LOCUS       Exported               13243 bp ds-DNA     circular UNA 01-MAY-2020
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...4v-9j1odqd
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 1, 2020 from SnapGene Viewer 5.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    791..933
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(1035..1226)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        2789..2807
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2856..2864
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             2857..2862
     CDS             2875..3819
                     /standard_name="RpoA"
                     /label=RpoA
     CDS             3938..7519
                     /standard_name="RpoB"
                     /label=RpoB
     promoter        7543..7561
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             7594..7599
     CDS             7606..7809
                     /standard_name="YloH"
                     /label=RpoZ
     CDS             7853..11485
                     /standard_name="RpoC-9His"
                     /label=RpoC-9His
     CDS             11456..11485
                     /standard_name="9xHis Tag"
                     /label=9xHis Tag
     misc_feature    11485^11486
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Deletion"
     terminator      11566..11613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(11979..12007)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        12124..12228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12229..13089
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      841 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
      901 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
      961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     1081 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     1141 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     1201 agggcggttt tttcctgttt ggtcacttga tgcctccgtg taagggggaa tttctgttca
     1261 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     1321 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     1381 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     1441 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     1501 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     1561 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     1621 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     1681 tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg
     1741 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
     1801 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
     1861 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
     1921 aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc
     1981 ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag
     2041 cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     2101 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     2161 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat
     2221 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     2281 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     2341 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     2401 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     2461 tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag
     2521 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     2581 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     2641 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     2701 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     2761 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg
     2821 tttccctcta gtaataattt tgtttaatcg gatcataagg aggttaatca tatgatgatc
     2881 gagattgaaa aaccaaaaat cgaaacggtt gaaatcagcg acgatgccaa atttggtaag
     2941 tttgtcgtag agccacttga gcgtggatat ggtacaactc tgggtaactc cttacgtcgt
     3001 atcctcttat cctcactccc tggtgccgct gtaacatcaa tccagataga tggtgtactg
     3061 cacgaattct cgacaattga aggcgttgtg gaagatgtta caacgattat cttacacatt
     3121 aaaaagcttg cattgaaaat ctactctgat gaagagaaga cgctagaaat tgatgtacag
