LOCUS       pTA-Mob_2.0            56450 bp    DNA     circular UNA 26-APR-2020
DEFINITION  pTA-MOB derivative with yeast artifical chromosome elements & an
            origin of transfer (oriT). Developed by the Karas laboratory. (DOI:
            https://doi.org/10.3390/ijms20205212).
ACCESSION   urn.local...3s-beu8im7
VERSION     urn.local...3s-beu8im7
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli.
FEATURES             Location/Qualifiers
     rep_origin      1..101
                     /note="annotated based on pTA-Mob (Strand et al., 2014);
                     NB position not confirmed!"
                     /function="pBBR1 origin of replication"
                     /label="ori"
     CDS             395..1057
                     /function="required for pBBR1 plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep replication protein"
                     /protein_id="AAB06694.1"
                     /Transferred_Translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAG
                     LIARKPTAAMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISV
                     VKLNGPGTVSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPT
                     LYPGEQQLPTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDG
                     EPLEPPTRVTLPRR"
                     /Transferred_From="XXU25061"
                     /Transferred_Similarity="100.00%"
                     /label="rep"
     gene            395..1057
                     /gene="rep"
                     /Transferred_From="JX514367"
                     /Transferred_Similarity="100.00%"
     misc_feature    1492..3917
                     /note="inserted region to make pTA-Mob 2.0, containing
                     yeast elements and RP4 oirigin of transfer (oriT)"
                     /SECDrawAs="Region"
                     /label="HIS3-CEN6-ARSH4-oriT"
     regulatory      1620..1781
                     /regulatory_class="promoter"
                     /note="HIS3 promoter from pmycYACTn"
                     /Transferred_From="GU593054"
                     /Transferred_Similarity="99.38%"
                     /gene="HIS3"
                     /label="HIS3 promoter"
     CDS             1808..2470
                     /gene="HIS3"
                     /codon_start=1
                     /transl_table=1
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="ADE19327.1"
                     /Transferred_From="GU593054"
                     /Transferred_Similarity="99.25%"
                     /note="HIS3 from pmycYACTn"
                     /function="histidine biosynthesis, selectable marker for
                     histidine-auxotrophic S. cerevisiae"
                     /label="HIS3"
     Centromere      2631..2747
                     /function="S. cerevisiae centromere from genomic location
                     II-255"
                     /note="CEN6 from pmycYACTn"
                     /Transferred_From="GU593054"
                     /Transferred_Similarity="100.00%"
                     /db_xref="OriDB:34"
                     /label="CEN6"
     misc_feature    <3147..3391
                     /note="complete incP origin of transfer (from RP4)"
                     /label="oriT"
                     /note="Geneious type: Origin of transfer"
     misc_feature    3147..3917
                     /note="inserted origin of transfer region from RP4;
                     includes duplication of traJ, traX, and part of traI"
                     /SECDrawAs="Region"
                     /label="oriT region"
     operon          complement(<3147..3206)
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="96.67%"
                     /label="traKLM operon"
     regulatory      complement(3178..3183)
                     /regulatory_class="minus_10_signal"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traKLM -10"
     protein_bind    complement(3192..3205)
                     /operon="traKLM"
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbA binding site"
     operon          3195..>3917
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="60.35%"
                     /label="traJXIH operon"
     regulatory      3195..3200
                     /regulatory_class="minus_35_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="promoter 1 of TraJXIH operon"
                     /label="traJXIH P1 -35"
     regulatory      complement(3201..3206)
                     /regulatory_class="minus_35_signal"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traKLM -35"
     protein_bind    complement(3215..3228)
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbA binding site"
     regulatory      3218..3223
                     /regulatory_class="minus_10_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="promoter 1 of TraJXIH operon"
                     /label="TraJXIH P1 -10"
     regulatory      3225..3230
                     /regulatory_class="minus_35_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="promoter 2 of TraJXIH operon"
                     /label="traJXIH P2 -35"
     regulatory      3248..3253
                     /regulatory_class="minus_10_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="Promoter 2 of TraJXIH operon"
                     /label="traJXIH P2 -10"
     misc_feature    3280..3391
                     /note="minimal incP origin of transfer (from RP4);
                     diminished transfer activity when only minimal region is
                     present"
                     /label="Minimal oriT"
                     /note="Geneious type: Origin of transfer"
     misc_feature    complement(3284..3285)
                     /note="oriT nick site between these 2 bases"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="oriT nick site"
     protein_bind    complement(3285..3290)
                     /bound_moiety="TraI"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="TraI binding site"
     repeat_region   complement(3293..3332)
                     /rpt_type="INVERTED"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    complement(3296..3308)
                     /bound_moiety="TraJ"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TraJ binding site"
     CDS             3424..3795
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.73%"
                     /note="duplicated when cloning in oriT to make pTA-Mob
                     2.0"
                     /label="traJ"
     gene            3424..3795
                     /gene="traJ"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     3727..3746
                     /Sequence="ATCGAAGAGAAGCAGGACGA"
                     /Hairpin_Tm="43.6"
                     /Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6374
                     /label="Fragment_2_F"
     misc_feature    3727..10100
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 2"
     CDS             3792..3833
                     /note="duplicated when cloning in oriT to make pTA-Mob
                     2.0"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traX"
     gene            3792..3833
                     /gene="traX"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    3800..3812
                     /note="OB4"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="duplicated when cloning in oriT to make pTA-Mob
                     2.0"
                     /label="Operator B4"
     CDS             3830..>3917
                     /gene="traI"
                     /operon="traJXIH"
                     /codon_start=1
                     /transl_table=11
                     /product="TraI DNA relaxase"
                     /protein_id="CAJ85725.1"
                     /db_xref="InterPro:IPR005094; UniProtKB/TrEMBL:A6H977"
                     /Transferred_Translation="MIAKHVPMRSIKKSDFAELVKYITDEQGKTER
                     LGHVRVTNCEANTLPAVMAEVMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIE
                     DRICAGLGFAEHQRVSAVHHDTDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATL
                     ERDYGLERDNHETRQRVSENRANDMERHAGVESLVGWIKRECLPELQAAQSWEDLHRV
                     LRENGLKLRERGNGFIFEAGDGTTVKASTVSRDLSKPKLEARFGAFTPAEGGEAPRRR
                     EYRAKPLKTRIDTTELYARYQSERQEMGAVRKGELDTLRRRRDRLIEAAMRSNRLRRA
                     AIKLLGEGRIAKRLMYAQAHKALRADLDKINREYRQGRQAVQERTQRRAWADWLKAEA
                     MKGDDKALAALRAREGRSDLKGNTIQGSGEAKPGHAAVTDNITKKGTIIYRVGSSAVR
                     DDGDRLQVSREATTDGLDAALRLAMERFGDRITVNGTAEFKERIAQAAAAGRLAITFD
                     DAALERRRQELLTKEQAHEQPERNDGRRDRGGDGGIRPAAARTTLNATGGDGDRRDAR
                     AVSAGGTVALRKPNVGRIGRKPPPQSQNRLRALSQLGVVRIAGGAEMLLPRDVPGHVE
                     QQGAEPAHALRRGVSGPGRGLKPEQIAAAEKYVAEREQKRLNGFDIPKHARYTDYVGA
                     LSYAGTRNVEDQALALLRKENDEILVLPVDKATVQRMKRLAIGDPVTVTPRGSLKTTR
                     GRSR"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="15.23%"
                     /note="duplicated when cloning in oriT to make pTA-Mob
                     2.0"
                     /label="traI"
     gene            3830..>3917
                     /gene="traI"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="15.23%"
     primer_bind     complement(4084..4123)
                     /Sequence="TTTAACCTACTTCCTTTGGTTCCGGGGGATCTCGCGACTC"
                     /Hairpin_Tm="38.1"
                     /Tm="67.9"
                     /Self_Dimer_Tm="26.8"
                     /%GC="52.5"
                     /Pair_Dimer_Tm="7.5"
                     /Product_Size=5791
                     /label="Fragment_1_R"
     operon          complement(<4491..6740)
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.91%"
                     /label="ssb,trfA,upf16.5 operon"
     CDS             complement(4491..5158)
                     /gene="upf16.5"
                     /operon="ssb,trfA,upf16.5"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAJ85683.1"
                     /db_xref="GOA:A6H935; InterPro:IPR000182;
                     InterPro:IPR001647; InterPro:IPR012287;
                     InterPro:IPR016181; UniProtKB/TrEMBL:A6H935"
                     /Transferred_Translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKR
                     ALLDALASLSIARAHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKK
                     APEMFGSVYEHEEKKPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHL
                     SGVFVEPEQRGRGVAGMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSL
                     LFISVYRLPGGSRRR"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.70%"
                     /label="upf16.5"
     gene            complement(4491..5158)
                     /gene="upf16.5"
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.70%"
     CDS             complement(5133..6281)
                     /gene="trfA"
                     /operon="ssb,trfA,upf16.5"
                     /function="initiation of vegetative DNA replication"
                     /note="essential for replication from oriV in P.
                     aeruginosa; long form TrfA1, alternative start codon; oriV
                     was replaced with pBBR1 ori when generating pTA-Mob"
                     /codon_start=1
                     /transl_table=11
                     /product="TrfA replication protein, long form"
                     /protein_id="CAJ85684.1"
                     /db_xref="InterPro:IPR010751; UniProtKB/TrEMBL:A6H936"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trfA1"
     CDS             complement(5133..5990)
                     /gene="trfA"
                     /operon="ssb,trfA,upf16.5"
                     /function="initiation of vegetative DNA replication"
                     /note="sufficient (with oriV) for replication in all
                     hoststested except P. aeruginosa; short form TrfA2,
                     alternativestart codon; oriV was replaced with pBBR1 ori
                     whengenerating pTA-Mob"
                     /codon_start=1
                     /transl_table=11
                     /product="TrfA replication protein, short form"
                     /protein_id="CAJ85685.1"
                     /db_xref="InterPro:IPR010751; UniProtKB/TrEMBL:A6H937"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trfA2"
     gene            complement(5133..6281)
                     /gene="trfA"
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(6330..6680)
                     /gene="ssb"
                     /operon="ssb,trfA,upf16.5"
                     /codon_start=1
                     /transl_table=11
                     /product="single stranded DNA binding protein"
                     /protein_id="CAJ85686.1"
                     /db_xref="GOA:A6H938; InterPro:IPR000424;
                     InterPro:IPR011344; InterPro:IPR012340;
                     InterPro:IPR016027; UniProtKB/TrEMBL:A6H938"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="high-affinity binding to single-stranded DNA"
                     /label="ssb"
     gene            complement(6330..6680)
                     /gene="ssb"
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    6580..6593
                     /note="regulatory significance unknown"
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      6621..6626
                     /regulatory_class="minus_35_signal"
                     /gene="trbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbA -35"
     gene            order(6621..6626,6644..6649,6801..7166)
                     /gene="trbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      6644..6649
                     /regulatory_class="minus_10_signal"
                     /gene="trbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbA -10"
     regulatory      complement(6711..6716)
                     /regulatory_class="minus_10_signal"
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="ssb,trfA,upf16.5 -10"
     protein_bind    6713..6722
                     /note="OA3"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A3"
     regulatory      complement(6735..6740)
                     /regulatory_class="minus_35_signal"
                     /operon="ssb,trfA,upf16.5"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="ssb,trfA,upf16.5 -35"
     protein_bind    6745..6757
                     /note="OB10"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B10"
     CDS             6801..7166
                     /gene="trbA"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbA repressor protein"
                     /protein_id="CAJ85687.1"
                     /db_xref="GOA:A6H939; InterPro:IPR001387;
                     InterPro:IPR010982; UniProtKB/TrEMBL:A6H939"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="regulation of trb gene expression"
                     /note="helix-turn-helix XRE-family like proteins;
                     prokaryotic DNA binding proteins belonging to the
                     xenobiotic response element family of transcriptional
                     regulators; cl09100"
                     /label="trbA"
     misc_feature    6855..20272
                     /SECDrawAs="Region"
                     /note="region annotated based on pTA-Mob (Strand et al.,
                     2014)"
                     /label="Tra2"
     protein_bind    7196..7208
                     /note="OB9"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B9"
     operon          7356..22147
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.96%"
                     /label="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8 operon"
     regulatory      7356..7361
                     /regulatory_class="minus_35_signal"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trb,upf31.7,fiwA,upf32.8 -35"
     protein_bind    7372..7385
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      7379..7384
                     /regulatory_class="minus_10_signal"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trb,upf31.7,fiwA,upf32.8 -10"
     CDS             7437..8396
                     /gene="trbB"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="ATPase; autophosphorylase; VirB11 homologue;
                     probable general secretion pathway protein E (Type II
                     traffic warden ATPase); Flp pilus assembly protein"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbB protein"
                     /protein_id="CAJ85688.1"
                     /db_xref="GOA:A6H940; InterPro:IPR001482;
                     InterPro:IPR003593; InterPro:IPR014149;
                     UniProtKB/TrEMBL:A6H940"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as klbA, kilBI"
                     /label="trbB"
     gene            7437..8396
                     /gene="trbB"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             8409..8846
                     /gene="trbC"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="Precursor of TrbC pilin subunit; mating pair
                     formation; VirB2 homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC propilin protein"
                     /protein_id="CAJ85689.1"
                     /db_xref="InterPro:IPR007039; UniProtKB/TrEMBL:A6H941"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as klbB"
                     /label="trbC"
     gene            8409..8846
                     /gene="trbC"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             8849..9160
                     /gene="trbD"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbD protein"
                     /protein_id="CAJ85690.1"
                     /db_xref="InterPro:IPR007792; InterPro:IPR016704;
                     UniProtKB/TrEMBL:A6H942"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as klbC"
                     /label="trbD"
     gene            8849..9160
                     /gene="trbD"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             9157..11715
                     /gene="trbE"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation; VirB4 homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbE protein"
                     /protein_id="CAJ85691.1"
                     /db_xref="GOA:A6H943; InterPro:IPR003593;
                     InterPro:IPR004346; InterPro:IPR018145;
                     UniProtKB/TrEMBL:A6H943"
                     /Transferred_Translation="MIQAIAIAIAGLGALLLFILFARIRAVDAELK
                     LKKHRSKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWLYKGDDNASSTDQQREVVSAR
                     INQALAGLGSGWMIHVDAVRRPAPNYAERGLSAFPDRLTAAIEEERRRHFESLGTMYE
                     GYFVLTLTWFPPLLAQRKFVELMFDDDATAPDRKARTRGLIDQFKRDVRSIESRLSSA
                     VSLTRLKGHKIVNEDGTTVTHDDFLRWLQFCVTGLHHPVQLPSNPMYLDALVGGQEMW
                     GGVVPKVGRKFVQVVALEGFPLESYPGILTALGELPCEYRWSSRFIFMDQHEAVKHLD
                     KFRKKWRQKIRGFFDQVFNTNTGPVDQDALSMVADAEAAIAEVNSGIVAVGYYTSVVV
                     LMDEDRTRLEAAARDVEKAVNRLGFAARIESINTLDAFLGSLPGHGVENVRRPLINTM
                     NLADLLPTSTIWTGNANAPCPMYPPLSPALMHCVTQGSTPFRLNLHVRDLGHTFMFGP
                     TGAGKSTHLAILAAQLRRYAGMSIFAFDKGMSMYPLAAGIRAATKGTSGLHFTVAADD
                     ERLAFCPLQFLSTKGDRAWAMEWIDTILALNGVETTPAQRNEIGNAIMSMHASGARTL
                     SEFSVTIQDEAIREAIRQYTVDGAMGHLLDAEEDGLALSDFTVFEIEELMNLGEKFAL
                     PVLLYLFRRIERALTGQPAVIILDEAWLMLGHPAFRAKIREWLKVLRKANCLVLMATQ
                     SLSDAANSGILDVIVESTATKIFLPNIYARDEDTAALYRRMGLNARQIEILAQAVPKR
                     QYYYVSENGRRLYDLALGPLALAFVGASDKESVAIIKNLEAKFGDQWVDEWLRGRGLA
                     LDEYLEAA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.96%"
                     /label="trbE"
     gene            9157..11715
                     /gene="trbE"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.96%"
     primer_bind     9659..9678
                     /Sequence="TCGAGCTGATGTTTGACGAC"
                     /Hairpin_Tm="None"
                     /Tm="58.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6137
                     /label="Fragment_3_F"
     misc_feature    9659..15795
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 3"
