LOCUS CJW5968_pKFSS1_2 6736 bp ds-DNA linear 28-JAN-2020 DEFINITION . ACCESSION urn.local...c-6nnrh9q KEYWORDS . SOURCE null ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1..714 /note="colE1 origin or replication" /locus_tag="colE1 origin or replication" /label="colE1 origin or replication" /ApEinfo_label="colE1 origin or replication" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 791..973 /note="IR2" /locus_tag="IR2" /label="IR2" /ApEinfo_label="IR2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1179..1738) /note="ORF3" /locus_tag="ORF3" /label="ORF3" /ApEinfo_label="ORF3" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1791..2341 /note="ORF2" /locus_tag="ORF2" /label="ORF2" /ApEinfo_label="ORF2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2352..3478 /note="ORF1" /locus_tag="ORF1" /label="ORF1" /ApEinfo_label="ORF1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3710..3892 /note="IR2" /locus_tag="IR2(1)" /label="IR2(1)" /ApEinfo_label="IR2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5138..5544 /note="FlgB promoter" /locus_tag="FlgB promoter" /label="FlgB promoter" /ApEinfo_label="FlgB promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5545..6336 /note="PflgB-aadA" /locus_tag="PflgB-aadA" /label="PflgB-aadA" /ApEinfo_label="PflgB-aadA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 4278..4327 /created_by="User" /locus_tag="synthetic promoter" /label="synthetic promoter" /ApEinfo_label="synthetic promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4336..4835 /created_by="User" /locus_tag="random 500 bp" /label="random 500 bp" /ApEinfo_label="random 500 bp" /ApEinfo_fwdcolor="#279ed8" /ApEinfo_revcolor="#279ed8" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" binding_site 4844..4885 /created_by="User" /locus_tag="dCas9 binding region" /label="dCas9 binding region" /ApEinfo_label="dCas9 binding region" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 4886..4926 /created_by="User" /locus_tag="terminator" /label="terminator" /ApEinfo_label="terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 61 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 181 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 661 acgccagcaa cgcggccttt ttacggttcc tgggcttttg ctggcctttt gctcacatgt 721 tctttcctgc actagatccc cggcaagttc atccttaggg tttccatatc tcttaaaaag 781 tcaatttctg cttgattaat ttcttgtgga ttattgtttt tgcggttttt cattttttat 841 ctccttgagt attgaatttt ttcttatata taaatatata gcaaaaacta tttttgccaa 901 cttttttaca aaaaattttt tattaaaaca cttagggctt tgctaaattc tcttttaaaa 961 gaacttagta aagccctatg gatttaagaa ctgattatac ttacgtagta aaaatactac 1021 agatattaat agtatagacc atatcaaaat cataatcaat ttatatttat ctaaaattgt 1081 agtccataca aaatacaaaa aaattaggtt tttctaaatt ctattttaaa agaacttagt 1141 aacacctaat ctctaaatta cttacaaaat tctaaaatta gatttttagt tcttcatatt 1201 tctttaaaag ttttttaaga aaatcttttt gattttcaaa aatttcatgc atcataaaac 1261 tagtaaattt ggattttttt ttgtaaaaat cataactttc ttgggtttta agttgaaatc 1321 ttaatggttt tattggattt tgcctagatt tttttaacgc cggactttct ttatctttta 1381 taaaattata agaattttta ataccatttt ctattaaata atcttctttt ataagcccct 1441 cttgcagtgc agttgctaat cttaaatact tatacgcttg agtttttgct acatcaaagt 1501 cttttataaa atcaagaaaa gttttataac catcaaattt ataatattga ttatctttta 1561 tttcttttaa aattttaata ttttccaccc tgtggaatat ttcttttttg aaattgtcat 1621 ttaacttttc ctttagtgtc ttgtaataag aaattttact ttcattaatc attaaatttt 1681 gttcaacata agtatctatc ttttttgtta aaactttaac aacacgtttt ttttccataa 1741 aaattttcct ttatttttgc aacattttcc ttaattgata taattgttac tattttagat 1801 tttttaatat ccacttaaga attaatccct cactagaata ttttttatta tagaattcat 1861 atacatgttt ttctaaatct ataaatttat caacaattgc tttattatag ggtgtatcta 1921 tcttttcttt tcttaataat cgtctcatat tctttaaata acaacaaaca ctgccggttt 1981 taaacctaaa ttctatgtaa tatatttttg aaaaagtata tacattttca accccgttta 2041 tttcatactt tacaaaaaca ttatttattg gttttctata accatagtaa atgcctaaaa 2101 atttgtcccc gtctttcata ggatatagat aaagtttttg aatctctgat ttatctaata 2161 atttttcaaa aaaaattata caccgctttt gtttgttact aactactcta tgcaatgcct 2221 ttaatatttt tgtgtgatat atatttttat cacctttttt ttctttcagc aggaagatac 2281 tttcttttgg aaatttaatt ttttgttcat ttattcttgc agccttaagt ccgattttca 2341 tatttaatcc ttatatagcc tttctattta attcttttaa actcaataaa cttttttggt 2401 ttttcattag ttctaataat tcgcaataat atgtgcaaaa cactttattg tattctattt 2461 tcttttggtt attcaaatat tcttttataa ttggctttag aagttcaata tttacttctt 2521 tttttagttg ttcaataagg atattgaaaa tgtttgtttt taaatcttga taattttgtt 2581 gagaattttc tttttttctt tcaattgact tctctaattt acgctttata ttgtttaaat 2641 cgctatattt atgactttca ataataaaat