LOCUS       pCAG_CAG_Glucagon-ICAM1 8344 bp ds-DNA     linear       01-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3420..3421
                     /label="CD4"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3294..3299
                     /label="AgeI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3414..3419
                     /label="AflII"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    1825..1825
                     /label="arg17->lys"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4080..4244
                     /label="NXN cytoplasmic region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3288..3293
                     /label="HpaI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3408..3413
                     /label="AgeI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1929..3287
                     /label="ICAM1-EC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5031..5222
                     /label="BGH polyA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3300..3407
                     /label="3xHA"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    3422..4079
                     /label="CD4"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3420..3421
                     /label="pre mgrasp"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1838..1838
                     /label="asp21->glu"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(5869..5891)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1731..1775
                     /label="Trypsin Signal Peptide"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4245..4250
                     /label="AflII"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    1827..1829
                     /label="arg18->lys"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(5843..5863)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1776..1862
                     /label="hGlucagon"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4251..4253
                     /label="STOP CODON"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1..360
                     /label="Cmv enhancer"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1869..1922
                     /label="flexible linker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(6576..7258)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6222..6350
                     /label="SV40 early polyA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1..1622
                     /label="CAGGS promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3422..4244
                     /label="pre mgrasp"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1725..1730
                     /label="KpnI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(7356..8015)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcggtacc ATGAGTGCAC
     1741 TTCTGATCCT AGCCCTTGTG GGAGCTGCTG TTGCTCATTC ACAGGGCACA TTCACCAGTG
     1801 ACTACAGCAA GTATCTGGAC TCCAAGAAGG CCCAAGAGTT TGTGCAGTGG TTGATGAATA
     1861 CAGGTACCGG GAACGGAAAT GGCAATGGTA ACGGGAATGG AAACGGCAAT GGTAACGGGA
     1921 ATACTAGTCA GACATCTGTG TCCCCCTCAA AAGTCATCCT GCCCCGGGGA GGCTCCGTGC
     1981 TGGTGACATG CAGCACCTCC TGTGACCAGC CCAAGTTGTT GGGCATAGAG ACCCCGTTGC
     2041 CTAAAAAGGA GTTGCTCCTG CCTGGGAACA ACCGGAAGGT GTATGAACTG AGCAATGTGC
     2101 AAGAAGATAG CCAACCAATG TGCTATTCAA ACTGCCCTGA TGGGCAGTCA ACAGCTAAAA
     2161 CCTTCCTCAC CGTGTACTGG ACTCCAGAAC GGGTGGAACT GGCACCCCTC CCCTCTTGGC
     2221 AGCCAGTGGG CAAGAACCTT ACCCTACGCT GCCAGGTGGA GGGTGGGGCA CCCCGGGCCA
     2281 ACCTCACCGT GGTGCTGCTC CGTGGGGAGA AGGAGCTGAA ACGGGAGCCA GCTGTGGGGG
     2341 AGCCCGCTGA GGTCACGACC ACGGTGCTGG TGAGGAGAGA TCACCATGGA GCCAATTTCT
     2401 CGTGCCGCAC TGAACTGGAC CTGCGGCCCC AAGGGCTGGA GCTGTTTGAG AACACCTCGG
     2461 CCCCCTACCA GCTCCAGACC TTTGTCCTGC CAGCGACTCC CCCACAACTT GTCAGCCCCC
     2521 GGGTCCTAGA GGTGGACACG CAGGGGACCG TGGTCTGTTC CCTGGACGGG CTGTTCCCAG
     2581 TCTCGGAGGC CCAGGTCCAC CTGGCACTGG GGGACCAGAG GTTGAACCCC ACAGTCACCT
     2641 ATGGCAACGA CTCCTTCTCG GCCAAGGCCT CAGTCAGTGT GACCGCAGAG GACGAGGGCA
     2701 CCCAGCGGCT GACGTGTGCA GTAATACTGG GGAACCAGAG CCAGGAGACA CTGCAGACAG
     2761 TGACCATCTA CAGCTTTCCG GCGCCCAACG TGATTCTGAC GAAGCCAGAG GTCTCAGAAG
     2821 GGACCGAGGT GACAGTGAAG TGTGAGGCCC ACCCTAGAGC CAAGGTGACG CTGAATGGGG
     2881 TTCCAGCCCA GCCACTGGGC CCGAGGGCCC AGCTCCTGCT GAAGGCCACC CCAGAGGACA
     2941 ACGGGCGCAG CTTCTCCTGC TCTGCAACCC TGGAGGTGGC CGGCCAGCTT ATACACAAGA
     3001 ACCAGACCCG GGAGCTTCGT GTCCTGTATG GCCCCCGACT GGACGAGAGG GATTGTCCGG
     3061 GAAACTGGAC GTGGCCAGAA AATTCCCAGC AGACTCCAAT GTGCCAGGCT TGGGGGAACC
     3121 CATTGCCCGA GCTCAAGTGT CTAAAGGATG GCACTTTCCC ACTGCCCATC GGGGAATCAG
     3181 TGACTGTCAC TCGAGATCTT GAGGGCACCT ACCTCTGTCG GGCCAGGAGC ACTCAAGGGG