     primer_bind     complement(10081..10100)
                     /Sequence="TGCTGGTCCATGAAGATGAA"
                     /Hairpin_Tm="None"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6374
                     /label="Fragment_2_R"
     CDS             11712..12470
                     /gene="trbF"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbF protein"
                     /protein_id="CAJ85692.1"
                     /db_xref="GOA:A6H944; InterPro:IPR007430;
                     UniProtKB/TrEMBL:A6H944"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbF"
     gene            11712..12470
                     /gene="trbF"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             12482..13375
                     /gene="trbG"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation; VirB9 homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG protein"
                     /protein_id="CAJ85693.1"
                     /db_xref="InterPro:IPR010258; InterPro:IPR014142;
                     UniProtKB/TrEMBL:A6H945"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbG"
     gene            12482..13375
                     /gene="trbG"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             13379..13861
                     /gene="trbH"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbH protein"
                     /protein_id="CAJ85694.1"
                     /db_xref="InterPro:IPR010837; UniProtKB/TrEMBL:A6H946"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbH"
     gene            13379..13861
                     /gene="trbH"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             13866..15257
                     /gene="trbI"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation; VirB10 homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI protein"
                     /protein_id="CAJ85695.1"
                     /db_xref="InterPro:IPR005498; UniProtKB/TrEMBL:A6H947"
                     /Transferred_Translation="MSEDQMAPDASPDAVKPKSGVRRVNNMPMYLI
                     GGVLGIFLLVMALVAADRAAQQNQPGAAKAEKAGSTSMFADEIAGKQQDGIIKAKPLE
                     IPPEQTAQQPTTELTPAPAQGTTITVARPENLDQPPTPPQGARNEDLDRIRMAKLQML
                     EEAIKAKTTVRIDAPRSQGSAGGGAPQGREETLARIQELRRQAENARATDPTAAYQAA
                     LAQARTMGGAAGGGGMGGSGAPTLVQTSNRSGGGAGYGSFDNRSEGDRWRLDSQPEAP
                     ATPYVLRAGFVVPATLISGINSDLPGQIMAQVSQSVYDTATGKHMLIPQGSRLVGSYS
                     NDVAYGQKRVLVAWQRIIFPDGKAMDIGAMPGGDSAGYAGFNDKVNNHYFRTFASAFL
                     MSGVVAGISLSQDRGNSNSGYGRQDAGSAMSEALGQQLGQVTAQMIAKNLNIAPTLEI
                     RPGYRFNVIVTKDMTFSKPYQAFDY"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.93%"
                     /label="trbI"
     gene            13866..15257
                     /gene="trbI"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.93%"
     CDS             15274..16050
                     /gene="trbJ"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation; entry exclusion"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbJ protein"
                     /protein_id="CAJ85696.1"
                     /db_xref="InterPro:IPR014147; UniProtKB/TrEMBL:A6H948"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbJ"
     gene            15274..16050
                     /gene="trbJ"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    15297..15309
                     /gene="trbJ"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /note="OB8"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B8"
     primer_bind     15525..15564
                     /Sequence="GGACCAGGCGCAGTCCACCATCAACGGCCTGATGAGCGCC"
                     /Hairpin_Tm="47.9"
                     /Tm="75.0"
                     /Self_Dimer_Tm="32.9"
                     /%GC="67.5"
                     /Pair_Dimer_Tm="16.5"
                     /Product_Size=6000
                     /label="Fragment_4_F"
     misc_feature    15525..21524
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 4"
     regulatory      complement(15736..15768)
                     /regulatory_class="terminator"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Terminator"
     primer_bind     complement(15776..15795)
                     /Sequence="GGACTTGAGGTTGCTCTGCT"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6137
                     /label="Fragment_3_R"
     CDS             16062..16271
                     /gene="trbK"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbK lipoprotein"
                     /protein_id="CAJ85697.1"
                     /db_xref="UniProtKB/TrEMBL:A6H949"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="entry exclusion; involved in surface exclusion"
                     /label="trbK"
     gene            16062..16271
                     /gene="trbK"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             16278..17864
                     /gene="trbL"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation; putative topoisomerase;
                     VirB6 homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbL protein"
                     /protein_id="CAJ85698.1"
                     /db_xref="GOA:A6H950; InterPro:IPR007688;
                     InterPro:IPR014150; UniProtKB/TrEMBL:A6H950"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbL"
     gene            16278..17864
                     /gene="trbL"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             17888..18487
                     /gene="trbM"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM protein"
                     /protein_id="CAJ85699.1"
                     /db_xref="InterPro:IPR009989; UniProtKB/TrEMBL:A6H951"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbM"
     gene            17888..18487
                     /gene="trbM"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             18503..19207
                     /gene="trbN"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbN protein"
                     /protein_id="CAJ85700.1"
                     /db_xref="InterPro:IPR008258; UniProtKB/TrEMBL:A6H952"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbN"
     gene            18503..19207
                     /gene="trbN"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    19221..19233
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /note="OB7"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B7"
     CDS             19238..19501
                     /gene="trbO"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbO protein"
                     /protein_id="CAJ85701.1"
                     /db_xref="UniProtKB/TrEMBL:A6H953"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="conjugal transfer protein"
                     /label="trbO"
     gene            19238..19501
                     /gene="trbO"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             19538..20272
                     /gene="trbP"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="possible role in pillus assembly; putatively
                     involved in mating pair formation"
                     /codon_start=1
                     /transl_table=11
                     /product="TrbP protein"
                     /protein_id="CAJ85702.1"
                     /db_xref="InterPro:IPR008875; UniProtKB/TrEMBL:A6H954"
                     /Transferred_Translation="MNTRAMNDASGRASLPAMVIADGTIEALKWLA
                     LLPMTGDHVNKYLFNGTLPYLFEAGRLALPLFVFVLAYNLARPGALERGLYGRAMKRL
                     LAFGLVASVPFIALGGVVGGWWPLNVMFTLLAATAMLYLVERGRSVAPIALFVVAGGL
                     VEFCWPALLLAASVWLYLKRPTWAAALMALLSCASLWYINGNLWALAVVPLVIVAAGV
                     DLRVPRLRWAFYTYYPLHLAALWLIRIPMREAGYLFFT"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.86%"
                     /label="trbP"
     gene            19538..20272
                     /gene="trbP"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.86%"
     regulatory      19700..19728
                     /regulatory_class="terminator"
                     /gene="trbP"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trbP Terminator"
     CDS             20289..20708
                     /gene="upf31.7"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAJ85703.1"
                     /db_xref="UniProtKB/TrEMBL:A6H955"
                     /Transferred_Translation="MQLLKKCTIAALPLLALSGCALLNIPMPTPPG
                     STPPEMLTVPVAQICRDADKNPVRATELYGKKGLSATGKVQVISEGFKPRYRVLLPPG
                     SASVHAGTDNQLAIKSVSTGQTTRVTGTVKDVSYDHNGCSISLDDAKFY"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.29%"
                     /function="major outer membrane lipoprotein"
                     /label="upf31.7"
     gene            20289..20708
                     /gene="upf31.7"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.29%"
     CDS             20723..21418
                     /gene="fiwA"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /function="fertility inhibition of IncW plasmids"
                     /codon_start=1
                     /transl_table=11
                     /product="FiwA"
                     /protein_id="CAJ85704.1"
                     /db_xref="UniProtKB/TrEMBL:A6H956"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="fiwA"
     gene            20723..21418
                     /gene="fiwA"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     21128..21147
                     /Sequence="GTGGACATTGGTTTCAGCAA"
                     /Hairpin_Tm="40.1"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6289
                     /label="Fragment_5_F"
     misc_feature    21128..27416
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 5"
     CDS             21454..22116
                     /gene="upf32.8"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAJ85705.1"
                     /db_xref="UniProtKB/TrEMBL:A6H957"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="upf32.8"
     gene            21454..22116
                     /gene="upf32.8"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     complement(21500..21524)
                     /Sequence="ATCGGCGTGAAGCCCAACAGGGCCA"
                     /Hairpin_Tm="59.9"
                     /Tm="66.5"
                     /Self_Dimer_Tm="17.7"
                     /%GC="64.0"
                     /Pair_Dimer_Tm="16.5"
                     /Product_Size=6000
                     /label="Fragment_4_R"
     operon          complement(22118..23620)
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parCBA operon"
     regulatory      22118..22147
                     /regulatory_class="terminator"
                     /operon="trbBCDEFGHIJKLMNOP,upf31.7,fiwA,upf32.8"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="trb,upf31.7,fiwA,upf32.8 Terminator"
     regulatory      complement(22118..22147)
                     /regulatory_class="terminator"
                     /operon="parCBA"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parCBA Terminator"
     misc_feature    22140..24293
                     /SECDrawAs="Region"
                     /note="region annotated based on pTA-Mob (Strand et al.,
                     2014)"
                     /label="parABCDE"
     CDS             complement(22140..22799)
                     /gene="parA"
                     /operon="parCBA"
                     /function="partitioning; site-specific recombination;
                     autoregulation"
                     /note="long form ParA1, alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA resolvase"
                     /protein_id="CAJ85706.1"
                     /db_xref="GOA:A6H958; InterPro:IPR006118;
                     InterPro:IPR006119; InterPro:IPR006120;
                     InterPro:IPR012287; UniProtKB/TrEMBL:A6H958"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parA1"
     CDS             complement(22140..22763)
                     /gene="parA"
                     /operon="parCBA"
                     /function="partitioning; site-specific recombination;
                     autoregulation"
                     /note="short form ParA2, alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA resolvase"
                     /protein_id="CAJ85707.1"
                     /db_xref="GOA:A6H959; InterPro:IPR006118;
                     InterPro:IPR006119; InterPro:IPR006120;
                     InterPro:IPR012287; UniProtKB/TrEMBL:A6H959"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parA2"
     gene            complement(22140..22799)
                     /gene="parA"
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(22760..23293)
                     /gene="parB"
                     /operon="parCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB nuclease"
                     /protein_id="CAJ85708.1"
                     /db_xref="GOA:A6H960; InterPro:IPR002071;
                     InterPro:IPR006021; InterPro:IPR016071;
                     UniProtKB/TrEMBL:A6H960"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="rB nuclease; plasmid stabilization;
                     partitioning"
                     /label="parB"
     gene            complement(22760..23293)
                     /gene="parB"
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(23290..23583)
                     /gene="parC"
                     /operon="parCBA"
                     /transl_table=11
                     /product="ParC protein"
                     /protein_id="CAJ85709.1"
                     /db_xref="UniProtKB/TrEMBL:A6H961"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="plasmid stabilization"
                     /label="parC"
     gene            complement(23290..23583)
                     /gene="parC"
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    23566..23586
                     /bound_moiety="ParA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="ParA binding site"
     regulatory      complement(23592..23597)
                     /regulatory_class="minus_10_signal"
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parCBA -10"
     regulatory      complement(23615..23620)
                     /regulatory_class="minus_35_signal"
                     /operon="parCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parCBA -35"
     protein_bind    23631..23649
                     /bound_moiety="ParD"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="ParD binding site"
     operon          23676..>28437
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="72.31%"
                     /label="parDE,upf35.8 operon"
     regulatory      23676..23681
                     /regulatory_class="minus_35_signal"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parDE,upf35.8 -35"
     regulatory      23699..23704
                     /regulatory_class="minus_10_signal"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parDE,upf35.8 -10"
     CDS             23734..23985
                     /gene="parD"
                     /operon="parDE,upf35.8"
                     /function="antidote to ParE; autoregulation"
                     /note="ParDE is a plasmid addiction system"
                     /codon_start=1
                     /transl_table=11
                     /product="ParD antitoxin protein"
                     /protein_id="CAJ85710.1"
                     /db_xref="InterPro:IPR018985; UniProtKB/TrEMBL:A6H962"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parD"
     gene            23734..23985
                     /gene="parD"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             23982..24293
                     /gene="parE"
                     /operon="parDE,upf35.8"
                     /function="growth arrest of plasmid-free segregants; DNA
                     gyrase inhibitor"
                     /note="ParDE is a plasmid addiction system"
                     /codon_start=1
                     /transl_table=11
                     /product="ParE toxin"
                     /protein_id="CAJ85711.1"
                     /db_xref="InterPro:IPR007712; UniProtKB/TrEMBL:A6H963"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="parE"
     gene            23982..24293
                     /gene="parE"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    24295..24307
                     /operon="parDE,upf35.8"
                     /note="OB6; base change from consensus"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B6"
     misc_feature    join(24371..>24820,<28268..28437)
                     /gene="upf35.8"
                     /operon="parDE,upf35.8"
                     /note="probable truncation of theoretical product due to
                     sequence errors; disrupted by insertion of IS21"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="PSEUDO:CAJ85712.1"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.04%"
                     /product="hypothetical protein"
                     /label="upf35.8"
                     /note="Geneious type: truncated protein"
     gene            order(24371..24820,28268..28437)
                     /gene="upf35.8"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.04%"
     repeat_region   24817..24820
                     /gene="upf35.8"
                     /rpt_type="DIRECT"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /mobile_element_type="insertion sequence:IS21"
     mobile_element  join(24821..>25332,<26645..28263)
                     /mobile_element_type="insertion sequence:IS21"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="61.86%"
                     /label="IS8 (IS21)"
     repeat_region   24821..24831
                     /note="left terminal repeat"
                     /rpt_type="INVERTED; TERMINAL"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /mobile_element_type="insertion sequence:IS21"
     misc_feature    join(24922..>25332,<26645..27406)
                     /gene="istA"
                     /operon="parDE,upf35.8"
                     /note="disrupted by insertion of URA3 region; long form
                     istA1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 transposase"
                     /protein_id="PSEUDO:CAJ85713.1"
                     /db_xref="GOA:A6H965; InterPro:IPR001584;
                     InterPro:IPR012337; InterPro:IPR017894;
                     UniProtKB/TrEMBL:A6H965"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="47.16%"
                     /function="integrase"
                     /label="istA1"
                     /note="Geneious type: truncated protein"
     gene            order(24922..25332,26645..27406)
                     /gene="istA"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="47.16%"
     misc_feature    join(24946..>25332,<26645..27406)
                     /gene="istA"
                     /operon="parDE,upf35.8"
                     /note="disrupted by insertion of URA3 region; short form
                     istA2"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 transposase"
                     /protein_id="PSEUDO:CAJ85714.1"
                     /db_xref="GOA:A6H966; InterPro:IPR001584;
                     InterPro:IPR012337; InterPro:IPR017894;
                     UniProtKB/TrEMBL:A6H966"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="46.65%"
                     /function="integrase"
                     /label="istA2"
                     /note="Geneious type: truncated protein"
     misc_feature    25333..26644
                     /SECDrawAs="Region"
                     /note="inserted region from pDMI-1.0 to make pTA-Mob 2.0,
                     containing yeast URA3 marker"
                     /label="URA3"
     regulatory      25339..25629
                     /Transferred_From="pDMI-1.0"
                     /Transferred_Similarity="94.14%"
                     /regulatory_class="promoter"
                     /note="URA3 promoter from pDMI-1.0"
                     /gene="URA3"
                     /label="URA3 promoter"
     gene            25339..26638
                     /gene="URA3"
