LOCUS       pLX304_CMV_FKBP-V5-sTur 8310 bp ds-DNA     circular     01-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2030..2210
                     /label="HIV1 3' LTR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    634..636
                     /label="kozak"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    622..631
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1285..1290
                     /label="NheI"
                     /ApEinfo_revcolor=#ff0d89
                     /ApEinfo_fwdcolor=#ff0d89
     misc_feature    5643..5668
                     /label="Fwd sequencing primer_6720"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    523..543
                     /label="CMVf"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     primer_bind     5420..5439
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     5382..5402
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5692..5872
                     /label="HIV1 5'LTR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     CDS             2721..2789
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1279..1284
                     /label="double stop"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    595..602
                     /label="attR1+teminus"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5983..6027
                     /label="Psi Packaging Signal"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    1027..1056
                     /label="Linker"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    6537..6770
                     /label="RRE"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4332..5014
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(2604..2624)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3575..4234
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    640..954
                     /label="FKBP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1982..2006
                     /label="Sequencing primer_fwd_3059"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    985..1026
                     /label="V5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    603..619
                     /label="attB1"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     misc_feature    523..543
                     /label="CMVf"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    637..639
                     /label="Start"
                     /ApEinfo_revcolor=#ff3adb
                     /ApEinfo_fwdcolor=#ff3adb
     misc_feature    14..566
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    6794..6816
                     /label="Sequencing primer_Rev_7893"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    955..984
                     /label="Linker"
                     /ApEinfo_revcolor=#a478ff
                     /ApEinfo_fwdcolor=#a478ff
     misc_binding    5354..5376
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      2808..3114
                     /label="F1 ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1057..1062)
                     /label="AgeI site (TG)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    595..602
                     /label="attR1"
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_fwdcolor=#004080
     misc_feature    1063..1278
                     /label="sN Turbo L73"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(2633..2650)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1252..1254
                     /label="Q65P"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttGTACAAAA AAGCAGGCTT CGAAGGAGAT AGAACCATGC AGGTGGAAAC CATCTCCCCA
      661 GGAGACGGGC GCACCTTCCC CAAGCGCGGC CAGACCTGCG TGGTGCACTA CACCGGGATG
      721 CTTGAAGATG GAAAGAAATT TGATTCCTCC CGGGACAGAA ACAAGCCCTT TAAGTTTATG
      781 CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG GAAGAAGGGG TTGCCCAGAT GAGTGTGGGT
      841 CAGAGAGCCA AACTGACTAT ATCTCCAGAT TATGCCTATG GTGCCACTGG GCACCCAGGC
      901 ATCATCCCAC CACATGCCAC TCTCGTCTTC GATGTGGAGC TTCTAAAACT GGAAGGGAGT
      961 GGCAGCGGAG GTAGTGGGAG CGGTGGCAAG CCCATCCCCA ACCCCCTGCT GGGCCTGGAC
     1021 AGCACCAAGG GCTCGGGCTC GACCTCGGGC TCGGGCACCG GTAAAGACAA TACTGTGCCT
     1081 CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA GTGGCGAACA GCTGGGAGAA
     1141 ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC AGACTCTGCG CGACTGGGGC
     1201 GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC CCGAGCCTAT CCCGCTGCTG
     1261 AACGCTAAAC AGATTCTGTA ATAAgctagc gctaaccggt ggcgcgttaa gtcgacaatc
     1321 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1381 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1441 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1501 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1561 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     1621 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1681 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     1741 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     1801 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     1861 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     1921 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1981 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2041 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2101 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2161 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     2221 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     2281 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2401 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     2461 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2521 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2581 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     2641 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2701 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2761 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     2821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3241 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3301 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3361 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3421 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3481 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3541 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3601 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3661 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3721 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3781 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3841 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3901 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3961 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4021 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4081 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4141 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4201 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5161 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5221 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5281 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5341 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5401 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     5461 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     5521 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     5581 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     5641 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     5701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     5881 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     5941 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6001 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6061 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6121 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6181 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6241 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6301 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6361 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     6421 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6481 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     6541 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     6601 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     6661 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     6721 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     6781 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     6841 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     6901 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     6961 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     7021 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7081 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7141 tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg
     7201 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     7261 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     7321 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     7381 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     7441 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     7501 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     7561 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     7621 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
     7681 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     7741 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     7801 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     7861 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     7921 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     7981 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     8041 ggattcgtga attgctgccc tctggttatg tgtgggaggg cctgcagctg cagtagtaag
     8101 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     8161 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     8221 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     8281 gagatccact ttggcgccgg ctcgaggggg
//