LOCUS       Exported               11269 bp ds-DNA     circular SYN 08-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Baisong Lu
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 1, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             71..304
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
     polyA_signal    376..497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      537..672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(721..737)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      879..1334
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1360..1464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1465..2325
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2496..3084
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3451..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3486..3516
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3631..3857
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3858..4038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4085..4210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4703..4936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        5511..5714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5791..6636
                     /codon_start=1
                     /label=ATF5
                     /translation="MSLLATLGLELDRALLPASGLGWLVDYGKLPPAPAPLAPYEVLGG
                     ALEGGLPVGGEPLAGDGFSDWMTERVDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASL
                     LKKELEQMEDFFLDAPPLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLA
                     IYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAA
                     LRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRS
                     C"
     CDS             6646..6702
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6703..8913
                     /codon_start=1
                     /label=PROX1
                     /translation="MPDHDSTALLSRQTKRRRVDIGVKRTVGTASAFFAKARATFFSAM
                     NPQGSEQDVEYSVVQHADGEKSNVLRKLLKRANSYEDAMMPFPGATIISQLLKNNMNKN
                     GGTEPSFQASGLSSTGSEVHQEDICSNSSRDSPPECLSPFGRPTMSQFDMDRLCDEHLR
                     AKRARVENIIRGMSHSPSVALRGNENEREMAPQSVSPRESYRENKRKQKLPQQQQQSFQ
                     QLVSARKEQKREERRQLKQQLEDMQKQLRQLQEKFYQIYDSTDSENDEDGNLSEDSMRS
                     EILDARAQDSVGRSDNEMCELDPGQFIDRARALIREQEMAENKPKREGNNKERDHGPNS
                     LQPEGKHLAETLKQELNTAMSQVVDTVVKVFSAKPSRQVPQVFPPLQIPQARFAVNGEN
                     HNFHTANQRLQCFGDVIIPNPLDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAG
                     GHHQPLHQSPLSATTGFTTSTFRHPFPLPLMAYPFQSPLGAPSGSFSGKDRASPESLDL
                     TRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECGDLQDMSEISPYSGSAM
                     QEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQM
                     EKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFN
                     AIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE"
     CDS             8914..8967
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8968..10884
                     /codon_start=1
                     /label=HNF1A
                     /translation="MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLD
                     KGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVV
                     ETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALY
                     TWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQK
                     NPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKL
                     AMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGP
                     LVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQ
                     PQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTT
                     LQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGL
                     LPQTMLITDTTNLSALASLTPTKQEAALLPQVFTSDTEASSESGLHTPASQATTLHVPS
                     QDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMAS
                     SSQ"
     misc_feature    10885..11002
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     11002..11023
                     /label=5in1-loopF
     misc_feature    11003..11134
                     /label=HBB 3' UTR
                     /note="HBB 3' UTR"
     primer_bind     11003..11023
                     /label=4in1-loopF
     misc_feature    11137..11268
                     /label=HBB 3' UTR
                     /note="HBB 3' UTR"
     primer_bind     11137..11157
                     /label=4in1-loopF
     primer_bind     11137..11157
                     /label=5in1-loopF
ORIGIN
        1 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
       61 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
      121 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      181 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      241 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      301 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
      361 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      421 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      481 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
      541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
      661 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
      721 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      781 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      841 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
