LOCUS pEnt223_TMPRSS2_ 4272 bp ds-DNA circular 07-APR-2020 DEFINITION synthetic circular DNA. ACCESSION . (modified) VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 4272) AUTHORS gingras TITLE Direct Submission JOURNAL Exported Apr 7, 2020 from SnapGene 4.2.4 http://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator 268..295 /note="transcription terminator T2 from the E. coli rrnB gene" /label=rrnB T2 terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 387..473 /gene="Escherichia coli rrnB" /note="transcription terminator T1 from the E. coli rrnB gene" /label=rrnB T1 terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 537..553 /note="common sequencing primer, one of multiple similar variants" /label=M13 fwd /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 569..668 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" /label=attL1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 671..2149 /created_by="User" /label=TMPRSS2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind complement(2151..2250) /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" /label=attL2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(2291..2307) /note="common sequencing primer, one of multiple similar variants" /label=M13 rev /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2738..3529 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase" /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" /label=SmR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 3622..4210 /direction="RIGHT" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label=ori /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggcttc atggctttga actcagggtc accaccagct attggacctt actatgaaaa 721 ccatggatac caaccggaaa acccctatcc cgcacagccc actgtggtcc ccactgtcta 781 cgaggtgcat ccggctcagt actacccgtc ccccgtgccc cagtacgccc cgagggtcct 841 gacgcaggct tccaaccccg tcgtctgcac gcagcccaaa tccccatccg ggacagtgtg 901 cacctcaaag actaagaaag cactgtgcat caccttgacc ctggggacct tcctcgtggg 961 agctgcgctg gccgctggcc tactctggaa gttcatgggc agcaagtgct ccaactctgg 1021 gatagagtgc gactcctcag gtacctgcat caacccctct aactggtgtg atggcgtgtc 1081 acactgcccc ggcggggagg acgagaatcg gtgtgttcgc ctctacggac caaacttcat 1141 ccttcaggtg tactcatctc agaggaagtc ctggcaccct gtgtgccaag acgactggaa 1201 cgagaactac gggcgggcgg cctgcaggga catgggctat aagaataatt tttactctag 1261 ccaaggaata gtggatgaca gcggatccac cagctttatg aaactgaaca caagtgccgg 1321 caatgtcgat atctataaaa aactgtacca cagtgatgcc tgttcttcaa aagcagtggt 1381 ttctttacgc tgtatagcct gcggggtcaa cttgaactca agccgccaga gcaggatcgt 1441 gggcggcgag agcgcgctcc cgggggcctg gccctggcag gtcagcctgc acgtccagaa 1501 cgtccacgtg tgcggaggct ccatcatcac ccccgagtgg atcgtgacag ccgcccactg 1561 cgtggaaaaa cctcttaaca atccatggca ttggacggca tttgcgggga ttttgagaca 1621 atctttcatg ttctatggag ccggatacca agtagaaaaa gtgatttctc atccaaatta 1681 tgactccaag accaagaaca atgacattgc gctgatgaag ctgcagaagc ctctgacttt 1741 caacgaccta gtgaaaccag tgtgtctgcc caacccaggc atgatgctgc agccagaaca 1801 gctctgctgg atttccgggt ggggggccac cgaggagaaa gggaagacct cagaagtgct 1861 gaacgctgcc aaggtgcttc tcattgagac acagagatgc aacagcagat atgtctatga 1921 caacctgatc acaccagcca tgatctgtgc cggcttcctg caggggaacg tcgattcttg 1981 ccagggtgac agtggagggc ctctggtcac ttcgaagaac aatatctggt ggctgatagg 2041 ggatacaagc tggggttctg gctgtgccaa agcttacaga ccaggagtgt acgggaatgt 2101 gatggtattc acggactgga tttatcgaca aatgagggca gacggctaac acccagcttt 2161 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt 2221 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc 2281 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg 2341 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa 2401 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa 2461 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat 2521 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc 2581 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa 2641 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac 2701 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga 2761 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt 2821 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat 2881 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa 2941 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt 3001 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca 3061 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga 3121 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc 3181 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga 3241 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct 3301 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc 3361 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag 3421 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga 3481 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca 3541 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt 3601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 3781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 3901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 3961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga 4021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4261 tgctcacatg tt //