LOCUS pEnt223_CD147_En 3603 bp ds-DNA circular 07-APR-2020 DEFINITION synthetic circular DNA. ACCESSION . (modified) VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 3603) AUTHORS gingras TITLE Direct Submission JOURNAL Exported Apr 7, 2020 from SnapGene 4.2.4 http://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator 268..295 /note="transcription terminator T2 from the E. coli rrnB gene" /label=rrnB T2 terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 387..473 /gene="Escherichia coli rrnB" /note="transcription terminator T1 from the E. coli rrnB gene" /label=rrnB T1 terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 537..553 /note="common sequencing primer, one of multiple similar variants" /label=M13 fwd /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 569..668 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" /label=attL1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 674..1480 /created_by="User" /label=CD147 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind complement(1482..1581) /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" /label=attL2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1622..1638) /note="common sequencing primer, one of multiple similar variants" /label=M13 rev /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2069..2860 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase" /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" /label=SmR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2953..3541 /direction="RIGHT" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label=ori /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggcttc accatggcgg ctgcgctgtt cgtgctgctg ggattcgcgc tgctgggcac 721 ccacggagcc tccggggctg ccggcacagt cttcactacc gtagaagacc ttggctccaa 781 gatactcctc acctgctcct tgaatgacag cgccacagag gtcacagggc accgctggct 841 gaaggggggc gtggtgctga aggaggacgc gctgcccggc cagaaaacgg agttcaaggt 901 ggactccgac gaccagtggg gagagtactc ctgcgtcttc ctccccgagc ccatgggcac 961 ggccaacatc cagctccacg ggcctcccag agtgaaggct gtgaagtcgt cagaacacat 1021 caacgagggg gagacggcca tgctggtctg caagtcagag tccgtgccac ctgtcactga 1081 ctgggcctgg tacaagatca ctgactctga ggacaaggcc ctcatgaacg gctccgagag 1141 caggttcttc gtgagttcct cgcagggccg gtcagagcta cacattgaga acctgaacat 1201 ggaggccgac cccggccagt accggtgcaa cggcaccagc tccaagggct ccgaccaggc 1261 catcatcacg ctccgcgtgc gcagccacct ggccgccctc tggcccttcc tgggcatcgt 1321 ggctgaggtg ctggtgctgg tcaccatcat cttcatctac gagaagcgcc ggaagcccga 1381 ggacgtcctg gatgatgacg acgccggctc tgcacccctg aagagcagcg ggcagcacca 1441 gaatgacaaa ggcaagaacg tccgccagag gaactcttcc cacccagctt tcttgtacaa 1501 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag 1561 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat 1621 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc 1681 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac 1741 ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac 1801 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg 1861 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat 1921 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc 1981 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg 2041 cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac 2101 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt 2161 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct 2221 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt 2281 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt 2341 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga 2401 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct 2461 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga 2521 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac 2581 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc 2641 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg 2701 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta 2761 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca 2821 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc 2881 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat 2941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 3001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 3061 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 3121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 3181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 3241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 3301 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac 3361 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 3421 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3481 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 3541 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 3601 gtt //