      901 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      961 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1021 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1081 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1141 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1201 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1261 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1321 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1381 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1441 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1501 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1561 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1621 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1681 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1741 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1801 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1861 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1921 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1981 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2041 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2101 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2161 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2221 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2281 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2341 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2401 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2641 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2881 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3061 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3181 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3241 gagtcagtga gcgaggaagc ggaagagcgc cctttgagtg agctgatacc gctcgccgca
     3301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3421 actggaaagc gggcagtgag cgcaacgcaa aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     3661 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     3721 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     3781 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     3841 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4021 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     4081 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     4141 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     4201 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     4261 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     4321 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     4381 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     4441 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     4501 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     4561 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     4621 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     4681 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     4741 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     4801 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     4861 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     4921 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     4981 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     5041 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     5101 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     5161 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     5221 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     5281 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     5341 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     5401 tagtgaacgg atctcgacgg tatcgatact agtattatgc ccagtacatg accttatggg
     5461 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     5521 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     5581 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     5641 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     5701 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     5761 ttgacctcca tagaagattc tagagccacc atgtcactcc tggcgaccct ggggctggag
     5821 ctggacaggg ccctgctccc agctagtggg ctgggatggc tcgtagacta tgggaaactc
     5881 cccccggccc ctgcccccct ggctccctat gaggtccttg ggggagccct ggagggcggg
     5941 cttccagtgg ggggagagcc cctggcaggt gatggcttct ctgactggat gactgagcga
     6001 gttgatttca cagctctcct ccctctggag cctcccttac cccccggcac cctcccccaa
     6061 ccttccccaa ccccacctga cctggaagct atggcctccc tcctcaagaa ggagctggaa
     6121 cagatggaag acttcttcct agatgccccg cccctcccac caccctcccc gccgccacta
     6181 ccaccaccac cactaccacc agccccctcc ctccccctgt ccctcccctc ctttgacctc
     6241 ccccagcccc ctgtcttgga tactctggac ttgctggcca tctactgccg caacgaggcc
     6301 gggcaggagg aagtggggat gccgcctctg cccccgccac agcagccccc tcctccttct
     6361 ccacctcaac cttctcgcct ggccccctac ccacatcctg ccaccacccg aggggaccgc
     6421 aagcaaaaga agagagacca gaacaagtcg gcggctctga ggtatcgcca gcggaagcgg
     6481 gcagagggtg aggccctgga gggcgagtgc caggggctgg aggcacggaa tcgcgagctg
     6541 aaggaacggg cagagtccgt ggagcgcgag atccagtacg tcaaggacct gctcatcgag
     6601 gtttacaagg cccggagcca gaggacccgt agctgcggca gcggcgccac aaacttctct
     6661 ctgctaaagc aagcaggtga tgttgaagaa aaccccgggc ctatgcctga ccatgacagc
     6721 acagccctct taagccggca aaccaagagg agaagagttg acattggagt gaaaaggacg
     6781 gtagggacag catctgcatt ttttgctaag gcaagagcaa cgttttttag tgccatgaat
     6841 ccccaaggtt ctgagcagga tgttgagtat tcagtggtgc agcatgcaga tggggaaaag
