LOCUS       MA111-p2T-CMV-CDAmax-BlastR       10413 bp ds-DNA     circular     05-OCT-2018
DEFINITION  CDA.
ACCESSION   urn.local...g-bv9vqqj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        join(10359..10413,1..27)
                     /label="CMV prom"
     promoter        join(9852..10413,1..27)
                     /label="CMV_Full"
     misc_feature    40..47
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     primer_bind     48..67
                     /label="T7 promoter"
     misc_feature    81..137
                     /label="Bipartite SV40 NLS"
     misc_feature    138..140
                     /label="5' flanking"
     misc_feature    138..140
                     /label="Ser"
     misc_feature    141..761
                     /label="CDA"
     misc_feature    271..278
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    272..277
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(470..476)
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    489..499
                     /label="USER jct 30"
     misc_feature    702..709
                     /label="USER jct 22"
     CDS             762..767
                     /label="Genscript BE4 linker"
     misc_feature    765..767
                     /label="3' GGC"
     CDS             768..857
                     /label="Genscript BE4 linker"
     misc_feature    773..776
                     /label="TAGC"
     misc_feature    774..783
                     /label="USER jct 33"
     CDS             858..4958
                     /label="fz hSpCas9"
     misc_feature    882..884
                     /label="D10A"
     misc_feature    3090..3095
                     /Recognition_pattern="GAG^CTC"
                     /annotation_group="GAGCTC"
                     /label="Ecl136II, Eco53kI, EcoICRI"
                     /note="Geneious type: restriction site"
     misc_feature    3090..3095
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, Psp124BI, SstI"
                     /note="Geneious type: restriction site"
     source          4959..5528
                     /label="genscript 2xUGI"
     misc_feature    5061..5067
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    5407..5417
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    5529..5558
                     /label="SV40 NLS"
     misc_feature    5559..5579
                     /label="SV40 NLS"
     misc_feature    5582..5587
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    5609..5616
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     misc_feature    5626..5629
                     /label="BsaI AGCC"
     Terminator      5631..5858
                     /label="bGH_PA term"
     misc_feature    complement(5947..5974)
                     /label="lacO reg"
     protein_bind    5953..5969
                     /label="lac operator"
     promoter        complement(5977..6007)
                     /label="lac promoter"
     protein_bind    6022..6043
                     /label="CAP binding site"
     misc_feature    6150..7292
                     /label="BlastR cassette"
     polya_signal    6160..6281
                     /label="SV40 poly(A) signal"
     misc_feature    complement(6207..6281)
                     /label="SV40 late terminator"
     misc_feature    6259..6264
                     /Recognition_pattern="TTA^TAA"
                     /annotation_group="TTATAA"
                     /label="PsiI, AanI"
                     /note="Geneious type: restriction site"
     gene            complement(6439..6837)
                     /label="BlastR Gene"
     misc_feature    complement(6838..6903)
                     /label="EM7 promoter"
     misc_feature    6940..6945
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    6940..6945
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     promoter        complement(6951..7280)
                     /label="SV40 promoter"
     misc_feature    6964..6969
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI, Eco147I, PceI, SseBI"
                     /note="Geneious type: restriction site"
     rep_origin      6965..7100
                     /label="SV40 ori"
     misc_feature    complement(6976..7051)
                     /label="SV40 ORI"
     misc_feature    complement(7008..7289)
                     /label="SV40 early promoter"
     misc_feature    7012..7022
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     user            complement(7126..7151)
                     /label="350_010818_BlastR_FwU"
     primer_bind     complement(7128..7152)
                     /label="ACK18"
     misc_feature    7193..7199
                     /Recognition_pattern="A^CCWGGT"
                     /annotation_group="ACCWGGT"
                     /label="SexAI, CsiI, MabI"
                     /note="Geneious type: restriction site"
     user            7388..7427
                     /created_by="User"
                     /modified_by="User"
                     /label="071718_Tol5'_PciI_InF_Fw"
     misc_feature    7398..7403
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     LTR             7404..7603
                     /created_by="User"
                     /label="Tol-5'"
     misc_feature    7556..7561
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII, BfrI, BspTI, BstAFI, MspCI, Vha464I"
                     /note="Geneious type: restriction site"
     user            complement(7583..7618)
                     /created_by="User"
                     /modified_by="User"
                     /label="071718_Tol5'_InF_Rv"
     rep_origin      7646..8326
                     /label="pUC origin"
     misc_feature    8286
                     /label="G>A"
     misc_feature    8316..8326
                     /label="USER jct 25"
     Marker          complement(8423..9283)
                     /label="bla (AmpR)"
     misc_feature    8563..8568
                     /label="removed BsaI site"
     misc_feature    8566..8574
                     /label="USER jct 31"
     misc_feature    8716..8721
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    9298..9303
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     Promoter        complement(9325..9353)
                     /label="amp prom"
     misc_feature    9416..9423
                     /Recognition_pattern="CG^TCGACG"
                     /annotation_group="CGTCGACG"
                     /label="SgrDI"
                     /note="Geneious type: restriction site"
     misc_feature    9433..9438
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
                     /note="Geneious type: restriction site"
     user            9641..9673
                     /created_by="User"
                     /modified_by="User"
                     /label="071718_Tol3'_InF_Fw"
     LTR             9656..9828
                     /created_by="User"
                     /label="Tol-3'"
     misc_feature    9656..9661
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    9656..9661
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    9664..9669
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     user            complement(9799..9844)
                     /created_by="User"
                     /modified_by="User"
                     /label="071718_Tol3'_MluI_InF_Rv"
     misc_feature    9829..9834
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     misc_feature    9850..9855
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    10041
                     /label="C"
     misc_feature    10084..10089
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    10189..10194
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca atgaaacgga cagccgacgg aagcgagttc gagtcaccaa
      121 agaagaagcg gaaagtcagt accgacgccg agtacgtgcg gatccacgag aagctggata
      181 tctacacatt caagaagcag ttctttaaca acaagaagtc cgtgtctcac agatgctacg
      241 tgctgttcga gctgaagcgg agaggagaga ggcgcgcctg tttttggggc tatgccgtga
      301 acaagccaca gtctggaacc gagaggggaa tccacgcaga gatcttcagc atcaggaagg
      361 tggaggagta cctgcgcgac aaccccggcc agtttacaat caattggtat agctcctgga
      421 gcccttgcgc cgattgtgcc gagaagatcc tggagtggta caaccaggag ctgaggggca
      481 atggccacac cctgaagatc tgggcctgca agctgtacta tgagaagaac gccaggaatc
      541 agatcggcct gtggaacctg cgcgacaatg gcgtgggcct gaacgtgatg gtgtccgagc
      601 actatcagtg ctgtcgcaag atctttatcc agtctagcca caatcagctg aacgagaatc
      661 ggtggctgga gaagacactg aagagagccg agaagcggag aagcgagctg tccatcatga
      721 tccaggtgaa gatcctgcac accacaaagt ctcccgccgt gtctggcgga tctagcggag
      781 gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc aacaccagag agcagtggcg
      841 gcagcagcgg cggcagcgac aagaagtaca gcatcggcct ggccatcggc accaactctg
      901 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
      961 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
     1021 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
     1081 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
     1141 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
     1201 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
     1261 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
     1321 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
