LOCUS       Exported                3175 bp ds-DNA     linear   UNA 05-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..131
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    186..306
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     primer_bind     186..205
                     /label=PCF
                     /note="color: black; sequence: CCATTGACGTCAATAATGAC"
     misc_feature    307..1331
                     /label=mSncg 1.03kb Promoter
                     /note="color: #a6acb3"
     misc_feature    1338..2057
                     /label=EGFP
                     /note="color: #a6acb3"
     primer_bind     complement(1371..1388)
                     /label=PGR
                     /note="color: black; sequence: GACCAGGATGGGCACCAC"
     misc_feature    2109..2714
                     /label=WPRE
                     /note="WPRE color: #00ff80"
                     /note="color: #a6acb3"
     primer_bind     complement(2363..2382)
                     /label=PWR
                     /note="color: black; sequence: GCTGACAGGTGGTGGCAATG"
     misc_feature    2715..2894
                     /label=BGH polyA
                     /note="BGH polyA color: #00ffff"
                     /note="color: #a6acb3"
     misc_feature    complement(3046..3175)
                     /label=ITR(1)
                     /note="ITR color: #ff8080"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatcag ccttacaagt gactctcaaa tttgctggtg atgtggtctt caagcgaaat
      361 gtcagaaaga aaagaaaaca cgaggacaac aaagggagga agtggcctgg tccggcccac
      421 ccggcaagtc tcatccgccc ccgcccccgc cccttccagc ctggccccct tggaggcctc
      481 caaccactca ggtcaattcc tgtgtcctga gggcacttga atcagggaca cgggatttgg
      541 tagacacata aaggtggccc cattaaactt atttctccag gactctgtcg tgggcctgga
      601 ggagatctgg tgccacccat actgttggcc aggaagtggg gaacgggcac atcacacctg
      661 ctcggcacct tgggctatgg gaactagcag gtgggtggga actcagagaa ggaaagggac
      721 tatgctagaa tcacacagcg ggcagcccag tctagggcat ggggagcagc tttgggtgtt
      781 tctggcctca gccttccaac aggtttggct agagctccag gctcaagagc atccaggata
      841 cagtggggaa ctggataaca gggcagcctg caggttggcc attcattgga ttggccctga
      901 ccccggccca gcctgggaca ctgaggcatc atcagtcaag gcacttttct tctgcatata
      961 agagccaggg cacgagacca ccagggcttt ccaaggatga atgaggtgta atgatagatt
     1021 aggatatgtc cagcctccaa cacgctctcc ctcccccagg gccaacaaga gtcagcaggg
     1081 cagaatagag ccagtagggg cccgggccct gctcgctggt atccccgtga ggcatgcctt
     1141 ctctctggcc cgccctccct gcccccaccc tggcccgggc tggctgggct ccagccagca
     1201 gccacagcat caatatttca tctgcgtcaa taagaggcag tagcagcaga gacagcggct
     1261 gcggcagcac tccagtccat agcttgcagc agccaggttc catccttgca aacaccatgg
     1321 acgtcttcaa ggaattcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1381 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1441 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1501 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1561 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1621 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1681 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1741 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1801 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1861 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1921 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1981 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2041 acgagctgta caagtaaaga tctacgcgtg gtacctctag agtcgacccg ggcggcctcg
     2101 agaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2701 cgcatcgata ccgtcgactc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2941 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc
     3001 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
     3061 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3121 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcag
//
