LOCUS       Exported                8817 bp ds-DNA     circular SYN 25-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 25, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pYPQ256 (pcoCas9n-PmCDA-UGI)"
     source          2578..8259
                     /note="/dnas_title=AsCpf1"
     source          2579..6775
                     /organism="synthetic DNA construct"
                     /label=/dnas_title=pYPQ166
                     /note="/dnas_title=pYPQ166"
     source          6776..6823
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pYPQ166"
     promoter        1..138
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=Spn promoter
                     /vntifkey=30"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=SpnR
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=SpnR
                     /invitrogen=2750000
                     /vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=pUC origin
                     /invitrogen=2530000
                     /vntifkey=33"
     terminator      2319..2362
                     /label=rrnB T1 terminatior
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=rrnB T1 terminatior
                     /vntifkey=43"
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2560
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attL1
                     /invitrogen=2670000
                     /vntifkey=86"
     primer_bind     complement(2548..2599)
                     /label=attL1-R1-Rec
     CDS             2579..6823
                     /codon_start=1
                     /product="iSpyMacCas9, maize codon optimised "
                     /label=ziSpyMacCas9 fusion protein
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9 /vntifkey=4"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEIQTVGQNGGLFDDNPKSPLEVTPSKLVP
                     LKKELNPKKYGGYQKPTTAYPVLLITDTKQLIPISVMNKKQFEQNPVKFLRDRGYQQVG
                     KNDFIKLPKYTLVDIGDGIKRLWASSKEIHKGNQLVVSKKSQILLYHAHHLDSDLSNDY
                     LQNHNQQFDVLFNEIISFSKKCKLGKEHIQKIENVYSNKKNSASIEELAESFIKLLGFT
                     QLGATSPFNFLGVKLNQKQYKGKKDYILPCTEGTLIRQSITGLYETRVDLSKIGEDKRP
                     AATKKAGQAKKKK"
     sig_peptide     2582..2647
                     /label=3xFLAG
     misc_feature    2654..2674
                     /label=SV40 NLS
     CDS             2699..5992
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE"
     misc_feature    2723..2725
                     /label=D10A
     primer_bind     complement(3333..3358)
                     /label=166-R221K-Rev
     misc_feature    3356..3358
                     /label=R221K
     primer_bind     3359..3380
                     /label=166-R221K-For
     primer_bind     complement(3853..3877)
                     /label=166-N394K-Rev
     misc_feature    3875..3877
                     /label=N394K
     primer_bind     3878..3899
                     /label=166-N394K-For
     misc_feature    5842..6775
                     /label=zMacCas9-C (gBlock)
     CDS             5993..6775
                     /codon_start=1
                     /label=macCas9z-C-terminus
                     /translation="IQTVGQNGGLFDDNPKSPLEVTPSKLVPLKKELNPKKYGGYQKPT
                     TAYPVLLITDTKQLIPISVMNKKQFEQNPVKFLRDRGYQQVGKNDFIKLPKYTLVDIGD
                     GIKRLWASSKEIHKGNQLVVSKKSQILLYHAHHLDSDLSNDYLQNHNQQFDVLFNEIIS
                     FSKKCKLGKEHIQKIENVYSNKKNSASIEELAESFIKLLGFTQLGATSPFNFLGVKLNQ
                     KQYKGKKDYILPCTEGTLIRQSITGLYETRVDLSKIGED"
     misc_feature    6776..6823
                     /label=nucleoplasmin NLS
     primer_bind     complement(6796..6847)
                     /label=zCas9-R2-Rec
     primer_bind     6800..6851
                     /label=CDA-F1-Rec
     misc_feature    6833..8245
                     /label=GGGS-SH3-3XFLAG-PmCDA1-UGI
                     /note="GGGS-SH3-3XFLAG-PmCDA1-UGI"
     primer_bind     6849..6871
                     /label=Primer 5
                     /note="CDA-seq1"
     misc_feature    7040..7105
                     /label=3x FLAG
     misc_feature    7112..7735
                     /label=PmCDA
     primer_bind     7308..7331
                     /label=Primer 3
                     /note="CDA-seq2"
     sig_peptide     7739..7759
                     /label=SV40 NLS
     misc_feature    7766..8017
                     /label=UGI
     primer_bind     7789..7812
