LOCUS       Exported                3442 bp ds-DNA     linear   UNA 06-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
     misc_feature    1..131
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    186..308
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     primer_bind     186..205
                     /label=PCF
                     /note="color: black; sequence: CCATTGACGTCAATAATGAC"
     misc_feature    309..1598
                     /label=mCamK2a
                     /note="color: #a6acb3"
     misc_feature    1605..2324
                     /label=EGFP
                     /note="color: #a6acb3"
     primer_bind     complement(1638..1655)
                     /label=PGR
                     /note="color: black; sequence: GACCAGGATGGGCACCAC"
     misc_feature    2376..2981
                     /label=WPRE
                     /note="WPRE color: #00ff80"
                     /note="color: #a6acb3"
     primer_bind     complement(2630..2649)
                     /label=PWR
                     /note="color: black; sequence: GCTGACAGGTGGTGGCAATG"
     misc_feature    2982..3161
                     /label=BGH polyA
                     /note="BGH polyA color: #00ffff"
                     /note="color: #a6acb3"
     misc_feature    complement(3313..3442)
                     /label=ITR(1)
                     /note="ITR color: #ff8080"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc attatggcct taggtcactt catctccatg gggttcttct tctgattttc
      361 tagaaaatga gatgggggtg cagagagctt cctcagtgac ctgcccaggg tcacatcaga
      421 aatgtcagag ctagaacttg aactcagatt actaatctta aattccatgc cttgggggca
      481 tgcaagtacg atatacagaa ggagtgaact cattagggca gatgaccaat gagtttagga
      541 aagaagagtc cagggcaggg tacatctaca ccacccgccc agccctgggt gagtccagcc
      601 acgttcacct cattatagtt gcctctctcc agtcctacct tgacgggaag cacaagcaga
      661 aactgggaca ggagccccag gagaccaaat cttcatggtc cctctgggag gatgggtggg
      721 gagagctgtg gcagaggcct caggaggggc cctgctgctc agtggtgaca gataggggtg
      781 agaaagcaga cagagtcatt ccgtcagcat tctgggtctg tttggtactt cttctcacga
      841 taaggtggcg gtgtgatatg cacaatggct aaaaagcagg gagagctgga aagaaacaag
      901 gacagagaca gaggccaagt caaccagacc aattcccaga ggaagcaaag aaaccattac
      961 agagactaca agggggaagg gaaggagaga tgaattagct tcccctgtaa accttagaac
     1021 ccagctgttg ccagggcaac ggggcaatac ctgtctcttc agaggagatg aagttgccag
     1081 ggtaactaca tcctgtcttt ctcaaggacc atcccagaat gtggcaccca ctagccgtta
     1141 ccatagcaac tgcctctttg ccccacttaa tcccatcccg tctgttaaaa gggccctata
     1201 gttggaggtg ggggaggtag gaagagcgat gatcacttgt ggactaagtt tgttcgcatc
     1261 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
     1321 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
     1381 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
     1441 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
     1501 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
     1561 gcacgggcag gcgagtggcc cctagttctg ggggcagcga attcatggtg agcaagggcg
     1621 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1681 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1741 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1801 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1861 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1921 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1981 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2041 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2101 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2161 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2221 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2281 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagatct acgcgtggta
     2341 cctctagagt cgacccgggc ggcctcgaga agcttatcga taatcaacct ctggattaca
     2401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     2821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2941 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgactcgct gatcagcctc
     3001 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3061 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3121 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3181 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     3241 aagaaccagc tggggctcga ctagagcatg gctacgtaga taagtagcat ggcgggttaa
     3301 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3361 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3421 cagtgagcga gcgagcgcgc ag
//
