LOCUS       Exported               16206 bp ds-DNA     circular SYN 21-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 22, 2020 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
COMMENT     Assemble of: 
            Fragment  : pROSA26-TG : AscI at 5716 to PacI at 1092.
            Fragment  : pBigTB-ERT2CreERT2 : PacI at 4131 to AscI at 1254.
            Assembled on 06.10.2013
FEATURES             Location/Qualifiers
     source          1..16206
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..1104
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
                     /note="color: #ff99cc"
     misc_feature    1121..1224
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
                     /note="This feature has 2 segments:
                        1: 1121 .. 1186 / #------
                        2: 1187 .. 1224 / #a6acb3"
     promoter        1342..1671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #99ccff; direction: RIGHT"
     rep_origin      1522..1657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        1719..1766
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1785..2183
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #00ccff"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2341..2462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #99ccff"
     enhancer        2489..2868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #99cc00"
     promoter        2871..3146
                     /label=chicken beta-actin promoter
                     /note="color: #99cc00; direction: RIGHT"
     intron          3147..4155
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     regulatory      4221..4230
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             4230..5165
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /note="color: #339966"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5175..6197
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #008000"
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6210..7145
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /note="color: #339966"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     polyA_signal    7183..7407
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    7457..11737
                     /label=ROSA26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
                     /note="color: #ff99cc"
     promoter        11778..12271
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #cc99ff; direction: RIGHT"
     CDS             12298..12867
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /note="color: #993366"
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12944..13009
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     polyA_signal    13083..13307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(13375..13393)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(13414..13430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    13438..13454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(13462..13492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 13462 .. 13468 / #ffffff / -10
                        2: 13469 .. 13486 / #ffffff
                        3: 13487 .. 13492 / #ffffff / -35"
     protein_bind    13507..13528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(13816..14404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(14575..15435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 14575 .. 15366 / #ccffcc
                        2: 15367 .. 15435 / #ccffcc / signal sequence
                     Cleavage site after base 15366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15436..15540)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(15566..16021)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     16163..16179
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        join(16189..16206,1)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 ggcgaattgg agctccaccg cggcaggccc tccgagcgtg gtggagccgt tctgtgagac
       61 agccgggtac gagtcgtgac gctggaaggg gcaagcgggt ggtgggcagg aatgcggtcc
      121 gccctgcagc aaccggaggg ggagggagaa gggagcggaa aagtctccac cggacgcggc
      181 catggctcgg gggggggggg gcagcggagg ascgcttccg gccgacgtct cgtcgctgat
      241 tggcttyttt tcctcccgcc gtgtgtgaaa acacaaatgg cgtgttttgg ttggcgtaag
      301 gcgcctgtca gttaacggca gccggagtgc gcagccgccg gcagcctcgc tctgcccact
      361 gggtggggcg ggaggtaggt ggggtgaggc gagctgnacg tgcgggcgcg gtcggcctct
      421 ggcggggcgg gggaggggag ggagggtcag cgaaagtagc tcgcgcgcga gcggccgccc
      481 accctcccct tcctctgggg gagtcgtttt acccgccgcc ggccgggcct cgtcgtctga
      541 ttggctctcg gggcccagaa aactggccct tgccattggc tcgtgttcgt gcaagttgag
      601 tccatccgcc ggccagcggg ggcggcgagg aggcgctccc aggttccggc cctcccctcg
      661 gccccgcgcc gcagagtctg gccgcgcgcc cctgcgcaac gtggcaggaa gcgcgcgctg
      721 ggggcgggga cgggcagtag ggctgagcgg ctgcggggcg ggtgcaagca cgtttccgac
      781 ttgagttgcc tcaagagggg cgtgctgagc cagacctcca tcgcgcactc cggggagtgg
      841 agggaaggag cgagggctca gttgggctgt tttggaggca ggaagcactt gctctcccaa
      901 agtcgctctg agttgttatc agtaagggag ctgcagtgga gtaggcgggg agaaggccgc
      961 acccttctcc ggagggggga ggggagtgtt gcaatacctt tctgggagtt ctctgctgcc
     1021 tcctggcttc tgaggaccgc cctgggcctg ggagaatccc ttgccccctc ttcccctcgt
     1081 gatctgcaac tccagtcttt ctagttaatt aagggatctg tagggcgcag tagtccaggg
     1141 tttccttgat gatgtcatac ttatcctgtc cctttttttt ccacagctcg cggttgagga
     1201 caaactcttc gcggtctttc cagtggggat cgacggtatc gtagagtcga ggccgctcta
