaccctttg aaggaaccac accctcaaat ctcagataac aatcgctgac aagaggctac
      181 ctcaaagcct aggtgccatc actctgggat tcatggagag ggctagacgc cattagacaa
      241 ctctctggcc aaacacccac agcagacatt gaaaagaaat taaatgaaaa ctcttggggc
      301 tggagagatg gctcagcggt taagaacact gactgctctt tcaaaggtcc tgagttcaaa
      361 tcccagcaac cacatagtgg ctcacaacca tctgtaatga gatctgactg ctctcttctg
      421 gagtgtctga agacagctac agtgtactca cataaataaa taaataaata aataaatcta
      481 aaaaaaaaaa acaaacaaac tcttagaacc tccgaaagtc aagtatggtg atacatgccc
      541 tgtaatccca gtcagtatgc aagagagtga acaggagact tgcctcaggt tgaggccagc
      601 ctggactaca gctgcaaagc tctaagccaa gtcaggggta cacagttttg taattatctc
      661 aaaaaccaaa agaacctccg agaaatcgag gtgagattgc tgtctaaggt tctaaagcag
      721 agatgcatct agcatctagg cgagtgtttg tccatctcca acgtagacaa ctgtttatgg
      781 aaatctttgt aatctaccaa gagcttcccc ttacagatta gtaaactgag gcccaaacat
      841 tggtggcttc caatcttagg ttttccagtt ccaatcagcc ctactcctca tcacaggaat
      901 tgacaagaaa gacaattgaa aagaaagaat gttgttttgt gtaaggcaca atatttatca
      961 ttattataat tatcatttta gtttttcacg acggagtttc tctgtgtagt attgcctgtc
     1021 cggaaactcg ctctataaac taggccagcc tcgaacttag ttctgcctgc cacagtcttt
     1081 gcaaatgctg ggtcgcccag ccaagacaca tagttgcggg ggagggggag ttgttttcat
     1141 tttaatttaa ttaactttac gtcccgttca cagccccacc cccacccagt cccacgctta
     1201 caaatcctgc ccccttctct tctcagagaa gaggaagccc cacttgggta agactctggt
     1261 acatctaatc tcagcaagtg taggcacatc ctttcccact gaggaaacac gcagtcctct
     1321 tcccccagtc cgcgctgggt ccactggcaa ggaacagaga cctagacagc ccctgttcca
     1381 cttgtagggg actcacacgg acaccaagct gataacttcg tatagcatac attatacgaa
     1441 gttataagct tcacatttgc tacaaacgtg tagaggggtc taggtccagc tcctgaaagc
     1501 tccctggttg gtagctcagg ctctgtgagc cccaagtcag gtagctttta atccgcacaa
     1561 aatttggggt gaaaagacat gaatttgcca ccacgcataa tacatcctaa aggccatcac
     1621 ccttaaccca caccttcaag gaaacttaga gcccacaaaa gctgacgtca gccgggcgct
     1681 ccccatcctc tccgagatcc acgtttcccg aaaggctaag cggcggtcgg ggcgccccga
     1741 aaaggacacg ccattggcct ggccagcatc acgtgatggc gaggccgccg gcctcctcgg
     1801 agggggacgg aagtgcgctg gggactcgcc gcggtgatgt agcagcgtgg gaggggccga
     1861 ggccagcgcc gtccatccag cggccgcccc gatcctgtgg tgagtccgcg tgctgagcgg
     1921 cagagcgggc gcccgtgggc tcggcaggag tggccgtacc ctcggcggcg ccgccatgga
     1981 cgcagaggcc gcgcgcaggt gagtttgggg acccttgatt gttctttctt tttcgctatt
     2041 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
     2101 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
     2161 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
     2221 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
     2281 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag
     2341 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct
     2401 ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct
     2461 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc
     2521 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca gctattctct
     2581 ggagcaagtt caagctttgt attcatttcc atttcaacaa atgatggcag aagttcctaa
     2641 catggcagtc acgactggac agcaggtgcc agcagtagct cctaacatgg caaccgtgac
     2701 agaacagcag gtgcccgcag acgctcctgt ccaggaacct gcaccagaag ctccaaagag
     2761 aaggaaaagg aaacccagag cagcagagcc ccaggaacca gtggagccca aaaaacctgc
     2821 tacgtcgaag aaatccggca agtctacaaa atcaaaggaa aagcaggaga aaatcacaga
     2881 cgcgtttaaa gtgaaaagga aagtggaccg cttcaacggc gtctctgaag ctgagcttct
     2941 gaccaagact cttcctgaca ttttgacctt caatctggat attgtgatca ttggcattaa
     3001 cccgggatta atggctgctt acaaaggaca tcactaccct gggcctggaa atcacttctg
