LOCUS       Exported                8331 bp ds-DNA     circular SYN 25-MAY-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 25, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="synthetic DNA construct"
                     /label=dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)
                     /note="dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)"
     source          2576..3160
                     /organism="synthetic DNA construct"
                     /label=dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)
                     /note="dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)"
     source          3211..7773
                     /label=dnas_title=AsCpf1
                     /note="dnas_title=AsCpf1"
     source          3212..7408
                     /organism="synthetic DNA construct"
                     /label=/dnas_title=pYPQ166
                     /note="/dnas_title=pYPQ166"
     source          7409..7456
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pYPQ166"
     promoter        1..138
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=Streptomycin/Spectinomycin adenyltransferase
                     /note="SpnR
                     vntifkey=4
                     ApEinfo_revcolor=pink
                     invitrogen=2750000
                     ApEinfo_fwdcolor=pink
                     dnas_title=SpnR
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="pUC origin
                     vntifkey=33
                     ApEinfo_revcolor=pink
                     invitrogen=2530000
                     ApEinfo_fwdcolor=pink
                     dnas_title=pUC origin
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     terminator      2319..2362
                     /note="rrnB T1 terminatior
                     vntifkey=43
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=rrnB T1 terminatior 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_recomb     2461..2560
                     /label=attL1
                     /note="attL1
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2670000
                     ApEinfo_fwdcolor=#00ff40
                     dnas_title=attL1
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             2576..3160
                     /codon_start=1
                     /label=hAID*Delta
                     /translation="MDSLLMNRREFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFISWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHGRTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRT"
     misc_feature    3161..3208
                     /label=XTEN linker
                     /note="XTEN linker"
     CDS             3212..7456
                     /codon_start=1
                     /product="iSpyMacCas9, maize codon optimised "
                     /label=ziSpyMacCas9 fusion protein
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9 /vntifkey=4"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEIQTVGQNGGLFDDNPKSPLEVTPSKLVP
                     LKKELNPKKYGGYQKPTTAYPVLLITDTKQLIPISVMNKKQFEQNPVKFLRDRGYQQVG
                     KNDFIKLPKYTLVDIGDGIKRLWASSKEIHKGNQLVVSKKSQILLYHAHHLDSDLSNDY
                     LQNHNQQFDVLFNEIISFSKKCKLGKEHIQKIENVYSNKKNSASIEELAESFIKLLGFT
                     QLGATSPFNFLGVKLNQKQYKGKKDYILPCTEGTLIRQSITGLYETRVDLSKIGEDKRP
                     AATKKAGQAKKKK"
     sig_peptide     3215..3280
                     /label=3xFLAG
     misc_feature    3287..3307
                     /label=SV40 NLS
     CDS             3332..6625
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE"
     misc_feature    3356..3358
                     /label=D10A
     primer_bind     complement(3966..3991)
                     /label=166-R221K-Rev
     misc_feature    3989..3991
                     /label=R221K
     primer_bind     3992..4013
                     /label=166-R221K-For
     primer_bind     complement(4486..4510)
                     /label=166-N394K-Rev
     misc_feature    4508..4510
                     /label=N394K
     primer_bind     4511..4532
                     /label=166-N394K-For
     misc_feature    6475..7408
                     /label=zMacCas9-C (gBlock)
     CDS             6626..7408
                     /codon_start=1
                     /label=macCas9z-C-terminus
                     /translation="IQTVGQNGGLFDDNPKSPLEVTPSKLVPLKKELNPKKYGGYQKPT
                     TAYPVLLITDTKQLIPISVMNKKQFEQNPVKFLRDRGYQQVGKNDFIKLPKYTLVDIGD
                     GIKRLWASSKEIHKGNQLVVSKKSQILLYHAHHLDSDLSNDYLQNHNQQFDVLFNEIIS
                     FSKKCKLGKEHIQKIENVYSNKKNSASIEELAESFIKLLGFTQLGATSPFNFLGVKLNQ
                     KQYKGKKDYILPCTEGTLIRQSITGLYETRVDLSKIGED"
     misc_feature    7409..7456
                     /label=nucleoplasmin NLS
     misc_feature    7466..7759
                     /label=UGI-NLS
                     /note="UGI-NLS"
     misc_feature    7779..8056
                     /label=NOS term
                     /note="NOS term
                     vntifkey=21
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#f5010a
                     dnas_title=NOS term
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_feature    complement(7823..7846)
                     /label=PCR_primer
                     /note="PCR_primer
                     Nos-term-R2"
     misc_recomb     8078..8201
                     /label=attR5
                     /note="attR5
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2703000
                     ApEinfo_fwdcolor=#00ff00
                     dnas_title=attR5
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     promoter        8330..8331
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgga
     2581 ctcacttttg atgaataggc gggagttctt gtaccagttt aagaacgtgc ggtgggcgaa
     2641 aggtcggagg gaaacctacc tttgctatgt ggttaagaga cgcgacagcg caacatcctt
     2701 ctccttggac tttggatacc ttcgcaataa gaatgggtgt catgtcgaac tgttgtttct
     2761 ccggtatatt agcgattggg acctggaccc cggcaggtgt tacagggtga catggttcat
     2821 atcgtggtct ccatgctatg actgcgctag gcatgttgct gattttctta ggggcaatcc
     2881 aaacctgagt cttcgcattt tcacggcgcg gctgtatttt tgtgaggacc gcaaggcaga
     2941 gcccgaggga ttgcgcaggc ttcatagggc aggagtccaa atagctatca tgactttcaa