     1261 gaactagttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1321 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     1381 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     1441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     1501 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     1621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     1681 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     1741 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt
     1801 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     1861 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     1921 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     1981 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     2041 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     2101 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     2161 ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg acacgtgcta
     2221 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     2281 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     2341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2461 tatcatgtct gctagcatcg ataccgtcga cattgattat tgactagtta ttaatagtaa
     2521 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2581 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2641 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta
     2701 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2761 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2821 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg tcgaggtgag
     2881 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     2941 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag
     3001 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     3061 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     3121 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc
     3181 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     3241 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg
     3301 tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg
     3361 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg
     3421 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt
     3481 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac
     3541 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc
     3601 gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     3661 tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     3721 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     3781 cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     3841 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg gagccgaaat
     3901 ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg gcgccggcag
     3961 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct
     4021 ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg
     4081 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     4141 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     4201 aaagaattcc cgggtgagcc gccaccatgg ctggagacat gagagctgcc aacctttggc
     4261 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
     4321 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
     4381 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
     4441 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
     4501 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     4561 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     4621 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     4681 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     4741 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     4801 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     4861 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     4921 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     4981 tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca
     5041 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     5101 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     5161 ccacagctgt cgacaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg
     5221 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt
     5281 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt
     5341 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc
     5401 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc
     5461 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc
     5521 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg
     5581 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata
     5641 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg
     5701 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg
     5761 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa
     5821 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc
     5881 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa
     5941 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg
     6001 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag
     6061 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg
     6121 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc
     6181 tgctggaaga tggcgatctc gagccatctg ctggagacat gagagctgcc aacctttggc
     6241 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
     6301 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
     6361 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
     6421 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