     3061 gaagtgtctg ttcatgtcgg ggctgagtga ggtgcagctg aatcacatgg atgaccacac
     3121 cttacccggc aagtacggca tcggattcac caacatggtg gaacggacga cgccgggcag
     3181 caaggatctg tctagtaaag agttccggga aggagggcgc atcctggtgc agaaactgca
     3241 gaaatatcag ccacgaatag cggtgtttaa tggaaaatgt atttatgaaa ttttcagtaa
     3301 agaagttttt ggagtaaagg ttaagaactt ggaatttggg cttcaacccc acaagatccc
     3361 agacacagaa actctgtgct acgtcatgcc gtcgtccagc gccagatgtg ctcagtttcc
     3421 ccgggcccag gacaaagttc attactacat taagctgaag gacttgagag accagctgaa
     3481 aggcattgaa cgcaacaccg acgttcagga agtgcagtat acatttgacc tgcagcttgc
     3541 gcaagaggac gcaaagaaga tggctgttaa ggaagaaaag tatgatccag gctatgaggc
     3601 agcttacggc ggcgcctatg gggaaaaccc atgtaatggg gaaccttgtg gcattgcttc
     3661 aaatgggcta acagcccaca gtgcggagcc gagaggagaa gcgacccccg gcgatgttcc
     3721 aaatgggcag tggatggcac agtcgtttgc agagcagatc ccttctttta ataattgtgg
     3781 gacccgagag caggaagaag agagccacgc ttagtgaaga tctaagctag ctcgagataa
     3841 cttcgtatag catacattat acgaagttat atcgaggaag ggtgcctctc agctcgggct
     3901 cagggctgca gttctaatgc agacctcagg acggagagcc tcagtgtgct aactgagggg
     3961 ctttagtgtt aatggtcctt tacgctagtg gtgtaattat atatggagca gttatatggt
     4021 agagtcaact gtatgaatct gtgtagtttg gaaaggaaaa atagggtgtt ttgtacttga
     4081 gttttgtatt ttaactaccc atcattccag ctttttatat actatatttc atttatgaag
     4141 aaactggttt tctctgagga gtcacttcag tctgtaattt taaaaaagca acagtcggaa
     4201 tatttatagt tgattcttca ctgtgcatac ctatatatgc ccttatccct ttaaatgcct
     4261 aagtagggca tgctactaag aatcaagctg gtgaaaggca gatgtgccta tctctgttgg
     4321 aagccgacta accctccgcc ctccgtggac tcgaagctgg aggtggactc agaaccctct
     4381 gggttgcagt cacacgcaga cccccttact ctcccagtag tgtgtgtggc agttgcttgg
     4441 tagtagaaac ctggtggggc ctgagccctg ccgttggcct ctgcctcttg tggttcctga
     4501 gaaggcagta gaacctggac aattgccacc cctctgtttt actgaatccc ccgtcagtgt
     4561 tcatcactgt tgtgatttta tcccgacatg gaaagcgcta agaaatgaac tttccagtta
     4621 gccacgcgct gctgcaggtg tttgataacc ctgtttaaac tggtgctgtg tacatgggat
     4681 gtactgatac agaacttctc ttctagggaa agctaatcat ttgtcaatga tttaaaaatt
     4741 attgtcacta ctgttgatta ctgtggacta agtgagcctc gtgaaagagc tgattggaat
     4801 ttgtaccttt gtggctgctc atgcattttc catggtgcta cagacagcct gcccgggctg
     4861 gtggctagga aacgtgcgtg ttcagcgatg cctagcgctc tcactcctga gcactctgac
     4921 ttggtgccca cccctggcgc gtgctggttt ggcagtatga aatgcccacg agggttttgt
     4981 atctcacaaa ggttatgtag cctgttctgt gaataatgag ggaagcagtt tcttggtgga
     5041 aattttaaca tgcctttagc tgtggcagtg atgggctttc tttttccttc tgtctgggtc
     5101 aggttgtgca caagtcatgt tatttggaag gtgcactgca ctgctgttta catgggcatt
     5161 ttatgcgggt gctttgaagt gccttgcatc agggattaga atcaattcaa ttctttttca
     5221 tgggactatg caaagcatgt agtattgcct tggtatgtaa ctattgtagt tttaccatgg
     5281 agaaatacct ttaaattata actctggaga gcagccatga ggaccctctg tacttcctgg
     5341 tgcttttcct taacatggtg ggtgagtagc gagtgcgggg caccctgtac atggcagggt
     5401 ggggcgttct cattaaataa tactgagatt ttaaacatgc gcagtctgag cgtttttaca
     5461 ataaacatta agaatggcta tgcttagtat acaggttttc ctccagcagg atttaatggt
     5521 attcctcccc actggaaacc tgaggatggc ttactttctc ttacatgtca catgacaaaa
     5581 atcacaaaag cttgaatagt ttatttttac agattctaca tttttttaat ttataatttt
     5641 gtaaaagaca agactagcaa cctgttttta agaaagaaaa ccctgcagcc agatgtggtg
     5701 gcccacgcct tcagttccag tacttaggag gcaaagacat gaggatcttt gtgagtttga
     5761 agttgtgagt tccaatttat ccggggatat agagaaagac cctgtttgtt tgtttgtttg
     5821 tttttgaggc agcgtttctc tccgtagcct tggctgtcct gaaactcact gtatagacca
     5881 ggctggcctc gaactcacag attaaactct ttctgcctcc tgagtgctga gattaaaggt
     5941 atgtgctgcc actacccggc taacttgttt ctattatttt gaaaacatgt atggatattt