                     /Transferred_From="pDMI-1.0"
                     /Transferred_Similarity="99.88"
     CDS             25630..26433
                     /Transferred_From="pDMI-1.0"
                     /Transferred_Similarity="99.88%"
                     /transl_table=1
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5'-phosphate (OMP) decarboxylase"
                     /note="URA3 from pDMI-1.0"
                     /db_xref="GeneID:856692; SGD:S000000747"
                     /function="catalyzes the sixth enzymatic step in the de
                     novo biosynthesis of pyrimidines, converting OMP into
                     uridine monophosphate (UMP); converts 5-FOA into
                     5-fluorouracil, a toxic compound"
                     /label="URA3"
     regulatory      26434..26638
                     /Transferred_From="pDMI-1.0"
                     /Transferred_Similarity="86.54%"
                     /regulatory_class="terminator"
                     /gene="URA3"
                     /note="URA3 terminator from pDMI-1.0"
                     /label="URA3 terminator"
     primer_bind     27005..27024
                     /Sequence="GAGCAATGGATAGCCGATGT"
                     /Hairpin_Tm="36.8"
                     /Tm="57.8"
                     /Self_Dimer_Tm="4.1"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6204
                     /label="Fragment_6_F"
     misc_feature    27005..33208
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 6"
     primer_bind     complement(27397..27416)
                     /Sequence="AGCTCATGCATCACAACAGC"
                     /Hairpin_Tm="42.7"
                     /Tm="59.2"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6289
                     /label="Fragment_5_R"
     CDS             27406..28203
                     /gene="istB"
                     /operon="parDE,upf35.8"
                     /codon_start=1
                     /transl_table=11
                     /product="IstB transposition protein"
                     /protein_id="CAJ85715.1"
                     /db_xref="GOA:A6H967; InterPro:IPR001270;
                     InterPro:IPR002611; InterPro:IPR003593;
                     UniProtKB/TrEMBL:A6H967"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="putative ATP-binding protein"
                     /label="istB"
     gene            27406..28203
                     /gene="istB"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     repeat_region   28253..28263
                     /note="right terminal repeat"
                     /rpt_type="INVERTED; TERMINAL"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /mobile_element_type="insertion sequence:IS21"
     regulatory      28258..28263
                     /regulatory_class="minus_35_signal"
                     /gene="aphA"
                     /operon="parDE,upf35.8"
                     /note="formed fortuitous promoter for aphA with this
                     insertion; part of a strong istAB promoter when IS21 is in
                     excised, circular form"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="aphA -35"
     gene            order(28258..28263,28281..28286,28680..>28775,
                     <28782..28910,28959..28987)
                     /gene="aphA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="33.37%"
     repeat_region   28264..28267
                     /rpt_type="DIRECT"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /mobile_element_type="insertion sequence:IS21"
     regulatory      28281..28286
                     /regulatory_class="minus_10_signal"
                     /gene="aphA"
                     /operon="parDE,upf35.8"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="aphA -10"
     misc_feature    join(28680..>28775,<28782..28910)
                     /gene="aphA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phospho-transferase"
                     /protein_id="CAJ85716.1"
                     /db_xref="GOA:A6H968; InterPro:IPR002575;
                     InterPro:IPR011009; UniProtKB/TrEMBL:A6H968"
                     /Transferred_Translation="MPESMAAHVMGYKWARDKVGQSGCAVYRLHSK
                     SGGSDLFLKHGKDAFADDVTDEMVRLRWLAGHISVPSVVSFVRTPNQAWLLTTAIHGK
                     TAYQVLKSDFGARLVVVDALAAFMRRLHAIPVSECSVQQWTTHAGLPERGSIEAGVVD
                     VDDFDKEREGWTAEQVWEAMHRLLPLAPDPVVTHGDFSLDNLLIVEGKVVGCIDVGRA
                     GIADRYQDLAVLWNCLEEFEPSLQERLVAQYGIADPDRRKLQFHLLLDELF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="29.84%"
                     /note="Neomycin-kanamycin phosphotransferase type VIII"
                     /label="aphA"
                     /note="Geneious type: truncated protein"
     operon          complement(28959..38103)
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traGFEDCBA operon"
     regulatory      28959..28987
                     /regulatory_class="terminator"
                     /gene="aphA"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="aphA Terminator"
     regulatory      complement(28959..28987)
                     /regulatory_class="terminator"
                     /operon="traGFEDCBA"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="tra Terminator"
     misc_feature    28994..42276
                     /SECDrawAs="Region"
                     /note="region annotated based on pTA-Mob (Strand et al.,
                     2014)"
                     /label="Tra1"
     CDS             complement(28994..29284)
                     /gene="traA"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraA protein"
                     /protein_id="CAJ85717.1"
                     /db_xref="InterPro:IPR020497; UniProtKB/TrEMBL:A6H969"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="putatively involved in conjugative DNA
                     transfer"
                     /label="traA"
     gene            complement(28994..29284)
                     /gene="traA"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(29292..29732)
                     /gene="traB"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB protein"
                     /protein_id="CAJ85718.1"
                     /db_xref="UniProtKB/TrEMBL:A6H970"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="putatively involved in conjugative DNA
                     transfer"
                     /label="traB"
     gene            complement(29292..29732)
                     /gene="traB"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(29748..32933)
                     /gene="traC1"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraC DNA primase and ssDNA binding protein,
                     longform"
                     /protein_id="CAJ85719.1"
                     /db_xref="GOA:A6H971; InterPro:IPR006171;
                     InterPro:IPR013610; UniProtKB/TrEMBL:A6H971"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="required for conjugative DNA transfer; ssDNA
                     binding protein; antirestriction protein"
                     /label="traC1"
     CDS             complement(29748..31988)
                     /gene="traC2"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraC DNA primase, short form"
                     /protein_id="CAJ85720.1"
                     /db_xref="GOA:A6H972; InterPro:IPR006171;
                     UniProtKB/TrEMBL:A6H972"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traC2"
     gene            complement(29748..32933)
                     /gene="traC1"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     gene            complement(29748..31988)
                     /gene="traC2"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     32783..32802
                     /Sequence="TGTAACGCTTCCCGGTAGTC"
                     /Hairpin_Tm="40.3"
                     /Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="6.1"
                     /Product_Size=6295
                     /label="Fragment_7_F"
     misc_feature    32783..39077
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 7"
     CDS             complement(32940..33203)
                     /gene="traD"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraD protein"
                     /protein_id="CAJ85721.1"
                     /db_xref="InterPro:IPR016703; UniProtKB/TrEMBL:A6H973"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="putatively involved in conjugative DNA
                     transfer"
                     /label="traD"
     gene            complement(32940..33203)
                     /gene="traD"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     complement(33189..33208)
                     /Sequence="AAGCGATGAATGATCCCAAG"
                     /Hairpin_Tm="None"
                     /Tm="55.9"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6204
                     /label="Fragment_6_R"
     CDS             complement(33209..35422)
                     /gene="traE"
                     /operon="traGFEDCBA"
                     /codon_start=1
                     /transl_table=11
                     /product="TraE topoisomerase"
                     /protein_id="CAJ85722.1"
                     /db_xref="GOA:A6H974; InterPro:IPR000380;
                     InterPro:IPR003601; InterPro:IPR003602;
                     InterPro:IPR005738; InterPro:IPR006171;
                     InterPro:IPR013497; InterPro:IPR013498;
                     InterPro:IPR013824; InterPro:IPR013825;
                     UniProtKB/TrEMBL:A6H974"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in conjugative DNA transfer;
                     relaxation ofnegatively supercoiled DNA; Topoisomerase IA"
                     /note="also known as topB"
                     /label="traE"
     gene            complement(33209..35422)
                     /gene="traE"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(35437..35970)
                     /gene="traF"
                     /operon="traGFEDCBA"
                     /function="mating pair formation; conjugative transfer
                     signal peptidase"
                     /codon_start=1
                     /transl_table=11
                     /product="TraF protease"
                     /protein_id="CAJ85723.1"
                     /db_xref="InterPro:IPR011056; InterPro:IPR014139;
                     InterPro:IPR015927; InterPro:IPR019533;
                     UniProtKB/TrEMBL:A6H975"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as pilB"
                     /label="traF"
     gene            complement(35437..35970)
                     /gene="traF"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    35686..35698
                     /note="OB5"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B5"
     CDS             complement(35967..37874)
                     /gene="traG"
                     /operon="traGFEDCBA"
                     /function="conjugal transfer coupling protein; VirD4
                     homologue"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG protein"
                     /protein_id="CAJ85724.1"
                     /db_xref="GOA:A6H976; InterPro:IPR003688;
                     UniProtKB/TrEMBL:A6H976"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as pilA"
                     /label="traG"
     gene            complement(35967..37874)
                     /gene="traG"
                     /operon="traGFEDCBA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     operon          complement(<37871..40574)
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="95.31%"
                     /label="traJXIH operon"
     CDS             complement(37871..40069)
                     /gene="traI"
                     /operon="traJXIH"
                     /codon_start=1
                     /transl_table=11
                     /product="TraI DNA relaxase"
                     /protein_id="CAJ85725.1"
                     /db_xref="InterPro:IPR005094; UniProtKB/TrEMBL:A6H977"
                     /Transferred_Translation="MIAKHVPMRSIKKSDFAELVKYITDEQGKTER
                     LGHVRVTNCEANTLPAVMAEVMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIE
                     DRICAGLGFAEHQRVSAVHHDTDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATL
                     ERDYGLERDNHETRQRVSENRANDMERHAGVESLVGWIKRECLPELQAAQSWEDLHRV
                     LRENGLKLRERGNGFIFEAGDGTTVKASTVSRDLSKPKLEARFGAFTPAEGGEAPRRR
                     EYRAKPLKTRIDTTELYARYQSERQEMGAVRKGELDTLRRRRDRLIEAAMRSNRLRRA
                     AIKLLGEGRIAKRLMYAQAHKALRADLDKINREYRQGRQAVQERTQRRAWADWLKAEA
                     MKGDDKALAALRAREGRSDLKGNTIQGSGEAKPGHAAVTDNITKKGTIIYRVGSSAVR
                     DDGDRLQVSREATTDGLDAALRLAMERFGDRITVNGTAEFKERIAQAAAAGRLAITFD
                     DAALERRRQELLTKEQAHEQPERNDGRRDRGGDGGIRPAAARTTLNATGGDGDRRDAR
                     AVSAGGTVALRKPNVGRIGRKPPPQSQNRLRALSQLGVVRIAGGAEMLLPRDVPGHVE
                     QQGAEPAHALRRGVSGPGRGLKPEQIAAAEKYVAEREQKRLNGFDIPKHARYTDYVGA
                     LSYAGTRNVEDQALALLRKENDEILVLPVDKATVQRMKRLAIGDPVTVTPRGSLKTTR
                     GRSR"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.95%"
                     /note="also known as mobB; TraH, TraI, and TraJ interact
                     at oriT to form the relaxosome nucleoprotein complex and
                     initiate transfer DNA synthesis"
                     /function="conjugal transfer relaxase; endonuclease
                     relaxase; VirD2 homologue mediates cleavage at the nick
                     site of oriT; pfam03432"
                     /label="traI"
     gene            complement(37871..40069)
                     /gene="traI"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.95%"
     protein_bind    38072..38085
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      complement(38075..38080)
                     /regulatory_class="minus_10_signal"
                     /gene="traI"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traI -10"
     protein_bind    38091..38104
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      complement(38098..38103)
                     /regulatory_class="minus_35_signal"
                     /gene="traI"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traI -35"
     CDS             complement(38170..38529)
                     /gene="traH"
                     /operon="traJXIH"
                     /function="relaxosome stabilisation"
                     /codon_start=1
                     /transl_table=11
                     /product="TraH protein"
                     /protein_id="CAJ85726.1"
                     /db_xref="UniProtKB/TrEMBL:A6H978"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="TraH, TraI, and TraJ interact at oriT to form the
                     relaxosome nucleoprotein complex and initiate transfer DNA
                     synthesis"
                     /label="traH"
     gene            complement(38170..38529)
                     /gene="traH"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     38624..38643
                     /Sequence="GATCCGCTCCTTGAACTCTG"
                     /Hairpin_Tm="None"
                     /Tm="58.1"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6256
                     /label="Fragment_8_F"
     misc_feature    38624..44879
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 8"
     primer_bind     complement(39058..39077)
                     /Sequence="CATTGCAAAGCGACTGATGT"
                     /Hairpin_Tm="None"
                     /Tm="57.4"
                     /Self_Dimer_Tm="1.7"
                     /%GC="45.0"
                     /Pair_Dimer_Tm="6.1"
                     /Product_Size=6295
                     /label="Fragment_7_R"
     CDS             complement(40066..40107)
                     /gene="traX"
                     /operon="traJXIH"
                     /function="translational regulation of tra gene
                     expression; translational coupling"
                     /codon_start=1
                     /transl_table=11
                     /product="TraX"
                     /protein_id="CAJ85727.1"
                     /db_xref="UniProtKB/TrEMBL:A6H979"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as orfX"
                     /label="traX"
     gene            complement(40066..40107)
                     /gene="traX"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    40087..40099
                     /note="OB4"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B4"
     CDS             complement(40104..40475)
                     /gene="traJ"
                     /operon="traJXIH"
                     /codon_start=1
                     /transl_table=11
                     /product="TraJ conjugal transfer relaxosome component"
                     /protein_id="CAJ85728.1"
                     /db_xref="UniProtKB/TrEMBL:A6H980"
                     /Transferred_Translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAA
                     AAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARF
                     GDATILALLAKIEEKQDELGKVMMGVVRPRAEP"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.73%"
                     /note="also known as mobA; TraH, TraI, and TraJ interact
                     at oriT to form the relaxosome nucleoprotein complex and
                     initiate transfer DNA synthesis"
                     /function="oriT recognising protein"
                     /label="traJ"
     gene            complement(40104..40475)
                     /gene="traJ"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.73%"
     regulatory      complement(40516..40521)
                     /regulatory_class="minus_10_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traJXIH -10"
     regulatory      complement(40539..40544)
                     /regulatory_class="minus_35_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traJXIH -35"
     protein_bind    40541..40554
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      complement(40546..40551)
                     /regulatory_class="minus_10_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traJXIH -10"
     operon          40563..42325
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.94%"
                     /label="traKLM operon"
     regulatory      40563..40568
                     /regulatory_class="minus_35_signal"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traKLM -35"
     protein_bind    40564..40577
                     /operon="traKLM"
                     /bound_moiety="TrbA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="TrbA binding site"
     regulatory      complement(40569..40574)
                     /regulatory_class="minus_35_signal"
                     /operon="traJXIH"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traJXIH -35"
     regulatory      40586..40591
                     /regulatory_class="minus_10_signal"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traKLM -10"
     CDS             40713..41117
                     /gene="traK"
                     /operon="traKLM"
                     /codon_start=1
                     /transl_table=11
                     /product="TraK oriT-binding protein"
                     /protein_id="CAJ85729.1"
                     /db_xref="UniProtKB/TrEMBL:A6H981"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="DNA processing protein"
                     /label="traK"
     gene            40713..41117
                     /gene="traK"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             41117..41842
                     /gene="traL"
                     /operon="traKLM"
                     /function="DNA processing protein; ATPase involved in
                     chromosome partitioning"
                     /codon_start=1
                     /transl_table=11
                     /product="TraL protein"
                     /protein_id="CAJ85730.1"
                     /db_xref="InterPro:IPR002586; UniProtKB/TrEMBL:A6H982"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traL"
     gene            41117..41842