     3181 ggtgaaggaa ctgtaacggc agctgatatt acacacgata gtgatgtaga gatcttaaat
     3241 cctgatcttc atatcgcgac tcttggtgag aatgcgagtt tccgagttcg ccttactgct
     3301 caaagaggac gtgggtatac gcctgctgac gcaaacaaga gagacgatca gccaatcggc
     3361 gtgattccga tcgattctat ctatacgcca gtttctcgtg tatcttatca ggtagagaac
     3421 actcgtgtag gccaagttgc aaactatgat aaacttacac ttgatgtttg gactgatgga
     3481 agcactggac cgaaagaagc aattgcgctt ggttcaaaga ttttaactga acaccttaat
     3541 atattcgttg gtttaactga cgaagctcaa catgctgaaa tcatggttga aaaagaagaa
     3601 gatcaaaaag agaaagttct tgaaatgaca attgaagaat tggatctttc tgttcgttct
     3661 tacaactgct taaagcgtgc gggtattaac acggttcaag agcttgcgaa caagacggaa
     3721 gaagatatga tgaaagttcg aaatctagga cgcaaatcac ttgaagaagt gaaagcgaaa
     3781 ctagaagaac ttggactcgg acttcgcaaa gacgattgac tagatgtggt tttcttaatt
     3841 agaaaccctt ttttcttttg tcttgtaaaa gtattttctt tctttggaag aaatgcttat
     3901 tacacatata atacgcatga tttgaggggt gaatcagttg acaggtcaac tagttcagta
     3961 tggacgacac cgccagcgca gaagctatgc tcgcattagc gaagtgttag aattaccaaa
     4021 tctcattgaa attcaaacct cttcttatca gtggtttctt gatgagggtc ttagagagat
     4081 gtttcaagac atatcaccaa ttgaggattt cactggtaac ctctctcttg agttcattga
     4141 ttatagttta ggtgagccta aatatcctgt agaggaatca aaagaacgtg atgtgactta
     4201 ctcagctccg ctaagagtga aggttcgttt aattaacaaa gaaactggag aggtaaaaga
     4261 ccaagatgtc ttcatgggtg atttccctat tatgacagat acaggtactt ttatcattaa
     4321 cggtgcggaa cgtgttatcg tttcccagct tgttcggtct ccaagtgtat atttcagtgg
     4381 taaagtagac aaaaacggta aaaaaggttt taccgcaact gtcattccaa accgtggcgc
     4441 atggttagaa tacgaaactg atgcgaaaga tgttgtttat gtccgcattg atcgcacacg
     4501 taagttgccg gttacggttc ttttgcgtgc tctcggcttc ggctccgatc aagagattct
     4561 tgatctcata ggagaaaacg aatacctgcg aaatacgctt gataaagata acacagaaaa
     4621 cagcgacaaa gcgttgctgg aaatttacga gcgtctccgt cctggagagc cgcctacagt
     4681 agaaaatgcg aaaagcttgc ttgattctcg tttctttgat ccgaaacgat acgatcttgc
     4741 caatgtagga cgctataaaa ttaataaaaa acttcatatt aagaatcgcc tcttcaatca
     4801 gagacttgct gaaacgcttg ttgatcctga aacaggagaa atccttgctg aaaaaggcca
     4861 gattcttgat agaagaacgc ttgataaagt actgccatac ttagaaaacg gaatcggttt
     4921 tagaaagctg tatccgaatg gcggcgttgt tgaagatgaa gtgactcttc aatcaattaa
     4981 aatctttgct ccgactgatc aagaaggaga acaggttatc aatgtaatcg gcaatgctta
     5041 catcgaagaa gagattaaaa acatcacgcc tgctgatatt atttcttcaa tcagctactt
     5101 cttcaacctg ctgcacggag taggcgacac agatgatatc gatcatcttg gaaaccgccg
     5161 tttacgttct gtaggcgagc ttctccagaa ccaattccgt atcggtttaa gccgtatgga
     5221 gcgtgtggtt cgtgagagaa tgtcaattca agatacgaat acaattacgc ctcagcagct
     5281 gatcaatatt cgtcctgtta ttgcgtccat taaagagttc tttggaagct cacagctttc
     5341 tcaattcatg gatcagacga acccgcttgc tgaattaacg cacaagcgtc gtctgtcagc
     5401 attaggaccg ggcggattga cacgtgagcg tgccggaatg gaagtgcgtg acgttcacta
     5461 ctcccactat ggccgtatgt gtccgattga aacgcctgag ggcccgaaca tcggtttgat
     5521 caactcacta tcatcttatg caaaagtaaa ccgttttggc tttattgaaa cgccatatcg
     5581 ccgcgttgac cctgaaacag ggaaggtaac gggcagaatc gattacttaa ctgctgatga
     5641 agaggataac tatgttgtcg ctcaagcgaa tgctcgtctt gatgacgaag gcgcctttat
     5701 tgatgacagc atcgtagctc gtttccgcgg ggagaacacc gttgtttcca gaaatcgtgt
     5761 agactacatg gatgtatcgc ctaagcaggt tgtatctgct gcgacagcat gtatcccgtt
     5821 cttagaaaac gatgactcca accgtgccct catgggagcg aacatgcagc gtcaggctgt
     5881 gcctttgatg cagccggaag cgccatttgt tggaactggt atggaatacg tatcaggaaa
     5941 agactctggt gccgctgtta tttgtaaaca ccctggtatc gttgaacgcg tagaagccaa
     6001 aaacgtttgg gttcgccgtt atgaagaagt agacggtcaa aaagtaaaag gaaacctgga
     6061 taaatacagc ctgctgaaat ttgtccgctc taaccaaggt acgtgctaca accagcgtcc
     6121 gatcgtaagt gtcggcgatg aagtggtaaa aggagaaatc cttgctgacg gtccttctat
     6181 ggagcttggt gaacttgcac ttggccgtaa cgtaatggtc ggcttcatga cgtgggatgg
     6241 ctacaactat gaggatgcca tcatcatgag tgaacgccta gtgaaggatg atgtttatac
     6301 atctatccac attgaagaat acgaatcaga agcacgtgat acgaaacttg gacctgaaga
     6361 aatcactcgc gatattccaa acgtcggtga agatgcgctt cgcaatcttg atgaccgcgg