     6001 tgccttccat atgtgcatca catgtgtacc cagtgcctgt gaaggccaga agagaatgtc
     6061 agagcccctg gcactgccat gtgggttctg agaatcaaag ctgggtcctc tggaagagca
     6121 gccaacactc tctccagcca gggagatccc atcttagaag taaaacaaaa gaactgctcc
     6181 caaaatatct aactgagaaa atagaatttt gaaaattttc agatatagtt gtcattcagg
     6241 ttgagacttg gggtacatag cgagaccctg tctcaaaaca ccaaaagaaa agaatgagta
     6301 ggggctgctc atggcaatcg tttgaacttc aagaagttaa gtctatctgt gtgggccttg
     6361 gttcaacttg tacacagtag tatttgtctc ttacaccata ggtcatatat atcagaagag
     6421 ttgctcttaa agaacagtgt tagaagatac agagggcccg ttccattttt atccagggtg
     6481 tattttaact gttggagtgt ttcatctatt gtgatgcagt ttaaagatcc ctgcagggtc
     6541 aggaacaaac cagctttccc ataagtcatt gtgaacacta ggaaagtccc agggtttatg
     6601 tcttccattc cagtggagta gtttagcttc ctgcaggaag tgctccgaat atcttgcatc
     6661 agggttccag ccttcggtgt gtgtggaggg aaataaacaa caggcttgtt agtgacgacg
     6721 attttatatg gaaccttgga tacaagctat ggtagaagtg tctggttagc aggagtcacg
     6781 cctcacataa gcccccttgt tgataaccat acattacata agcctaaggt tatgatagtg
     6841 aagagatctt aatactgcca ttcctcggag tgttctacag tacatctaga gggccctatt
     6901 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     6961 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7021 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7081 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7141 catgctgggg atgcggtggg ctctatgtcg ccgagctggg cccagctttt gttcccttta
     7201 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7261 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7381 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7441 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7501 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7561 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7621 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7801 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7921 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7981 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8041 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8101 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8161 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8221 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8281 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8341 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8401 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8461 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8521 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8581 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8641 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8701 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8761 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8821 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8881 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8941 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9001 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9061 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9121 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9181 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9241 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9301 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9361 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     9421 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9481 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9541 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9601 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9661 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9721 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9781 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9841 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     9901 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     9961 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