                     /gene="traL"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             41839..42276
                     /gene="traM"
                     /operon="traKLM"
                     /function="involved in conjugative DNA transfer;
                     increasestransfer frequency"
                     /codon_start=1
                     /transl_table=11
                     /product="TraM protein"
                     /protein_id="CAJ85731.1"
                     /db_xref="UniProtKB/TrEMBL:A6H983"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="traM"
     gene            41839..42276
                     /gene="traM"
                     /operon="traKLM"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     operon          complement(42295..>44051)
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.89%"
                     /label="kfrBC operon"
     regulatory      42295..42325
                     /regulatory_class="terminator"
                     /operon="traKLM"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="96.77%"
                     /label="traKLM Terminator"
     regulatory      complement(42295..42325)
                     /regulatory_class="terminator"
                     /operon="krfBC"
                     /note="bi-directional"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="96.77%"
                     /label="kfrBC Terminator"
     CDS             complement(42329..43675)
                     /gene="kfrC"
                     /operon="krfBC"
                     /function="possible plasmid maintenance; stable
                     inheritance protein; phosphoribosyl transferase (PRT)-type
                     I domain"
                     /note="also known as traN, upf54.4"
                     /codon_start=1
                     /transl_table=11
                     /product="KfrC protein"
                     /protein_id="CAJ85732.1"
                     /db_xref="GOA:A6H984; InterPro:IPR000836;
                     UniProtKB/TrEMBL:A6H984"
                     /Transferred_Translation="MSSYSRAERAPWGDFPKVVRNGDLGSLTNEPE
                     YQAAKQGDAEAALNLVERLISDDTVAQLKTLIGDDKPRIVPVLAVEAAGNNKIPAMMA
                     VVLADRLGLEVETDIVQREKVARTGAGSDHRLAFNPTFEGEVIPGQKYIVVDDTLTMG
                     GTIASLRGYIENNGGKVMAASVMTAHEGALDLAVKPKMLAGINEKHGPAMDAFWKETF
                     GYGIDRLTQGEAGHLRAAPSVDAIRDRIAAARNEAVNRVGASRTATTARAGQQQSPAV
                     KQSSGNSGEDLLQAAQEAETEQQALLESAPIEQTYQQTLALYVQAKHDQVERIEDRLE
                     QLVDRQQARLQQLQSNAPGLLSLPRTKAAWQQQQAQQQARLQTLHTRLDMVREIKEGM
                     GIHAPKIEELATRKMRAEHPELAGDWDSMREAARRHQALMRKQEQEKKQAQERERGRS
                     QSLGLSNKPS"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.93%"
                     /label="kfrC"
     gene            complement(42329..43675)
                     /gene="kfrC"
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.93%"
     protein_bind    43688..43700
                     /note="OB3"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B3"
     CDS             complement(43704..44051)
                     /gene="kfrB"
                     /operon="krfBC"
                     /function="possible plasmid maintenance; stable
                     inheritance protein"
                     /note="also known as traO, upf54.8"
                     /codon_start=1
                     /transl_table=11
                     /product="KfrB protein"
                     /protein_id="CAJ85733.1"
                     /db_xref="UniProtKB/TrEMBL:A6H985"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kfrB"
     gene            complement(43704..44051)
                     /gene="kfrB"
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(43831..43836)
                     /regulatory_class="minus_10_signal"
                     /gene="krfC"
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="krfC -10"
     gene            order(complement(43855..43860),complement(43831..43836))
                     /gene="krfC"
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(43855..43860)
                     /regulatory_class="minus_35_signal"
                     /gene="krfC"
                     /operon="krfBC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="krfC -35"
     regulatory      complement(44109..44145)
                     /regulatory_class="terminator"
                     /gene="krfA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="krfA Terminator"
     gene            complement(44109..44145)
                     /gene="krfA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(44180..44185)
                     /regulatory_class="minus_10_signal"
                     /gene="krfB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="krfB -10"
     gene            order(complement(44203..44208),complement(44180..44185))
                     /gene="krfB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(44203..44208)
                     /regulatory_class="minus_35_signal"
                     /gene="krfB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="krfB -35"
     misc_feature    44209..48599
                     /SECDrawAs="Region"
                     /note="region annotated based on pTA-Mob (Strand et al.,
                     2014)"
                     /label="RK2 central control region"
     CDS             complement(44209..45135)
                     /gene="kfrA"
                     /function="autoregulation; possible plasmid maintainance;
                     putatively involved in stable inheritance"
                     /codon_start=1
                     /transl_table=11
                     /product="KfrA coiled-coil protein"
                     /protein_id="CAJ85734.1"
                     /db_xref="InterPro:IPR021104; UniProtKB/TrEMBL:A6H986"
                     /Transferred_Translation="MELNKETRDRIFAAADELFEQGDRENFPTVDA
                     VRKAARVNMNDASAGMREWRRQRTAQAAPLAVQVPDAVQQAGNQAVAALWQAAQALAN
                     ESLQAAQAGWDRERNELEAVRGELADAFEAQARELEEAQARVTLLEQQAAEAAELAAR
                     QRQALAEARTALAGAEQRAALATQRADEVERRAQELRAELDYAHQDARAFKEESRKTL
                     DAANQEMQALRGERDATRREAESLRTELAAVKAKAQADAEAHQEQRKLAAQEAARQAE
                     RFTTVQAERDEARQEAKAAQEEAAMLRGRLDAETSGRKHKAGGK"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.89%"
                     /label="kfrA"
     gene            order(complement(45186..45191),complement(45162..45167),
                     complement(44209..45135))
                     /gene="kfrA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.89%"
     primer_bind     44536..44575
                     /Sequence="TTCTTTGAATGCGCGGGCGTCCTGGTGAGCGTAGTCCAGC"
                     /Hairpin_Tm="43.1"
                     /Tm="72.5"
                     /Self_Dimer_Tm="21.7"
                     /%GC="60.0"
                     /Pair_Dimer_Tm="21.6"
                     /Product_Size=5999
                     /label="Fragment_9_F"
     misc_feature    44536..50534
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 9"
     primer_bind     complement(44862..44879)
                     /Sequence="AGGCCCTTGCCAATGAAT"
                     /Hairpin_Tm="61.5"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=6256
                     /label="Fragment_8_R"
     protein_bind    complement(45152..45175)
                     /bound_moiety="KfrA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="KfrA binding site"
     regulatory      complement(45162..45167)
                     /regulatory_class="minus_10_signal"
                     /gene="kfrA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="kfrA -10"
     regulatory      complement(45186..45191)
                     /regulatory_class="minus_35_signal"
                     /gene="kfrA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="kfrA -35"
     protein_bind    45195..45204
                     /note="OA2; base change from consensus"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A2"
     protein_bind    45230..45242
                     /note="OB2; base change from consensus"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B2"
     operon          complement(45251..48684)
                     /operon="incC,korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.97%"
                     /label="incC,korABFG operon"
     regulatory      complement(45251..45279)
                     /regulatory_class="terminator"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100%"
                     /label="incC/korABFG Terminator"
     CDS             complement(45321..45848)
                     /gene="korG"
                     /operon="incC, korABFG"
                     /codon_start=1
                     /transl_table=11
                     /product="KorG"
                     /protein_id="CAJ85735.1"
                     /db_xref="UniProtKB/TrEMBL:A6H987"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="host killing override; histone-like protein"
                     /label="korG"
     gene            complement(45321..45848)
                     /gene="korG"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(45858..46361)
                     /gene="korF"
                     /operon="incC, korABFG"
                     /codon_start=1
                     /transl_table=11
                     /product="KorF repressor protein"
                     /protein_id="CAJ85736.1"
                     /db_xref="UniProtKB/TrEMBL:A6H988"
                     /Transferred_Translation="MNPMLFYIAGGVGAALLLVSAIMLFKLREPKK
                     EHRPQRKAAAPTPQPVDNELLRTFRRPSQASQPEPSTTPPARRTAPPSSEQSRGNDGD
                     FVTSALVAGGDQQHHAGLPGRRLADRGHAGRCAAAGYAVCGVRGPLAVLRQQRHGQRL
                     DRYRQLLRFQFVRHRYEQLVRADHD"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.81%"
                     /function="transcriptional repression; histone-like
                     protein"
                     /label="korF"
     gene            complement(45858..46361)
                     /gene="korF"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="99.81%"
     CDS             complement(46432..47508)
                     /gene="korB"
                     /operon="incC, korABFG"
                     /function="global transcriptional repressor; parB-like
                     plasmid partitioning protein; repressor of
                     oriV-replication-initiator trfA"
                     /codon_start=1
                     /transl_table=11
                     /product="KorB partitioning and repressor protein"
                     /protein_id="CAJ85737.1"
                     /db_xref="GOA:A6H989; InterPro:IPR003115;
                     InterPro:IPR004437; InterPro:IPR008988;
                     InterPro:IPR010575; InterPro:IPR013741;
                     UniProtKB/TrEMBL:A6H989"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="korB"
     gene            complement(46432..47508)
                     /gene="korB"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(47505..48599)
                     /gene="incC"
                     /operon="incC, korABFG"
                     /note="long form IncC1, alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="IncC partitioning protein"
                     /protein_id="CAJ85738.1"
                     /db_xref="InterPro:IPR002586; UniProtKB/TrEMBL:A6H990"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="parA family ATPase involved in chromosome
                     partitioning"
                     /label="incC1"
     CDS             complement(47505..48284)
                     /gene="incC"
                     /operon="incC, korABFG"
                     /note="short form IncC2, alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="IncC partitioning protein"
                     /protein_id="CAJ85739.1"
                     /db_xref="InterPro:IPR002586; UniProtKB/TrEMBL:A6H991"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="parA family ATPase involved in chromosome
                     partitioning"
                     /label="incC2"
     gene            complement(47505..48599)
                     /gene="incC"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(48281..48586)
                     /gene="korA"
                     /operon="incC, korABFG"
                     /function="global transcriptional repressor; host killing
                     override"
                     /codon_start=1
                     /transl_table=11
                     /product="KorA repressor protein"
                     /protein_id="CAJ85740.1"
                     /db_xref="UniProtKB/TrEMBL:A6H992"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="korA"
     gene            complement(48281..48586)
                     /gene="korA"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(48656..48661)
                     /regulatory_class="minus_10_signal"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="incC/korABFG -10"
     protein_bind    48658..48667
                     /note="OA1"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A1"
     regulatory      complement(48679..48684)
                     /regulatory_class="minus_35_signal"
                     /operon="incC, korABFG"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="incC/korABFG -35"
     protein_bind    48689..48701
                     /note="OB1"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B1"
     operon          complement(<48760..51710)
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="klaABC operon"
     CDS             complement(48760..49713)
                     /gene="klaC"
                     /function="involved in plasmid maintenance; host-lethal
                     (Kil+) phenotype in the absence of kor functions;
                     tellurite resistance and inhibition of IncW plasmid
                     fertility"
                     /operon="klaABC"
                     /transl_table=11
                     /product="KlaC protein"
                     /protein_id="CAJ85665.1"
                     /db_xref="InterPro:IPR007039; UniProtKB/TrEMBL:A6H917"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="klaC"
     gene            complement(48760..49713)
                     /gene="klaC"
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(49710..50846)
                     /gene="klaB"
                     /function="putative tellurique resistant protein
                     (KlaB/TelA); involved in plasmid maintenance; tellurite
                     resistance and inhibition of IncW plasmid fertility;
                     Uncharacterized protein involved in tellurite resistance;
                     host-lethal (Kil+) phenotype in the absence of kor
                     functions"
                     /operon="klaABC"
                     /codon_start=1
                     /transl_table=11
                     /product="KlaB protein"
                     /protein_id="CAJ85666.1"
                     /db_xref="InterPro:IPR008863; UniProtKB/TrEMBL:A6H918"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /note="also known as telA"
                     /label="klaB"
     gene            complement(49710..50846)
                     /gene="klaB"
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     primer_bind     50162..50180
                     /Sequence="AATGTTGCAAGGCGATCAG"
                     /Hairpin_Tm="None"
                     /Tm="56.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=4928
                     /label="Fragment_10_F"
     misc_feature    50162..55089
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 10"
     primer_bind     complement(50495..50534)
                     /Sequence="CGTTCCCGCCTGCCCCTGATTGGCCCGCTGATCGACCGCT"
                     /Hairpin_Tm="45.1"
                     /Tm="76.9"
                     /Self_Dimer_Tm="None"
                     /%GC="70.0"
                     /Pair_Dimer_Tm="21.6"
                     /Product_Size=5999
                     /label="Fragment_9_R"
     CDS             complement(50864..51637)
                     /gene="klaA"
                     /operon="klaABC"
                     /codon_start=1
                     /transl_table=11
                     /product="KlaA protein"
                     /protein_id="CAJ85667.1"
                     /db_xref="InterPro:IPR008863; UniProtKB/TrEMBL:A6H919"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="putative tellurium resistant protein
                     (KlaA/kilA); involved in plasmid maintenance; tellurite
                     resistance and inhibition of IncW plasmid fertility;
                     host-lethal (Kil+) phenotype in the absence of kor
                     functions"
                     /note="also known as fiwB"
                     /label="klaA"
     gene            complement(50864..51637)
                     /gene="klaA"
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(51681..51686)
                     /regulatory_class="minus_10_signal"
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="klaABC -10"
     protein_bind    51683..51692
                     /note="OA7"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A7"
     regulatory      complement(51705..51710)
                     /regulatory_class="minus_35_signal"
                     /operon="klaABC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="klaABC -35"
     protein_bind    51715..51727
                     /note="OB13"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B13"
     operon          complement(<51760..53080)
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleCDEF operon"
     CDS             complement(51760..52074)
                     /gene="kleF"
                     /operon="kleCDEF"
                     /note="long form KleF1; alternative start codon; involved
                     in plasmid maintenance"
                     /codon_start=1
                     /transl_table=11
                     /product="KleF protein"
                     /protein_id="CAJ85668.1"
                     /db_xref="UniProtKB/TrEMBL:A6H920"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="stable inheritance protein; cannot be
                     maintained in Escherichia coli host cells, unless korA or
                     korC is also present in to control its expression"
                     /note="also known as kcrB4"
                     /label="kleF1"
     CDS             complement(51760..51930)
                     /gene="kleF"
                     /operon="kleCDEF"
                     /note="short form KleF2, alternative start codon"
                     /codon_start=1
                     /transl_table=11
                     /product="KleF protein"
                     /protein_id="CAJ85669.1"
                     /db_xref="UniProtKB/TrEMBL:A6H921"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="plasmid maintenance; stable inheritance
                     protein; cannot be maintained in Escherichia coli host
                     cells, unless korA or korC is also present to control its
                     expression"
                     /note="also known as kcrB4"
                     /label="kleF2"
     gene            complement(51760..52074)
                     /gene="kleF"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(52102..52425)
                     /gene="kleE"
                     /operon="kleCDEF"
                     /codon_start=1
                     /transl_table=11
                     /product="KleE protein"
                     /protein_id="CAJ85670.1"
                     /db_xref="UniProtKB/TrEMBL:A6H922"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in plasmid maintenance; stable
                     inheritance protein; cannot be maintained in Escherichia
                     coli host cells, unless korA or korC is also present to
                     control its expression"
                     /label="kleE"
     gene            complement(52102..52425)
                     /gene="kleE"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(52537..52755)
