LOCUS CJW7165_pBbdCas9 11320 bp ds-DNA circular 12-APR-2020 DEFINITION Inducible luciferase construct for Bb. ACCESSION urn.local...f-82pymqq VERSION urn.local...f-82pymqq KEYWORDS . SOURCE ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS complement(4036..5118) /created_by="User" /modified_by="User" /locus_tag="synthetic lacI" /label="synthetic lacI" /ApEinfo_label="synthetic lacI" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="#f8c6f9" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 9752..10543 /created_by="User" /modified_by="User" /locus_tag="aadA" /label="aadA" /ApEinfo_label="aadA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="#f8c6f9" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5476..9582 /locus_tag="dCas9" /label="dCas9" /ApEinfo_label="dCas9" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="#f8c6f9" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator complement(3944..4000) /locus_tag="Terminator" /label="Terminator" /ApEinfo_label="Terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 10596..11309 /locus_tag="ColE1 Ori" /label="ColE1 Ori" /ApEinfo_label="ColE1 Ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 12..3294 /locus_tag="bb-ORF123-IRS" /label="bb-ORF123-IRS" /ApEinfo_label="bb-ORF123-IRS" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3480..3929 /note="rifampicin ADP-ribosylating transferase (Klebsiella pneumoniae) ADI49225.1" /locus_tag="rifampicin ADP-ribosylating transferase arr2" /label="rifampicin ADP-ribosylating transferase arr2" /ApEinfo_label="rifampicin ADP-ribosylating transferase arr2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 9605..9751 /locus_tag="PflgB " /label="PflgB " /ApEinfo_label="PflgB " /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat 61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct 121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt 181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact 241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata 301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc 361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac 421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt 481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta 541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat 601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa 661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc 721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt 781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct 841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac 901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca 961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt 1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt 1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca 1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt 1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac 1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca 1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg 1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt 1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat 1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct 1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt 1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc 1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt 1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt 1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa 1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta 1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg 1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg 2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata 2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata 2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta 2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc 2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca 2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt 2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga 2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta 2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga 2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta 2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta 2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc 2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt 2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat 2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata 2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt 3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa 3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga 3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa 3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag 3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt 3301 ctagtgcggc cgcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 3361 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 3421 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgagga ggtaacgtca 3481 tggtaaaaga ttggattcct atttctcatg ataattataa acaagtacaa ggaccatttt 3541 atcatggaac taaagctaat ttagcaattg gtgatttact aactacagga tttatttctc 3601 attttgaaga tggtcgaatt cttaaacata tttatttttc agctttaatg gaaccagcag 3661 tttggggagc tgaacttgct atgtcactat ctggtcttga aggtcgtggt tatatatata 3721 tagttgaacc aacaggacca tttgaagatg atccaaatct tacaaataaa aaatttcctg 3781 gtaatccaac acaatcttat agaacttgtg aacctttaag aattgttggt gttgttgaag 3841 attgggaagg acatcctgtt gaattaataa gaggaatgtt agattcatta gaagatttaa 3901 aacgtcgtgg tttacatgtt attgaagatt aaCCTggcgc gccaaataat gcccgtgtcc 3961 ctgttgaata gggacacggt tttttaacaa tttttttatt cctaggcctt agtggccggt 4021 acccggggat ccttattact ggccgctttc tagcctggat acttgtctgg ctagttgcat 4081 tagagagtca gctagagccc tagggctagc tgtttgtgta ttcggagcca gtgtagtttt 4141 tctttttact aacgatacgg gtaataattg attgcctttt acagcctgac cttgagacaa 4201 ttgcaaaagt ctgtccacag aggtttgacc taataatcta aaatcttgtt tgatagtagt 4261 aagtggaggt atgtagcaac tagaatcttc tgtatcatca taacccacaa ctgaaatatc 4321 agccccaact ctaagtccac tttcagtaat agctcgcata gctcctaaag ccatttgatc 4381 attagcaact aacatagcag tagggacaat gccttcattt aacatttgca ttgtttgctg 4441 aaatcctgac atagcagacc aatctccttc tctttctgct attggttgta tttgatttct 4501 tgttaaatac ttgtgccatc ctgctaatct caatctggca cttactgaac ttaagggacc 4561 tgcaagTagT gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt 4621 tccatcttcg tgagaaaaaa ttatagagtt tattggggtt tggtccgaca catccaaaaa 4681 caatgctgga acattagtac atgctgcttc aactgcaatc gcatcttggt catcaagagg 4741 ataattaatt ataagtccac taacacgttg agctaataaa ttatgtactg ctgccttaca 4801 agcctctaca cctgatctct caaccattga tacaacaacg cttgcaccaa gttgatcagc 4861 tctcgattta attgcggcta caatttgact aggcgcatgt aatgcaagtg atgatgtggc 4921 