    10021 tagggcgaat tgggtacccg gtatacagac atgataagat acattgatga gtttggacaa
    10081 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
    10141 ttatttgtaa ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat
    10201 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
    10261 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
    10321 gtcggtcatt tcgaactcga cgatgcatgg ggtcgtgcgc tcctttcggt cgggcgctgc
    10381 gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
    10441 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
    10501 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
    10561 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
    10621 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
    10681 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
    10741 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
    10801 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
    10861 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac
    10921 gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg
    10981 ggcttgtact cggtcatggt aagctttttg caaaagccta ggcctccaaa aaagcctcct
    11041 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
    11101 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
    11161 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
    11221 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
    11281 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
    11341 cacagggtcg agatcgattc tcctggttcc tgcggttctt tgaaacttta cacagagcca
    11401 cacccaggta cagtaccttt gggtcatggt tttactggaa aagagacctc caatcacctt
    11461 acttaatctg agagtgtttt tgattgtggg ttcatgcttt gattgtgggt tcatttttgt
    11521 taagtaaact gcaagaattt tgaagcatta aggtagcatt gtgtaacagg aatcgtgata
    11581 actttttttt ttaactctgg gcacaaaatt gctcaggatt gggggtggag ggcatttgtc
    11641 attaaacctg actacacaat tttgctcccc cagccccaca tgatggaagc agagatagct
    11701 ctgtaaattc ttttcttaca cacacacagg cacacacaca tgcttttttt taacttcatg
    11761 ataataaaaa aaattagttt taactaaatg gtattaagtt tgacatataa taatctcaca
    11821 gaaagtatac atctggaaga ctatacccac aactaaggca atcaacatta tgcccaagtt
    11881 ttctgtgact gttcatgtgt gggcacatat gcgatttaga ggtcaaagga caacttcagg
    11941 agcattgaat ctcaggcact gtcctttcct ctctttttcc ttttattcct cttccaaact
    12001 gggtgtctca ttgacctgga gctcccaaaa taggataggt tagctgccca gtgaacccaa
    12061 gagatcggct tgtctctgct tcactagagc ttagcccaat tggcgacttc caggtacctg
    12121 tctcagaact aaacatgtaa attaaacatg gtggtttaaa taggtatgag aaatggctca
    12181 acttttaaga gtgggtaggg cttttgcaga ggactagaag gacactggca acactcagga
    12241 aactcacagc catttctagc tccaaacttc tggatcccat gagcacctgt gctcacatgc
    12301 ctggacccac acatcccctc atgcatgtaa ttcaaaatta aaaaaaaaaa ttcaaaaagg
    12361 ctgagcatgg tggcacacac ctttaattcc agttctaggg tagcagaggc agggagatct
    12421 cagtaagttg gacgacagtc tggtctacat ataataaaat tcctgaacag ccaggagtac
    12481 ataaagagac tgtggtggtg gtttgaatag gcatggcccc cacagactca agggtttgaa
    12541 tgcttgaccc atggagagtg gcatgattag aaggtgtggc ctattggagt atatgtggcc
    12601 ttactggagg aggtgtctca ctgggctttg aggtcttaga tgttccagcc aggctgtgtc
    12661 tcactccctt cctgctgcct gccaatcaga catagaactc tgagctacct ctccagcacc
    12721 atgcataact cctgtgtgtc ccagtgcttt ctgccatgat gacagactaa acctctgaaa
