LOCUS       pEnt223_TMPRSS2_        4272 bp ds-DNA     circular     07-APR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid .
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   gingras
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2020 from SnapGene 4.2.4 http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      268..295
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
                     /label=rrnB T2 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /label=rrnB T1 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     537..553
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 fwd
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label=attL1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             671..2149
                     /created_by="User"
                     /label=TMPRSS2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    complement(2151..2250)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label=attL2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2291..2307)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2738..3529
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /label=SmR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3622..4210
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label=ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggctttga actcagggtc accaccagct attggacctt actatgaaaa
      721 ccatggatac caaccggaaa acccctatcc cgcacagccc actgtggtcc ccactgtcta
      781 cgaggtgcat ccggctcagt actacccgtc ccccgtgccc cagtacgccc cgagggtcct
      841 gacgcaggct tccaaccccg tcgtctgcac gcagcccaaa tccccatccg ggacagtgtg
      901 cacctcaaag actaagaaag cactgtgcat caccttgacc ctggggacct tcctcgtggg
      961 agctgcgctg gccgctggcc tactctggaa gttcatgggc agcaagtgct ccaactctgg
     1021 gatagagtgc gactcctcag gtacctgcat caacccctct aactggtgtg atggcgtgtc
     1081 acactgcccc ggcggggagg acgagaatcg gtgtgttcgc ctctacggac caaacttcat
     1141 ccttcaggtg tactcatctc agaggaagtc ctggcaccct gtgtgccaag acgactggaa
     1201 cgagaactac gggcgggcgg cctgcaggga catgggctat aagaataatt tttactctag
     1261 ccaaggaata gtggatgaca gcggatccac cagctttatg aaactgaaca caagtgccgg
     1321 caatgtcgat atctataaaa aactgtacca cagtgatgcc tgttcttcaa aagcagtggt
     1381 ttctttacgc tgtatagcct gcggggtcaa cttgaactca agccgccaga gcaggatcgt
     1441 gggcggcgag agcgcgctcc cgggggcctg gccctggcag gtcagcctgc acgtccagaa
     1501 cgtccacgtg tgcggaggct ccatcatcac ccccgagtgg atcgtgacag ccgcccactg
     1561 cgtggaaaaa cctcttaaca atccatggca ttggacggca tttgcgggga ttttgagaca
     1621 atctttcatg ttctatggag ccggatacca agtagaaaaa gtgatttctc atccaaatta
     1681 tgactccaag accaagaaca atgacattgc gctgatgaag ctgcagaagc ctctgacttt
     1741 caacgaccta gtgaaaccag tgtgtctgcc caacccaggc atgatgctgc agccagaaca
     1801 gctctgctgg atttccgggt ggggggccac cgaggagaaa gggaagacct cagaagtgct
     1861 gaacgctgcc aaggtgcttc tcattgagac acagagatgc aacagcagat atgtctatga
     1921 caacctgatc acaccagcca tgatctgtgc cggcttcctg caggggaacg tcgattcttg
     1981 ccagggtgac agtggagggc ctctggtcac ttcgaagaac aatatctggt ggctgatagg
     2041 ggatacaagc tggggttctg gctgtgccaa agcttacaga ccaggagtgt acgggaatgt
     2101 gatggtattc acggactgga tttatcgaca aatgagggca gacggctaac acccagcttt
     2161 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2221 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2281 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2341 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2401 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2461 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2521 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2581 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2641 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2701 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     2761 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2821 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     2881 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2941 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3001 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3061 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3121 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3181 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3241 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3301 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3361 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3421 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3481 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     3541 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     4021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4261 tgctcacatg tt    
//