     3241 AGGTCACCCG CGAGGTGACC GTGAATGTGC TCTCCCCCCG GTATGAGGTT AACACCGGTG
     3301 CGGCCGTTTA CCCATACGAT GTTCCTGACT ATGCGGGCTA TCCCTATGAC GTCCCGGACT
     3361 ATGCAGGATC CTATCCATAT GACGTTCCAG ATTACGCTCC GGCCGCCACC GGTCTTAAGG
     3421 TCGACTTTCA GAAAGCTAGC TCTATTGTTT ATAAGAAGGA GGGGGAACAG GTGGAGTTCA
     3481 GTTTCCCATT GGCCTTCACA GTCGAGAAGC TCACAGGATC AGGAGAGTTG TGGTGGCAGG
     3541 CCGAAAGAGC CTCCTCCAGC AAGAGTTGGA TCACTTTTGA TCTGAAAAAC AAAGAAGTGA
     3601 GCGTGAAGAG AGTCACACAG GACCCTAAGC TGCAGATGGG CAAGAAGTTG CCTTTGCACT
     3661 TGACACTCCC CCAAGCCCTG CCCCAGTATG CTGGCTCCGG GAACCTCACC CTCGCCTTGG
     3721 AGGCTAAGAC CGGAAAGTTG CATCAGGAGG TCAACCTCGT GGTGATGAGG GCAACACAGC
     3781 TGCAGAAGAA CCTGACCTGC GAAGTGTGGG GTCCCACCTC ACCTAAACTG ATGCTGTCTC
     3841 TGAAGCTGGA GAATAAGGAG GCTAAAGTCA GTAAAAGAGA GAAGGCTGTG TGGGTCCTTA
     3901 ACCCGGAGGC AGGGATGTGG CAGTGTCTTC TTAGCGACTC TGGACAGGTG CTGCTGGAGA
     3961 GCAACATCAA GGTGCTTCCT ACTTGGTCAA CACCAGTACA GCCGATGGCC CTGATCGTAC
     4021 TCGGGGGAGT CGCCGGCCTG CTTCTGTTTA TCGGCCTGGG CATCTTCTTC TGCGTACGCT
     4081 ACAAGTATCG CAACAGGGAT GAGGGATCTT ACCACGTTGA CGAATCTAGG AACTATATCA
     4141 GTAACAGCGC TCAGAGTAAT GGCGCCGTGG TTAAAGAGAA ACAGCCCTCC AGCGCCAAAT
     4201 CAGCAAACAA AAACAAGAAA AACAAGGATA AAGAGTACTA CGTACTTAAG taactcgagg
     4261 ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag accacataga aactgggctt
     4321 gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca
     4381 ctttgccttt ctctccacag gtgtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     4441 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4501 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4561 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4621 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4681 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4741 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4801 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4861 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4921 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4981 ttgggccgcc tccccgcctg gaattcgagc tcggtacgat cagcctcgac tgtgccttct
     5041 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5101 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5161 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5221 agcaggccag cttttgttcc ctttagggtt aattgcgcgc ttggcgctaa tcatggtcat
     5281 agctgtttcc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5341 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5401 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5461 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5521 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     5581 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     5641 ccttattcca tagaaaagcc ttgacttgag gttagatttt tttttatatt ttgtttgtgt
     5701 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     5761 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc
     5821 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5881 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5941 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6001 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     6061 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6121 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6181 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     6241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     6361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6781 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7021 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7201 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7261 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7321 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7381 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7441 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7501 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7561 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7621 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7681 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7741 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7801 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7861 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7921 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7981 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8041 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8101 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8161 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8221 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8281 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8341 cctg
//