    12781 ctataagcca gccccaatgt tttcttataa gagttgcttc ggtcacagtg tctcttcaca
    12841 gctatagaaa ccctaagaca gtaagtaaat aagaataaga tgcagagtaa ctgagaagtt
    12901 atttaatgtc agcctctgac ttccacactt atctacacag gtacatgtac acccaaacag
    12961 acacatacat acaaaataca aattaaaatg catcataata gttcaaactg gacttgagct
    13021 tgcagtgatc ctcctgcctc atcctctcaa ctgctgggct tataagaata taccaccatc
    13081 cactgctctg gctcatagac ttctgtatac ttgtatcctt tggtggagta tctgttcaaa
    13141 tctcttgccc attttttaag atctctttaa tgtcatgcat ttgaatgttt tgcctacatg
    13201 tatgtaccat gtgcatgcct gtgcactcag aggtcggaag agggtgttga acactctgga
    13261 actggagtta caggtggtgt gaaccaccat gtggatgctg ggaactgaac tccagtcctt
    13321 tgcaagaaaa gcatgtgtgt taaacacact ctccagctca atcgacctgt ttggtttttt
    13381 tttttaaact gtaaactgtt ctttattttg gatacaagcc ttttatcaga tacactgcaa
    13441 atgtctcttt ccaatctatg ctttatcttt tccttagtag tataacgaga cacttaaaaa
    13501 tttaaataaa ttccaaattt attacccctc cccacccttt tatgagacaa ggtctctcta
    13561 tcttagttag ggtttgtatt gttctgaaga gacactaaat gttttcttat aagggaaaac
    13621 atttaactgg gaatggccta gtttcagagg ttcatcagag tcgggaggta gcttcttctt
    13681 cttgcatgct gccagagaag aagctgagag ttctacacct tgatccacag gcaccaagag
    13741 cctgttatac cacactgagc atagcttgag catggaagac ctcaaagcct accccacagt
    13801 aatacgcttt ctccaacaag gccacaccta ctactccacc aaggtcatac ttcctaatag
    13861 tgccactcct tatgagccaa acattcaaat acatggtccg tctctatagg gatcatgctt
    13921 attcaaacca cctcagtcta tgtggtccag gctggcctca gaccctagga acaaacagct
    13981 gaggtgcata tcttacatac cagagcagac ctggcctccg ggacctcctg catccctcag
    14041 gccctatctc gaatccctgc cctgaacttt ccagcccagg ggctgggcgg cccttcccac
    14101 agaggctctt tgctacataa cccagacatt ttggtttctt ctcttcttct tcttcttcct
    14161 cctccttttc cttctctcat gcgaacacac tttctctctt tctcttcccc cctcctctct
    14221 ccttccccat ggcgacttcc cctgcctcct tccttgggag cagtaaactt accaggagag
    14281 cagcttccca ataaaccaga agttatatgc attttaatct ggtttgaatt ggctcagttc
    14341 accagtggag aaaaaaactt atcagtgtga ctaaaagtgt gtgccaccat agcccagccc
    14401 ttttggaaaa taaaagtttt gaattacata catatgggga tgtgtgggtc catgtgtgtg
    14461 agcacaggtg cccatggaat ccattagttt ggagctagtg atggctgtga gctgcctaag
    14521 tgaatgctgt ctcaactcaa cgtccttcta gtcctctgca agagtcccac ctactcttac
    14581 aagctgagcc atttctccaa ccccaaagtt gtttcttttc taagtagagt tttcactgtt
    14641 ttatatgtat tttactttat gcatctatgt ggctttgctt gcaggttatt gtgcccctgc
    14701 tttcaggtct agtgccccag gaaataagac aaggatgtca tacccctcag gactggagtt
    14761 agagacagtt agttgtgagt caccatatgg gaatcgaagc caggtcctct ggaagagtag
    14821 ctggtgttct taacagctga gccatcactc cagctgccga tatgcttccc tttaaaatat
    14881 cctaggatta aaaatatata catatggagc acaaagccct ggccatctct gagctcacat
    14941 cacagaaggg agagatcatt gtaatttaac caacgcctct tgtgaaacaa accaggcaat
    15001 ctaattcagt cgggcttctc accattttct gccacgtaac ctaaagaaac agggaagcct
    15061 aacactcgaa gatgtcagtg ttctggattc ctcgggacaa tggaagagag acatggtggg
    15121 atgagtgatc agcctctcaa ggactgaatg gtggcaagtt tcctggaagt gcccccatta
    15181 tttatttatt tatttgcttt acatcccagt caacctcccc tcacaagtcc ctcccattac
    15241 tccctccctt ctcctcatag tacagtcact tgacaacatt cacttaggtg atacccaaag
    15301 tgagtgctac caccccatct tgggacatca agtcatagaa ggactaatca tagcctctct
    15361 cactgagccc aaccaggcag tccagctaga ggaaggggat ccaagaacag gtgggggctc
    15421 acataaagac caggcttcaa tctgctacaa atgtgtagga agcgtaggtc aagccctgca
    15481 cgctctttgg tgggtggttc agtctctgtg agcccccatg ggcccaggtt agttgactct
    15541 gtaggtcttc ttgtggtgtc cttgctcact tctatccctc acttttccac aggactccca
    15601 aacactgctg agccatctta ccagccccct agaagtgttg tttattgtta cataaaaggc
    15661 acttttagca agt
//