                     /label=Primer 4
                     /note="CDA-seq3"
     misc_feature    8265..8542
                     /label=NOS term
                     /note="/ApEinfo_fwdcolor=#f5010a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=NOS term
                     /vntifkey=21"
     misc_feature    complement(8309..8332)
                     /label=nonstandard type: PCR_primer
                     /note="Nos-term-R2"
     misc_recomb     8564..8687
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attR5
                     /invitrogen=2703000
                     /vntifkey=86"
     primer_bind     complement(8728..8744)
                     /label=M13 reverse primer
     promoter        8816..8817
                     /label=Spn promoter
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=Spn promoter
                     /vntifkey=30"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggattacaag gaccacgacg gggattacaa ggaccacgac attgattaca aggatgatga
     2641 tgacaagatg gctccgaaga agaagaggaa ggttggcatc cacggggtgc cagctgctga
     2701 caagaagtac tcgatcggcc tcgctattgg gactaactct gttggctggg ccgtgatcac
     2761 cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg atcggcattc
     2821 catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg ctgaggctac
     2881 gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca tctgctacct
     2941 gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc acaggctgga
     3001 ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct tcggcaacat
     3061 tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc ggaagaagct
     3121 cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg cccacatgat
     3181 taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata gcgatgttga
     3241 caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga accccattaa
     3301 tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt ctcggaagct
     3361 cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg ggaatctcat
     3421 tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg ctgaggacgc
     3481 caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc tggcccagat
     3541 cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg ccatcctcct
     3601 gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag cctccatgat
     3661 caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg tcaggcagca
     3721 gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct acgctgggta
     3781 cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga ttctggagaa
     3841 gatggacggc acggaggagc tgctggtgaa gctcaagcgc gaggacctcc tgaggaagca
     3901 gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc tgcacgctat
     3961 cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga agatcgagaa
     4021 gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca actcccgctt
     4081 cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg aggaggtggt
     4141 cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg acaagaacct
     4201 gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca cagtctacaa
     4261 cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt tcctgtctgg
     4321 ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg tcacggttaa
     4381 gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg agatctctgg
     4441 cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga agatcattaa
     4501 ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca ttgtgctgac
     4561 actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt acgcccatct
     4621 cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct gggggaggct
     4681 gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga tcctcgactt
     4741 cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg atgacagcct
     4801 cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact cgctgcacga
     4861 gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc agaccgtgaa
     4921 ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca tcgtcattga
     4981 gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg agaggatgaa
     5041 gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc acccggtgga
     5101 gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg gccgcgatat
     5161 gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg atcatatcgt
     5221 tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca ggtcggacaa