gaggcttaaa tttgtaattt tcgtatattt 2701 tttggaaact tgtttctaat tgtttagaat tatattcatt tttttcaaat tctttttgag 2761 tgttgcatag aatttccttt aatttttctt gtttttcttt catacaagat ttagtaaaat 2821 gtatattttt attttttagt aatttaattt ctattatttt tatgatttta agtattttga 2881 ttatattatc tttatcaata tttaaattga ataaaattgt aagaaaaatt gaaagaattt 2941 ttttacataa aaagttacat ttattgaagt aatttcttac ttgatacttt tctatttcat 3001 taatctttct ttcttctttt atattatttt tattatttaa acactccttt aaatctacac 3061 tcccattttt agtaaatcta tctttaaggc cccttgaaac tctattttga aatcttaaat 3121 cttttttttc ttgaaagtat ttatagattt tttggtaaca ttcttttttt gaaaaattaa 3181 gttgatagta tatttcggtc cccaaattta cccctaagtg tttgtgataa ttagttgtga 3241 ctttaaattc tttttctaat ttgtaaaggt aattttgcag tgtttttagt ttaacgggtg 3301 tttgactatt tcttttaagg ttttcattaa agtaatagag tatgtttttt tgtgtgtatt 3361 tcttatatgt tttatttatg tacgctagtg ttgagataag aactactaat ttatactgta 3421 atttattgta gcaagttgtg gttttactgg ttttgtttat attagataag ttttccattt 3481 atagctcctt gaataccgga ttttttatct ataaagtata tatatcacaa ttttgattta 3541 aaagtaaatt ctttttttaa aaatacaaaa attttttata aattatatag accatgatat 3601 tgtattaata ctaattgatt ttaatttaag gttgaactaa aataaacata gttttgtaga 3661 aaataatttt tcattatttt gctacttgat attggatcgt aaaaatatta gggctttaca 3721 aagttctttt aaaagagaat ttagtaaagc cctaagtctt ttaataaaaa attttttgta 3781 aaaaagttgg caaaaatagt ttttgctata tatttatata taagaaaaaa ttcaatactc 3841 aaggagataa aaaatggaaa acttaaacaa taatccacaa aaaattaatc aaataaataa 3901 gcaaactaat tttattggtt ttgaaaattt tatagaactt gataatcaag ttaatcaaac 3961 taaagaaaaa agtaaattaa gtaagatagg taagaaatta cccggaataa gcagtcaagt 4021 ctagtctagt gcggccgcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 4081 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 4141 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 4201 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 4261 attacgaatt cgagctcgaa ttctaaagat ctttgacagc tagctcagtc ctaggtataa 4321 tactagtgga agagccaaag tcaacactta tagacaaata tgatctttct aaccttcacg 4381 aaatagcatc agggggagca cctttgtcta aggaagtagg agaggcagtt gcaaagaggt 4441 ttcatttacc cggaattagg caaggatatg ggttaacaga aaccacatct gcaattttaa 4501 ttacacctga aggagatgat aaaccaggag ctgttgggaa ggttgttcct ttttttgagg 4561 ctaaagttgt tgatttagat actggaaaga ctttaggagt taatcaaaga ggcgagttat 4621 gtgtaagagg acctatgata atgtcaggat acgtaaataa tccagaagct acaaatgcat 4681 tgatagataa agatggttgg ttacactcag gcgatatagc ttattgggac gaagatgaac 4741 acttttttat agttgataga ttgaaatctt tgattaagta taaaggttat caagtagctc 4801 cagcagaatt ggaaagtatt ttattgcaac acccagctct tccgttttag agctagaaat 4861 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct 4921 ttttttgaag cttgggcccg aacaaaaact caaagcttgg cactggccgt cgttttacaa 4981 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 5041 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 5101 agcctgaatg gcgaatggcg cggccgccct aggttaatac ccgagcttca aggaagattt 5161 cctattaagg ttgaacttaa gagcttaagc atagacgatt tgaaaaaaat tttaaaacaa 5221 acaaaaaatt ctttaataaa acaatatgtt gcgatgttta aggtttatga tttagattta 5281 aagtttagtg aggaagctat agatagaatt gcagagctta cttttaatat gaatcttgag 5341 agtgaaaatc ttggtgccag aagacttcac ggtgttatgg aaatagtgct tgcagatctt 5401 ttttttgaag tgcctggcag taagttgaaa aaatttgaaa taaacttgga ctatgttaat 5461 aaaaaaatac aaattaacga acaaaaagat ttgaattatt atataattta gttaaaagca 5521 attttaaatg agggaggttt ccatatgagg gaagcggtga tcgccgaagt atcgactcaa 5581 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat 5641 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt 5701 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa 5761 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg 5821 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg 5881 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct 5941 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa 6001 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta 6061 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc 6121 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca 6181 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt 6241 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac 6301 gtgaaaggcg agatcaccaa ggtagtcggc aaataagacg tcgccggagc ggtcgagttc 6361 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc 6421 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc 6481 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg 6541 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg 6601 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag 6661 caggactgac acgtgctaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 6721 tttgataatc tcatga //