LOCUS       pAV0279                 3913 bp    DNA     linear       28-APR-2020
FEATURES             Location/Qualifiers
     PCR_primer      1..1701
                     /dnas_title="CCAAATAAGCTTGACACTAGAGGCATCAAAT"
                     /primer="CCAAATAAGCTTGACACTAGAGGCATCAAAT"
                     /note="SF526"
                     /set="Set 1"
     PCR_primer      1..1723
                     /dnas_title="GGGTCTAAGCTTGGGGTAAAACGAAAGGCTCAGTCGAAA"
                     /primer="GGGTCTAAGCTTGGGGTAAAACGAAAGGCTCAGTCGAAA"
                     /note="SF1151"
                     /set="Set 1"
     PCR_primer      1..1723
                     /dnas_title="GGGTCTACTAGTGGGGTAAAACGAAAGGCTCAGTCGAAA"
                     /primer="GGGTCTACTAGTGGGGTAAAACGAAAGGCTCAGTCGAAA"
                     /note="SF1152"
                     /set="Set 1"
     misc_feature    115..186
                     /note="His14-TAG"
     misc_feature    193..480
                     /note="SUMO_Eu1"
     misc_feature    25..30
                     /note="-35 box"
     misc_feature    7..23
                     /note="-43 AT rich region"
     misc_feature    48..53
                     /note="-10 box"
     misc_feature    60..60
                     /note="transcription start"
     misc_feature    62..80
                     /note="lacO"
     misc_feature    30..48
                     /note="lacO"
     misc_feature    96..106
                     /note="RBS"
     misc_feature    499..1599
                     /note="MBP"
     misc_feature    1692..1797
                     /note="t1 terminator"
     misc_feature    1801..2598
                     /note="p15A origin"
     PCR_primer      complement(1749..1771)
                     /dnas_title="CAGGAGAGCGTTCACCGACAAAC"
                     /primer="CAGGAGAGCGTTCACCGACAAAC"
                     /note="P45"
                     /set="Set 1"
     PCR_primer      complement(1769..1802)
                     /dnas_title="GTGTGTGTCGACGCGGCGGATTTGTCCTACTCAG"
                     /primer="GTGTGTGTCGACGCGGCGGATTTGTCCTACTCAG"
                     /note="SF1220"
                     /set="Set 1"
     PCR_primer      complement(1772..1807)
                     /dnas_title="TCTGCACGTAAGCATGCGCGGCGGATTTGTCCTACT"
                     /primer="TCTGCACGTAAGCATGCGCGGCGGATTTGTCCTACT"
                     /note="SF1235"
                     /set="Set 1"
     misc_feature    1612..1677
                     /note="3x Flag-Tag"
     misc_feature    1582..1599
                     /note=""
     misc_feature    complement(2601..2714)
                     /note="t0 terminator"
     misc_feature    complement(2749..3681)
                     /note="Spectinomycin resistance"
     PCR_primer      2670..2688
                     /dnas_title="GTTCTGAGGTCATTACTGG"
                     /primer="GTTCTGAGGTCATTACTGG"
                     /note="pQE-R"
                     /set="Set 1"
     PCR_primer      complement(2670..2738)