     6421 aatcatccgt attggggcag aagtaaaaga cggagatctt cttgttggta aagtaacgcc
     6481 taaaggcgta actgaactga ctgccgaaga acgccttctt cacgccatct ttggcgagaa
     6541 agcccgcgag gttcgtgata cttctcttcg tgtgcctcat ggcggcggcg gaattatcca
     6601 tgacgttaaa gtcttcaacc gtgaagacgg agacgaactt cctccaggtg ttaaccagtt
     6661 agtacgcgta tatatcgttc agaaacgtaa gatttctgaa ggggataaaa tggccggtcg
     6721 tcacggtaac aaaggtgtta tctctaagat tcttcctgaa gaggatatgc cttaccttcc
     6781 tgacggcaca ccaattgata tcatgcttaa cccgctgggc gtaccatcac gtatgaacat
     6841 cgggcaggta ttggaacttc acatgggtat ggccgctcgt taccttggca ttcacattgc
     6901 atctcctgta tttgacggag cgcgagaaga ggatgtctgg gaaacacttg aagaagccgg
     6961 catgtctcgt gacgccaaaa cagtgcttta cgacggacgt actggagagc cgtttgataa
     7021 ccgtgtatct gtcggtatca tgtacatgat caaactggct cacatggttg acgataaact
     7081 tcatgcacgc tctacaggcc cttactcact tgttacgcag cagcctcttg gcggtaaagc
     7141 gcaatttggc ggacagcgtt ttggtgagat ggaggtttgg gcacttgaag cttacggtgc
     7201 ggcttacact cttcaagaaa ttctgactgt taagtctgat gacgtggttg gacgtgtgaa
     7261 aacatacgaa gccatcgtta aaggcgacaa tgttcctgaa ccaggtgttc cggaatcatt
     7321 caaagtatta atcaaagaac ttcaaagctt aggtatggat gtcaaaatcc tttctggtga
     7381 tgaagaagaa atagaaatga gagatttaga agacgaagaa gatgcgaaac aagctgacgg
     7441 cctggcatta tcaggtgatg aagagccgga agaaacagca tctgcagacg ttgaacgcga
     7501 tgtagtaaca aaagaataat ctctacttat agcgagcact cgtaatacga ctcactatag
     7561 gtattgccca tggtttagca tacctctaga ccaaaggagc ctatgatgtt agatccgtca
     7621 attgattctt taatgaataa attagattca aaatatacgc tggtgactgt ttctgcgaga
     7681 cgtgcccgtg aaatgcaaat caaaaaagac caaatgattg aacatacgat ttcacacaaa
     7741 tatgtaggca aagctttaga agaaattgat gcaggcctgc tttcgtttga aaaggaagac
     7801 cgcgaatagg ctgacatcgg ttaatccgaa gataaaaagg gaggtaggcc ccttgctaga
     7861 tgtgaacaat tttgagtata tgaacatcgg tcttgcttca ccagataaaa tccgttcatg
     7921 gtcttttggt gaagtgaaaa agcctgaaac gataaactat cgtacgttaa aacctgaaaa
     7981 ggacggtcta ttctgcgaac gcattttcgg accgactaag gactgggaat gtcattgcgg
     8041 gaagtacaag cgagttcgtt ataaaggcgt agtttgtgac cgctgcggag tcgaagtaac
     8101 acgggctaaa gtccgtcgtg agagaatggg gcacattgaa ctggctgccc cagtttccca
     8161 catttggtat ttcaaaggta ttccaagccg tatgggtctt gtgctggata tgtcacctcg
     8221 tgctttagaa gaagtcattt actttgcttc ttacgttgta actgatccgg cgaatacacc
     8281 gcttgaaaag aaacagcttc tgtctgagaa agaataccgt gcttatcttg ataaatacgg
     8341 taataaattc caagcatcta tgggtgctga agcaattcat aaacttcttc aagatatcga
     8401 tcttgtaaaa gaagttgata tgttaaaaga agagctgaaa acttcacaag gacaacgccg
     8461 tactcgtgcg atcaaacgtc ttgaagtttt agaagccttc cgtaactcag gaaacaagcc
     8521 ttcttggatg atccttgatg tgcttcctgt tattcctccg gagcttagac cgatggttca
     8581 gctagatggc ggacgttttg cgacttctga tttgaatgac ctttatcgtc gtgtcatcaa
     8641 ccgtaacaat cgtttgaaac gccttttgga ccttggtgcg cctagcatca tcgttcaaaa
     8701 cgaaaagcgt atgcttcaag aggctgtcga tgccctaatt gacaacggcc gccgcggacg
     8761 ccctgtaaca ggtcctggaa acagaccgtt aaaatctctt tctcacatgc tgaaagggaa
     8821 gcaaggccgt ttccgtcaaa accttcttgg taaacgtgtc gattactccg gacgttctgt
     8881 aatcgttgtt ggtcctcatt tgaaaatgta ccaatgcgga ttaccgaagg aaatggcact
     8941 tgaacttttc aaacctttcg ttatgaaaga acttgttgaa aaaggtcttg ctcacaacat
     9001 taagagtgcg aaacgcaaaa ttgagcgcgt acagcctgaa gtttgggatg tactagaatc
     9061 agttattaag gagcatccgg tactgctgaa ccgtgcccct acacttcaca gattaggtat
     9121 ccaggcgttt gaaccaacgc ttgttgaagg acgcgcaatc cgtcttcacc cgctcgtatg
     9181 tacagcttac aacgctgact ttgacggtga ccaaatggcg gttcacgtac cattatctgc
     9241 tgaagcacaa gctgaagcac gcatcttgat gcttgctgct caaaacatct tgaaccctaa
     9301 agatggtaaa ccggttgtaa caccgtctca ggatatggta ctaggtaact attacctgac
     9361 acttgagcgt gccggtgctg tcggtgaagg tatggtcttc aagaatacag acgaagcgct
     9421 tcttgcttat caaaacggat atgttcacct tcatacgaga gtagctgttg cagctaactc
     9481 acttaagaat gtgacattta ccgaagaaca gcgctcaaaa ttgttaatta caactgtcgg
     9541 taagcttgtc ttcaatgaaa ttcttccgga atcattccct tacatgaatg aaccaacgaa
     9601 gagcaacatt gaagagaaaa cacctgaccg tttcttctta gaaaaaggtg ctgatgttaa
     9661 agctgttatc gcacagcagc caatcaatgc gccgtttaaa aaaggcattc tgggtaaaat
