LOCUS       PB-CAG-mRuby-Gal8-P2A-Z 7864 bp ds-DNA     circular     01-AUG-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     feature         complement(314..347)
                     /label="loxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5253..5287
                     /label="5' ITR specific sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2041..2060
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(5359..5388)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        5494..5673
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(7718..7746)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     regulatory      2126..2135
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      4397..4898
                     /label="rb_glob_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            complement(6816..7676)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3929..3995
                     /label="P2A"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2852..3928
                     /label="Gal8"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    2843..2851
                     /label="Linker"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     primer          5775..5794
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(5306..5324)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    381..2082
                     /label="CAG Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     regulatory      447..734
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     origin          complement(6042..6661)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5323..5345)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          5529..5606
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    join(7815..7864,1..380)
                     /label="3' Terminal Repeat"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      5687..5818
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5591..5610
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4917..5292
                     /label="5' Terminal Repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    join(7819..7864,1..17)
                     /label="3' ITR specific sequence"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3998..4372
                     /label="Sh Ble Zeo resistance"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     ORF             2132..2842
                     /label="mRuby3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 CGTAAAATTG ACGCATGTGT TTTATCGATC TGTATATCGA GGTTTATTTA TTAATTTGAA
       61 TAGATATTAA GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT
      121 TATTTATGTT TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA
      181 ACAAAACTTT TAAACATTCT CTCTTTTACA AAAATAAACT TATTTTGTAC TTTAAAAACA
      241 GTCATGTTGT ATTATAAAAT AAGTAATTAG CTTAACTTAT ACATAATAGA AACAAATTAT
      301 ACTTATTAAT CGCATAACTT CGTATAATGT ATGCTATACG AAGTTATGCG ATTAAGGGAT
      361 CTGTAGGGCG CAGTAGTCCA ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC
      421 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC
      481 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
      541 TAGGGACTTT CCATTGACGT CAATGGGTGG ACTATTTACG GTAAACTGCC CACTTGGCAG
      601 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
      661 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
      721 ACGTATTAGT CATCGCTATT ACCATGGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC
      781 CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT
      841 GCAGCGATGG GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG
      901 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA
      961 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG
     1021 GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
     1081 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
     1141 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTCGTT TCTTTTCTGT GGCTGCGTGA
     1201 AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG GAGCGGCTCG GGGGGTGCGT
     1261 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCCCGCG CTGCCCGGCG GCTGTGAGCG
     1321 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCGTGTGC GCGAGGGGAG CGCGGCCGGG
     1381 GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG CGGGGTGTGT
     1441 GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGGCGGTCGG GCTGTAACCC CCCCCTGCAC
     1501 CCCCCTCCCC GAGTTGCTGA GCACGGCCCG GCTTCGGGTG CGGGGCTCCG TGCGGGGCGT
     1561 GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG
     1621 GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG GAGGGGCGCG GCGGCCCCGG AGCGCCGGCG
     1681 GCTGTCGAGG CGCGGCGAGC CGCAGCCATT GCCTTTTATG GTAATCGTGC GAGAGGGCGC
     1741 AGGGACTTCC TTTGTCCCAA ATCTGGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC
     1801 CTCTAGCGGG CGCGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC
     1861 CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCATCTCC AGCCTCGGGG CTGCCGCAGG
     1921 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG
     1981 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
     2041 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT
     2101 ACCGCGGGCC CGGGATCCAC CGGTCgccac catggtgtct aagggcgaag agctgatcaa
     2161 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
     2221 cacaggtgaa ggagaaggca gaccgtacga gggagtgcaa accatgagga tcaaagtcat
     2281 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
     2341 ccgtaccttt atcaagtacc cggccgacat ccctgatttc tttaaacagt cctttcctga
     2401 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcacgca
     2461 ggacaccagc cttgaggatg gcgagctcgt ctacaacgtc aaggtcagag gggtaaactt
     2521 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
     2581 gatgtatcca gcagatggtg gtctgagagg atacactgac atcgcactga aagttgatgg
     2641 tggtggccat ctgcactgca acttcgtgac aacttacagg tcaaaaaaga ccgtcgggaa
     2701 catcaagatg cccggtgtcc atgccgttga tcaccgcctg gaaaggatcg aggagagtga
