LOCUS       Exported                2996 bp ds-DNA     linear   UNA 05-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
     misc_feature    1..131
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    186..430
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     primer_bind     186..205
                     /label=PCF
                     /note="color: black; sequence: CCATTGACGTCAATAATGAC"
     misc_feature    448..1121
                     /label=CAG promoter
                     /note="color: #a6acb3"
     misc_feature    1159..1878
                     /label=EGFP
                     /note="color: #a6acb3"
     primer_bind     complement(1192..1209)
                     /label=PGR
                     /note="color: black; sequence: GACCAGGATGGGCACCAC"
     misc_feature    1930..2535
                     /label=WPRE
                     /note="WPRE color: #00ff80"
                     /note="color: #a6acb3"
     primer_bind     complement(2184..2203)
                     /label=PWR
                     /note="color: black; sequence: GCTGACAGGTGGTGGCAATG"
     misc_feature    2536..2715
                     /label=BGH polyA
                     /note="BGH polyA color: #00ffff"
                     /note="color: #a6acb3"
     misc_feature    complement(2867..2996)
                     /label=ITR(1)
                     /note="ITR color: #ff8080"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      361 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      421 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      481 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      541 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
      601 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      661 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      721 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      781 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      841 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      901 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggctgtcc gcggggggac
      961 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1021 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1081 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tggatccacc ggtgcggccg
     1141 cacgcgtctc gagaattcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1201 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1261 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1321 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1381 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1441 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1501 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1561 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1621 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1681 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1741 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1801 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1861 gacgagctgt acaagtaaag atctacgcgt ggtacctcta gagtcgaccc gggcggcctc
     1921 gagaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2041 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2101 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2161 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2221 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2281 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2341 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2401 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2461 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2521 ccgcatcgat accgtcgact cgctgatcag cctcgactgt gccttctagt tgccagccat
     2581 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2641 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2701 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2761 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgactagag
     2821 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     2881 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     2941 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcag
//