                     /dnas_title="CTAGTTGCTGTTAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGA
                     TAGATCCAGTAATGACCTCAGAAC"
                     /primer="CTAGTTGCTGTTAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGA
                     TCCAGTAATGACCTCAGAAC"
                     /note="SF616_mut613ff_End"
                     /set="Set 1"
     PCR_primer      complement(2699..2724)
                     /dnas_title="GCTTAATTAGCTGAGCTTGGACTCCT"
                     /primer="GCTTAATTAGCTGAGCTTGGACTCCT"
                     /note="Kan"
                     /set="Set 1"
     PCR_primer      3887..3906
                     /dnas_title="CGTATCACGAGGCCCTTTCG"
                     /primer="CGTATCACGAGGCCCTTTCG"
                     /note="pQE-F"
                     /set="Set 1"
     misc_feature    412..414
                     /note=""
     misc_feature    367..369
                     /note=""
     misc_feature    388..390
                     /note=""
     source          1..3913
                     /dnas_title="pAV0279 H14-SUMO_Eu1-MBP-3xFlagTag"
ORIGIN      
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAaG AGGaGAAATT AAccatgagc
      121 aagcatcacc atcattcagg ccatcaccat accggacacc accatcattc aggcagtcat
      181 caccatTCCG GATCTGCTGC GGGTGGCGAA GAAGATAAGA AACCGGCAGG TGGCGAAGGT
      241 GGCGGTGCCC ATATCAACCT GAAAGTGAAA GGTCAAGACG GCAACGAAGT CTTTTTCCGC
      301 ATCAAACGTT CTACCCAGCT GAAAAAGCTG ATGAACGCAT ACTGTGACCG TCAGTCTGTA
      361 GACATGaagG CAATTGCTTT CCTCTTTaag GGTCGTCGCC TACGTGCGGA AaggACCCCG
      421 GATGAACTGG AAATGGAAGA TGGCGACGAA ATCGACGCAA TGCTGCACCA GACTGGAGGC
      481 GCCGgtaccg gtactagtAA AACTGAAGAA GGTAAACTGG TAATCTGGAT TAACGGCGAT
      541 AAAGGCTATA ACGGTCTCGC TGAAGTCGGT AAGAAATTCG AGAAAGATAC CGGAATTAAA
      601 GTCACCGTTG AGCATCCGGA TAAACTGGAA GAGAAATTCC CACAGGTTGC GGCAACTGGC
      661 GATGGCCCTG ACATTATCTT CTGGGCACAC GACCGCTTTG GTGGCTACGC TCAATCTGGC
      721 CTGTTGGCTG AAATCACCCC GGACAAAGCG TTCCAGGACA AGCTGTATCC GTTTACCTGG
      781 GATGCCGTAC GTTACAACGG CAAGCTGATT GCTTACCCGA TCGCTGTTGA AGCGTTATCG
      841 CTGATTTATA ACAAAGATCT GCTGCCGAAC CCGCCAAAAA CCTGGGAAGA GATCCCGGCG
      901 CTGGATAAAG AACTGAAAGC GAAAGGTAAG AGCGCGCTGA TGTTCAACCT GCAAGAACCG
      961 TACTTCACCT GGCCGCTGAT TGCTGCTGAC GGGGGTTATG CGTTCAAGTA TGAAAACGGC
     1021 AAGTACGACA TTAAAGACGT GGGCGTGGAT AACGCTGGCG CGAAAGCGGG TCTGACCTTC
     1081 CTGGTTGACC TGATTAAAAA CAAACACATG AATGCAGACA CCGATTACTC CATCGCAGAA
     1141 GCTGCCTTTA ATAAAGGCGA AACAGCGATG ACCATCAACG GCCCGTGGGC ATGGTCCAAC
     1201 ATCGACACCA GCAAAGTGAA TTATGGTGTA ACGGTACTGC CGACCTTCAA GGGTCAACCA
     1261 TCCAAACCGT TCGTTGGCGT GCTGAGCGCA GGTATTAACG CCGCCAGTCC GAACAAAGAG
     1321 CTGGCAAAAG AGTTCCTCGA AAACTATCTG CTGACTGATG AAGGTCTGGA AGCGGTTAAT
     1381 AAAGACAAAC CGCTGGGTGC CGTAGCGCTG AAGTCTTACG AGGAAGAGTT GGCGAAAGAT
     1441 CCACGTATTG CCGCCACCAT GGAAAACGCC CAGAAAGGTG AAATCATGCC GAACATCCCG
     1501 CAGATGTCCG CTTTCTGGTA TGCCGTGCGT ACTGCGGTGA TCAACGCCGC CAGCGGTCGT
