LOCUS       pLX304_CMV_FKBP-V5-sTur 8325 bp ds-DNA     circular     01-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    634..636
                     /label="kozak"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    595..602
                     /label="attR1+teminus"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      2823..3129
                     /label="F1 ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1300..1305
                     /label="NheI"
                     /ApEinfo_revcolor=#ff0d89
                     /ApEinfo_fwdcolor=#ff0d89
     misc_feature    523..543
                     /label="CMVf"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    1027..1056
                     /label="Linker"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    640..954
                     /label="FKBP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    595..602
                     /label="attR1"
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_fwdcolor=#004080
     misc_feature    985..1026
                     /label="V5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1997..2021
                     /label="Sequencing primer_fwd_3059"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    603..619
                     /label="attB1"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     misc_binding    5369..5391
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    523..543
                     /label="CMVf"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     5397..5417
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      4347..5029
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1252..1254
                     /label="Q65P"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5658..5683
                     /label="Fwd sequencing primer_6720"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     5435..5454
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2736..2804
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6552..6785
                     /label="RRE"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1294..1299
                     /label="double stop"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    5707..5887
                     /label="HIV1 5'LTR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1063..1293
                     /label="sTurboID G78"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5998..6042
                     /label="Psi Packaging Signal"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    955..984
                     /label="Linker"
                     /ApEinfo_revcolor=#a478ff
                     /ApEinfo_fwdcolor=#a478ff
     misc_feature    637..639
                     /label="Start"
                     /ApEinfo_revcolor=#ff3adb
                     /ApEinfo_fwdcolor=#ff3adb
     primer_bind     complement(2648..2665)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3590..4249
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    622..631
                     /label="Shine-Dalgarno"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1057..1062)
                     /label="AgeI site (TG)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2619..2639)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2045..2225
                     /label="HIV1 3' LTR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    14..566
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    6809..6831
                     /label="Sequencing primer_Rev_7893"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttGTACAAAA AAGCAGGCTT CGAAGGAGAT AGAACCATGC AGGTGGAAAC CATCTCCCCA
      661 GGAGACGGGC GCACCTTCCC CAAGCGCGGC CAGACCTGCG TGGTGCACTA CACCGGGATG
      721 CTTGAAGATG GAAAGAAATT TGATTCCTCC CGGGACAGAA ACAAGCCCTT TAAGTTTATG
      781 CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG GAAGAAGGGG TTGCCCAGAT GAGTGTGGGT
      841 CAGAGAGCCA AACTGACTAT ATCTCCAGAT TATGCCTATG GTGCCACTGG GCACCCAGGC
      901 ATCATCCCAC CACATGCCAC TCTCGTCTTC GATGTGGAGC TTCTAAAACT GGAAGGGAGT
      961 GGCAGCGGAG GTAGTGGGAG CGGTGGCAAG CCCATCCCCA ACCCCCTGCT GGGCCTGGAC
     1021 AGCACCAAGG GCTCGGGCTC GACCTCGGGC TCGGGCACCG GTAAAGACAA TACTGTGCCT
     1081 CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA GTGGCGAACA GCTGGGAGAA
     1141 ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC AGACTCTGCG CGACTGGGGC
     1201 GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC CCGAGCCTAT CCCGCTGCTG
     1261 AACGCTAAAC AGATTCTGGG ACAGCTGGAC GGCTAATAAg ctagcgctaa ccggtggcgc
     1321 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1381 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1441 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     1501 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     1561 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     1621 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     1681 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     1741 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     1801 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     1861 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     1921 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1981 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2041 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2221 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     2281 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     2341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2401 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     2461 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2521 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2581 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     2641 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2701 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2761 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     2821 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2881 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2941 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3001 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3061 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3121 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3181 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3241 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3841 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3901 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3961 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4021 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4081 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4141 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4201 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5101 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5161 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5221 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5281 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5341 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5401 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     5461 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     5521 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     5581 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     5641 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     5701 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5761 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5821 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5881 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5941 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6001 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6061 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6121 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6181 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     6241 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     6301 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     6361 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     6421 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     6481 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     6541 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     6601 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     6661 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     6721 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     6781 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     6841 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6901 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6961 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7021 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7081 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7141 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     7201 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     7261 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     7321 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     7381 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     7441 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     7501 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     7561 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     7621 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     7681 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     7741 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7801 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7861 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     7921 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     7981 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8041 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     8101 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     8161 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8221 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8281 attacaggga cagcagagat ccactttggc gccggctcga ggggg
//