LOCUS       _pAAV-Syn-Synapt        6650 bp    DNA     linear       04-MAY-2020
FEATURES             Location/Qualifiers
     LTR             44..185
                     /note="UTR"
                     /dnas_title="UTR%1E%1F"
     promoter        195..660
                     /note="Synapsin Promoter"
     source          join(45..6650,1..44)
                     /dnas_title="_pAAV-Syn-SynaptoPAC-minWPRE"
     gene            673..1611
                     /note="Synaptophysin"
     misc_feature    1616..1635
                     /note="linker"
     gene            1636..2331
                     /note="mScarlet"
     gene            2338..3387
                     /note="bPAC"
     iDNA            3498..3935
                     /note="min WPRE"
     misc_feature    3439..3456
                     /note="His Tag"
     misc_feature    3400..3423
                     /note="myc tag"
     LTR             3957..4097
                     /note="3%B4UTR"
     rep_origin      4189..4495
                     /label=f1_origin
     promoter        4944..4972
                     /label=AmpR_promoter
     CDS             5014..5874
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      6029..6648
                     /label=pBR322_origin
ORIGIN      
        1 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttcctgcggc cgcaCGCGTA GAGGGCCCTG CGTATGAGTG CAAGTGGGTT TTAGGACCAG
      241 GATGAGGCGG GGTGGGGGTG CCTACCTGAC GACCGACCCC GACCCACTGG ACAAGCACCC
      301 AACCCCCATT CCCCAAATTG CGCATCCCCT ATCAGAGAGG GGGAGGGGAA ACAGGATGCG
      361 GCGAGGCGCG TGCGCACTGC CAGCTTCAGC ACCGCGGACA GTGCCTTCGC CCCCGCCTGG
      421 CGGCGCGCGC CACCGCCGCC TCAGCACTGA AGGCGCGCTG ACGTCACTCG CCGGTCCCCC
      481 GCAAACTCCC CTTCCCGGCC ACCTTGGTCG CGTCCGCGCC GCCGCCGGCC CAGCCGGACC
      541 GCACCACGCG AGGCGCGAGA TAGGGGGGCA CGGGCGCGAC CATCTGCGCT GCGGCGCCGG
      601 CGACTCAGCG CTGCCTCAGT CTGCGGTGGG CAGCGGAGGA GTCGTGTCGT GCCTGAGAGC
      661 GCAGTCGcta gcatgctgct gctggcagac atggacgtgg tgaatcagct ggtggctggg
      721 ggtcagttcc gggtggtcaa ggagcccctt ggcttcgtga aggtgctgca gtgggtcttt
      781 gccatcttcg cctttgctac gtgcggcagc tacaccggag agcttcggct gagcgtggag
      841 tgtgccaaca agacggagag tgccctcaac atcgaagtcg aatttgagta cccattcagg
      901 ctgcaccaag tgtactttga tgcaccctcc tgcgttaaag ggggcactac caagatcttc
      961 ctagttggtg actactcctc ctcggctgaa ttctttgtca ccgtggctgt gtttgccttc
     1021 ctctactcca tgggggccct ggccacctac atcttcctgc agaacaagta ccgagagaac
     1081 aacaaagggc caatgatgga cttcctggcc acagcagtgt tcgctttcat gtggctagtt
     1141 agctcatccg cctgggccaa aggcctgtcc gatgtgaaga tggccactga cccagagaac
     1201 attatcaagg agatgcctat gtgccgccag acaggaaaca catgcaagga actgagggac
     1261 cctgtgactt caggactcaa cacctcggtg gtgtttggct tcctgaacct ggtgctctgg
     1321 gttggcaacc tatggttcgt gttcaaggag acaggctggg ccgccccatt catgcgcgca
     1381 cctccaggcg ccccagaaaa gcaaccagct cctggcgatg cctacggcga tgcgggctat
     1441 gggcagggcc ccggaggcta tgggccccag gactcctacg ggcctcaggg tggttatcaa
     1501 cccgattacg ggcagccagc cagcggcggt ggcggtggct acgggcctca gggcgactat
     1561 gggcagcaag gctacggcca acagggtgcg cccacctcct tctccaatca gatggaaggg
     1621 ttaactggct ccaccatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     1681 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     1741 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1801 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcaccaag
     1861 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     1921 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     1981 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     2041 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     2101 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg
     2161 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     2221 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     2281 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa ggGATCCATG
     2341 ATGAAGCggc tggtgtacat cagcaagatc agcggccacc tgagcctgga agagatccag
     2401 cggatcggca aggtgtccat caagaacaac cagcgggaca acatcaccgg cgtgctgctg
     2461 tacctgcagg gcctgttctt ccagatcctg gaaggcgaga acgagaaggt ggacaagctg
     2521 tacaagaaaa tcctggtgga cgaccggcac accaacatcc tgtgcctgaa aaccgagtac
     2581 gacatcaccg accggatgtt ccccaactgg gccatgaaaa ccatcaacct gaacgagaac
     2641 agcgagctga tgatccagcc catcaagagc ctgctgcaga ccatcaccca gagccaccgg
     2701 gtgctggaaa agtacatgcc cgccagagtg atctacctga tcaaccaggg catcaacccc
     2761 ctgaccgtgg agccccagct ggtcgagaag atcatcttct tcagcgacat cctggccttc
     2821 agcaccctga ccgagaagct gcccgtgaac gaggtggtca tcctggtcaa ccggtacttc
     2881 agcatctgca cccggatcat cagcgcctac ggcggcgaag tgaccaagtt catcggcgac
     2941 tgcgtgatgg ccagcttcac caaagagcag ggcgacgccg ccatccggac cagcctggac
     3001 atcatctccg agctgaagca gctgcggcac cacgtggagg ccaccaaccc cctgcacctg
     3061 ctgtacaccg gcatcggcct gagctacggc cacgtgatcg agggcaacat gggcagcagc
     3121 ctgaagatgg accacaccct gctgggggac gccgtgaacg tggccgccag gctggaagcc
     3181 ctgacaagac agctgcccta cgccctggcc tttacagccg gcgtgaagaa gtgctgccag
     3241 gcccagtgga ccttcatcaa cctgggagcc caccaggtca agggcaagca ggaagccatc
     3301 gaggtctaca ccgtgaatga ggcccagaag tactacgaca ccctgcagat cacccagctg
     3361 atccggcaga ccctggaaaa cgacaagccg cggacgtacg agcaaaagct gatctcggag
     3421 gaggacctga agggatcgca ccaccatcac caccatgcat aaccttcgaa ttaatTAAGG
     3481 CCGCGACTCT agaattcCGC tcgagataat caacctctgg attacaaaat ttgtgaaaga
     3541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3661 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3721 gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt
     3781 gctttatttg taaccattct agctttattt gtgaaatttg tgatgctatt gctttatttg
     3841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3901 aggttcaggg ggagatgtgg gaggtttttt aaagcacgtg cggaccgagc ggccgcagga
     3961 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4021 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4081 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4141 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4381 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4501 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4621 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4681 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4741 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4801 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
//