     6901 tcaaatgtac tccgcaagct gctgaagagg gcgaactcgt atgaagatgc catgatgcct
     6961 tttccaggag caaccataat ttcccagctg ttgaaaaata acatgaacaa aaatggtggc
     7021 acggagccca gtttccaagc cagcggtctc tctagtacag gctccgaagt acatcaggag
     7081 gatatatgca gcaactcttc aagagacagc cccccagagt gtctttcccc ttttggcagg
     7141 cctactatga gccagtttga tatggatcgc ttatgtgatg agcacctgag agcaaagcgc
     7201 gcccgggttg agaatataat tcggggtatg agccattccc ccagtgtggc attaaggggc
     7261 aatgaaaatg aaagagagat ggccccgcag tctgtgagtc cccgagaaag ttacagagaa
     7321 aacaaacgca agcaaaagct tccccagcag cagcaacaga gtttccagca gctggtttca
     7381 gcccgaaaag aacagaagcg agaggagcgc cgacagctga aacagcagct ggaggacatg
     7441 cagaaacagc tgcgccagct gcaggaaaag ttctaccaaa tctatgacag cactgattcg
     7501 gaaaatgatg aagatggtaa cctgtctgaa gacagcatgc gctcggagat cctggatgcc
     7561 agggcccagg actctgtcgg aaggtcagat aatgagatgt gcgagctaga cccaggacag
     7621 tttattgacc gagctcgagc cctgatcaga gagcaggaaa tggctgaaaa caagccgaag
     7681 cgagaaggca acaacaaaga aagagaccat gggccaaact ccttacaacc ggaaggcaaa
     7741 catttggctg agaccttgaa acaggaactg aacactgcca tgtcgcaagt tgtggacact
     7801 gtggtcaaag tcttttcggc caagccctcc cgccaggttc ctcaggtctt cccacctctc
     7861 cagatccccc aggccagatt tgcagtcaat ggggaaaacc acaatttcca caccgccaac
     7921 cagcgcctgc agtgctttgg cgacgtcatc attccgaacc ccctggacac ctttggcaat
     7981 gtgcagatgg ccagttccac tgaccagaca gaagcactgc ccctggttgt ccgcaaaaac
     8041 tcctctgacc agtctgcctc cggccctgcc gctggcggcc accaccagcc cctgcaccag
     8101 tcgcctctct ctgccaccac gggcttcacc acgtccacct tccgccaccc cttccccctt
     8161 cccttgatgg cctatccatt tcagagccca ttaggtgctc cctccggctc cttctctgga
     8221 aaagacagag cctctcctga atccttagac ttaactaggg ataccacgag tctgaggacc
     8281 aagatgtcat ctcaccacct gagccaccac ccttgttcac cagcacaccc gcccagcacc
     8341 gccgaagggc tctccttgtc gctcataaag tccgagtgcg gcgatcttca agatatgtct
     8401 gaaatatcac cttattcggg aagtgcaatg caggaaggat tgtcacccaa tcacttgaaa
     8461 aaagcaaagc tcatgttttt ttatacccgt tatcccagct ccaatatgct gaagacctac
     8521 ttctccgacg taaagttcaa cagatgcatt acctctcagc tcatcaagtg gtttagcaat
     8581 ttccgtgagt tttactacat tcagatggag aagtacgcac gtcaagccat caacgatggg
     8641 gtcaccagta ctgaagagct gtctataacc agagactgtg agctgtacag ggctctgaac
     8701 atgcactaca ataaagcaaa tgactttgag gttccagaga gattcctgga agttgctcag
     8761 atcacattac gggagttttt caatgccatt atcgcaggca aagatgttga tccttcctgg
     8821 aagaaggcca tatacaaggt catctgcaag ctggatagtg aagtccctga gattttcaaa
     8881 tccccgaact gcctacaaga gctgcttcat gaggagggca gaggaagtct tctaacatgc
     8941 ggtgacgtgg aggagaatcc cggccctatg gtttctaaac tgagccagct gcagacggag
     9001 ctcctggcgg ccctgctcga gtcagggctg agcaaagagg cactgatcca ggcactgggt
     9061 gagccggggc cctacctcct ggctggagaa ggccccctgg acaaggggga gtcctgcggc
     9121 ggcggtcgag gggagctggc tgagctgccc aatgggctgg gggagactcg gggctccgag
     9181 gacgagacgg acgacgatgg ggaagacttc acgccaccca tcctcaaaga gctggagaac
     9241 ctcagccctg aggaggcggc ccaccagaaa gccgtggtgg agacccttct gcaggaggac
     9301 ccgtggcgtg tggcgaagat ggtcaagtcc tacctgcagc agcacaacat cccacagcgg
     9361 gaggtggtcg ataccactgg cctcaaccag tcccacctgt cccaacacct caacaagggc
     9421 actcccatga agacgcagaa gcgggccgcc ctgtacacct ggtacgtccg caagcagcga
     9481 gaggtggcgc agcagttcac ccatgcaggg cagggagggc tgattgaaga gcccacaggt
     9541 gatgagctac caaccaagaa ggggcggagg aaccgtttca agtggggccc agcatcccag
     9601 cagatcctgt tccaggccta tgagaggcag aagaacccta gcaaggagga gcgagagacg
     9661 ctagtggagg agtgcaatag ggcggaatgc atccagagag gggtgtcccc atcacaggca
     9721 caggggctgg gctccaacct cgtcacggag gtgcgtgtct acaactggtt tgccaaccgg
     9781 cgcaaagaag aagccttccg gcacaagctg gccatggaca cgtacagcgg gcccccccca
     9841 gggccaggcc cgggacctgc gctgcccgct cacagctccc ctggcctgcc tccacctgcc
     9901 ctctccccca gtaaggtcca cggtgtgcgc tatggacagc ctgcgaccag tgagactgca
     9961 gaagtaccct caagcagcgg cggtccctta gtgacagtgt ctacacccct ccaccaagtg
    10021 tcccccacgg gcctggagcc cagccacagc ctgctgagta cagaagccaa gctggtctca
    10081 gcagctgggg gccccctccc ccctgtcagc accctgacag cactgcacag cttggagcag
    10141 acatccccag gcctcaacca gcagccccag aacctcatca tggcctcact tcctggggtc
    10201 atgaccatcg ggcctggtga gcctgcctcc ctgggtccta cgttcaccaa cacaggtgcc
    10261 tccaccctgg tcatcggcct ggcctccacg caggcacaga gtgtgccggt catcaacagc
    10321 atgggcagca gcctgaccac cctgcagccc gtccagttct cccagccgct gcacccctcc
    10381 taccagcagc cgctcatgcc acctgtgcag agccatgtga cccagagccc cttcatggcc
    10441 accatggctc agctgcagag cccccacgcc ctctacagcc acaagcccga ggtggcccag
    10501 tacacccaca cgggcctgct cccgcagact atgctcatca ccgacaccac caacctgagc
    10561 gccctggcca gcctcacgcc caccaagcag gaggctgctc tgctccccca ggtcttcacc
    10621 tcagacactg aggcctccag tgagtccggg cttcacacgc cggcatctca ggccaccacc
    10681 ctccacgtcc ccagccagga ccctgccggc atccagcacc tgcagccggc ccaccggctc
    10741 agcgccagcc ccacagtgtc ctccagcagc ctggtgctgt accagagctc agactccagc
    10801 aatggccaga gccacctgct gccatccaac cacagcgtca tcgagacctt catctccacc
    10861 cagatggcct cttcctccca gtaattttaa aagaaaaggg gggattgggg ggtacagtgc
    10921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    10981 aattacaaaa attcaaaatt ttgctcgctt tcttgctgtc caatttctat taaaggttcc
    11041 tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt gagcatctgg
    11101 attctgccta ataaaaaaca tttattttca ttgctagctc gctttcttgc tgtccaattt
    11161 ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata ttatgaaggg
    11221 ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcg
//