     9721 catcgcggaa atctttaaac gattccatat tacggaaacg tctaaaatgc ttgaccgcat
     9781 gaaaaaccta ggtttcaaat attcaactaa agctggtatt acagttgggg tttctgacat
     9841 cgtcgtactc gatgataaac aagaaattct tgaggaagcg caaagcaaag ttgacaacgt
     9901 tatgaagcaa ttccgccgcg gtcttatcac tgaagaagaa cgctatgaga gagtcatctc
     9961 tatctggagt gctgcaaaag acgttatcca aggcaaactg atgaaatcac ttgatgaact
    10021 caacccgatc tacatgatga gtgactctgg tgcccgtggt aacgcatcta actttacgca
    10081 gcttgccgga atgcgcggcc tgatggccaa cccggctgga cgtatcattg agttgccgat
    10141 caaatcaagt ttccgtgaag gtctgacagt attggagtac tttatctcca cacacggtgc
    10201 gcgtaaaggt cttgccgata ccgctcttaa aactgctgac tcaggttacc ttacacgccg
    10261 tctcgttgac gttgctcagg atgttatcat ccgtgaaact gattgcggaa ctgaccgagg
    10321 cattcttgct aagcctctta aagaaggaac tgaaacaatt gagcgcttag aagaacgctt
    10381 aatcggacgt tttgcaagaa aacaagtgaa gcaccctgaa acaggtgaag tgcttgtgaa
    10441 tgaaaacgaa ctgatcgatg aagataaagc actggagatt gtcgaagccg gcattgaaga
    10501 agtgtggatc cgttctgcct tcacatgtaa tacgcctcat ggtgtatgta aacgatgcta
    10561 tggccgaaat cttgcaactg gctccgatgt tgaagtcggt gaagctgtcg gaatcattgc
    10621 tgcccaatca atcggtgagc ctggtacaca gttaacaatg cgtacattcc atacaggcgg
    10681 ggttgccgga gacgatatca cacagggtct tccgcgtatc caagagcttt tcgaagcgcg
    10741 taatccgaaa ggtcaggcaa caattacaga aatcgacggt acagtcgttg agatcaatga
    10801 agttcgtgat aagcaacagg aaattgtggt tcaaggcgca gtggaaacac gttcttatac
    10861 ggcaccttac aactcccgcc tgaaagtagc ggaaggagat aaaattactc gaggccaagt
    10921 actgacagaa ggttcaatcg atccgaaaga acttcttaaa gtgactgacc tgacgactgt
    10981 tcaagagtat cttctccatg aggttcaaaa ggtttaccgt atgcagggtg ttgaaatcgg
    11041 tgataaacac gtagaagtaa tggttcgcca gatgcttcgc aaagtacgcg tgattgatgc
    11101 cggtgacact gatgtgcttc caggtacatt gcttgatatt caccaattta ctgaagcgaa
    11161 caaaaaggta ttgcttgaag gcaaccgacc agctacaggc cgtcctgtct tactcggtat
    11221 tacaaaagca tctcttgaaa ctgattcatt cttatctgct gcttccttcc aggaaacaac
    11281 acgtgtcctt acagatgcag cgatcaaagg taagcgtgac gagcttctcg gcttgaaaga
    11341 gaatgttatc atcggtaagc ttgttccagc aggtacagga atgatgaaat accgtaaagt
    11401 aaaaccagta tcaaatgttc agccgactga cgatatggtc ccggttgaag gtacccacca
    11461 tcaccatcac catcaccatc actaacatgg catgcatcga tagatccggc tgctaacaaa
    11521 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
    11581 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
    11641 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
    11701 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
    11761 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
    11821 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
    11881 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
    11941 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag
    12001 ctgtcaaaca tgagaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
    12061 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    12121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    12181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    12241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    12301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    12361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    12421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
    12481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    12541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    12601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    12661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    12721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
    12781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    12841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    12901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    12961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    13021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    13081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    13141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    13201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaa
//
