LOCUS       Exported                2723 bp ds-DNA     linear   UNA 06-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
     misc_feature    1..131
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    186..308
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     primer_bind     186..205
                     /label=PCF
                     /note="color: black; sequence: CCATTGACGTCAATAATGAC"
     misc_feature    309..878
                     /label=hSyn1 promoter
                     /note="color: #a6acb3"
     misc_feature    886..1605
                     /label=EGFP
                     /note="color: #a6acb3"
     primer_bind     complement(919..936)
                     /label=PGR
                     /note="color: black; sequence: GACCAGGATGGGCACCAC"
     misc_feature    1657..2262
                     /label=WPRE
                     /note="WPRE color: #00ff80"
                     /note="color: #a6acb3"
     primer_bind     complement(1911..1930)
                     /label=PWR
                     /note="color: black; sequence: GCTGACAGGTGGTGGCAATG"
     misc_feature    2263..2442
                     /label=BGH polyA
                     /note="BGH polyA color: #00ffff"
                     /note="color: #a6acb3"
     misc_feature    complement(2594..2723)
                     /label=ITR(1)
                     /note="ITR color: #ff8080"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatggc ctgtgtggat gtgggagact aatctccccg cgggcactgc gtgtgacctc
      361 acccccctct gtgagggggt tatttctcta ctttcgtgtc tctgagtgtg cttccagtgc
      421 ccccctcccc ccaaaaaatg ccttctgagt tgaatatcaa cactacaaac cgagtatctg
      481 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
      541 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
      601 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
      661 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
      721 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
      781 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
      841 gatagggggg cacgggcgcg accatctgcg ctgcggcgag aattcatggt gagcaagggc
      901 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      961 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1021 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1081 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1141 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1201 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1261 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1321 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1381 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1441 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1501 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1561 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagatc tacgcgtggt
     1621 acctctagag tcgacccggg cggcctcgag aagcttatcg ataatcaacc tctggattac
     1681 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1741 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1801 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1861 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1921 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     1981 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2041 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2101 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2161 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2221 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgactcgc tgatcagcct
     2281 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2341 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2401 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2461 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     2521 aaagaaccag ctggggctcg actagagcat ggctacgtag ataagtagca tggcgggtta
     2581 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     2641 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     2701 tcagtgagcg agcgagcgcg cag
//