aactccaatt aaaagtgatt gtttccccgc aagttgctga gcaacgcgat ttggtatata 4981 attcaactct gccatagctg cttcaacctt ctctcttgtt tttgcagata catgacttgc 5041 ttggttcaca actctcgaaa ccgtttggta gctaaccccg gcatattcag caacatcata 5101 caaagtaaca ggtttcatgg atcattcctc catgataaaa tttaaatttc tgactttggc 5161 aaatcctttt gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa acgcaaatag 5221 caatagcatt aaaaataatt attattattt atttctttga ttaaaatcaa aagcctttaa 5281 tgtattttct tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc 5341 cacttttctc tctctatctt ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg 5401 ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa 5461 gaAgagaaat tacatatgga taagaaatac tcaataggct tagctatcgg cacaaatagc 5521 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg 5581 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt 5641 ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg 5701 aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat 5761 agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt 5821 catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc 5881 tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat 5941 ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat 6001 cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta 6061 tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga 6121 ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat 6181 ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat 6241 tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta 6301 gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat 6361 ttatcagatg ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct 6421 cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta 6481 aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca 6541 aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt 6601 atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt 6661 gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac 6721 ttgggtgagc tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac 6781 aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg 6841 gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca 6901 tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg 6961 acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat 7021 gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga 7081 aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca 7141 aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt 7201 gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat 7261 gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc 7321 ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga 7381 cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt 7441 tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct 7501 ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag 7561 ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga 7621 caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa 7681 ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag 7741 ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa 7801 aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt 7861 cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat 7921 ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat 7981 tatgatgtcg atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag 8041 gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta 8101 gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt 8161 aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt 8221 tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg 8281 gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg 8341 attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta 8401 cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact 8461 gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt 8521 tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa 8581 tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga 8641 gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat 8701 aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc 8761 aagaaaacag aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat 8821 tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat 8881 agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag 8941 aagttaaaat ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa 9001 aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc 9061 attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct 9121 agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt 9181 ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa 9241 caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa 9301 ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac 9361 aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg 9421 acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga 9481 tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt 9541 tatgaaacac gcattgattt gagtcagcta ggaggtgact aataaaagct tgatcagatc 9601 tcagcttttt tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa acttggacta 9661 tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg aattattata taatttagtt 9721 aaaagcaatt ttaaatgagg gaggtttcca tatgagggaa gcggtgatcg ccgaagtatc 9781 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc 9841 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt 9901 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct 9961 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat 10021 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg 10081 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga 10141 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc 10201 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt 10261 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt 10321 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga 10381 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc 10441 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt 10501 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgacgtc gccggagcgg 10561 tcgagttctg gaccgaccgg ctcgggttct catgaccaaa atcccttaac gtgagttttc 10621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 10681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 10741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 10801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 10861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 10921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 10981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 11041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 11101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 11161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 11221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 11281 gttcctgggc ttttgctggc cttttgctca catgttcttt //