     6481 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     6541 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     6601 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     6661 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     6721 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     6781 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     6841 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     6901 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     6961 tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca
     7021 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     7081 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     7141 ccacagcttg atagcggccg cgagctcgct gatcagcctc gactgtgcct tctagttgcc
     7201 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7261 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7321 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7381 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     7441 tcctctagtt ggcgcgccct agaagatggg cgggagtctt ctgggcaggc ttaaaggcta
     7501 acctggtgtg tgggcgttgt cctgcagggg aattgaacag gtgtaaaatt ggagggacaa
     7561 gacttcccac agattttcgg ttttgtcggg aagtttttta ataggggcaa ataggaaaat
     7621 ggaggatagg agtcatctgg ggtttatgca gcaaaactac aggtatattg cttgtgatcc
     7681 gcctcggaga tttccatgag gagataaaga catgtcaccc gagtttatac tctcctgctt
     7741 agatcctact acagtatgaa atacagtgty gcgaggtaga ctatgtaagc agatttaatc
     7801 attttaaaga gcccagtact tcatatccat ttctcccgct ccttctgcag ccttatcaaa
     7861 aggtatttag aacactcatt ttagccccat tttcatttat tatactggct tatccaaccc
     7921 ctagacagag cattggcatt ttccctttcc tgatcttaga agtctgatga ctcatgaaac
     7981 cagacagatt agttacatac accacaaatc gaggctgtag ctggggcctc aacactgcag
     8041 ttcttttata actccttagt acactttttg ttgatccttt gccttgatcc ttaattttca
     8101 gtgtctatca cctctcccgt caggtggtgt tccacatttg ggcctattct cagtccaggg
     8161 agttttacaa caatagatgt attgagaatc caacctaaag cttaactttc cactcccatg
     8221 aatgcctctc tcctttttct ccattataac tgagctatwa ccattaatgg tttcaggtgg
     8281 atgtctcctc ccccaatata cctgatgtat ctacatattg ccaggctgat attttaagac
     8341 atwaaaggta tatttcatta ttgagccaca tggtattgat tactgctact aaaattttgt
     8401 cattgtacac atctgtaaaa ggtggttcct tttggaatgc aaagttcagg tgtttgttgt
     8461 ctttcctgac ctaaggtctt gtgagcttgt attttttcta tttaagcagt gctttctctt
     8521 ggactggctt gactcatggc attctacacg ttattgctgg tctaaatgtg attttgccaa
     8581 gcttcttcag gacctataat tttgcttgac ttgtagccaa acacaagtaa aatgattaag
     8641 caacaaatgt atttgtgaag cttggttttt aggttgttgt gttgtgtgtg cttgtgctct
     8701 ataataatac tatccagggg ctggagaggt ggctcggagt tcaagagcac agactgctct
     8761 tccagaagtc ctgagttcaa ttcccagcaa ccacatggtg gctcacaacc atctgtaatg
     8821 ggatctgatg ccctcttctg gtgtgtctga agaccacaag tgtattcaca ttaaataaat
     8881 aatcctcctt cttcttcttt tttttttttt aaagagaatw ctgtctccag tagaattact
     8941 gaagtaatga aatactttgt gtttgttcca atatggwagc caataatcaa atactcttwa
     9001 gcactggaaa tgtaccaagg aactatttta tttaagtgwa ctgtggacag aggagccata
     9061 actgcagact tgtgggatac agaagaccaa tgcagactta atgtcttttc tcttacacta
     9121 agcaataaag aaataaaaat tgaacttcta gtatcctatt tgttaaactg ctagctttac
     9181 taacttttgt gcttcatcta tacaaagctg aaagctaagt ctgcagccat tactaaacat
     9241 gaaagcaagt aatgataatt ttggatttca aaaatgtagg gccagagttt agccagccag
     9301 tggtggtgct tgcctttatg ccttaatccc agcactctgg aggcagagac aggcagatct
     9361 ctgagtttga gcccagcctg gtctacacat caagttctat ctaggatagc caggaataca
     9421 cacagaaacc ctgttgggga ggggggctct gagatttcat aaaattataa ttgaagcatt
     9481 ccctaatgag ccactatgga tgtggctaaa tccgtctacc tttctgatga gatttgggta
     9541 ttattttttc tgtctctgct gttggttggg tcttttgaca ctgtgggctt tcttaaagcc
     9601 tccttccctg ccatgtggtc tcttgtttgc tactaacttc ccatggctta aatggcatgg
     9661 ctttttgcct tctaagggca gctgctgagw tttgcagcct gatttccagg gtggggttgg
     9721 gaaatctttc aaacactaaa attgtccttt aatttttttt taaaaaatgg gttatataat
     9781 aaacctcata aaatagttat gaggagtgag gtggactaat attaatgagt ccctccccta
     9841 taaaagagct attaaggctt tttgtcttat actaactttt tttttaaatg tggtatcttt
     9901 agaaccaagg gtcttagagt tttagtatac agaaactgtt gcatcgctta atcagatttt
     9961 ctagtttcaa atccagagaa tccaaattct tcacagccaa agtcaaatta agaatttctg
    10021 actttaatgt tatttgctac tgtgaatata aaatgatagc ttttcctgag gcagggtctc
    10081 actatgtatc tctgcctgat ctgcaacaag atatgtagac taaagttctg cctgcttttg
    10141 tctcctgaat actaaggtta aaatgtagta atacttttgg aacttgcagg tcagattctt
    10201 ttatagggga cacactaagg gagcttgggt gatagttggt aaatgtgttt aagtgatgaa
    10261 aacttgaatt attatcaccg caacctactt tttaaaaaaa aaagccaggc ctgttagagc
    10321 atgctaaggg atccctagga cttgctgagc acacaagagt agtacttggc aggctcctgg
    10381 tgagagcata tttcaaaaaa caaggcagac aaccaagaaa ctacagtaag gttacctgtc
    10441 tttaaccatc tgcatataca cagggatatt aaaatattcc aaataatatt tcattcaagt
    10501 tttcccccat caaattggga catggatttc tccggtgaat aggcagagtt ggaaactaaa
    10561 caaatgttgg ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag cccatgagat
    10621 acagaacaaa gctgctattt cgaggtctct tggttatact cagaagcact tctttgggtt
    10681 tccctgcact atcctgatca tgtgctaggc ctwccttagg ctgattgttg ttcaaataac
    10741 ttaagtttcc tgtcaggtga tgtcatatga tttcatatat caaggcaaaa catgttatat
    10801 atgttaaaca tttgkactta atgtgaaagt taggtctttg tgggttttga ttttaatttc
    10861 aaaacctgag ctaaataagt cattttacat gtcttacatt tggtgaattg tatattgtgg
    10921 tttgcaggca agactctctg acctagtaac cctcctatag agcactttgc tgggtcacaa
    10981 gtctaggagt caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttat
    11041 atgttggagg acatgtttat ccagaagata ttcaggacta tttttgactg ggctaaggaa
    11101 ttgattctga ttagcactgt tagtgagcat tgagtggcct ttaggcttga attggagtca
    11161 cttgtatatc tcaaataatg ctggcctttt ttwaaaagcc cttgttcttt atcaccctgt