     5281 gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga tgaagaacta
     5341 ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata acctcacaaa
     5401 ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga ggcagctggt
     5461 cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga tgaacactaa
     5521 gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga agtcaaagct
     5581 cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca acaattacca
     5641 ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca agaagtaccc
     5701 aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga ggaagatgat
     5761 cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct actctaacat
     5821 tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga agcgccccct
     5881 catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg atttcgcgac
     5941 cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag agatacaaac
     6001 ggttggtcag aacgggggtc ttttcgatga taacccaaaa tccccgcttg aggtgacccc
     6061 ttcaaagctg gtgccactca aaaaggaatt gaatccaaaa aagtatggcg gataccaaaa
     6121 gcctacaacg gcgtatcccg tcctcttgat tacggataca aagcaactta ttcctatatc
     6181 cgttatgaat aaaaaacagt tcgagcagaa ccctgttaag ttcctgcggg atagaggcta
     6241 tcaacaagtg ggcaagaacg attttattaa gcttcctaaa tatacgctcg tggatattgg
     6301 agacggcata aaaagacttt gggcttcctc gaaagaaata cataagggca accaactcgt
     6361 cgtctcaaaa aagtctcaga tccttcttta tcatgcgcac caccttgatt cagacctctc
     6421 aaacgattat cttcagaacc ataatcagca gtttgacgtg ctcttcaacg agataatttc
     6481 attctctaaa aagtgcaagc tcgggaagga gcacatacag aaaatcgaaa acgtctattc
     6541 taacaaaaag aactcagcct ccatagagga gttggcagaa tcattcataa agctcctggg
     6601 ctttactcag ctgggcgcca catcaccgtt taactttctg ggagtcaaac tcaatcaaaa
     6661 gcagtataag ggcaaaaagg actatattct cccctgtacc gaaggaactc tcatcaggca
     6721 atccattaca gggttgtacg aaacaagggt cgacctctca aaaataggag aggacaagcg
     6781 gccagcggcg acgaagaagg cggggcaggc gaagaagaag aagggagacg acggaggtgg
     6841 cggtacagga gggggtgggt ccgctgagta tgtcagggcg ttgttcgact tcaatggaaa
     6901 cgacgaggaa gatctgcctt ttaaaaaggg agatattctc aggatcagag ataagccgga
     6961 agaacaatgg tggaacgctg aagactctga aggtaagaga ggtatgattc ttgtccccta
     7021 cgtcgagaag tattcgggtg actataaaga ccacgatgga gattataagg accacgatat
     7081 agattataag gatgatgatg ataagagcgg aatgaccgat gcagagtacg tcaggattca
     7141 tgagaaactt gacatctaca cgtttaagaa acagtttttc aacaacaaaa aatctgttag
     7201 tcaccgctgt tacgtgctgt tcgaattgaa acgcagaggt gagaggagag cctgcttttg
     7261 gggctatgcc gtcaacaagc cgcaaagcgg cacagaaagg ggcattcacg cggagatatt
     7321 tagcattaga aaggtcgagg aataccttcg ggataatccc gggcaattca ctatcaattg
     7381 gtactcttca tggtccccgt gtgcagattg cgctgaaaag atactggagt ggtataatca
     7441 agaactcaga ggaaacggtc acaccctcaa gatttgggct tgcaagcttt actacgagaa
     7501 aaatgcaagg aaccagatcg gcctctggaa cttgcgcgac aacggcgtgg ggttgaatgt
     7561 gatggtctcg gagcattacc agtgctgccg gaagatattc attcagtcgt cacataatca
     7621 attgaacgag aataggtggc tcgaaaaaac cctgaagcgg gccgagaagt ggaggagtga
     7681 actctcgata atgatccagg ttaaaatact gcatactacc aaatctccgg cggtgggacc
     7741 gaagaagaag cgcaaggtgg ggaccatgac taatctctca gatataatcg agaaggaaac
     7801 aggaaagcaa ctggtcatcc aagaatcgat tttgatgctt cccgaagaag tcgaagaagt
     7861 tataggaaat aagcccgagt ctgacatact ggttcacaca gcgtacgatg aaagtacgga
     7921 cgagaatgtc atgttgctga catcggacgc acctgaatac aagccttggg ctctggtcat
     7981 acaagatagt aacggagaaa ataagattaa aatgcttgaa ggaaggggct ccctgctgac
     8041 atgtggagat gtggaagaga atccaggacc atccaggatg atagagcaag atggttgtac
     8101 ccaagtgctg cggcccctcg gatggcgggg gtacagcgcc atgactggcc ataatagaca
     8161 gtccgctgct ctcatgcctc catgcagcgg atgtcagagg aggggagcta ggttcttcct
     8221 gtctagacct acctgccccg tgccctagtg gcggccgccg acgtccgatc gttcaaacat
     8281 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     8341 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     8401 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     8461 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     8521 ggaattgatc ccccctcgac agcttccgga aagggcgaat tcgcaacttt gtatacaaaa
     8581 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     8641 aaacagacta cataatactg taaaacacaa catatccagt cactatgcca tccagctgat
     8701 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     8761 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctc
//
