LOCUS       prk5_MFSD12_tev3xflag3x 6373 bp ds-DNA     circular     06-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        4634..4902
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     feature         4979..5122
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2641..4081
                     /label="MFSD12 (recoded)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          4863..4882
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4979..5001)
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4381..4524
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     origin          683..1302
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4081..4302
                     /label="TEV-3XHA-3XFLAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1719..2295
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        2265..2384
                     /label="CMV2_promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            join(6041..6373,1..528)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        5971..5999
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        1611..1640
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2532..2549
                     /label="Sp6_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2296..2315
                     /label="pCEP_fwd_primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5790..5812
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2298..2322
                     /label="LNCX_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     origin          5216..5522
                     /label="f1_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1654..1676
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(4970..4986)
                     /label="M13_forward20_primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     regulatory      complement(4622..4837)
                     /label="SV40_enhancer"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      1798..2085
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4481..4500
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4616..4636)
                     /label="pBABE_3_primer"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2253..2322
                     /label="CMV_promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     origin          4801..4878
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2252..2272
                     /label="CMV_fwd_primer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
       61 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
      121 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
      181 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
      241 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
      301 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
      361 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
      421 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
      481 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
      541 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
      601 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
      661 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
      721 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
      781 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
      841 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
      901 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
      961 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     1021 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     1081 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     1141 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     1201 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     1261 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     1321 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
     1381 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
     1441 AGCGAGTCAG TGAGCGAGGA AGCGGAAGag cgcccaatac gcaaaccgcc tctccccgcg
     1501 cgttggccga ttcattaatg CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
     1561 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT
     1621 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC
     1681 AGCTATGACA TGATTACGAA TTAATTCGAG CTCGCCCGAC ATTGATTATT GACTAGTTAT
     1741 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA
     1801 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
     1861 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
     1921 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
     1981 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
     2041 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG
     2101 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA
     2161 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT
     2221 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
     2281 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA
     2341 CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGCGG CCGGGAACGG
     2401 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT
     2461 AGGCCCACCC CCTTGGCTTC GTTAGAACGC GGCTACAATT AATACATAAC CTTATGTATC
     2521 ATACACATAC GATTTAGGTG ACACTATAGA ATAACATCCA CTTTGCCTTT CTCTCCACAG
     2581 GTGTCCACTC CCAGGTCCAA CTGCACCTCG GTTCTATCGA TTGAATTCGT CGACGCCACC
     2641 ATGGGTCCTG GTCCACCTGC CGCTGGCGCG GCTCCATCTC CACGGCCCCT GAGTCTCGTA
     2701 GCCCGGTTGT CCTATGCCGT GGGCCATTTC CTTAATGACC TTTGTGCCAG TATGTGGTTT
     2761 ACCTACTTGT TGCTTTATCT CCATAGCGTG AGAGCTTATT CTTCTCGAGG AGCGGGTTTG
     2821 CTCCTGTTGC TGGGCCAAGT TGCAGATGGC CTGTGCACCC CTCTTGTGGG TTATGAGGCC
     2881 GATAGAGCGG CAAGTTGCTG TGCAAGGTAT GGTCCGCGAA AGGCATGGCA TTTGGTTGGC
     2941 ACCGTATGCG TACTTCTTTC TTTCCCATTT ATCTTCTCTC CATGTCTCGG CTGCGGCGCA
     3001 GCCACCCCCG AATGGGCTGC TTTGTTGTAT TACGGACCTT TCATCGTAAT ATTCCAATTC
     3061 GGCTGGGCGT CAACGCAAAT TAGCCATCTC AGTCTCATTC CGGAACTTGT TACTAATGAT
     3121 CACGAAAAAG TAGAGCTTAC CGCGTTGCGC TATGCCTTCA CCGTTGTAGC CAACATAACG
     3181 GTATACGGCG CTGCCTGGCT TCTCTTGCAC CTCCAGGGGA GCTCAAGGGT CGAGCCAACG
     3241 CAGGATATTT CAATATCAGA CCAGCTCGGT GGTCAGGATG TTCCGGTGTT CCGGAATCTG
     3301 AGTTTGCTCG TCGTCGGAGT TGGAGCTGTC TTCAGCCTTC TTTTCCACCT TGGAACAAGG
     3361 GAACGCAGAC GGCCTCACGC TGAAGAGCCA GGTGAGCACA CTCCGCTTCT GGCCCCTGCT
     3421 ACAGCGCAAC CCTTGCTTCT CTGGAAGCAT TGGCTGAGAG AACCCGCTTT CTATCAAGTG
     3481 GGCATACTGT ATATGACAAC GAGGCTTATT GTGAATCTGA GTCAAACCTA TATGGCCATG
     3541 TATTTGACAT ATTCTCTTCA CTTGCCTAAA AAGTTCATCG CCAcAATTCC GCTTGTTATG
     3601 TATCTGAGTG GTTTCTTGAG TAGCTTTCTG ATGAAGCCGA TCAACAAGTG CATTGGACGG
     3661 AACATGACGT ACTTCAGTGG CCTTTTGGTC ATCCTGGCTT TTGCAGCATG GGTTGCTCTT
     3721 GCGGAGGGCC TGGGAGTAGC AGTGTATGCG GCTGCTGTTC TGTTGGGGGC CGGGTGTGCA
     3781 ACAATCCTCG TGACGTCCCT TGCGATGACG GCAGATCTGA TTGGGCCTCA CACGAAcTCC
     3841 GGAGCCTTCG TTTACGGTTC TATGTCCTTC TTGGACAAGG TTGCTAATGG GCTTGCCGTG
     3901 ATGGCAATTC AATCCCTTCA CCCGTGCCCT TCTGAGTTGT GCTGCAGAGC GTGTGTGTCC
     3961 TTTTATCATT GGGCTATGGT CGCTGTGACG GGTGGAGTAG GGGTGGCAGC AGCCCTCTGC
     4021 CTCTGTAGTC TTTTGCTGTG GCCGACTAGG CTTCGCCGCT GGGACCGGGA CGCCCGCCCG
     4081 GCGGCCGCAG AAAACCTGTA CTTCCAAGGT CCCGTTTACC CATACGATGT TCCTGACTAT
     4141 GCGGGCTATC CCTATGACGT CCCGGACTAT GCAGGATCCT ATCCATATGA CGTTCCAGAT
     4201 TACGCTCCGG CCGCCGGATC CGGATCCGCA GGCGACTACA AAGACCATGA CGGTGATTAT
     4261 AAAGATCATG ACATTGATTA CAAGGATGAC GATGACAAGT AATAATAAGC GGCCGCAGGA
     4321 GGTGGAGGTT ACCCCTATGA TGTGCCTGAC TACGCATGAT AAGGATCCCC AGCTTGGCCG
     4381 CCATGGCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA
     4441 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC
     4501 AATGTATCTT ATCATGTCTG GATCGGGAAT TAATTCGGCG CAGCACCATG GCCTGAAATA
     4561 ACCTCTGAAA GAGGAACTTG GTTAGGTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT
     4621 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC
     4681 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA
     4741 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC
     4801 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT
     4861 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA
     4921 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTGTTAA CAGCTTGGCA CTGGCCGTCG
     4981 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC
     5041 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC
     5101 AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT
     5161 GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT
     5221 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
     5281 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
     5341 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
     5401 CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT
     5461 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
     5521 AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
     5581 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
     5641 AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
     5701 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
     5761 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC
     5821 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
     5881 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     5941 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     6001 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     6061 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     6121 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     6181 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     6241 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     6301 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     6361 AGAAAAGCAT CTT
//