LOCUS       Exported                3501 bp ds-DNA     linear   UNA 06-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
     misc_feature    1..131
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    186..308
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     primer_bind     186..205
                     /label=PCF
                     /note="color: black; sequence: CCATTGACGTCAATAATGAC"
     misc_feature    309..1657
                     /label=hSncg Promoter
                     /note="color: #a6acb3"
     misc_feature    1664..2383
                     /label=EGFP
                     /note="color: #a6acb3"
     primer_bind     complement(1697..1714)
                     /label=PGR
                     /note="color: black; sequence: GACCAGGATGGGCACCAC"
     misc_feature    2435..3040
                     /label=WPRE
                     /note="WPRE color: #00ff80"
                     /note="color: #a6acb3"
     primer_bind     complement(2689..2708)
                     /label=PWR
                     /note="color: black; sequence: GCTGACAGGTGGTGGCAATG"
     misc_feature    3041..3220
                     /label=BGH polyA
                     /note="BGH polyA color: #00ffff"
                     /note="color: #a6acb3"
     misc_feature    complement(3372..3501)
                     /label=ITR(1)
                     /note="ITR color: #ff8080"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aaggtccagg ccctcttacc gtcctacggg gtccttgccg gtgtcctcag
      361 cctctgcctt ccagacccca ggtgtcctgg agctctgcag atcagagagg ctagtactgg
      421 aagcctgggc ccatgccccc agcagccccc agcccagggg gcccccaagg ctgaacagca
      481 agctcaggat catcttggtg gtggggcagg ctcagctcac actcagcctt ggcaagtagc
      541 tccagaaact gctagtgacg ttgtcttcaa gttaaatctc aggaggaaaa gaaaatacga
      601 ggacaacaaa gagaggaagt ggcctgggcc ggcctacccg gtgggtcttg tcctgccccc
      661 caactaccct ggctggcccc acaggggccg ccaaccacac aagccagttc ctgtccctga
      721 ggacttggct cagggactct gggaatgtgg tagacatggg gtggccccac caaatgcatc
      781 cttatgggaa cctgctccct gggagccatg aaaagagcgt ggacttcgag gtggggccac
      841 aggaagtggt caggtccatc tcaggggacc tgctgcccat ccacactgct ggccaggaaa
      901 tggggggcaa ttcatgcctc ctcagcacct tcagcactgg gcggctcaaa gaagggaagg
      961 gactattctg gggtcacaca gcatgcagcc agaggccaag gcatgaggaa gtccttcatt
     1021 tccccacccc cacccacctc agatcctcca accggtttca tggcagccca gggtccagcg
     1081 gcatccagga tgctggtggg tagctgcaca gcccaggccg cgggaggttg gctgctctca
     1141 cctaacaggc ctatgtggcc ctgaccccta cctaggaagc tggggacaat ggccaaggcg
     1201 cctcccctct ctgtgcctgt ctgtccaggt gcagcataga cacagcaccc ctggggccaa
     1261 gagcacccag ccagggctgc ccccatgggt gggcagggca gtaaatgaat gagggacagg
     1321 ttgggaggtg gccagccccc tccagcccat ggagggcacg gggcaggaga gctgggctga
     1381 gccagcagga gcccagggag cctggtctct gccttcctat cctggaggaa ggtgaggctg
     1441 aacctccttc cctccctccc tccctccccg cccccactgc acgcagggct ggctgggctc
     1501 cagctggcct ccgcatcaat atttcatcgg cgtcaatagg aggcatcggg gacagccgct
     1561 gcggcagcac tcgagccagc tcaagcccgc agctcgcagg gagatccagc tccgtcctgc
     1621 ctgcagcagc acaaccctgc acacccacca tggatgtgaa ttcatggtga gcaagggcga
     1681 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1741 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1801 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1861 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1921 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1981 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2041 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2101 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2161 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2221 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2281 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2341 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagatcta cgcgtggtac
     2401 ctctagagtc gacccgggcg gcctcgagaa gcttatcgat aatcaacctc tggattacaa
     2461 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2521 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2581 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2641 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2701 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2761 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2821 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2881 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2941 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3001 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgactcgctg atcagcctcg
     3061 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3181 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3241 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3301 agaaccagct ggggctcgac tagagcatgg ctacgtagat aagtagcatg gcgggttaat
     3361 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3421 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3481 agtgagcgag cgagcgcgca g
//
