LOCUS       MA113-p2T-CMV-eA3Amax-BlastR       10383 bp ds-DNA     circular     05-OCT-2018
DEFINITION  eAPOBEC3A.
ACCESSION   urn.local...1z-bva12ch
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        join(10329..10383,1..27)
                     /note="Geneious type: Promoter"
     promoter        join(9822..10383,1..27)
     primer_bind     48..67
                     /standard_name="T7 promoter"
     misc_feature    81..5552
                     /note="Geneious type: ORF"
                     /standard_name="ORF 1 (frame 3)"
     misc_feature    81..137
                     /standard_name="Bipartite SV40 NLS"
     misc_feature    complement(117..136)
                     /note="Geneious type: user"
                     /standard_name="092518_p2TblastRmax_NLS_RvU"
     gene            126..753
                     /standard_name="Ordered from IDT as Gblock"
     misc_feature    126..136
                     /note="Geneious type: user"
                     /standard_name="USER junction"
     misc_feature    126..143
                     /note="Geneious type: user"
                     /standard_name="092518_eA3A_FwU-GS"
     CDS             138..731
                     /standard_name="eA3A N57G - GenScript codon optimization"
     misc_feature    303..305
                     /note="Geneious type: user"
                     /standard_name="N57G"
     CDS             732..827
                     /standard_name="Genscript BE4 linker"
     misc_feature    complement(736..753)
                     /note="Geneious type: user"
                     /standard_name="092518_eA3A_RvU"
     misc_feature    740..758
                     /note="Geneious type: user"
                     /standard_name="092518_p2TblastRmax_GGS_FwU"
     misc_feature    740..753
                     /note="Geneious type: user"
                     /standard_name="User junction"
     CDS             828..4928
                     /standard_name="fz hSpCas9"
     misc_feature    852..854
                     /standard_name="D10A"
     source          4929..5498
     misc_feature    5499..5528
                     /standard_name="SV40 NLS"
     misc_feature    5529..5549
                     /standard_name="SV40 NLS"
     misc_feature    5596..5599
                     /standard_name="BsaI AGCC"
     terminator      5601..5828
                     /note="Geneious type: Terminator"
     misc_feature    complement(5917..5944)
                     /standard_name="lacO reg"
     protein_bind    5923..5939
                     /standard_name="lac operator"
     promoter        complement(5947..5977)
     protein_bind    5992..6013
                     /standard_name="CAP binding site"
     misc_feature    6120..7262
                     /standard_name="BlastR cassette"
     polyA_signal    6130..6251
                     /note="Geneious type: polya_signal"
     misc_feature    complement(6177..6251)
                     /standard_name="SV40 late terminator"
     gene            complement(6409..6807)
                     /standard_name="BlastR Gene"
     misc_feature    complement(6808..6873)
                     /standard_name="EM7 promoter"
     promoter        complement(6921..7250)
     rep_origin      6935..7070
     misc_feature    complement(6946..7021)
                     /standard_name="SV40 ORI"
     misc_feature    complement(6978..7259)
                     /standard_name="SV40 early promoter"
     misc_feature    complement(7096..7121)
                     /note="Geneious type: user"
                     /standard_name="350_010818_BlastR_FwU"
     primer_bind     complement(7098..7122)
                     /standard_name="ACK18"
     misc_feature    7358..7397
                     /note="Geneious type: user"
                     /standard_name="071718_Tol5'_PciI_InF_Fw"
     LTR             7374..7573
     misc_feature    complement(7553..7588)
                     /note="Geneious type: user"
                     /standard_name="071718_Tol5'_InF_Rv"
     rep_origin      7616..8296
     misc_feature    8256
                     /standard_name="G>A"
     misc_feature    8286..8296
                     /standard_name="USER jct 25"
     misc_feature    complement(8393..9253)
                     /note="Geneious type: Marker"
                     /standard_name="bla (AmpR)"
     misc_feature    8533..8538
                     /standard_name="removed BsaI site"
     misc_feature    8536..8544
                     /standard_name="USER jct 31"
     promoter        complement(9295..9323)
                     /note="Geneious type: Promoter"
     misc_feature    9611..9643
                     /note="Geneious type: user"
                     /standard_name="071718_Tol3'_InF_Fw"
     LTR             9626..9798
     misc_feature    complement(9769..9814)
                     /note="Geneious type: user"
                     /standard_name="071718_Tol3'_MluI_InF_Rv"
     misc_feature    10011
                     /standard_name="C"
ORIGIN      
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca atgaaacgga cagccgacgg aagcgagttc gagtcaccaa
      121 agaagaagcg gaaagtcgag gcaagcccag caagcggccc acggcacctg atggaccccc
      181 acattttcac cagcaacttc aacaacggaa tcggacgaca caagacctac ctgtgctatg
      241 aggtggagag gctggacaac ggcacatccg tgaagatgga tcagcacaga ggcttcctgc
      301 acggccaggc caagaatctg ctgtgcggct tctacggccg gcacgcagag ctgcgctttc
      361 tggacctggt gccatctctg cagctggatc ccgcccagat ctatagggtg acctggttca
      421 tcagctggtc cccttgcttt agctggggat gtgcaggaga ggtgagggcc ttcctgcagg
      481 agaacacaca cgtgaggctg agaatctttg ccgcccggat ctacgactat gatcccctgt
      541 acaaggaggc cctgcagatg ctgagggacg caggagccca ggtgtctatc atgacctatg
      601 atgagttcaa gcactgctgg gacacatttg tggatcacca gggctgtccc tttcagcctt
      661 gggacggcct ggacgagcat tcccaggcac tgagcggacg gctgagggca attctgcaga
      721 accagggcaa ttctggcgga tctagcggag gatcctctgg cagcgagaca ccaggaacaa
      781 gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcgac aagaagtaca