                     /gene="kleD"
                     /operon="kleCDEF"
                     /codon_start=1
                     /transl_table=11
                     /product="KleD protein"
                     /protein_id="CAJ85671.1"
                     /db_xref="UniProtKB/TrEMBL:A6H923"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in plasmid maintenance; cannot be
                     maintained in Escherichia coli host cells, unless korA or
                     korC is also present to control its expression"
                     /note="also known as kcrB2"
                     /label="kleD"
     gene            complement(52537..52755)
                     /gene="kleD"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(52771..53001)
                     /gene="kleC"
                     /operon="kleCDEF"
                     /codon_start=1
                     /transl_table=11
                     /product="KleC protein"
                     /protein_id="CAJ85672.1"
                     /db_xref="UniProtKB/TrEMBL:A6H924"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in plasmid maintenance; cannot be
                     maintained in Escherichia coli host cells, unless korA or
                     korC is also present to control its expression"
                     /note="also known as kcrB1"
                     /label="kleC"
     gene            complement(52771..53001)
                     /gene="kleC"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(53051..53056)
                     /regulatory_class="minus_10_signal"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleCDEF -10"
     protein_bind    53055..53071
                     /note="OC3"
                     /bound_moiety="KorC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator C3"
     regulatory      complement(53075..53080)
                     /regulatory_class="minus_35_signal"
                     /operon="kleCDEF"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleCDEF -35"
     protein_bind    53086..53095
                     /note="OA6"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A6"
     operon          complement(<53154..53735)
                     /operon="kleAB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleAB operon"
     CDS             complement(53154..53369)
                     /gene="kleB"
                     /operon="kleAB"
                     /codon_start=1
                     /transl_table=11
                     /product="KleB protein"
                     /protein_id="CAJ85673.1"
                     /db_xref="UniProtKB/TrEMBL:A6H925"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in plasmid maintenance; Cannot be
                     maintained in Escherichia coli host cells, unless korA or
                     korC is also present to control its expression"
                     /note="also known as kcrA2"
                     /label="kleB"
     gene            complement(53154..53369)
                     /gene="kleB"
                     /operon="kleAB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     CDS             complement(53418..53651)
                     /gene="kleA"
                     /operon="kleAB"
                     /codon_start=1
                     /transl_table=11
                     /product="KleA protein"
                     /protein_id="CAJ85674.1"
                     /db_xref="UniProtKB/TrEMBL:A6H926"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="involved in plasmid maintenance; porin
                     superfamily"
                     /note="also known as kcrA1"
                     /label="kleA"
     gene            complement(53418..53651)
                     /gene="kleA"
                     /operon="kleAB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     regulatory      complement(53706..53711)
                     /regulatory_class="minus_10_signal"
                     /operon="kleAB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleAB -10"
     protein_bind    53710..53726
                     /note="OC2"
                     /bound_moiety="KorC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator C2"
     regulatory      complement(53730..53735)
                     /regulatory_class="minus_35_signal"
                     /operon="kleAB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="kleAB -35"
     protein_bind    53741..53750
                     /note="OA5"
                     /bound_moiety="KorA"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator A5"
     regulatory      complement(53771..53796)
                     /regulatory_class="terminator"
                     /gene="korC"
                     /operon="possible operon for bla and korC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="korC Terminator"
     gene            order(complement(53877..54134),complement(53771..53796))
                     /gene="korC"
                     /operon="possible operon for bla and korC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     protein_bind    53832..53844
                     /note="OB12"
                     /bound_moiety="KorB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /label="Operator B12"
     CDS             complement(53877..54134)
                     /gene="korC"
                     /operon="possible operon for bla and korC"
                     /codon_start=1
                     /transl_table=11
                     /product="KorC repressor protein"
                     /protein_id="CAJ85675.1"
                     /db_xref="InterPro:IPR020136; UniProtKB/TrEMBL:A6H927"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /function="transcriptional regulator; acts with KorA as
                     corepressor in the control of the kilC and kilE operons"
                     /note="also known as klcC"
                     /label="korC"
     misc_feature    complement(54157..>54831)
                     /gene="klcB"
                     /operon="putative klcAB operon"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="PSEUDO:CAJ85676.1"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="67.87%"
                     /function="involved in stable inheritance; expression is
                     decreased in the presence of KorA and KorC"
                     /note="disrupted by insertion of Tn1 transposon"
                     /label="klcB"
                     /note="Geneious type: truncated protein"
     gene            complement(54157..>54831)
                     /gene="klcB"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="67.87%"
     primer_bind     54783..54822
                     /Sequence="TGCCGCCGCGCGCATGGTCGTAATGGGACCGATAGCCCGT"
                     /Hairpin_Tm="46.7"
                     /Tm="76.8"
                     /Self_Dimer_Tm="29.7"
                     /%GC="67.5"
                     /Pair_Dimer_Tm="7.5"
                     /Product_Size=5791
                     /label="Fragment_1_F"
     misc_feature    54783..4123
                     /note="PCR amplicon for rapid plasmid synthesis"
                     /SECDrawAs="Region"
                     /label="Fragment 1"
     repeat_region   54831..54835
                     /rpt_type="DIRECT"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
     mobile_element  54836..>55232
                     /mobile_element_type="transposon:Tn1"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="44.33%"
                     /note="Tn1 transposon present in ancestral RK2/RP4
                     plasmid; disrupted by instertion of GMr and pBBR1 replicon
                     regions to make pTA-Mob"
                     /label="Tn1"
     repeat_region   54836..54873
                     /note="left inverted repeat"
                     /rpt_type="INVERTED; TERMINAL"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="100.00%"
                     /mobile_element_type="transposon:Tn1"
     misc_feature    complement(54983..>55232)
                     /gene="bla"
                     /operon="possible operon for bla and korC"
                     /function="penicillin resistance"
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="PSEUDO:CAJ85677.1"
                     /db_xref="GOA:A6H929; InterPro:IPR000871;
                     InterPro:IPR001466; InterPro:IPR012338;
                     UniProtKB/TrEMBL:A6H929"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="57.84%"
                     /note="Tn1 bla disrupted by addition of gentamicin
                     resistance marker"
                     /label="bla"
                     /note="Geneious type: truncated protein"
     gene            complement(54983..>55232)
                     /gene="bla"
                     /operon="possible operon for bla and korC"
                     /Transferred_From="BN000925"
                     /Transferred_Similarity="57.84%"
     primer_bind     complement(55070..55089)
                     /Sequence="AGCCCTCCCGTATCGTAGTT"
                     /Hairpin_Tm="None"
                     /Tm="60.1"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=4928
                     /label="Fragment_10_R"
     misc_feature    55233..56187
                     /note="region cloned from pBBR1MCS-5 to make pTA-Mob"
                     /SECDrawAs="Region"
                     /label="GMr region"
     CDS             complement(55306..55839)
                     /function="gentamicin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="gentamicin-3-acetyltransferase"
                     /protein_id="AAB06696.1"
                     /Transferred_Translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRL
                     GPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGA
                     LAAYVLPRFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADY
                     GDDPAVALYTKLGIREEVMHFDIDPSTAT"
                     /Transferred_From="XXU25061"
                     /Transferred_Similarity="99.63%"
                     /label="aacC1"
     gene            complement(55306..55839)
                     /gene="aacC1"
     misc_feature    56199..1490
                     /SECDrawAs="Region"
                     /label="pBBR1 replicon"
ORIGIN      
        1 cggcgagtgc gggggttcca agggggcagc gccaccttgg gcaaggccga aggccgcgca
       61 gtcgatcaac aagccccgga ggggccactt tttgccggag ggggagccgc gccgaaggcg
      121 tgggggaacc ccgcaggggt gcccttcttt gggcaccaaa gaactagata tagggcgaaa
      181 tgcgaaagac ttaaaaatca acaacttaaa aaaggggggt acgcaacagc tcattgcggc
      241 accccccgca atagctcatt gcgtaggtta aagaaaatct gtaattgact gccactttta
      301 cgcaacgcat aattgttgtc gcgctgccga aaagttgcag ctgattgcgc atggtgccgc
      361 aaccgtgcgg cacccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg
      421 cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc
      481 gtgggccggg cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc
      541 gcagatgggc caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg
      601 acgttctttg cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc
      661 cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc
      721 gtggggccag ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga
      781 tcacgacgac caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct
      841 gtatccgggc gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg
      901 cattccgggc atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg
      961 cgggcagcag cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt
     1021 ggagccgccg acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg
     1081 taagtgcgct gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc
     1141 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     1201 gcacctcgct aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat
     1261 atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa
     1321 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     1381 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     1441 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa cttaattaat
     1501 aagagcttgg tgagcgctag gagtcactgc caggtatcgt ttgaacacgg cattagtcag
     1561 ggaagtcata acacagtcct ttcccgcaat tttctttttc tattactctt ggcctcctct
     1621 agtacactct atattttttt atgcctcggt aatgattttc attttttttt tttccaccta
     1681 gcggatgact cttttttttt cttagcgatt ggcattatca cataatgaat tatacattat
     1741 ataaagtaat gtgatttctt cgaagaatat actaaaaaat gagcaggcaa gataaacgaa
     1801 ggcaaagatg acagagcaga aagccctagt aaagcgtatt acaaatgaaa ccaagattca
     1861 gattgcgatc tctttaaagg gtggtcccct agcgatagag cactcgatct tcccagaaaa
     1921 agaggcagaa gcagtagcag aacaggccac acaatcgcaa gtgattaacg tccacacagg
     1981 tatagggttt ctggaccata tgatacatgc tctggccaag cattccggct ggtcgctaat
     2041 cgttgagtgc attggtgact tacacataga cgaccatcac accactgaag actgcgggat
     2101 tgctctcggt caagctttta aagaggccct aggggccgtg cgtggagtaa aaaggtttgg
     2161 atcaggattt gcgcctttgg atgaggcact ttccagagcg gtggtagatc tttcgaacag
     2221 gccgtacgca gttgtcgaac ttggtttgca aagggagaaa gtaggagatc tctcttgcga
     2281 gatgatcccg cattttcttg aaagctttgc agaggctagc agaattaccc tccacgttga
     2341 ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg ctcttgcggt
     2401 tgccataaga gaagccacct cgcccaatgg taccaacgat gttccctcca ccaaaggtgt
     2461 tcttatgtag tgacaccgat tatttaaagc tgcagcatac gatatatata catgtgtata
     2521 tatgtatacc tatgaatgtc agtaagtatg tatacgaaca gtatgatact gaagatgaca
     2581 aggtaatgca tcattctata cgtgtcattc tgaacgaggc gcgctttcct atcacgtgct
     2641 ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa atagaaagta
     2701 aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaatc tagagactct
     2761 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     2821 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     2881 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     2941 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     3001 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     3061 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     3121 aagttacagg caagcgatcc actagtatct tccgctgcat aaccctgctt cggggtcatt
     3181 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     3241 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     3301 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     3361 aacgggaatc ctgctctgcg aggctggccg gctaccgccg gcgtaacaga tgagggcaag
     3421 cggatggctg atgaaaccaa gccaaccagg aagggcagcc cacctatcaa ggtgtactgc
     3481 cttccagacg aacgaagagc gattgaggaa aaggcggcgg cggccggcat gagcctgtcg
     3541 gcctacctgc tggccgtcgg ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac
     3601 gtccgcgagc tggcccgcat caatggcgac ctgggccgcc tgggcggcct gctgaaactc
     3661 tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg
     3721 gcgaagatcg aagagaagca ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg
     3781 agggcagagc catgactttt ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa
     3841 gcacgtcccc atgcgctcca tcaagaagag gcacttcgag ctgtaagtac atcaccgacg
     3901 agcaaggcaa gacgatcgag ctctaggagt gcggttggaa cgttggccca gccagatact
     3961 cccgatcacg agcaggacgc cgatgatttg aagcgcactc agcgtctgat ccaagaacaa
     4021 ccatcctagc aacacggcgg tccccgggct gagaaagccc agtaaggaaa caactgtagg
     4081 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
     4141 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
     4201 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
     4261 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
     4321 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
     4381 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
     4441 acctagcaga gcggcagaga taaacacgac catcagcggc tgcacagcgc ctaccgtcgc
     4501 cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat
     4561 attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac
     4621 gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg
     4681 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
     4741 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
     4801 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
     4861 gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa
     4921 atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt
     4981 taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg
     5041 aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct
     5101 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
     5161 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
     5221 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
     5281 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
     5341 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
     5401 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
     5461 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
     5521 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
     5581 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
     5641 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
     5701 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
     5761 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
     5821 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
     5881 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
     5941 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
     6001 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc
     6061 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
     6121 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
     6181 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
     6241 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
     6301 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
     6361 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
     6421 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
     6481 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
     6541 tcctgcttgt cgccggcatc gttgcgccac tcttcattaa ccgctatatc gaaaattgct
     6601 tgcggcttgt tagaattgcc atgacgtacc tcggtgtcac gggtaagatt accgataaac
     6661 tggaactgat tatggctcat atcgaaagtc tccttgagaa aggagactct agtttagcta
     6721 aacattggtt ccgctgtcaa gaactttagc ggctaaaatt ttgcgggccg cgaccaaagg
     6781 tgcgaggggc ggcttccgct gtgtacaacc agatattttt caccaacatc cttcgtctgc
     6841 tcgatgagcg gggcatgacg aaacatgagc tgtcggagag ggcaggggtt tcaatttcgt
     6901 ttttatcaga cttaaccaac ggtaaggcca acccctcgtt gaaggtgatg gaggccattg
     6961 ccgacgccct ggaaactccc ctacctcttc tcctggagtc caccgacctt gaccgcgagg
     7021 cactcgcgga gattgcgggt catcctttca agagcagcgt gccgcccgga tacgaacgca
     7081 tcagtgtggt tttgccgtca cataaggcgt ttatcgtaaa gaaatggggc gacgacaccc
     7141 gaaaaaagct gcgtggaagg ctctgacgcc aagggttagg gcttgcactt ccttctttag
     7201 ccgctaaaac ggccccttct ctgcgggccg tcggctcgcg catcatatcg acatcctcaa
     7261 cggaagccgt gccgcgaatg gcatcgggcg ggtgcgcttt gacagttgtt ttctatcaga
     7321 acccctacgt cgtgcggttc gattagctgt ttgtcttgca ggctaaacac tttcggtata
     7381 tcgtttgcct gtgcgataat gttgctaatg atttgttgcg taggggttac tgaaaagtga
     7441 gcgggaaaga agagtttcag accatcaagg agcgggccaa gcgcaagctg gaacgcgaca
     7501 tgggtgcgga cctgttggcc gcgctcaacg acccgaaaac cgttgaagtc atgctcaacg
     7561 cggacggcaa ggtgtggcac gaacgccttg gcgagccgat gcggtacatc tgcgacatgc
     7621 ggcccagcca gtcgcaggcg attatagaaa cggtggccgg attccacggc aaagaggtca
     7681 cgcggcattc gcccatcctg gaaggcgagt tccccttgga tggcagccgc tttgccggcc
     7741 aattgccgcc ggtcgtggcc gcgccaacct ttgcgatccg caagcgcgcg gtcgccatct
     7801 tcacgctgga acagtacgtc gaggcgggca tcatgacccg cgagcaatac gaggtcatta
     7861 aaagcgccgt cgcggcgcat cgaaacatcc tcgtcattgg cggtactggc tcgggcaaga
     7921 ccacgctcgt caacgcgatc atcaatgaaa tggtcgcctt caacccgtct gagcgcgtcg
     7981 tcatcatcga ggacaccggc gaaatccagt gcgccgcaga gaacgccgtc caataccaca
     8041 ccagcatcga cgtctcgatg acgctgctgc tcaagacaac gctgcgtatg cgccccgacc
     8101 gcatcctggt cggtgaggta cgtggccccg aagcccttga tctgttgatg gcctggaaca
     8161 ccgggcatga aggaggtgcc gccaccctgc acgcaaacaa ccccaaagcg ggcctgagcc
     8221 ggctcgccat gcttatcagc atgcacccgg attcaccgaa acccattgag ccgctgattg