     1561 CAGACTGTCG ATGAAGCCCT GAAAGACGCG CAGACTAATg gatctGGTTC TGATTATAAA
     1621 GATCATGATG GCGATTATAA AGATCATGAT ATTGATTATA AAGATGATGA TGATAAAtAa
     1681 gcTTCTCTAG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     1741 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACCTA
     1801 GGGATATATT CCGCTTCCTC GCTCACTGAC TCGCTACGCT CGGTCGTTCG ACTGCGGCGA
     1861 GCGGAAATGG CTTACGAACG GGGCGGAGAT TTCCTGGAAG ATGCCAGGAA GATACTTAAC
     1921 AGGGAAGTGA GAGGGCCGCG GCAAAGCCGT TTTTCCATAG GCTCCGCCCC CCTGACAAGC
     1981 ATCACGAAAT CTGACGCTCA AATCAGTGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     2041 AGGCGTTTCC CCCTGGCGGC TCCCTCGTGC GCTCTCCTGT TCCTGCCTTT CGGTTTACCG
     2101 GTGTCATTCC GCTGTTATGG CCGCGTTTGT CTCATTCCAC GCCTGACACT CAGTTCCGGG
     2161 TAGGCAGTTC GCTCCAAGCT GGACTGTATG CACGAACCCC CCGTTCAGTC CGACCGCTGC
     2221 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGAAA GACATGCAAA AGCACCACTG
     2281 GCAGCAGCCA CTGGTAATTG ATTTAGAGGA GTTAGTCTTG AAGTCATGCG CCGGTTAAGG
     2341 CTAAACTGAA AGGACAAGTT TTGGTGACTG CGCTCCTCCA AGCCAGTTAC CTCGGTTCAA
     2401 AGAGTTGGTA GCTCAGAGAA CCTTCGAAAA ACCGCCCTGC AAGGCGGTTT TTTCGTTTTC
     2461 AGAGCAAGAG ATTACGCGCA GACCAAAACG ATCTCAAGAA GATCATCTTA TTAATCAGAT
     2521 AAAATATTTC TAGATTTCAG TGCAATTTAT CTCTTCAAAT GTAGCACCTG AAGTCAGCCC
     2581 CATACGATAT AAGTTGTTgC TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA
     2641 ACCGAGCGTT CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG
     2701 GAGTCCAAGC GAGCTCGATA TCCGTCGGCT TGAACGAATT GTTAGACATT ATTTGCCGAC
     2761 TACCTTGGTG ATCTCGCCTT TCACGTAGTG GACAAATTCT TCCAACTGAT CTGCGCGCGA
     2821 GGCCAAGCGA TCTTCTTCTT GTCCAAGATA AGCCTGTCTA GCTTCAAGTA TGACGGGCTG
     2881 ATACTGGGCC GGCAGGCGCT CCATTGCCCA GTCGGCAGCG ACATCCTTCG GCGCGATTTT
     2941 GCCGGTTACT GCGCTGTACC AAATGCGGGA CAACGTAAGC ACTACATTTC GCTCATCGCC
     3001 AGCCCAGTCG GGCGGCGAGT TCCATAGCGT TAAGGTTTCA TTTAGCGCCT CAAATAGATC
     3061 CTGTTCAGGA ACCGGATCAA AGAGTTCCTC CGCCGCTGGA CCTACCAAGG CAACGCTATG
     3121 TTCTCTTGCT TTTGTCAGCA AGATAGCCAG ATCAATGTCG ATCGTGGCTG GCTCGAAGAT
     3181 ACCTGCAAGA ATGTCATTGC GCTGCCATTC TCCAAATTGC AGTTCGCGCT TAGCTGGATA
     3241 ACGCCACGGA ATGATGTCGT CGTGCACAAC AATGGTGACT TCTACAGCGC GGAGAATCTC
     3301 GCTCTCTCCA GGGGAAGCCG AAGTTTCCAA AAGGTCGTTG ATCAAAGCTC GCCGCGTTGT
     3361 TTCATCAAGC CTTACGGTCA CCGTAACCAG CAAATCAATA TCACTGTGTG GCTTCAGGCC
     3421 GCCATCCACT GCGGAGCCGT ACAAATGTAC GGCCAGCAAC GTCGGTTCGA GATGGCGCTC
     3481 GATGACGCCA ACTACCTCTG ATAGTTGAGT CGATACTTCG GCGATCACCG CTTCCCTCAT
     3541 GATGTTTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTCCATAA
     3601 CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG
     3661 TACCCCAAAA AAACATGTCA TAACAAGAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC
     3721 CGCTGCGTTC GGTCAAGGTT GTGGACCAGT TGCGTGACGG CAGTTACGCT ACTTGCATTA
     3781 CAGCTTACGA ACCGAACGAG GCTTATGTCC ACTGGGTTCG TGCCTTCATC CGGATATCGA
     3841 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC
     3901 CTTTCGTCTT CAC
//