    11221 tttctacata atttttgttc aaagaaatac ttgtttggat ctccttttga caacaatagc
    11281 atgttttcaa gccatatttt ttttcctttt tttttttttt tttggttttt cgagacaggg
    11341 tttctctgta tagccctggc tgtcctggaa ctcactttgt agaccaggct ggcctcgaac
    11401 tcagaaatcc gcctgcctct gcctcctgag tgccgggatt aaaggcgtgc accaccacgc
    11461 ctggctaagt tggatatttt gtatataact ataaccaata ctaactccac tgggtggatt
    11521 tttaattcag tcagtagtct taagtggtct ttattggccc ttattaaaat ctactgttca
    11581 ctctaacaga ggctgttgga ctagtggsac taagcaactt cctacggata tactagcaga
    11641 taagggtcag ggatagaaac tagtctagcg ttttgtatac ctaccagctt atactacctt
    11701 gttctgatag aaatatttag gacatctagc ttatcgatcc gtcgacggta tcgataagct
    11761 tgatatcgaa ttctaccggg taggggaggc gcttttccaa ggcagtctga gcatgcgctt
    11821 agcagccccg ctggcacttg gcgctacaca agtggcctyt ggcctcgcac acattccaca
    11881 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctwct
    11941 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtsagg acgtgacaaa
    12001 tggaagtagc acgtctcact agtctcgtca gatggacagc accgctgagc aatggaagcg
    12061 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    12121 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    12181 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    12241 gttctcctct tcctcatctc cgggcctttc gacctgcagg tcctcgccat ggatcctgat
    12301 gatgttgtta ttcttctaat cttttgtatg gaaaactttt cttcgtacca cgggactaaa
    12361 cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg tacacaagga
    12421 aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga cgctgcggga
    12481 tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt caaagtgacg
    12541 tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac tattaagaaa
    12601 gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga agagtttatc
    12661 aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc tgaggggagt
    12721 tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt agaacttgag
    12781 attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta tatggctcaa
    12841 gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact tctgtggtgt
    12901 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    12961 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    13021 tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct gatcagcctc
    13081 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    13141 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    13201 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    13261 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    13321 aagaaccagc tggggctcga cctcgagggg gggcccggta cccagctttt gttcccttta
    13381 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    13441 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    13501 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    13561 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    13621 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    13681 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    13741 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    13801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    13861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    13921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    13981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    14041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    14101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    14161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    14221 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    14281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    14341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    14401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    14461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    14521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    14581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    14641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    14701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    14761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    14821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    14881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    14941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    15001 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    15061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    15121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    15181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    15241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    15301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    15361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    15421 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    15481 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    15541 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
    15601 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
    15661 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
    15721 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
    15781 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
    15841 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
    15901 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
    15961 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
    16021 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    16081 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    16141 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
    16201 ctatag
//