      841 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      901 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      961 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1021 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1081 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1141 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1201 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1261 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1321 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1381 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1441 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1501 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1561 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1621 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1681 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1741 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1801 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1861 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1921 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1981 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     2041 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2101 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2161 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2221 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2281 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2341 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2401 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2461 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2521 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2581 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2641 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2701 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2761 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2821 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2881 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2941 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3001 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3061 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3121 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3181 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3241 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3301 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     3361 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3421 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3481 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3541 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3601 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3661 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3721 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3781 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3841 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3901 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3961 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4021 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4081 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4141 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4201 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4261 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4321 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4381 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4441 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4501 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4561 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4621 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4681 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4741 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4801 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4861 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4921 gaggtgacag cggcgggagc ggcgggagcg gggggagcac taatctgagc gacatcattg
     4981 agaaggagac tgggaaacag ctggtcattc aggagtccat cctgatgctg cctgaggagg
     5041 tggaggaagt gatcggcaac aagccagagt ctgacatcct ggtgcacacc gcctacgacg
     5101 agtccacaga tgagaatgtg atgctgctga cctctgacgc ccccgagtat aagccttggg
     5161 ccctggtcat ccaggattct aacggcgaga ataagatcaa gatgctgagc ggaggatccg
     5221 gaggatctgg aggcagcacc aacctgtctg acatcatcga gaaggagaca ggcaagcagc
     5281 tggtcatcca ggagagcatc ctgatgctgc ccgaagaagt cgaagaagtg atcggaaaca
     5341 agcctgagag cgatatcctg gtccataccg cctacgacga gagtaccgac gaaaatgtga
     5401 tgctgctgac atccgacgcc ccagagtata agccctgggc tctggtcatc caggattcca
     5461 acggagagaa caaaatcaaa atgctgtctg gcggctcaaa aagaaccgcc gacggcagcg
     5521 aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc accattgagt
     5581 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5641 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5701 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5761 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5821 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     5881 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     6001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcta
     6121 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6181 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6241 ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     6301 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     6361 tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgctt agccctccca
     6421 cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc
     6481 cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca
     6541 ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc
     6601 tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg
     6661 atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct
     6721 gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga
     6781 ttcttcttga gacaaaggct tggccatggt ttagttcctc accttgtcgt attatactat
     6841 gccgatatac tatgccgatg attaattgtc aacacgtgct gatcagatcc gaaaatggat
     6901 atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     6961 ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt
     7021 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     7081 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     7141 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     7201 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacaga
     7261 attcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtaagtgat
     7381 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     7441 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     7501 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     7561 ctttacacct ctgcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8221 aagaagatcc tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt
     8281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8521 caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9361 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     9421 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9481 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9541 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9601 gcgatgtacg ggccagatat acgcgggtac cggcatatgg ttcttgacag aggtgtaaaa
     9661 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
     9721 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
     9781 aattgtactt gagtattaac gcgttgacat tgattattga ctagttatta atagtaatca
     9841 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9901 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9961 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10021 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10081 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10141 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
    10201 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
    10261 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
    10321 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
    10381 agc
//