     3001 agactatttt tactgttgga acacgttcgt cgagaaccac ggacggacgt ttaaggcttg
     3061 ggaaggactg catgaaaaca gtgtcaggct ctcaaggcaa ctccgccgca tacttttgcc
     3121 tttgtacgag gttgacgacc tgcgcgacgc atttcggact tcaggttctg agacccctgg
     3181 cacaagtgag tcagcaacac ccgagtccac catggattac aaggaccacg acggggatta
     3241 caaggaccac gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag
     3301 gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgctat
     3361 tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa
     3421 gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct
     3481 cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg
     3541 gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc
     3601 gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa
     3661 gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa
     3721 gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct
     3781 ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga
     3841 gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac
     3901 gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat
     3961 cctgtccgct aggctctcga agtctcggaa gctcgagaac ctgatcgccc agctgccggg
     4021 cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa
     4081 cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata
     4141 cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct
     4201 cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga
     4261 gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga
     4321 tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt
     4381 cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga
     4441 gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt
     4501 gaagctcaag cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc
     4561 acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc
     4621 tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta
     4681 cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga
     4741 gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt
     4801 catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca
     4861 ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac
     4921 cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct
     4981 cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa
     5041 gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct
     5101 ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga
     5161 gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat
     5221 gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct
     5281 caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg
     5341 ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg
     5401 caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc
     5461 tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc
     5521 agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat
     5581 gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca
     5641 gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct
     5701 ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct
     5761 ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa
     5821 caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc
     5881 cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc
     5941 ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct
     6001 gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct
     6061 ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt
     6121 tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg
     6181 cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca
     6241 gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc
     6301 ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg
     6361 ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa
     6421 ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac
     6481 tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga
     6541 gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca
     6601 agtgaatatc gtcaagaaga cagagataca aacggttggt cagaacgggg gtcttttcga
     6661 tgataaccca aaatccccgc ttgaggtgac cccttcaaag ctggtgccac tcaaaaagga
     6721 attgaatcca aaaaagtatg gcggatacca aaagcctaca acggcgtatc ccgtcctctt
     6781 gattacggat acaaagcaac ttattcctat atccgttatg aataaaaaac agttcgagca
     6841 gaaccctgtt aagttcctgc gggatagagg ctatcaacaa gtgggcaaga acgattttat
     6901 taagcttcct aaatatacgc tcgtggatat tggagacggc ataaaaagac tttgggcttc
     6961 ctcgaaagaa atacataagg gcaaccaact cgtcgtctca aaaaagtctc agatccttct
     7021 ttatcatgcg caccaccttg attcagacct ctcaaacgat tatcttcaga accataatca
     7081 gcagtttgac gtgctcttca acgagataat ttcattctct aaaaagtgca agctcgggaa
     7141 ggagcacata cagaaaatcg aaaacgtcta ttctaacaaa aagaactcag cctccataga
     7201 ggagttggca gaatcattca taaagctcct gggctttact cagctgggcg ccacatcacc
     7261 gtttaacttt ctgggagtca aactcaatca aaagcagtat aagggcaaaa aggactatat
     7321 tctcccctgt accgaaggaa ctctcatcag gcaatccatt acagggttgt acgaaacaag
     7381 ggtcgacctc tcaaaaatag gagaggacaa gcggccagcg gcgacgaaga aggcggggca
     7441 ggcgaagaag aagaagggag acggatcagg cgggtcaaca aacctcagtg acatcataga
     7501 gaaggaaact ggtaagcaac tggttattca agagagcatt ctgatgctcc ctgaggaagt
     7561 cgaggaagtt ataggaaaca agcctgagag cgatatactc gtgcatacgg cgtatgacga
     7621 gagcacggat gaaaatgtga tgctcttgac cagcgatgcg ccagaatata aaccctgggc
     7681 actggttatt caggactcta acggggaaaa taaaatcaag atgctctcag gtggctcccc
     7741 aaagaagaaa cgcaaggttt agtggcggcc gccgacgtcc gatcgttcaa acatttggca
     7801 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     7861 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     7921 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     7981 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     8041 gatcccccct cgacagcttc cggaaagggc gaattcgcaa ctttgtatac aaaagttgaa
     8101 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     8161 actacataat actgtaaaac acaacatatc cagtcactat gccatccagc tgatatcccc
     8221 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     8281 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct c
//