     8281 gcgaggcggt tcatgtggtc gtccatatcg ccaggacccc tagcggccgt cgagtgcaag
     8341 aaattctcga agttcttggt tacgagaacg gccagtacat caccaaaacc ctgtaaggag
     8401 tatttccaat gacaacggct gttccgttcc gtctgaccat gaatcgcggc attttgttct
     8461 accttgccgt gttcttcgtt ctcgctctcg cgttatccgc gcatccggcg atggcctcgg
     8521 aaggcaccgg cggcagcttg ccatatgaga gctggctgac gaacctgcgc aactccgtaa
     8581 ccggcccggt ggccttcgcg ctgtccatca tcggcatcgt cgtcgccggc ggcgtgctga
     8641 tcttcggcgg cgaactcaac gccttcttcc gaaccctgat cttcctggtt ctggtgatgg
     8701 cgctgctggt cggcgcgcag aacgtgatga gcaccttctt cggtcgtggt gccgaaatcg
     8761 cggccctcgg caacggggcg ctgcaccagg tgcaagtcgc ggcggcggat gccgtgcgtg
     8821 cggtagcggc tggacggctc gcctaatcat ggctctgcgc acgatcccca tccgtcgcgc
     8881 aggcaaccga gaaaacctgt tcatgggtgg tgatcgtgaa ctggtgatgt tctcgggcct
     8941 gatggcgttt gcgctgattt tcagcgccca agagctgcgg gccaccgtgg tcggtctgat
     9001 cctgtggttc ggggcgctct atgcgttccg aatcatggcg aaggccgatc cgaagatgcg
     9061 gttcgtgtac ctgcgtcacc gccggtacaa gccgtattac ccggcccgct cgaccccgtt
     9121 ccgcgagaac accaatagcc aagggaagca ataccgatga tccaagcaat tgcgattgca
     9181 atcgcgggcc tcggcgcgct tctgttgttc atcctctttg cccgcatccg cgcggtcgat
     9241 gccgaactga aactgaaaaa gcatcgttcc aaggacgccg gcctggccga tctgctcaac
     9301 tacgccgctg tcgtcgatga cggcgtaatc gtgggcaaga acggcagctt tatggctgcc
     9361 tggctgtacg agggcgatga caacgcaagc agcaccgacc agcagcgcga agtagtgtcc
     9421 gcccgcatca accaggccct cgcgggcctg ggaagtgggt ggatgatcca tgtggacgcc
     9481 gtgcggcgtc ctgctccgaa ctacgcggag cggggcctgt cggcgttccc tgaccgtctg
     9541 acggcagcga ttgaagaaga gcgccggcgg catttcgaga gcctgggaac gatgtacgag
     9601 ggctatttcg tcctcacctt gacctggttc ccgccgctgc tcgcccagcg caagttcgtc
     9661 gagctgatgt ttgacgacga cgcgaccgca ccggatcgca aggcgcgcac gcggggcctc
     9721 atcgaccaat tcaagcgtga cgtgcgcagc atcgagtcgc gcctgtcgtc ggccgtgtcg
     9781 ctcactcgct tgaaggggca caagatcgtc aacgaggacg gcacgaccgt cacgcatgac
     9841 gacttcctgc gctggctgca attctgcgtg acgggcctgc accatccggt gcagctcccc
     9901 agcaacccga tgtacctgga cgccctggtc ggcggacagg aaatgtgggg cggggtagtg
     9961 cccaaggtcg gccgcaagtt cgtccaggtg gtcgctctcg aaggcttccc cttggagtcc
    10021 tatcccggca tcctgacggc gctcggcgag ctgccctgcg agtatcggtg gtcgagccgg
    10081 ttcatcttca tggaccagca cgaagccgtg aagcacctcg acaagttccg caagaagtgg
    10141 cggcagaaga ttcgcggctt cttcgaccag gtgttcaaca cgaacaccgg cccggtcgat
    10201 caggacgcgc tttcgatggt ggccgatgct gaggcggcca ttgccgaagt caacagcggc
    10261 atcgtggccg tgggctacta caccagcgtc gtcgtgctga tggatgagga ccgcacgcgc
    10321 ctggaagctg cggcccgcga tgttgaaaag gccgtcaacc ggttgggctt tgccgcgcgc
    10381 atcgagtcca tcaacaccct ggacgccttc cttggtagtt tgccgggcca cggcgtggaa
    10441 aacgtccgcc ggccgctcat caacacgatg aacctggccg acctgctgcc gaccagcacc
    10501 atctggaccg gcaacgcgaa cgcgccatgc ccgatgtacc cgccgctgtc gccggcgctc
    10561 atgcactgcg tcacgcaagg atcaacgccg ttccggctga acctgcacgt gcgcgacctc
    10621 ggccacacct ttatgttcgg gccgaccggc gcaggtaaat cgacgcacct ggcgatcctc
    10681 gccgcgcagc tccgtcgcta tgccggcatg tcgatcttcg cctttgacaa gggcatgtcg
    10741 atgtacccgc tggccgccgg catccgtgcg gccacgaagg gcaccagcgg cctgcacttc
    10801 accgtggcgg ccgacgacga acgcctggcg ttctgcccgt tgcagttcct gagcaccaag
    10861 ggcgaccgtg cttgggcgat ggagtggatc gacaccatcc tggcgttgaa cggcgtcgaa
    10921 acgaccccgg cccagcgcaa cgaaatcggc aacgcgatca tgagcatgca cgccagcggc
    10981 gcgcgcacgc tctccgagtt cagcgtgacg attcaggatg aggcgatccg cgaggcgatc
    11041 cgccagtaca ccgtcgatgg cgcaatgggc catctgctcg acgccgaaga ggacggcttg
    11101 gcgctgtccg actttacagt gttcgagatc gaagagctga tgaacctcgg cgagaaattc
    11161 gccctgcctg tgttgctcta cctgttccgc cgtatcgagc gcgccctgac gggccagccg
    11221 gccgtcatca tcctggacga agcctggttg atgctcggcc acccggcatt ccgcgcgaag
    11281 atcagggaat ggctcaaggt gctgcgtaag gccaactgcc ttgtgctgat ggcaacgcag
    11341 agcctgtccg acgccgccaa cagcggcatc ctggacgtga tcgtggaatc gaccgcgacc
    11401 aagattttcc tgccgaatat ttacgccagg gatgaggaca cggcggccct gtaccgccgc
    11461 atgggcctga acgctcgcca gatcgagatt ctggcccagg ccgttcccaa gcgtcagtac
    11521 tactacgtgt cggaaaacgg ccgccgtctc tacgacctgg cacttggccc gctcgcgctc
    11581 gcgttcgtcg gcgcatccga caaggaatcc gtcgccatca tcaagaacct ggaagccaag
    11641 ttcggcgacc agtgggtgga tgaatggctg cgtggccggg gcctcgccct tgatgaatac
    11701 ctggaggcag catgagtttt gcagacacga tcaagggctt gatcttcaag aagaagcccg
    11761 caacggccgc agcagcggcg acgccggccg cgaccggccc gcaaaccgac aacccgtacc
    11821 tgacggcgcg gcgcacctgg aacgaccacg ttggttccgt tgtgtcgcaa aagcagacct
    11881 ggcaggttgt cggcatcctt tcgctgatga tcgtcctcgc ggcggtcggc ggcatcatcc
    11941 acatcggcag ccagtcgaag ttcgtgccct atgtctacga ggtagacaag ctcgggcaga
    12001 cggccgccgt ggggccgatg accagggcgt cgaaagccga tccgcgtgtc attcacgcct
    12061 cggtggctga gttcgtcggc gatgctcgcc tggtgacgcc ggacgtagct ttgcagcgca
    12121 aggccgtcta ccgcctctat gccaagctcg ggccgaatga cccggccacc gccaagatga
    12181 acgaatggct caacggcacc gccgacgcca gcccgttcgc tcgcgcggcc gtcgaaacgg
    12241 tcagcaccga aatcacttcc gtaatcccgc agacgcccga cacctggcag gtcgattggg
    12301 tcgagacgac gcgcgacagg caaggcgtgg tgaaaggcca gcccgtgcgc atgcgggcct
    12361 tggtgacggt ctacgtcgtc gagccgacgg cggacaccaa ggaagaacaa ctgcgaaaca
    12421 acccggccgg gatctacgtc cgggacttct cctggtcgag acttctgtga ggcactgaat
    12481 tatgaaaaag gaactgtttg ctttggtcct ggccgcgtcc gttagcgtgc ctgcatttgc
    12541 cgccgatccc ggcgcggacc tgactgacct ctatttttcc ggcaagaacc cggagctgac
    12601 cgcgcaagag cgggcggcca tcgccatcgc caagaagtgg gaggcgggta ccgccggcat
    12661 gcggccggtg gccggccccg gtggttcggt gcgcttcctg ttcggcgcgc agcagccgag
    12721 catcgtatgc gccgtgctgc aagtgtgcga cgtggccctg caacccggcg agcaagtcaa
    12781 ctcgatcaac ctgggcgaca ccgcccgttg gacggtcgag ccggccatta ccggcagcgg
    12841 cgcgaacgaa acccagcacc tcatcatcaa gccgatggat gtgggcctgg aaaccagcct
    12901 ggtcgtgacc acggaccgcc gcagctacca catgcgcctg cgctcgcatc gcacgcagta
    12961 catgccgcag gtgtcgttca cctacccgga agatgccctt gcgaagtggg acgccatcaa
    13021 gaaccgcgaa cagcgggatc gcgtcgagaa aaccattccg cagaccggcg agtacctggg
    13081 caacctgagc ttcaactact ccgtcagcgg gtccacgtcg tggaagccgg tgcgcgtcta
    13141 caacgacggc aagaaaacca tcatccagat gccgcactcg atggaacaga ccgaagcgcc
    13201 gacgctcctg gtcgttcgca gggagggcgg cctgttctcc gacgatgaaa cggtgatggt
    13261 caactaccgg gtccagggcg accgctacat cgtcgatacg attttcgaca aggccatcct
    13321 catcgcgggc gtgggcagca gccaggaccg cgtgaccatt tcaaggggga actaaaccat
    13381 gcgtaagatt ctgaccgtca tcgcactcgc ggccacgttg gccggctgcg cgacctccaa
    13441 gtacggcagc ttcgtccagg acgcgccggc cgcctacaac cagaccattg cgaccgacgc
    13501 ggtgaagcag ctcgtcaagc tctacccgcc ggcgcaaacc aagctggaat tgcagcaggc
    13561 tacgcccgat ccgttcggca ttgccctggt cactgacctt cgcgcccagg gctatgctgt
    13621 catggagtac aagcccgacg gcaacgcggc cgcagctccg gctgctgcgt cctcggccgc
    13681 tgcgaagccg gcaacgccgc aagcccaggg cggctatccg ctgcgctacg tgctggacca
    13741 attcagcgac agcaacctgt atcgcctgac cgtcatggtc ggctctcaat cgctcacgcg
    13801 cgcctacctc gcccaaaaca acacgatggt cccggccggc gcatgggttc ggaaggagta
    13861 agccaatgag cgaagatcaa atggcaccgg acgcatcgcc agatgcggtc aagccgaaaa
    13921 gcggggttcg ccgcgtcaac aacatgccga tgtacctcat cggcggtgtg ctcggcatct
    13981 tcctgctggt gatggccctg gttgctgcgg atcgcgctgc gcagcagaac cagccgggag
    14041 ctgcgaaggc tgagaaggcc ggcagcacca gcatgtttgc cgacgaaatt gccggcaaac
    14101 agcaggacgg catcatcaag gccaagccgc tggagattcc gccggaacaa accgcccagc
    14161 aaccgacgac ggagctgacg ccagccccgg cgcagggaac gactatcacg gtcgcacggc
    14221 ccgagaacct ggaccagccc ccgacgccgc cgcagggtgc gcgcaacgag gacctggacc
    14281 gcatccgcat ggcgaagttg cagatgctgg aagaggcgat caaggccaag acgacggtgc
    14341 gcatcgacgc gccgcgcagc cagggcagcg ccggcggcgg tgctccgcag ggccgcgagg
    14401 aaacccttgc gcgcatccag gagctgcgtc ggcaggctga gaacgcccgc gccaccgatc
    14461 cgaccgccgc ctatcaggcc gcgcttgcgc aggctcgcac gatgggcggc gcggcagggg
    14521 gtggcggtat gggcggctcg ggtgcgccga ccctcgtgca gacctcgaac cgcagtggtg
    14581 gcggcgctgg ctatgggtcg ttcgacaacc gcagcgaggg cgaccgttgg cggctcgact
    14641 cccagccgga agcacctgca acgccctatg tgctgcgcgc tggcttcgtc gttccggcta
    14701 cgcttatctc gggcatcaac tccgatctgc caggccaaat catggcccag gtatcgcagt
    14761 cggtgtacga cacggcgacc ggcaagcaca tgctcatccc ccaaggctcg cgcctggtgg
    14821 gcagctactc gaacgatgtg gcctacgggc agaagcgcgt tctggtggca tggcagcgca
    14881 tcatcttccc cgacggcaag gcaatggaca ttggggccat gccgggcggc gatagcgctg
    14941 ggtatgcagg cttcaacgac aaggtcaaca actactactt ccgcaccttc gcatcggcat
    15001 tcctcatgtc gggcgtcgtt gcgggcatca gcttgagtca ggaccgtggc aacagcaaca
    15061 gcggttacgg acgacaagac gcgggttccg cgatgagtga agcgttgggt caacagctcg
    15121 gccaagtaac ggcgcagatg atcgccaaaa acttgaatat cgcgccgacg ctggaaatcc
    15181 gtccgggcta tcgcttcaac gtcattgtca cgaaagacat gacgttttct aagccctacc
    15241 aggcgtttga ctattaactc caaggagtaa cttatgaaga agctcgctaa gaatgtttta
    15301 gccgctaaag tagctctggt gctggccctc tcggtcggca ccttggcggt cacgcctgcg
    15361 caagcgggca ttccggtcat cgacggcacc aacctgtcac aaaccactgt caccgcgatt
    15421 cagcaggttg cgcaggtcca gaagcaaatc gaggaatacc ggacgcagtt gcagcagtac
    15481 gaaaacatgc tgcaaaacac ggtggccccg gccgcctacg tgtgggacca ggcgcagtcc
    15541 accatcaacg gcctgatgag cgccgttgat accctgaact actacaagaa ccaggcgggc
    15601 agcatcgacg cttacctggg caagttcaag gacgtgtcct actacaaggg gtcgccgtgc
    15661 ttctccctgt cgggctgctc ggaaagcgag cgcaaggcga tggaagagaa ccgccgcctg
    15721 gcgtccgaat cgcagaaaaa ggccaacgat gcgctgttcc gtggcctcga tcagcagcag
    15781 agcaacctca agtccgacgc cgccacgctg gagcaattga agggcaaggc gacgacggcg
    15841 cagggccagt tggaagccct cggctacgcc aaccagttcg ccagccagca ggccaaccag
    15901 ctcatgcaaa tccgtggcct tctgcttgcg cagcagaacg ccatcgccac gcagatgcag
    15961 gcccagcagg accggcaggc ccagcaggac gctgcgggcg cgaagctgcg cgagggttcg
    16021 taccgcgcaa gcccgtctaa gacctggtga ggggaggcgc gatgaagaaa tccaacttca
    16081 tcgcagttgc cgcgctggcc gccgtcatgg cggccagcct ggcaggctgc gacaacaagc
    16141 ccgacaccga caagctgacc tgcgccgatc tgccgaaggt cacggatgcc gctcaacgcg
    16201 cggagctgtt gaagaagtgc ccgcgcggag aaccgggagg cttcaagccc agcgaaaaga
    16261 aagagtggtg atgacgtatg aaaatccaga ctagagctgc cgcgctcgcg gtcctgatgc
    16321 tggccttgat gccggtagcg gcatacgccc aaatcgacaa ttcgggcatc ctcgacaacg
    16381 tattgcagcg ctaccagaac gccgcgagcg gctgggccac tgtcgtccag aacgccgcaa
    16441 cctggctgtt ctggaccttg accgtgatta gcatggtctg gaccttcggc atgatggcac
    16501 tgcgcaaggc cgacattggc gagttcttcg ccgagttcgt gcggttcacc atcttcaccg
    16561 gcttcttctg gtggctgctg accaacggcc cgaatttcgc gtcgtccatc tatgcgtccc
    16621 tgcggcagat tgcaggccag gcaacggggt tggggcaggg gctttcgccg tccggcatcg
    16681 tcgatgttgg cttcgagatt ttcttcaagg tgatggacga aacctcgtac tggtcgccgg
    16741 tcgatagctt cgtcggtgcc tcgttggcgg ccgccatcct ctgcatcctg gccctggtcg
    16801 gcgtgaatat gcttctgctc ctggcgtccg gatggattct tgcctacggc ggtgtgttct
    16861 tcctgggctt cggcggctcg cgctggacct cggacatggc gatcaactac tacaagaccg
    16921 tcctcggggt cgccgcgcag ctcttcgcaa tggtgctgct cgtaggcatc ggcaagacct
    16981 tcctcgatga ctactacagc cgcatgagcg aaggcatcaa cttcaaggaa cttggagtga
    17041 tgctgatcgt cggcctgatc ctgctcgttc tggtcaacaa ggtgccgcag ctcatcgccg
    17101 gcatcatcac cggcgcgagc gtcggcggtg ctggtatcgg ccagttcggc gctggcacgc
    17161 tcgtcggtgc ggccgcgacg gccggcgcgg caatcgcaac tggcggcgca tctatcgcgg
    17221 ccggcgctgc ggcggcggcc ggtggcgcgc aggccatcat ggcggccgcg tcgaaggcca
    17281 gcgataacgt ctctgccggc actgacattc tgtcgagcat gatgggcggc ggcggtggcg
    17341 gcggcggtgg tagcgccggc accagcggcg gcgacggcgg cggctcgggt ggcggcggtg
    17401 gctcgggcgg cggtgaaacc ccgatggcct cggccgccgg cgacaacagc agcggcgcac
    17461 gcggcggcag ttcgggcggc ggctcgggtg gtggccgttc gtctggcggt atcggtgcca
    17521 cggcggccaa gggcggccgg atcgcggccg ataccgtcgc caacctggcg aaaggtgccg
    17581 gctcgattgc caaggccaag gccggcgaaa tgcgcgcatc ggcccaggaa cgcatcggcg
    17641 ataccgtagg cggcaagatc gcgcaggcaa ttcgcggcgc gggtgcggcg gcgcagaccg
    17701 ctgcaaccgt cgccgatagc aacagccagg cgcaggaaca acctgcaccg gcacccgcac
    17761 cgtcgttcga cgacaacagc ctttccgcaa gcaacaacag ggaagcggcc gccgacgcgg
    17821 attccgaagt ggcgagcttc gtcaacaagc ccgcccaatc ctgaaacgac tcttaggagc
    17881 tacgaccatg caactgaaaa aagcgttctc gtcggccgcc ctggtggtgg ccttgggcct
    17941 cggcgcaact ggctcggcca gcgcgcaaga cgtgctgacg ggcgataccc gcctggcctg
    18001 cgaggccatt ctgtgcctgt ccacgggcag ccggcccagc gagtgcagcc cgtcgctctc
    18061 gcggtacttc ggcatccaca agcgcaagct gtcggacacg ctcaaggcgc ggctgaactt
    18121 cctcaacctc tgcccggtat cgaaccagac gccggaaatg cagacgctcg tttcctcgat
    18181 ttcgcgcggg gccgggcgct gcgatgcgtc ctcgctgaac tccgtgctgc gtgagtggcg
    18241 gagctgggac gaccagttct acatcggcaa ccgcctgccg gactactgcg cggcctacac
    18301 cggccatgcc tataccgact tcaacacgac cgcgccgcgc tacgtcggca cgccggaaga
    18361 gggcggctat tggatcgagg cggccgacta cgaccgcgcg ctcaaggagt acgaggcgaa
    18421 gctgaaagag cggcagcagc agtacggtcg ctatggcagc gacgcctacc gtcggttcga
    18481 gcggtaaggg gaggggatag cgatgccgtt tgccaagctg ctggcacgga acgctctgcc
    18541 ggtggtcgcc ctggtggcgg ccactggctt cggtgcggcg gatgcgaccg ccgcacggct
    18601 cttccccgat ctgtcggaac agatggaaga gcgcgttgtg tgctcggtgt ctgcggccgc
    18661 gaagtacgag attccggcca acattcttct cgccattcgg gaaaaggagg gcggcaagcc
    18721 gggccagtgg gtcaagaaca ccaatggcac ctatgacgtg ggcgagctgc aattcaacac
    18781 cgcctacctg ggcgacctgg cgaagtatgg gatcacggcc caggacgttg ctgcggcagg
    18841 ctgctatccc tatgacctgg cggcctggcg gttgcgcggg cacattcgca acgacagggg
    18901 cgatctgtgg acacgcgccg ctaactatca ctcgcgcacg ccgtcgaaga acgcgatcta
    18961 tcgcgccgat ctgatggtga aggccgacaa gtgggcgaag tggctggatg cgcgtttcgt
    19021 caccgtcaac tatggcccca gctcgccggc gcagccggca gggaagggga ccacacttgc
    19081 ggccgctgat acgtcggcag cagcgccggc cgaagcgcag ccgatgaagc aaggccggat
    19141 cacccgcacc agcctccgca gctcgggtta cgtaccccgg cagctcatca tcaacaacac
    19201 gccataagga ggaacggccg tttagcggct aaagcctatg ggcattcgca acctgacgca
    19261 gcgatacatg aacggggcca gggcctacgc ggcctgggcg gcatcgcagg cgaaagcgcc
    19321 gtttgatctt ctggtactgg gcatcgggcc tgtcatcgtc tttggcctgg tcgcgcatac
    19381 gctgctcgcg ttcctgccca catgggccat gtacgccgcc ggcgctctgc tggtcctcgc
    19441 ggccctgcct ttggcgctgc acgtcctccg ggaatacgcg ctgcgctatg ggcgcaaata
    19501 gcgccctgca gggcgttctt actccaaggg ggagggcatg aatacacgcg ccatgaacga
    19561 cgccagcggc cgggcctcgc tgcctgccat ggtgatcgcc gacggcacca ttgaagcctt
    19621 gaagtggctc gccttgcttg ccatgaccgg ggatcacgtc aacaagtacc tgttcaacgg
    19681 tacgctgcca tatctgttcg aggcggggcg cttggccctg cctcttttcg ttttcgtcct
    19741 ggcgtacaac ctcgcccgcc cgggcgcgct cgagcgcggt ttgtacgggc gagcgatgaa
    19801 acgcctgttg gccttcggcc tggtcgcctc ggtcccgttc attgcgttgg gtggagtggt
    19861 gggcggatgg tggccgctga acgtcatgtt cacgctgttg gccgcaaccg cgatgctcta
    19921 cctggtcgag cgcggccgct cggtcgctcc tatagcgctg ttcgtcgtgg ccggcggcct
    19981 ggtcgagttc tgttggccgg cgctgctgct ggccgcgtct gtctggttgt acctcaagcg
    20041 cccgacgtgg gcggccgcgt tgatggcgct gctgtcttgc gcgtccctgt ggtacatcaa
    20101 tggcaacctt tgggcgcttg ctgttgtgcc cctggtgatc gtcgccgccg gcgtcgatct
    20161 tcgtgtcccg cgcctgcgct gggcctttta cacgtactac ccgctgcatc ttgccgctct
    20221 ttggctgatc cgcattccga tgcgcgaggc gggctacttg tttttcacct gacctttgag
    20281 attccaatat gcaattgctc aagaaatgca ccatcgcggc cctgccgctg ctcgccctgt
    20341 ccggctgcgc actgctgaac atccccatgc cgacgccgcc cggttcgacc ccgccggaaa
    20401 tgctgaccgt gccagtggcg caaatctgcc gcgacgctga caagaaccct gttcgggcaa
    20461 cggagctgta cggcaagaaa gggttgtcgg ccaccggcaa ggtgcaggtg atttccgaag
    20521 gcttcaagcc tcgctatcgg gtgctgctgc gcgctggcag cgcctcggtc catgctggga
    20581 ccgataacca gctcgccatc aagtcggttt ccaccggcca gaccacgcgc gtcactggca
    20641 ccgtgaagga cgtgtcctac gaccataacg gctgctcgat ctcgcttgac gatgcgaagt
    20701 tctactgagg ggagggcggc ggatgctgac acggttgaag ggcttccttg ctcgtcgccg
    20761 cgagttgaag gaactggatg tgtccgtggt gagccggccc cggccggctc cggcggaatt
    20821 ggtccaggtt gatgcacgcg aggccgtttg gcgcgtgccg gtgcccggcc aggccgaccg
    20881 cttcatgtcg gccaagcctg gcgcgatcaa cgatgaaatg ttcgtggttc gggtggacac
    20941 cgaagcgttc tatcgggctt ggctgcgcag cagctcgacg ggccgcgaaa cgcggtcgga
    21001 caactgcccg ctgcgctcgg aaatgccgca ggactacaag ttcaagcacg ccgtccaggg
    21061 cttcgcgcac ggcagggaaa atcctgtgcc gctggccttc gccggcgcgc accaggagcg
    21121 ccaccgggtg gacattggtt tcagcaacgg ggtcacgcgc tcgttctggc tgattgccaa
    21181 caaggctccg tcgttcccga tccaggtcca cggccgggag tcggccgagc tgctgaacaa
    21241 ggtttgcggc ctcgatcctg cgccgctgtc gttcacggaa ctgttcgcgc aggcccaacg
    21301 ccaggctccg caggtcgcca caccggcccg gcctgcgccg gcagcggcca cccggccagc
    21361 tcccaaggtg cagccacgcc ccggccgaag cggcccgcgc aaaggccgcg gactctgact
    21421 acaaccgtgc gcaaggcgca ttagggagga tgtatgtatg taatcgcctg cggcatcgtt
    21481 gccggcttgg cggctgcggt ggccctgttg ggcttcacgc cgatgatgga ggcgcttgcc
    21541 gccggcgaac gccgcaaggc actcgcgcaa tggacgcgga cgatgttcct ggtgctgctg
    21601 cctgtcgtgc tgatgtgcgc gcccatcggg tccagcattt acgacgccgt gcaagcggac
    21661 gctggcaagc ccatcgcttt ccacaacggc cggatcacgg tcgtcatggc cctggtcggc
    21721 agcttggccg ttgtcctggt cgcggctgcg cgtgcggtgg tcaaccgcaa gcatgccagc
    21781 ttctggttcg tcggctgggt gatggcgtcg gttttggccg gcggcgtcgg cgcgatcgcc
    21841 agcgcgaagc aactggcgtt cctcggcgaa catagcggca tggtggcctt cggcttcttc
    21901 cgcgaccagg tgaaggacat gcactgcgat gcggacgtga tcctggcccg gtgggatgaa
    21961 aaggcgaact cgccggtggt ctaccgctgc ccgaaggcgt acctgctcaa caggttcgca
    22021 tccgcgccct tcgtgccctg gccggactac accgaggggg aaagcgagga tctaggtagg
    22081 gcgctcgcag cggccctgcg ggacgcgaaa aggtgagaaa agccgggcac tgcccggctt
    22141 tatttttgct gctgcgcgtt ccaggccgcc cacactcgtt tgacctggct cgggctgcat
    22201 ccgaccagct tggccgtctt ggcaatgctc gatccgccgg agcgaagcgt gatgatgcgg
    22261 tcgtgcatgc cggcgtcacg tttgcggccg gtgtagcggc cggcggcctt cgccaactgg
    22321 acaccctgac gttgacgctc gcgccgatcc tcgtagtcgt cgcgggccat ctgcaaggcg
    22381 agcttcaaaa gcatgtcctg gacggattcc agaacgattt tcgccactcc gttcgcctcg
    22441 gcggccagct ccgacaggtc caccacgcca ggcacggcca gcttggcccc tttggcccgg
    22501 atcgacgcaa ccaggcgctc ggcctcggcc aacggcaagc ggctgatgcg gtcgatcttc
    22561 tccgcaacga cgacttcacc aggttgcagg tccgcgatca tgcgcagcag ctcgggccgg
    22621 tcggcgcgtg cgccggacgc cttctcgcgg tagatgccgg cgacgtagta cccggcggcc
    22681 cgcgtggccg ctacaaggct ctcctggcgt tcaagattct gctcgtccgt actggcgcgc
    22741 aggtagatgc gggcgacctt caaccttcgt ccctccggtt gttgctctcg cgtcgccatt
    22801 tccacggctc gacggcgtgc ggatcggacc agaggccgac gcgcttgcct cgcgcctcct
    22861 gttcgagccg cagcatttca gggtcggccg cgcggccgtg gaagcgatag gcccacgcca
    22921 tgccctggtg aaccatcgcg gcgttgacgt tgcgcggctg cggcggccgg ctggccagct
    22981 ccatgttgac ccacacggtg cccagcgtgc ggccgtaacg gtcggtgtcc ttctcgtcga
    23041 ccaggacgtg ccggcggaac accatgccgg ccagcgcctg gcgcgcacgt tcgccgaagg
    23101 cttgccgctt ttccggcgcg tcaatgtcca ccaggcgcac gcgcaccggc tgcttgtcta
    23161 ccagcacgtc gatggtgtcg ccgtcgatga tgcgcacgac ctcgccgcgc agctcggccc
    23221 atgccggcga ggcaacgacc aggacggcca gcgcggcagc ggcgcgcagc atggcgtagc
    23281 ttcggcgctt catgcgtggc cccattgctg atgatcgggg tacgccaggt gcagcactgc
    23341 atcgaaattg gccttgcagt agccgtccag cgccacccgc gagccgaacg ccggcgaaag
    23401 gtactcgacc aggccgggcc ggtcgcggac ctcgcgcccc aggacgtgga tgcgccggcc
    23461 gcgtgtgccg tcgggtccag gcacgaaggc cagcgcctcg atgttgaagt cgatggatag
    23521 aagttgtcgg tagtgcttgg ccgccctcat cgcgtccccc ttggtcaaat tgggtatacc
    23581 catttgggcc tagtctagcc ggcatggcgc attacagcaa tacgcaattt aaatgcgcct
    23641 agcgcatttt cccgacctta atgcgcctcg cgctgtagcc tcacgcccac atatgtgcta
    23701 atgtggttac gtgtatttta tggaggttat ccaatgagcc gcctgacaat cgacatgacg
    23761 gaccagcagc accagagcct gaaagccctg gccgccttgc agggcaagac cattaagcaa
    23821 tacgccctcg aacgtctgtt ccccggtgac gctgatgccg atcaggcatg gcaggaactg
    23881 aaaaccatgc tggggaaccg catcaacgat gggcttgccg gcaaggtgtc caccaagagc
    23941 gtcggcgaaa ttcttgatga agaactcagc ggggatcgcg cttgacggcc tacatcctca
    24001 cggctgaggc cgaagccgat ctacgcggca tcatccgcta cacgcgccgg gagtggggcg
    24061 cggcgcaggt gcgccgctat atcgctaagc tggaacaggg catagccagg cttgccgccg
    24121 gcgaaggccc gtttaaggac atgagcgaac tctttcccgc gctgcggatg gcccgctgcg
    24181 aacaccacta cgttttttgc ctgccgcgtg cgggcgaacc cgcgttggtc gtggcgatcc