     1381 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
     1441 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
     1501 tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg
     1561 gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact
     1621 tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg
     1681 acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc
     1741 tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc
     1801 ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga
     1861 aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca
     1921 agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca
     1981 tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag
     2041 aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc
     2101 tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca
     2161 accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg
     2221 ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct
     2281 ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga
     2341 ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
     2401 agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa
     2461 agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca
     2521 accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg
     2581 actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg
     2641 atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc
     2701 tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc
     2761 tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat
     2821 acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg
     2881 gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc
     2941 tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc
     3001 agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg
     3061 gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc
     3121 ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga
     3181 acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc
     3241 tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc
     3301 tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact
     3361 acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg
     3421 tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg
     3481 tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa
     3541 agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct
     3601 tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg
     3661 actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga
     3721 tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc
     3781 gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg
     3841 ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt
     3901 acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt
     3961 acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg
     4021 agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata
     4081 agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga
     4141 aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca
     4201 gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca
     4261 gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga
     4321 aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga
     4381 agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca
     4441 tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct
     4501 ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc
     4561 tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac
     4621 agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt
     4681 tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca
     4741 agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga
     4801 ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt
     4861 acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt
     4921 acgagacacg gatcgacctg tctcagctgg gaggtgacag cggcgggagc ggcgggagcg
     4981 gggggagcac taatctgagc gacatcattg agaaggagac tgggaaacag ctggtcattc
     5041 aggagtccat cctgatgctg cctgaggagg tggaggaagt gatcggcaac aagccagagt
     5101 ctgacatcct ggtgcacacc gcctacgacg agtccacaga tgagaatgtg atgctgctga
     5161 cctctgacgc ccccgagtat aagccttggg ccctggtcat ccaggattct aacggcgaga
     5221 ataagatcaa gatgctgagc ggaggatccg gaggatctgg aggcagcacc aacctgtctg
     5281 acatcatcga gaaggagaca ggcaagcagc tggtcatcca ggagagcatc ctgatgctgc
     5341 ccgaagaagt cgaagaagtg atcggaaaca agcctgagag cgatatcctg gtccataccg
     5401 cctacgacga gagtaccgac gaaaatgtga tgctgctgac atccgacgcc ccagagtata
     5461 agccctgggc tctggtcatc caggattcca acggagagaa caaaatcaaa atgctgtctg
     5521 gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct
     5581 aaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     5641 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5701 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5761 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5821 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5881 ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5941 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6001 gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6061 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6121 ggggagaggc ggtttgcgta ttgggcgcta cagacatgat aagatacatt gatgagtttg
     6181 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     6241 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
     6301 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     6361 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc
     6421 tcctcggcca cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat
     6481 cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag
     6541 atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc
     6601 gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac
     6661 gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc
     6721 gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat
     6781 tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt
     6841 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     6901 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     6961 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     7021 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     7081 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     7141 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     7201 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7261 ccacacccta actgacacac attccacaga attcactgac tcgctgcgct cggtcgttcg
     7321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7381 ggataacgca ggaaagaaca tgtaagtgat ctccaaaaaa taagtacttt ttgactgtaa
     7441 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
     7501 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
     7561 gtacagtaat caagtaaaat tactcaagta ctttacacct ctgcatgtga gcaaaaggcc
     7621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8101 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8281 ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8341 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8401 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8461 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8521 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     8581 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8641 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8701 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8761 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8821 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8881 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8941 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9001 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9061 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9121 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9181 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9241 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9301 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9361 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9421 acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg
     9481 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9541 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9601 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgggtac
     9661 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
     9721 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
     9781 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaac gcgttgacat
     9841 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9901 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9961 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    10021 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    10081 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    10141 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    10201 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    10261 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    10321 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    10381 ggtaggcgtg tacggtggga ggtctatata agc
//
