LOCUS       pEnt223_ACE2_Ope        5211 bp ds-DNA     circular     07-APR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid .
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   gingras
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2020 from SnapGene 4.2.4 http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      268..295
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
                     /label=rrnB T2 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /label=rrnB T1 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     537..553
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 fwd
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label=attL1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             674..3088
                     /created_by="User"
                     /label=ACE2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    complement(3090..3189)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label=attL2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3230..3246)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3677..4468
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /label=SmR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4561..5149
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label=ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatgtcaa gctcttcctg gctccttctc agccttgttg ctgtaactgc
      721 tgctcagtcc accattgagg aacaggccaa gacatttttg gacaagttta accacgaagc
      781 cgaagacctg ttctatcaaa gttcacttgc ttcttggaat tataacacca atattactga
      841 agagaatgtc caaaacatga ataatgctgg ggacaaatgg tctgcctttt taaaggaaca
      901 gtccacactt gcccaaatgt atccactaca agaaattcag aatctcacag tcaagcttca
      961 gctgcaggct cttcagcaaa atgggtcttc agtgctctca gaagacaaga gcaaacggtt
     1021 gaacacaatt ctaaatacaa tgagcaccat ctacagtact ggaaaagttt gtaacccaga
     1081 taatccacaa gaatgcttat tacttgaacc aggtttgaat gaaataatgg caaacagttt
     1141 agactacaat gagaggctct gggcttggga aagctggaga tctgaggtcg gcaagcagct
     1201 gaggccatta tatgaagagt atgtggtctt gaaaaatgag atggcaagag caaatcatta
     1261 tgaggactat ggggattatt ggagaggaga ctatgaagta aatggggtag atggctatga
     1321 ctacagccgc ggccagttga ttgaagatgt ggaacatacc tttgaagaga ttaaaccatt
     1381 atatgaacat cttcatgcct atgtgagggc aaagttgatg aatgcctatc cttcctatat
     1441 cagtccaatt ggatgcctcc ctgctcattt gcttggtgat atgtggggta gattttggac
     1501 aaatctgtac tctttgacag ttccctttgg acagaaacca aacatagatg ttactgatgc
     1561 aatggtggac caggcctggg atgcacagag aatattcaag gaggccgaga agttctttgt
     1621 atctgttggt cttcctaata tgactcaagg attctgggaa aattccatgc taacggaccc
     1681 aggaaatgtt cagaaagcag tctgccatcc cacagcttgg gacctgggga agggcgactt
     1741 caggatcctt atgtgcacaa aggtgacaat ggacgacttc ctgacagctc atcatgagat
     1801 ggggcatatc cagtatgata tggcatatgc tgcacaacct tttctgctaa gaaatggagc
     1861 taatgaagga ttccatgaag ctgttgggga aatcatgtca ctttctgcag ccacacctaa
     1921 gcatttaaaa tccattggtc ttctgtcacc cgattttcaa gaagacaatg aaacagaaat
     1981 aaacttcctg ctcaaacaag cactcacgat tgttgggact ctgccattta cttacatgtt
     2041 agagaagtgg aggtggatgg tctttaaagg ggaaattccc aaagaccagt ggatgaaaaa
     2101 gtggtgggag atgaagcgag agatagttgg ggtggtggaa cctgtgcccc atgatgaaac
     2161 atactgtgac cccgcatctc tgttccatgt ttctaatgat tactcattca ttcgatatta
     2221 cacaaggacc ctttaccaat tccagtttca agaagcactt tgtcaagcag ctaaacatga
     2281 aggccctctg cacaaatgtg acatctcaaa ctctacagaa gctggacaga aactgttcaa
     2341 tatgctgagg cttggaaaat cagaaccctg gaccctagca ttggaaaatg ttgtaggagc
     2401 aaagaacatg aatgtaaggc cactgctcaa ctactttgag cccttattta cctggctgaa
     2461 agaccagaac aagaattctt ttgtgggatg gagtaccgac tggagtccat atgcagacca
     2521 aagcatcaaa gtgaggataa gcctaaaatc agctcttgga gataaagcat atgaatggaa
     2581 cgacaatgaa atgtacctgt tccgatcatc tgttgcatat gctatgaggc agtacttttt
     2641 aaaagtaaaa aatcagatga ttctttttgg ggaggaggat gtgcgagtgg ctaatttgaa
     2701 accaagaatc tcctttaatt tctttgtcac tgcacctaaa aatgtgtctg atatcattcc
     2761 tagaactgaa gttgaaaagg ccatcaggat gtcccggagc cgtatcaatg atgctttccg
     2821 tctgaatgac aacagcctag agtttctggg gatacagcca acacttggac ctcctaacca
     2881 gccccctgtt tccatatggc tgattgtttt tggagttgtg atgggagtga tagtggttgg
     2941 cattgtcatc ctgatcttca ctgggatcag agatcggaag aagaaaaata aagcaagaag
     3001 tggagaaaat ccttatgcct ccatcgatat tagcaaagga gaaaataatc caggattcca
     3061 aaacactgat gatgttcaga cctcctttca cccagctttc ttgtacaaag ttggcattat
     3121 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3181 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     3241 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     3301 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctgcc
     3361 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     3421 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     3481 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     3541 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     3601 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3661 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3721 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3781 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3841 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3901 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3961 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4021 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     4081 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     4141 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     4201 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     4261 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     4321 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     4381 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     4441 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     4501 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4561 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4621 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4681 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4741 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4801 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4861 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4921 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     4981 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5041 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5101 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5161 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