    24241 tgcatgagcg catggacctc atgacgcgac ttgccgacag gctcaagggc tgatttcagc
    24301 cgctaaaaat cgcgccactc acaacgtcct gatggcgtac ttacccaaag aacagctagg
    24361 agaatcattt atgctcagca cacttccaca agctcatgca actttcttga accgcatccg
    24421 cgatgcggtc gcttccgatg ttcgcttccg cgctcttctg atcggcggct cttacgttca
    24481 cggaggactc gatgagcact ccgatttgga tttcgacatc gttgttgagg acaactgcta
    24541 cgcagatgtc ttgtctacac gcaaggattt tgccgaggca ctgcccggct tcctcaacgc
    24601 gttcaccggc gaacatgtag gagaaccgcg ccttctgatc tgcctatatg gtccgccact
    24661 gctacacatc gatttgaagt tttctcttgc ttccgatctc gaccagcaaa tcgagcggcg
    24721 ggcggttctg tttgctcgtg atccggcaga gatcgagaag cgcattgagg cggcagcggt
    24781 ggcatggcca aaccgtccct ccgagtggtt cgaagcacgt tgtcagcgcc agtgatataa
    24841 gacggtaatt caccatttgg attgtccgct ccacccaaca tgttgtttcc ttaaggttct
    24901 cacaccagaa aggacatcaa catgctgagc agagaggact tttacatgat aaagcaaatg
    24961 cgccagcagg gcgcgtacat tgtcgatatt gcgactcaga ttggttgctc tgaacggacg
    25021 gtcagacgct acctcaaata ccctgaaccg ccagccagaa agacccgcca caaaatggtt
    25081 aagctgaaac cgtttatgga ttacatcgac atgcgcctgg cagagaatgt ctggaatagt
    25141 gaggttatct ttgcggagat taaggcaatg ggttatacgg gcggacgttc catgctgcgt
    25201 tactacatcc agcccaaacg taaaatgcgt ccgtcaaaaa gaacagttcg cttcgaaact
    25261 cagcctggat accagctcca gcacgactgg ggcgaagttg aggtggaggt tgccgggcaa
    25321 cggtgcaaag ttggcgcgcc cttggcagaa catatccatc gcgtccgcca tctccagcag
    25381 ccgcacgcgg cgcatcgggg gggggggggg ggtttcaatt catcattttt tttttattct
    25441 tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa
    25501 gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa
    25561 acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga
    25621 agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
    25681 gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat
    25741 gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta
    25801 ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta
    25861 aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt
    25921 ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac
    25981 attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg
    26041 gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc
    26101 tccctagcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat
    26161 tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg
    26221 ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat
    26281 agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta
    26341 tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa
    26401 gcatatttga gaagatgctg ccagcaaaac taaaaaactg tattataagt aaatgcatgt
    26461 atactaaact cacaaattag agcttcaatt taattatatc agttattacc cggccgggaa
    26521 tctcggtcgt aatgattttt ataatgacga aaaaaaaaaa aattggaaag aaaacccccc
    26581 cccccgcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggcg
    26641 cgccaacttt gcggttaata cgctggggtt ctcccgccgc ttccatgtct tcgccgcacc
    26701 aaaacaggat gctgagcata cctacgaatc actggttcgc gccttccgct acttcggtgg
    26761 ttgtgtgaaa acggtgctgg ttgataacca gaaggctgcg gtgctgaaga ataacaacgg
    26821 gaaagtcgtg ttcaactccg gattcctgtt gctggccgac cactataact tcctgccacg
    26881 ggcatgccgt ccacgcaggg ccagaacaaa aggtaaggtt gagcggatgg tgaaatacct
    26941 caaggagaac ttcttcgttc ggtaccgcag gttcgacagc ttcactcatg ttaatcaaca
    27001 actggagcaa tggatagccg atgtggctga caaacgggaa cttcgccagt tcaaagaaac
    27061 gccggaacag cgcttcgcgc tggagcagga acatctgcag ccgttaccgg atacggactt
    27121 cgataccagt tacttcgaca tccgccatgt gtcctgggac agctatatcg aggttggtgg
    27181 taatcgttac agcgttcccg aagcgctgtg tggtcagccg gtatcgatac gaatatcgct
    27241 ggatgacgag ttgcggatct acagtaatga gaaactggtg gcctcacatc gcctctgttc
    27301 ggcatcgtct ggctggcaga cagtgccgga gcatcacgcc ccgctctggc agcaggtcag
    27361 tcaggtggaa catcgaccac tgagtgccta tgaggagctg ttgtgatgca tgagctggaa
    27421 gtcctgctga gtcgcctgaa aatggagcat ctgagttatc acgttgaaag cctgctggaa
    27481 caggcagcta aaaaagagct gaactaccgg gagttcctgt gcatggcgct acagcaggaa
    27541 tggaacggca ggcatcagcg cggtatggag tccaggctga agcaggctcg tctgccgtgg
    27601 gtcaaaacgc tggagcagtt cgactttacc ttccagccgg gcatcgaccg taaggttgtc
    27661 cgggaactgg ctggtctggc gttcgtggag cgcagcgaaa acgtgatcct gctgggacct
    27721 cctggtgtcg gaaaaactca tctggccata gctcttggcg tgaaagcggt ggatgcggga
    27781 catcgggtac tgtttatgcc actggacaga ctgatcgcga cactgatgaa agcgaaacag
    27841 gaaaaccggc tggagcgtca gctgcagcaa ctgagttatg cccgggtgtt gatcctggat
    27901 gaaataggct atctgccgat gaacagagag gaagccagcc tgttcttccg gctactgaac
    27961 cgtcgatatg aaaaagcgag catcatactg acgtcaaaca aagggttcgc agactgggga
    28021 gaaatgttcg gagatcacgt gctggcaaca gcgatactgg atcggttgct acatcactca
    28081 accacgctga atatcaaagg agagagttac cggttaaaag agaaacgtaa agctggagtg
    28141 ctgaccaaaa acacaacgcc aatcagtgat gatgaaatgg tgaaaagcgg acagcatcag
    28201 taacgaaagt atcttagcgg gcatgaaaat ggcaaataac ggtcaaacat cgtggcgttg
    28261 acaacgtgcc tggatctggc tacactatgc ggccaccaag ctcgcccgtg gcgagcttta
    28321 cgaagcgatc ggcatgctcg gtttcttccg tgagcaagtg ttaggacctt tgctctaccg
    28381 tcgcgctgga aaggaccagc gcggagtgag gcgattggaa acccttcgac tggatgaaga
    28441 gcgcagacta gccaccacca ttgcgctgca cgatgcgttg tctgtcaggg atgccatcaa
    28501 agcatctgcc tccatctatc tcgacctccg agccgccgat ccgtcgttgg aaccgacaac
    28561 gcatatgcca ggtcttctgt acgacttaat agaacgtgcg gtaccaggca cgcctaaccg
    28621 tcagtgagat tggatgagtg aacgatattg atcgagaaga gccctgcgca gccgctgccg
    28681 tgcccgagag catggcggct cacgtgatgg gatacaaatg ggcgcgtgat aaggttggtc
    28741 agtccggctg cgcggtctat cggctgcata gcaagaattc tgccgtgtta tggaactgtc
    28801 ttgaggagtt cgaaccttcg cttcaggaga ggcttgttgc gcaatatggc attgccgatc
    28861 cggataggcg caagctgcaa tttcatctcc tgctggacga acttttctaa ggcgatgccc
    28921 cctcgacctc gatcagggag gcgttcagga cgactcacaa agaaagccgg gcaatgcccg
    28981 gctttttctg ctgctacctc cgtagtcgta aggtcgttgc aggtgctcgg gtgcggtaca
    29041 actcgccggt cgccagctca agcgcgatca cgtcgttgcc gtcgtagttg acgatgatgc
    29101 tgttgggccg actgtcctca cgcttcgcag ggagaggcca gccttcaatc gaagccggcg
    29161 caagctcgta gtgcttcccg gtttcgacgc tgcgcagcgt ccaggtcctg caaccggcca
    29221 cgccggtcgc agaaaccacg gcgagcgagc cgcgaaaatc gtgcgggtac gcctcgatgt
    29281 tcatacgcct cctagatcga gcgcgagcgt ttctgctcgg ccttggccgc ctgttcctgg
    29341 gacacctcgc cgatgacctt gccctggccc cggctgtagg cgatttcgta gttcttgccg
    29401 acaaccggcg gcttctcaaa gatgccccgg ctgtgtttca cgatcccgcc ttcgctgaac
    29461 tggtagacgt tgcgcccatc gtcgtgcagc acctggccga cgtgcttgtg cgggtggacg
    29521 tttttgcttg cgtccttcgc atcgctcaac tggtgaatgc ctttcggtag ccccgcctcg
    29581 ggcaacacct tcatggtcag ccattcgccg ttcactacct ggtccacctg gcggctgccg
    29641 ttcatgacgg cgatcttgac gctgccctcg ggtttcatga tgacgccagg gcttgccgat
    29701 gtgcgtggtg ccccgatctg tactttgttc atacgctcta gttctcctta gtaggttctc
    29761 gcgcggcgtt gccgctgttc ttgctgctcg atgtcttgct gcttgagctg ctgcaccttc
    29821 tgccgctggc cctcgtcgag aagcaccttg ccgacagcac ttctcacctg gcgttcaacc
    29881 ccgtccttgc ccaggctgct gcgctcggac aggtcgttga aatcggtgtg cttcttcatg
    29941 ttcgacaggg cggcgagctg gccatcgttc aacagcgatt ccttgagctt ggccgtgtcg
    30001 gcctcgctca actggacctt gccggccgcc gcgtccgcga ggcgcttttc agcgtgcaaa
    30061 tggttgcggt agttctccgg ggtgatcggc ggcagctcct tcgggtaggc gttctcgccc
    30121 ggcgcgaaga ttgggaagat ggccttgccg ccgaccgcct tggcggcctc ctgtgccttc
    30181 gtcctgccgg gattcacgcc ctgggtgatc tgcacctggc ggtcgtcgtc gccggcgatc
    30241 acaacgggct tgtccgggaa tttcgcgtgc agggcctcgg caacagcctg taggttgccg
    30301 gaatcgaacg cggcgacagt cgcgtgcccc agcgcttcgg ccactgtggc ggcggtggca
    30361 tagccttcgc cgatcaccag cgccggcgcg gccgcgagcg catccatgcc accgacgaca
    30421 tggaagcatc cttccttgcg gctgtccttg gcgaagcgct tggtgccgtc ctcctggatg
    30481 tactgcatgg tccattgctt gccgtcggcg tcgtaggccg ggatgtaggt tttctggccc
    30541 tcctggtcgg taaggacgcc ggcgtgcacc tgtagaccct tgtcgcgcag gtacggcgtc
    30601 ggttccgtga tgggaaccag gctttgcgcc tggcggccga tgcgctgcgc cgtggcttcg
    30661 tgctggcgtt cttgttcctc ggcacgcgcg gccagcttgg ccgccgcctc ggcctgcatc
    30721 ttggccttct cggcggggtc cagggcgtag cccttggcct tccacttcat ttcgacgccg
    30781 gtgcggttgt ttttgatgta accggccggg tggccgtcga ggtggccgac gtagaagccc
    30841 gacttctcgc ccttcttgtc gccctcggtc tcgatgcggt gcttcttgcc gtccatgatg
    30901 gggtgctcgc cgcctggggt gacgacgcag cccatgcttt tcagggcctc cgcgaactca
    30961 tcttcggggg tgacggccgg ggattgctgg gtgggcacgt tgtccggcag ccagcgttgc
    31021 agcttgccca tgtcggcgtt cggtccggcg taccaggact tggccacctt gtcccactgc
    31081 gcgccggccg ccttggcaac ctggcgctcg ccgtagggca cggccaggta gacgcgctcc
    31141 tgggccgcgt tggggcgctc ggccgtgggt tgggtaggct gggcctcggc gcgggcctct
    31201 acggccgctg tagcgccctc gcgcgcccat ttggcgaacg gggcagggtc aacccctgcc
    31261 ggaacgtacc aggcgcgttc ctggcggtcc cagcgcgctc caagggcctt cacctcgtct
    31321 ttctccttga acggcacgtt caagtaggcg cgctcgggct tggcgggggc ttgagcggcc
    31381 gccggctgct cggcggcgtt catggcctgg gccatttcct gctgctcgcg ctcgtagtcg
    31441 gcgatccggc gctgtaggtc ctcgtcgtgc agcatggccg tgccctcggc ggccttgcgc
    31501 gcctccttgg cggcaacgcg gtcctcgtcg gtgctgttgg gatcgcggcg aactcgctct
    31561 tcatggatgc gggcgaactt cgcggcctgc tcgtactcgt tggccgtcgc gtaagcgtcg
    31621 atcacggcca ggcggtcggc gagcgcttcg gcattggtct gcgcgtcggg gccggcgaag
    31681 tcggcaagcc attggtggcc gccccacgca tggttcgcat agacgcccca aaactccggc
    31741 tctcggtcgc cggccggcac cacggaccgt tcgccgtcgt gctcgacctc gacgttggcc
    31801 tggacctgga cgcggccggt ccaatcggca ggcagctcaa agcccagcgt ggtttcggtc
    31861 agcgcggcca gcgattggtt gccttccgcc ggctccgcgc cggcgcggta catgcgcagg
    31921 gtctgcgcga tcagctcgtc ggccggcgca atggccggtc gtgccacctg gtcttgctgt
    31981 tgctccatag ttgccccctg cgcaggctcg atggcctgct gggtcgtttg ttcttgaatt
    32041 tgcttctgct cgaacgccag gacgaaatcc tggatcttct ccgcgtcggc ggccgcgcgg
    32101 aaaatctcta gcgggtcctc ttgtagcgcc ttgatccacg atccgacata ggccgcgtgc
    32161 tggccggggt cgtggccgat gcccagctcg tcgcccagga tcatgctggc aatctcggcc
    32221 cgcagctctt ccttggcgta cccctcgctc ccgaagggat gcgccaggtc gcggtccagc
    32281 cgcgacgggt ggccggtcca gtgccccagc tcatggagcg cggttgcgta gtagttgtcg
    32341 gcgctcggga actggccttt gtcgggcaga tggatgctgt ccgtggacgg ccgataaaac
    32401 gcgcggtcgt gctcgccgtg gcggatggtg gcacctgacg ccgcaaggat gtgctcggcc
    32461 cgctcgacgg cgctccaagt ctgttccttg cgttccaacg gcggcaggcc gtcgatctgc
    32521 tccgcattga acacggtggc gaagaacacg cgcgggcgtt cgagctgcac cgtcaccttg
    32581 accggatcgc cgttggcatc gaggaccggc ttgccggtct gctcgtcggt cttggtctgc
    32641 tcttcgctga acttccaata ctggatcggc gtgcctttct cgccgcgacg cacctgtgcg
    32701 ccggcggcag cggcctgctt gtaggtcatc cagcgcgggt ccgcatggcc ctgggccatg
    32761 agctgaatcg cgttgatgcc cttgtaacgc ttcccggtag tcgggttgag cgggatgaag
    32821 gagccgggca tgcccggttc ccacggtttt tgccacggcg cagtgccggc tttcagttgc
    32881 tcaatgaggc gttcggcaac ctgctcgtgg aacggctttt tgacctctgc catagccaat
    32941 tacctcccgt cattggcggc cgcggtcgtc gtgtcctcgg gcacggtcgc gtccaccagg
    33001 tcaatgtcgc tctcggcggc gtcctgctcg ctcaaggcgt cctcggggaa ggccccggcc
    33061 ttctccgctt cttccgggtc gaactcgacc tggaagccgg gcgtcatcgc atcggcgagc
    33121 ttttcacgca gggcggcggc ggcgttcgcg gtatcgtcga acggcgcaaa gtcgtcctgc
    33181 tgcacggtct tgggatcatt catcgctttc actcctggtt ggtgccgtta cggcctttgc
    33241 tgtagtccgg cctgcctttc aggtcggggt atgtctgctt gcacgtcggg aagttgctgc
    33301 acccccacca gaacatgccg cgcttcttgc caggccgacg ggaaaggccg tggccgcagg
    33361 ccatgcactt gtgcagctcg gagactttcg gggcctcgcg cgggacgggc ttgccgccct
    33421 tgtcgtcgca cgcgaacttg cagccgtcgg caaagccggt gcagccccaa aagtattcgt
    33481 tcttgtcctt cttcttgagg cgtcgcagcg gcttgccgca ggacgggcaa gggtgcgtgt
    33541 cgatcttcat gttgaggccg ttgtctttga tgttggcgac ctcggcgccg atgtattcca
    33601 tcagctcgtt gacgaacgac agcgtgtcgc gctcgccggc ctggatggcc ttctgctgct
    33661 catgccagag cgcggtcatg tcggggaatc tggccgtgtc gggcagtgcg tcgtacagct
    33721 cttcgccggt cggcgtggac acgatgtgct tgcccttctc caccaggtag ccgcgctcga
    33781 aaagcgtggc gatgatggag tctcgcgttg ccggcgtgcc gatcccgccg tgctcgcctt
    33841 gcttgccctt gtccttttcg atcaagattt tccgcaggcg gtcatcgcgg atgtatttcg
    33901 caacgcgggt aaggtccgac agcagagatt ccatcgtgta cagcggctgc ggtttcgtct
    33961 cctgctgctc ggccttcgca tcggtgcagg tgccggcctg gccgtcacgc agcttgcgca
    34021 ggtcctgttc aatgtcgtcg gcattgcctt ccaggtcctc gttgccggcg tcgttcttgt
    34081 agagaatctt ccagcccggc gacgtggtga cgttcgagcg cacgccgaaa cgatgatcgc
    34141 cgacctgggc aagcacgtcg gtctggtcat acagatgctt cggccagaac tgcgcgacgt
    34201 aggcgcgcgc gatcagcagg taaatcttct gctcggcatc ggtgagcttc gacaggtcgg
    34261 ccgtgctttc ggtcgggatg atcgcgtggt gcgcggaaac cttggacgag ttgaaggcgc
    34321 ggctcttgat cgtcggattg gcgcgctgcg cagcagcggc cagcatgggg gccgtctgtg
    34381 cgatggccgc cagcacgccc ggcgcatcgc cgtgctgttc ctcgctcaag tattcgcagt
    34441 cggaacggtt gtaggtgatg agcttgtgct tctcgcgcag ggcctgcgta atgtccttca
    34501 cctggtccgg cttgaagccg aacttgcgcg aggcgtccat ttgcagtttc agcaggttgt
    34561 agggcagcgg cgcagccgct tccttcgcct tggtggtcac ggacacgatg cgggcgggtt
    34621 ggccgctcac ggcggccgcg atgccctcgg cgtgctcctt gttgctgagg cggcctttct
    34681 cgtccaccgg atcgccgtcg gcgacctggt aacgggccgg gaactgaatg ccctcgacct
    34741 cgaactggcc gttcaccagg tagtagtagg ttttctggtg ggccgcgttc tcgcggcaac
    34801 ggcgcacgac aaggcccagg atcggagtct gcacgcgccc cacgctcaac agcccctgat
    34861 agcccttcgc gcgtgccgca agcgtgtaca ggcgcgtgat gttgaagccg tatagctggt
    34921 cgccgacgct gcgggcctcg gccgcagcgg acaggccggc gaactcgcgg ttgtcgcgca
    34981 tcgcggcgag ctgccggcgc acgatcttca cgttgttgtc gttgataagc agccgctgca
    35041 ccggcagacg gcagttggcg tattccagga tttcatcgac cagaagctgg ccttcgtcgt
    35101 ccgggtcgcc ggcgtgaacc acgcttttcg cctgcttcaa caggctgagg atggtcttga
    35161 actgagcttt cgcaccggca tcgccggacg gtttcttgcg ccagggaata tggacgatgg
    35221 gcaggtcggc catgttccag ttggcgtagc gctcgtcgta gtcctccggg tctagcaagg
    35281 ccagcatgtg accgtagcac caggtcacgc ggtcggagcc gcattcgtaa tagccgtcct
    35341 tgcggctgcc gccgcccagg ccctcgacga tggcttttgc cagctccggt ttttcagcga
    35401 ttacaaggcg ttcaaattgc atatatcccc ctaccctcac caggtcagaa ccggcctgat
    35461 gacggtgatg atttgcgaac gattgacagg cccgaagtag cggccgtcga aagacgtgtc
    35521 gcttacgtcg gacataagca gaacctcggc ggtccccagg gtgtagctgt cggactgata
    35581 acgaggcagc ggccgtcctg atggatcggc cttgatgagc gcgctgtgag gcagcagccc
    35641 gccattcacg cgcacgccgg cgtcggtgat ggcaacctcg tcgcctttag cggctaaaac
    35701 tcgcttcatc atgtagccgt agtcgccggg gcagaaaccg ccggcgatgt agccccgctc
    35761 cttggcgtcc gaaaacacgc cgacttgcgg cgggcagaac atgacgtaag cccccttctc
    35821 caccggcgca ttcgatttcc agtacaggcc gaccggaatg cttttggtgg tgttgacctt
    35881 cgcgccggcg agataggccg cgccggcgag caacaaggcc gcgccgcctc cgatggcgac
    35941 gtacttggtg aggcgctgga agcggctcat atcgtgatcc cctccccttc ctcgacggtg
    36001 gccgtctgga tcagcttgtc gctgaccttc ggagccggta cggccgcgcg ggcctggaat
    36061 atcgggtctt tgaagtagag cggctgcttg ccgtagatcg cgggatagcc ggcgacgtac
    36121 acaaccatgt cgcccgcctc ttcaatgctg ccgtcggcgc tcttcttcgg ccccggcatg
    36181 cgcaggcatt catcgggggt cagcaatggc cgctgcactt cctggaaggt ccgcgagacg
    36241 ttgcccaaca gcgccgacgt gcggcggccg ctcgtcgtga tctgctcctt cacgatggtc
    36301 gtggtgcctg tcagttttga caggtgctcg gccgtctcca cgcggttcgg cgggtaggcg
    36361 ttctgcacgt ggcagttcga cgtgatgctt tcgtcgtggc cgtagccggt ttcgcggctc
    36421 ttgagctggt taatgtcctg gcagatgagg tagcacttga tgccgtagcc ggcgacgaag
    36481 gcaagggact cttgcaggat ttcgagcttg cccaggctgg ggaactcgtc gagcatcatc
    36541 agcagacgat gcttgtagtg cgcgacagga cggccgttct cgaagtccat cttgtcggcc
    36601 agcagccgga cgatcatgtt gaccatgacg cgcaccagag gccgcagacg ggccttgtcg
    36661 ttgggctgcg tcacgatgaa caggcttacc gggtcgtcgt ggtgcatcag ttgcttgatg
    36721 cggaagtcgg acttgctgac gttgcgggcc acaaccgggt cgcggtacag ggccaggtag
    36781 gacttggcgg tggacagcac ggaaccggat tcttcctccg ggcggtccat catgtcgcgg
    36841 gccgcagagc cgaccgcagg gtggttctgc ccgtcaacgt ggccgtaggt ggtcatttcc
    36901 atccaaagct cgcccacgtc gcggttcggg tcggcaagca tgccgtccac cgacggcagg
    36961 gtggccggcg taccctcgtt cttagccttg tagagcgcgt gcaggatgac gccgacaagc
    37021 agcgcctggc tggttttctg ccagtgcgat tccaggccct tgccgtccgg atcgacgatc
    37081 agggtggcaa ggttctgcac gtcgccaacc tcgtactcgg tccccaagcg gatttcatcg
    37141 agcgggttcc agcacgcgct accctgcgcg gatgccggct caaagcgcac gaccttgttg
    37201 cgggcatgct tcttccgcca gccggcggtc agcgcccaca actcgccttt caggtcggtg
    37261 atgacggcgc tgtgcgccca ggaaagcagc gtcggaacga ccaggccgac gcccttgccg
    37321 gagcgcgtcg gcgcgtaggt caagacgtgc tcggggccgt tgtgccgcag gtagtggaac
    37381 ttgccgtcct tgtcctgcca gccgcccaca tagacgccgc tggaagtggg cgggtgtttg
    37441 cctgacacca gctcgacgac ggtgcgcggc cggggcagca ggccggcggc ctgtatgtcc
    37501 ttcttgtcgg cccagcgggc cgaaccgtgc agatagtcgt tcgccttgcc ggtgttcgcc
    37561 ttgaccatct gcgtgacggc cgtgcccagc aggcccacgg tcgaaacgac catacccatg
    37621 ctggccgcgc gcatgaaatc gtcgggatat tggccgtacc acttgccggc ccattgaagg
    37681 atcgaccagg gcgtgtagac gtggttgata ttccagccaa gtccggcctg atactggaag
    37741 gaatgggcga aatattgcgt cgcggtctgc aagcctgccc caagggacag gccggcgagg
    37801 atgggaacgg tcttgctggc cttcggtttt ttcgcccgta tctgtggccc cacggcgttg
    37861 tttcggttct tcatctactc ctacctcggg tagttttaag ggagcctcgc ggggtcacgg
    37921 tgacgggatc accgatggcg aggcgcttca tgcgttgcac cgtggcctta tcgacgggca
    37981 gcaccagaat ctcgtcgttt tctttcctca acagggccag cgcctggtcc tcgacgttcc
    38041 gggtgcctgc ataggacagc gcaccaacat aatcagtata tcgtgcatgc ttcggtatat
    38101 cgaagccgtt tagccgcttt tgctcgcgct cggcaacata tttctcggcc gccgcgatct
    38161 gttcgggctt tagccctctt cctggcccag aaactccccg tcgcagtgcg tgagctggtt
    38221 cggctccttg ctgctccacg tgaccaggaa catcacgcgg caatagcatt tcagctccgc
    38281 cggcgatgcg aaccacaccg agttgggaca gcgctcgcaa acggttttgg ctttggggcg
    38341 gcggctttcg tccaatgcgt ccaacgttgg gcttgcggag tgcgacggtt ccgccggcgc
    38401 tgacggcgcg agcgtcccgt cggtcgccgt cgccgcctgt ggcgttgagg gtggttctgg
    38461 ctgcggcagg tcgaatgcct ccatcgccgc cgcgatctct tcgtccgtca tttcgttcgg
    38521 gttgctcatg tgcttgctcc ttcgtcagta gttcttgacg gcggcgctca agggcggcgt
    38581 cgtcaaaggt gattgccaga cggccagcgg cggccgcctg cgcgatccgc tccttgaact
    38641 ctgctgtgcc gttgacggtg atccggtcgc cgaagcgctc cattgccagg cgcagggcgg
    38701 cgtccaggcc gtccgtggtg gcctcgcgcg agacttgcag gcggtcgccg tcgtcgcgga
    38761 cggcgctgct gccgacgcga tagatgatgg ttcccttctt cgtgatgttg tccgtcacgg
    38821 ccgcatggcc cggcttggcc tcgccgctgc cctggatggt gttgcccttg aggtcgctgc
    38881 ggccctcgcg tgcgcgcagc gcggccaggg ccttgtcgtc gcccttcatc gcctcggcct
    38941 tgagccagtc ggcccacgcg cggcgctgcg tgcgctcctg gaccgcctga cggccctgcc
    39001 ggtactcgcg gttgatcttg tccgggtcgg cgcgcagagc cttgtgcgcc tgcgcgtaca
    39061 tcagtcgctt tgcaatgcgc ccctcgccca gcagcttgat agcggcgcgg cgcagccggt
    39121 tgctgcgcat cgcggcttca atcaggcggt cacgacgccg gcgcagcgtg tccagctcgc
    39181 ccttgcgcac ggcccccatt tcctggcgtt cagactgata ccgggcgtat agctcggtgg
    39241 tgtcgatgcg ggtcttgagc ggcttcgctc gatactcccg ccgccggggg gcttcgccgc
    39301 cctcggctgg cgtgaatgcc ccgaatcggg cttcgagctt cggcttggac aggtcgcgcg
    39361 aaacggtgct ggccttgacc gtcgtgccgt cgccggcctc gaagatgaag ccgtttccgc
    39421 gctcgcgcag cttaagcccg ttttcccgca ggacgcggtg caggtcctcc caggattgcg
    39481 ccgcttgcag ctccggcagg cattcgcgct tgatccagcc gaccaggctt tccacgcccg
    39541 cgtgccgctc catgtcgttc gcgcggttct cggaaacgcg ctgccgcgtt tcgtgattgt
    39601 cacgctcaag cccgtagtcc cgttcgagcg tcgcgcagag gtcagcgagg gcgcggtagg
    39661 cccgatacgg ctcatggatg gtgtttcggg tcgggtgaat cttgttgatg gcgatatgga
    39721 tgtgcaggtt gtcggtgtcg tgatgcacgg cactgacgcg ctgatgctcg gcgaagccaa
    39781 gcccagcgca gatgcggtcc tcaatcgcgc gcaacgtctc cgcgtcgggc ttctctcccg
    39841 cgcggaagct aaccagcagg tgataggtct tgtcggcctc ggaacgggtg ttgccgtgct
    39901 gggtcgccat cacctcggcc atgacagcgg gcagggtgtt tgcctcgcag ttcgtgacgc
    39961 gcacgtgacc caggcgctcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg
    40021 cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc
    40081 ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg
    40141 accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca
    40201 tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg
    40261 cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc
    40321 gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc
    40381 gccgcctttt cctcaatctc tcttcgttcg tctggaaggc agtacacctt gataggtggg
    40441 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccgaattc tgacgccgtt
    40501 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
    40561 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
    40621 agcgctgctt ccctgctgtt ttgtggaata tctaccgact ggaaacaggc aaatgcagga
    40681 aattactgaa ctgaggggac aggcgagaga cgatgccaaa gagctacacc gacgagctgg
    40741 ccgagtgggt tgaatcccgc gcggccaaga agcgccggcg tgatgaggct gcggttgcgt
    40801 tcctggcggt gagggcggat gtcgaggcgg cgttagcgtc cggctatgcg ctcgtcacca
    40861 tttgggagca catgcgggaa acggggaagg tcaagttctc ctacgagacg ttccgctcgc
    40921 acgccaggcg gcacatcaag gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg
    40981 cggaacccgc gccggcaccc aagacgccgg agccacggcg gccgaagcag gggggcaagg
    41041 ctgaaaagcc ggcccccgct gcggccccga ccggcttcac cttcaaccca acaccggaca
    41101 aaaaggatct actgtaatgg cgaaaattca catggttttg cagggcaagg gcggggtcgg
    41161 caagtcggcc atcgccgcga tcattgcgca gtacaagatg gacaaggggc agacaccctt
    41221 gtgcatcgac accgacccgg tgaacgcgac gttcgagggc tacaaggccc tgaacgtccg
    41281 ccggctgaac atcatggccg gcgacgaaat taactcgcgc aacttcgaca ccctggtcga
    41341 gctgattgcg ccgaccaagg atgacgtggt gatcgacaac ggtgccagct cgttcgtgcc
    41401 tctgtcgcat tacctcatca gcaaccaggt gccggctctg ctgcaagaaa tggggcatga
    41461 gctggtcatc cataccgtcg tcaccggcgg ccaggctctc ctggacacgg tgagcggctt
    41521 cgcccagctc gccagccagt tcccggccga agcgcttttc gtggtctggc tgaacccgta
    41581 ttgggggcct atcgagcatg agggcaagag ctttgagcag atgaaggcgt acacggccaa
    41641 caaggcccgc gtgtcgtcca tcatccagat tccggccctc aaggaagaaa cctacggccg
    41701 cgatttcagc gacatgctgc aagagcggct gacgttcgac caggcgctgg ccgatgaatc
    41761 gctcacgatc atgacgcggc aacgcctcaa gatcgtgcgg cgcggcctgt ttgaacagct
    41821 cgacgcggcg gccgtgctat gagcgaccag attgaagagc tgatccggga gattgcggcc
    41881 aagcacggca tcgccgtcgg ccgcgacgac ccggtgctga tcctgcatac catcaacgcc
    41941 cggctcatgg ccgacagtgc ggccaagcaa gaggaaatcc ttgccgcgtt caaggaagag
    42001 ctggaaggga tcgcccatcg ttggggcgag gacgccaagg ccaaagcgga gcggatgctg
    42061 aacgcggccc tggcggccag caaggacgca atggcgaagg taatgaagga cagcgccgcg
    42121 caggcggccg aagcgatccg cagggaaatc gacgacggcc ttggccgcca gctcgcggcc
    42181 aaggtcgcgg acgcgcggcg cgtggcgatg atgaacatga tcgccggcgg catggtgttg
    42241 ttcgcggccg ccctggtggt gtgggcctcg ttatgaatcg cagaggcgca gatgaaaaag
    42301 cccggcgttg ccgggctttg tttttgcgtt agctgggctt gtttgacagg cccaagctct
    42361 gactgcgccc gcgctcgcgc tcctgggcct gtttcttctc ctgctcctgc ttgcgcatca
    42421 gggcctggtg ccgtcgggct gcttcacgca tcgaatccca gtcgccggcc agctcgggat
    42481 gctccgcgca catcttgcgc gtcgccagtt cctcgatctt gggcgcgtga atgcccatgc
    42541 cttccttgat ttcgcgcacc atgtccagcc gcgtgtgcag ggtctgcaag cgggcttgct
    42601 gttgggcctg ctgctgctgc caggcggcct ttgtacgcgg cagggacagc aagccggggg
    42661 cattggactg tagctgctgc aaacgcgcct gctgacggtc tacgagctgt tctaggcggt
    42721 cctcgatgcg ctccacctgg tcatgctttg cctgcacgta gagcgcaagg gtctgctggt
    42781 aggtctgctc gatgggcgcg gattctaaga gggcctgctg ttccgtctcg gcctcctggg
    42841 ccgcctgtag caaatcctcg ccgctgttgc cgctggactg ctttactgcc ggggactgct
    42901 gttgccctgc tcgcgccgtc gtcgcagttc ggcttgcccc cactcgattg actgcttcat
    42961 ttcgagccgc agcgatgcga tctcggattg cgtcaacgga cggggcagcg cggaggtgtc
    43021 cggcttctcc ttgggtgagt cggtcgatgc catagccaaa ggtttccttc caaaatgcgt
    43081 ccattgctgg accgtgtttc tcattgatgc ccgcaagcat cttcggcttg accgccaggt
    43141 caagcgcgcc ttcatgggcg gtcatgacgg acgccgccat gaccttgccg ccgttgttct
    43201 cgatgtagcc gcgtaatgag gcaatggtgc cgcccatcgt cagcgtgtca tcgacaacga
    43261 tgtacttctg gccggggatc acctccccct cgaaagtcgg gttgaacgcc aggcgatgat
    43321 ctgaaccggc tccggttcgg gcgaccttct cccgctgcac aatgtccgtt tcgacctcaa
    43381 ggccaaggcg gtcggccaga acgaccgcca tcatggccgg aatcttgttg ttccccgccg
    43441 cctcgacggc gaggactgga acgatgcggg gcttgtcgtc gccgatcagc gtcttgagct
    43501 gggcaacagt gtcgtccgaa atcaggcgct cgaccaaatt aagcgccgct tccgcgtcgc
    43561 cctgcttcgc agcctggtat tcaggctcgt tggtcaaaga accaaggtcg ccgttgcgaa
    43621 ccaccttcgg gaagtctccc cacggtgcgc gctcggctct gctgtagctg ctcaagacgc
    43681 ctcccttttt agccgctaaa actctaacga gtgcgcccgc gactcaactt gacgctttcg
    43741 gcacttacct gtgccttgcc acttgcgtca taggtgatgc ttttcgcact cccgatttca
    43801 ggtactttat cgaaatctga ccgggcgtgc attacaaagt tcttccccac ctgttggtaa
    43861 atgctgccgc tatctgcgtg gacgatgctg ccgtcgtggc gctgcgactt atcggccttt
    43921 tgggccatat agatgttgta aatgccaggt ttcagggccc cggctttatc taccttctgg
    43981 ttcgtccatg cgccttggtt ctcggtctgg acaattcttt gcccattcat gaccaggagg
    44041 cggtgtttca ttgggtgact cctgacggtt gcctctggtg ttaaacgtgt cctggtcgct
    44101 tgccggctaa aaaaaagccg acctcggcag ttcgaggccg gctttcccta gagccgggcg
    44161 cgtcaaggtt gttccatcta ttttagtgaa ctgcgttcga tttatcagtt actttcctcc
    44221 cgctttgtgt ttcctcccac tcgtttccgc gtctagccga cccctcaaca tagcggcctc
    44281 ttcttgggct gcctttgcct cttgccgcgc ttcgtcacgc tcggcttgca ccgtcgtaaa
    44341 gcgctcggcc tgcctggccg cctcttgcgc cgccaacttc ctttgctcct ggtgggcctc
    44401 ggcgtcggcc tgcgccttcg ctttcaccgc tgccaactcc gtgcgcaaac tctccgcttc
    44461 gcgcctggtg gcgtcgcgct cgccgcgaag cgcctgcatt tcctggttgg ccgcgtccag
    44521 ggtcttgcgg ctctcttctt tgaatgcgcg ggcgtcctgg tgagcgtagt ccagctcggc
    44581 gcgcagctcc tgcgctcgac gctccacctc gtcggcccgc tgcgtcgcca gcgcggcccg
    44641 ctgctcggct cctgccaggg cggtgcgtgc ttcggccagg gcttgccgct ggcgtgcggc
    44701 cagctcggcc gcctcggcgg cctgctgctc tagcaatgta acgcgcgcct gggcttcttc
    44761 cagctcgcgg gcctgcgcct cgaaggcgtc ggccagctcc ccgcgcacgg cttccaactc
    44821 gttgcgctca cgatcccagc cggcttgcgc tgcctgcaac gattcattgg caagggcctg
    44881 ggcggcttgc cagagggcgg ccacggcctg gttgccggcc tgctgcaccg cgtccggcac
    44941 ctggactgcc agcggggcgg cctgcgccgt gtgctggcgt cgccattcgc gcatgccggc
    45001 gctggcgtcg ttcatgttga cgcgggcggc cttacgcact gcatccacgg tcgggaagtt
    45061 ctcccggtcg ccttgctcga acagctcgtc cgcagccgca aaaatgcggt cgcgcgtctc
    45121 tttgttcagt tccatgttgg ctccggtaat tggtaagaat aataatactc ttacctacct
    45181 tatcagcgca agagtttagc tgaacagttc tcgacttaac ggcaggtttt ttagcggctg
    45241 aagggcaggc aaaaaaagcc ccgcacggtc ggcgggggca aagggtcagc gggaagggga
    45301 ttagcgggcg tcgggcttct tcatgcgtcg gggccgcgct tcttgggatg gagcacgacg
    45361 aagcgcgcac gcgcatcgtc ctcggcccta tcggcccgcg tcgcggtcag gaacttgtcg
    45421 cgcgctaggt cctccctggt gggcaccagg ggcatgaact cggcctgctc gatgtaggtc
    45481 cactccatga ccgcatcgca gtcgaggccg cgttccttca ccgtctcttg caggtcgcgg
    45541 tacgcccgct cgttgagcgg ctggtaacgg gccaattggt cgtaaatggc tgtcggccat
    45601 gagcggcctt tcctgttgag ccagcagccg acgacgaagc cggcaatgca ggcccctggc
    45661 acaaccaggc cgacgccggg ggcaggggat ggcagcagct cgccaaccag gaaccccgcc
    45721 gcgatgatgc cgatgccggt caaccagccc ttgaaactat ccggccccga aacacccctg
    45781 cgcattgcct ggatgctgcg ccggatagct tgcaacatca ggagccgttt cttttgttcg
    45841 tcagtcatgg tccgccctca ccagttgttc gtatcggtgt cggacgaact gaaatcgcaa
    45901 gagctgccgg tatcggtcca gccgctgtcc gtgtcgctgc tgccgaagca cggcgagggg
    45961 tccgcgaacg ccgcagacgg cgtatccggc cgcagcgcat cgcccagcat ggccccggtc
    46021 agcgagccgc cggccaggta gcccagcatg gtgctgttgg tcgccccggc caccagggcc
    46081 gacgtgacga aatcgccgtc attccctctg gattgttcgc tgctcggcgg ggcagtgcgc
    46141 cgcgccggcg gcgtcgtgga tggctcgggt tggctggcct gcgacggccg gcgaaaggtg
    46201 cgcagcagct cgttatcgac cggctgcggc gtcggggccg ccgccttgcg ctgcggtcgg
    46261 tgttccttct tcggctcgcg cagcttgaac agcatgatcg cggaaaccag cagcaacgcc
    46321 gcgcctacgc ctcccgcgat gtagaacagc atcggattca ttcttcggtc ctccttgtag
    46381 cggaaccgtt gtctgtgcgg cgcgggtggc ccgcgccgct gtctttgggg atcagccctc
    46441 gatgagcgcg accagtttca cgtcggcaag gttcgcctcg aactcctggc cgtcgtcctc
    46501 gtacttcaac caggcatagc cttccgccgg cggccgacgg ttgaggataa ggcgggcagg
    46561 gcgctcgtcg tgctcgacct ggacgatggc ctttttcagc ttgtccgggt ccggctcctt
    46621 cgcgcccttt tccttggcgt ccttaccgtc ctggtcgccg tcctcgccgt cctggccgtc
    46681 gccggcctcc gcgtcacgct cggcatcagt ctggccgttg aaggcatcga cggtgttggg
    46741 atcgcggccc ttctcgtcca ggaactcgcg cagcagcttg accgtgccgc gcgtgatttc
    46801 ctgggtgtcg tcgtcaagcc acgcctcgac ttcctccggg cgcttcttga aggccgtcac
    46861 cagctcgttc accacggtca cgtcgcgcac gcggccggtg ttgaacgcat cggcgatctt
    46921 ctccggcagg tccagcagcg tgacgtgctg ggtgatgaac gccggcgact tgccgatttc
    46981 cttggcgata tcgcctttct tcttgccctt cgccagctcg cggccaatga agtcggcaat
    47041 ttcgcgcggg gtcagctcgt tgcgttgcag gttctcgata acctggtcgg cttcgttgta
    47101 gtcgttgtcg atgaacgccg ggatggactt cttgccggcc cacttcgagc cacggtagcg
    47161 gcgggcgccg tgattgatga tatagcggcc cggctgctcc tggttctcgc gcaccgaaat
    47221 gggtgacttc accccgcgct ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga
    47281 aaagccgggg ttgtcggccg tccgcggctg atgcggatct tcgtcgatca ggtccaggtc
    47341 cagctcgata gggccggaac cgccctgaga cgccgcagga gcgtccagga ggctcgacag
    47401 gtcgccgatg ctatccaacc ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc
    47461 ggccgcagcg gtgtttttct tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc
    47521 atcttcgtga acacgtaatc agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc
    47581 gttttcttga tcttccagac cggcacaccg gatgcgaggg catcggcgat gctgctgcgc
    47641 aggccaacgg tggccggaat catcatcttg gggtacgcgg ccagcagctc ggcttggtgg
    47701 cgcgcgtggc gcggattccg cgcatcgacc ttgctgggca ccatgccaag gaattgcagc
    47761 ttggcgttct tctggcgcac gttcgcaatg gtcgtgacca tcttcttgat gccctggatg
    47821 ctgtacgcct caagctcgat gggggacagc acatagtcgg ccgcgaagag ggcggccgcc
    47881 aggccgacgc caagggtcgg ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc
    47941 agggccttga tgttcgcccc gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg
    48001 ttcgccagta ccgggttgga ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct
    48061 gcgggtgcgg tttcggtcca gccgccggca gggacagcgc cgaacagctt gcttgcatgc
    48121 aggccggtag caaagtcctt gagcgtgtag gacgcattgc cctgggggtc caggtcgatc
    48181 acggcaaccc gcaagccgcg ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc
    48241 ttgccgacgc cgcctttctg gttggccgtg accaaagttt tcatcgtttg gtttcctgtt
    48301 ttttcttggc gtccgcttcc cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg
    48361 ccgttacccg cgcgtacccc tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat
    48421 gcaccgcttg cgacactgcg cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg
    48481 gcttcccatc gactaagacg ccccgcgcta tctcgatggt ctgctgcccc acttccagcc
    48541 cctggatcgc ctcctggaac tggctttcgg taagccgttt cttcatggat aacacccata
    48601 atttgctccg cgccttggtt gaacatagcg gtgacagccg ccagcacatg agagaagttt
    48661 agctaaacat ttctcgcacg tcaacacctt tagccgctaa aactcgtcct tggcgtaaca
    48721 aaacaaaagc ccggaaaccg ggctttcgtc tcttgccgct tatggctctg cacccggctc
    48781 catcaccaac aggtcgcgca cgcgcttcac tcggttgcgg atcgacactg ccagcccaac
    48841 aaagccggtt gccgccgccg ccaggatcgc gccgatgatg ccggccacac cggccatcgc
    48901 ccaccaggtc gccgccttcc ggttccattc ctgctggtac tgcttcgcaa tgctggacct
    48961 cggctcacca taggctgacc gctcgatggc gtatgccgct tctccccttg gcgtaaaacc
    49021 cagcgccgca ggcggcattg ccatgctgcc cgccgctttc ccgaccacga cgcgcgcacc
    49081 aggcttgcgg tccagacctt cggccacggc gagctgcgca aggacataat cagccgccga
    49141 cttggctcca cgcgcctcga tcagctcttg cactcgcgcg aaatccttgg cctccacggc
    49201 cgccatgaat cgcgcacgcg gcgaaggctc cgcagggccg gcgtcgtgat cgccgccgag
    49261 aatgcccttc accaagttcg acgacacgaa aatcatgctg acggctatca ccatcatgca
    49321 gacggatcgc acgaacccgc tgaattcacc cccgaacacg agcacggcac ccgcgaccac
    49381 tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc
    49441 gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg
    49501 gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg
    49561 gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgccgc
    49621 gatgaggaag cgggtgcccc gcttcttcat cttcgcgcct cgggcctcga ggccgcctac
    49681 ctgggcgaaa acatcggtgt ttgtggcatt catacggact cctgttgggc cagctcgcgc
    49741 acgggctggc gggtcagctt ggcttgaaga tcgccacgca ttgcggcgat ctgcttctcg
    49801 gcatccttgc gcttctgcac gccttcctgc tggatgcgaa taacgtcctc gacggtcttg
    49861 atgagcgtcg tctgaacctg cttgagcgtg tccacgtcga tcaccaggcg ttggttctcc
    49921 ttcgccgtct cgacggacgt gcgatgcagc agggccgcat tgcgcttcat caggtcgttg
    49981 gtggtgtcgt cgatggccgt ggccagttcg acggcgttct tctgctcgtt gaggctcaag
    50041 gccagcatga attgccgctt ccacgccggc acggtgattt cgcggatggt gtggaattta
    50101 tcgaccagca tctggttgtt ggcctggatc atgcggatgg tcggcaggct ctgcatggcc
    50161 gaatgttgca aggcgatcag gtcgccgatg cgcttgtcca ggttggcaac catcgcatcg
    50221 aggtcggcca gctcctgcac gcggcccggg tcgttcccga cattgccgcg cagaccctcg
    50281 gcctgctcgc gcagctcggc aaggcggacc ttgccggccg cgatgtggac gccaagaagg
    50341 cggtgttcct cgcgcacggc tgcgaacatt tcgtcgagcg aggcattgcg ctgcgcgatg
    50401 ccttgctggg tggtctgcac ttcgctgacc aggtgttcga tctgctcgcg ggtcgtgtcg
    50461 aagcgcgcca tgaagcccgt cgaacggacg cggaagcggt cgatcagcgg gccaatcagg
    50521 ggcaggcggg aacggttgtc ggacaaaggg ccgacgttca gggaacgggc cttggcgaca
    50581 acctgggtca gtttctcgcc tgcttcgtcc aggtcgctgt tgcgcacctg gtccagcagg
    50641 ctatcggcgt agcgggacgt gtgctcggcc acgtcgcggc cgaactcggc aacggtctgc
    50701 ggactgccga cctcgatccg ctgcgcgacc gcatggactt ccggcacgtc gctttcctgc
    50761 aagcccagct cgcgcagggt tgccggggtc atgtcgaagg cgacgatagg ggccttggcg
    50821 tcgtgcgtcg ttttcagtgc gttcataggg ttctcccgcc gtgttattgg ttgatgcctt
    50881 ccaggctctg cgaaaggctc cgcatgagcg cctggtgagc tttggccgcc tcggcgacca
    50941 ttgccggatt catgttcttg gtggtgatga gcgcgagggt gtgctgacgc cagacgggca
    51001 ccaggacgga tgccgtttca gagaagcggt ccagcatgtc cacggcctgc gcccgcgtga
    51061 gcttcatctg agtgacgctc atttcatggg acgccatgag ggttgccagg ttggcgagct
    51121 tgcgcgcgaa gcgttcgcgc ggcttgtcga actcgatcac gccggccttg gccgcgccgg
    51181 cctcggggtt ctcgtccagg aactcgcgcc cggcttgaat gtaggctctg agccggtcta
    51241 cctcggcctc atgcgtattg agcatgtcat ccaaggcgcg caacgtgtcc cgcacgcgct
    51301 gcgctacgcc ctcggcttcg tccagcaact ggtcgagcgt cttgcgggcg acctgatacc
    51361 tcacctggcg ttcaacctca cggccaagca tcttctcgaa ccaggtaggc ttttccgcga
    51421 tcttgcgggg gtccgcgtcg gccagcttcg ccacgatctg gctgattttg tcggccagcg
    51481 cggcaactgc gccgtgctcc atcagattcg acagctcgtt gagggaatcc gccccgtcga
    51541 tgccggcccc gtactcgcca atcgtcgccg gcgacgcgaa gagggcgggc aaaacctccc
    51601 ccttcaatcg cgccatgttc acgctttgtt cttccattcg atacaccctc gcggtgggtt
    51661 aattgctttt cgatggaaga agtttagcta aactttctat ccctcgtcaa cacctttagc
    51721 cgctaaaatt tggggacagg tcatttacag aaagccagct cactcctggc gttgcccctt
    51781 gagcgccgct aggcgcgcag catccttcgc gctgagaaag aacgtcatca gcggcccgac
    51841 cgtcttgctt gaaccgtcgg caaagcaaac atccatcgaa cagccttgcg tgtgggggtc
    51901 cacgccttcg accagtttcc aagggtccat gccccagccc tcggcctccg gattgaacca
    51961 gtacgccgat gcgtcgccgt ttaggtcgct gtcggcgtag tccttgacca gcacggccac
    52021 ccgcccggtc gtcgggcaca cgtagcccgg ctgcttaggt tcctgtcttg gcattgctca
    52081 aagctccttg aaggggccgc tctacagccc cttgggcttg tagagcgaca cgaaataggt
    52141 gagtgcggtc agtaccgcga aatgcaccag gaacgtccag ccggcatgaa cgccaagggt
    52201 gttccagtgg tacagcatcc gcaggaactg aaagaaaacg tcgatagaga tgatccactt
    52261 cgccaccggc cacaccagga cagtaacgac ccatacaaag cggaccaggg cctggacacc
    52321 cttggcaaaa gtgaaccggg gcggggcctt gctcggggcc tcaacgcgcg gggcaggggc
    52381 ctcggcctcc acttccacgc ctgggaactt gataatcttc gacattgctt gaccctccac
    52441 ggcgatgcgt gttcaattcg tccagcgctc gcgcgcctag accgtgatgt gacagcatcg
    52501 aggtcaagcg ccccggagaa atccggggcg tcatccctat gccccgtcca actcgggaac
    52561 cggcttttcc ctggtgctca acctggccgg ctcgacccac ttggtgactt gctgccactc
    52621 gttaccactg cgaacggcta cccgaatctg cacacgttta gccgcgatct tcgtcactat
    52681 gccagcaaca caaacggaat acccgtaccc gccgcgcggg gtgtgctgcc agttcaccct
    52741 atctcctact tgcatcatca tcccctggcg tcagtgaccg gcccggaatt tcgccaagtc
    52801 gatttcgttg aaggtccagc gctgtttcgc ccgttcctcc aacctcgacg actcccgcat
    52861 gacctcgatg cgcaggcgct cgacctggtg catcagctca tcagcgcgcc gctgcttctc
    52921 ggcaatagca ttccgctgcg cgaccagctc ctggtctacg ttcggcagct cgtcgatcca
    52981 cggcatgaac ttatcggtca tcggattggc ctccggtaat tgacctggga atctacccgg
    53041 cctcaaaaca agaatagggc ataatgccct aacttgtcaa gcaattttag ctaaacaatt
    53101 gaggggattc agcgaggcgt catgcttgaa aacacctttc ccctggcgtg caatcagctt
    53161 gtcccggcgg cagcgcactg ccgcagcgcg gccagcaagg tgccttgctc gatccgctgt
    53221 cgttcctccg gcgtaatgca atcgccgcag ataccgccca gctcttcgcg cagggcgtcc
    53281 gccccgatca ggctgcggct aagggtcgaa atggacttac cgcagcggcg gcaattggtg
    53341 gtgacaatct cgatcttgcg gttgggcatg gccctatctc cttgagagag gcccgaccgt
    53401 agccgggcct cgttccgtta ccagcctgcg cgagccttcg cgcgttcgac ttcctgggtg
    53461 gaccagtggc cctttgcttc accctccagg gtgcaacctt gcaggtacgc cttgtgccgc
    53521 agctcttcgg cctggcgggt aagctctgcc gcctgctcca tcagcgccgc gatctggtca
    53581 cgacgggcca gaaaatccgt ggcggccgcg ccgggcagct catcgagcca agacatgatc
    53641 ttgcttttca tcggggttga tcctccggtt gctgacctgg gcgaagtgcc cggccttgga
    53701 ttgctatatt agggcattat gccctagata gtcaaggaaa tttagctaaa caatttgcgg
    53761 cgggcgcacg aaaaaacccg gcttgcgggc cgggctgctg gcagcatatc gcaacgatca
    53821 gggttggcgg ttttagccgc taaagtcctc tcccttggcg taaagtcctg cgggcgtcag
    53881 ccctggcctt tccagatcgc cccaatcccc gctagatcgc aaaggatcgc ccaggcggca
    53941 taggggatcg gcgaatcctc gccaacccaa cgccgcaccg tgcggtcgcc cttggcaccc
    54001 aagcccaaga tgcgcgcagc ctgtccgccg gtgaggccgg ccaagtgcaa gacttcccgg
    54061 atttctgcgc cggtcggctg cacccagcgt tccgccgggc gcaggcactc aagccggata
    54121 ttcacgtcgc tcatgctgct tttctcctaa tcgttatcaa atggcggccc gcaattggtc
    54181 gtagccgtag cacgactcga tgcaacgcgg gtcatattca caaacctttc taccgttgcg
    54241 attgatgcga tagcgcggcg gcttgtcgtc gtgcgcccac acctgtcgcg tttcgggact
    54301 gatcttcgta atgatgacgt gcttcccgat ttccttggca aaaaccgggt gatgcacgct
    54361 cacaatctcg caccgcaggc caggaaagaa cgtcgccggc cacgccggcg cgtcctcgtc
    54421 cttggccggc tccggctccg gcttggccgg tgtaaccggc tccctgcgct gcccggccgg
    54481 ctcctgagct ttcgcacgcg cggccgcgag cttcgccttg gcgctggccg cccatttgcg
    54541 ggcgatgaat gcctggtgcg ccggcagcac ggcatcaacc caggcgaagc ccggcggcag
    54601 ttcgtccggc ttccgccagt cctcgaccac ggcccgcacg cggcgcggcg gtggcgtgat
    54661 acccgcgagc cacgccggca gtggctcgtc ggcaaggcgg tcctggtcgc gggcgtccag
    54721 ataccggcga tgcgagcgca acagggcgcg caaaaccccg tccgctacct ggtccaccgt
    54781 catgccgccg cgcgcatggt cgtaatggga ccgatagccc gtttcggaaa taaaaggggt
    54841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    54901 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    54961 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    55021 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    55081 gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca cgctcaccgg
    55141 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    55201 caactttatc cgcctccatc cagtctatta atccacctgg cggcgttgtg acaatttacc
    55261 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
    55321 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
    55381 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
    55441 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctctccgga
    55501 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
    55561 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
    55621 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
    55681 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
    55741 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
    55801 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
    55861 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
    55921 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
    55981 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
    56041 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtccatggg
    56101 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
    56161 cgtttgtgat ggcttccatg tcggcaggaa ttcgaattca tacccaccgg ctccaactgc
    56221 gcggcctgcg gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc
    56281 tgcggcctgc gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc
    56341 gcagcgaccg cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt
    56401 gggggcagtc gaagggcgaa gcccgcccgc ctgccccccg agcctcacgg
//