     2761 caatgaaacc tacgtagtgc aaagagaagt ggcagttgcc aaatacagca accttggtgg
     2821 tggcatggac gagctgtaca agGACATGTC CATGATGTTG TCCTTAAACA ACCTACAGAA
     2881 TATCATCTAT AACCCGGTAA TCCCGTTTGT TGGCACCATT CCTGATCAGC TGGATCCTGG
     2941 AACTTTGATT GTGATACGTG GGCATGTTCC TAGTGACGCA GACAGATTCC AGGTGGATCT
     3001 GCAGAATGGC AGCAGCATGA AACCTCGAGC CGATGTGGCC TTTCATTTCA ATCCTCGTTT
     3061 CAAAAGGGCC GGCTGCATTG TTTGCAATAC TTTGATAAAT GAAAAATGGG GACGGGAAGA
     3121 GATCACCTAT GACACGCCTT TCAAAAGAGA AAAGTCTTTT GAGATCGTGA TTATGGTGCT
     3181 GAAGGACAAA TTCCAGGTGG CTGTAAATGG AAAACATACT CTGCTCTATG GCCACAGGAT
     3241 CGGCCCAGAG AAAATAGACA CTCTGGGCAT TTATGGCAAA GTGAATATTC ACTCAATTGG
     3301 TTTTAGCTTC AGCTCGGACT TACAAAGTAC CCAAGCATCT AGTCTGGAAC TGACAGAGAT
     3361 AAGTAGAGAA AATGTTCCAA AGTCTGGCAC GCCCCAGCTT CCTAGTAATA GAGGAGGAGA
     3421 CATTTCTAAA ATCGCACCCA GAACTGTCTA CACCAAGAGC AAAGATTCGA CTGTCAATCA
     3481 CACTTTGACT TGCACCAAAA TACCACCTAT GAACTATGTG TCAAAGAGGC TGCCATTCGC
     3541 TGCAAGGTTG AACACCCCCA TGGGCCCTGG ACGAACTGTC GTCGTTAAAG GAGAAGTGAA
     3601 TGCAAATGCC AAAAGCTTTA ATGTTGACCT ACTAGCAGGA AAATCAAAGG ATATTGCTCT
     3661 ACACTTGAAC CCACGCCTGA ATATTAAAGC ATTTGTAAGA AATTCTTTTC TTCAGGAGTC
     3721 CTGGGGAGAA GAAGAGAGAA ATATTACCTC TTTCCCATTT AGTCCTGGGA TGTACTTTGA
     3781 GATGATAATT TATTGTGATG TTAGAGAATT CAAGGTTGCA GTAAATGGCG TACACAGCCT
     3841 GGAGTACAAA CACAGATTTA AAGAGCTCAG CAGTATTGAC ACGCTGGAAA TTAATGGAGA
     3901 CATCCACTTA CTGGAAGTAA GGAGCTGGgg aagcggagct actaacttca gcctgctgaa
     3961 gcaggctgga gacgtggagg agaaccctgg acctataATG GCCAAGTTGA CCAGTGCTGT
     4021 CCCAGTGCTC ACAGCCAGGG ATGTGGCTGG AGCTGTTGAG TTCTGGACTG ACAGGTTGGG
     4081 GTTCTCCAGA GATTTTGTGG AGGATGACTT TGCAGGTGTG GTCAGAGATG ATGTCACCCT
     4141 GTTCATCTCA GCAGTCCAGG ACCAGGTGGT GCCTGACAAC ACCCTGGCTT GGGTGTGGGT
     4201 GAGAGGACTG GATGAGCTGT ATGCTGAGTG GAGTGAGGTG GTCTCCACCA ACTTCAGGGA
     4261 TGCCAGTGGC CCTGCCATGA CAGAGATTGG AGAGCAGCCC TGGGGGAGAG AGTTTGCCCT
     4321 GAGAGACCCA GCAGGCAACT GTGTGCACTT TGTGGCAGAG GAGCAGGACT GAttctagaG
     4381 CGGCCGCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG TGGCCAATGC
     4441 CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT
     4501 GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT
     4561 GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA
     4621 ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCATATGCT GGCTGCCATG
     4681 AACAAAGGTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT
     4741 TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT
     4801 TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC
     4861 TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGAAGATCC CTCGACCTGC
     4921 AGGCTCCAGC CTACACAATC GCTCAAGACG TGTAATGCTT TTATTATATA TTAGTCACGA
     4981 TATCTATAAC AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC
     5041 AATATAATTA TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT
     5101 TTGACTCACG CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC
     5161 CTCACGGGAG CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT
     5221 ATTTAGAAAG AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT
     5281 TCTAGGGTTA AAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     5341 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     5401 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     5461 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
     5521 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
     5581 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
     5641 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
     5701 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
     5761 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
     5821 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
     5881 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
     5941 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
     6001 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
     6061 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
     6121 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
     6181 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
     6241 GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
     6301 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
     6361 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
     6421 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
     6481 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
     6541 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     6601 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
     6661 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     6721 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     6781 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     6841 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     6901 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC
     6961 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     7021 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     7081 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     7141 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     7201 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     7261 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     7321 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     7381 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     7441 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     7501 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     7561 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     7621 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     7681 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     7741 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
     7801 CCACCTGGGT CGACTTAACC CTAGAAAGAT AATCATATTG TGACGTACGT TAAAGATAAT
     7861 CATG
//