LOCUS Exported 3537 bp ds-DNA linear UNA 05-MAY-2020 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3537) AUTHORS Yang Hu Lab TITLE Direct Submission JOURNAL Exported May 5, 2020 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3537 /organism="unspecified" /mol_type="genomic DNA" /note="color: #ffffff" misc_feature 1..131 /label=ITR /note="color: #a6acb3" misc_feature 186..308 /label=CMV enhancer /note="color: #a6acb3" primer_bind 186..205 /label=PCF /note="color: black; sequence: CCATTGACGTCAATAATGAC" misc_feature 309..1693 /label=hIsl2 promoter /note="color: #a6acb3" misc_feature 1700..2419 /label=EGFP /note="color: #a6acb3" primer_bind complement(1733..1750) /label=PGR /note="color: black; sequence: GACCAGGATGGGCACCAC" misc_feature 2471..3076 /label=WPRE /note="WPRE color: #00ff80" /note="color: #a6acb3" primer_bind complement(2725..2744) /label=PWR /note="color: black; sequence: GCTGACAGGTGGTGGCAATG" misc_feature 3077..3256 /label=BGH polyA /note="BGH polyA color: #00ffff" /note="color: #a6acb3" misc_feature complement(3408..3537) /label=ITR(1) /note="ITR color: #ff8080" /note="color: #a6acb3; direction: LEFT" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct 181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 301 atcatatgca ggcgcacacc aagttcttgg tactcctttt aatgggaaaa caagaacaga 361 caccatggtt ggataaatgt tatttataat tcatgggccg gtctttgaac cctttcaaac 421 aaaacccaca gcgatttctt tgaaagaatg cctggactgt gctcagagct ctcagatttt 481 ctcaggacca acgagccgcc gcctcgcaga taccactgta aattacccag cgccttacgt 541 tcgtttctga ttatttgcat tgcagtgggt ttgttaatga aggacaagag tttagaaaaa 601 tcttttattt tggaagttgg acacgcaatc gaccccagca gtgttcctaa aaggagctat 661 gccatttgga tgggggaaaa atgagggggc ggggaagtcc atgttacatt taaaacaaca 721 acagcaacaa attaaaagca aaacaaaaca acaaaaaaaa ggaagtggag caggaggagg 781 aggtggtggt ggaggagagg gaggaggggc agcagcagcg gcagcagcag caaggatgga 841 gtcaggaccc gtcccagtcc cgggtgagcg cttttggatc tgggttctgg ctcaacccac 901 gtaatgcccc aatctaaagt tttctacgtg tgtgtggata ggaaagtcta aatagagttt 961 atcagaaccc aggtggtcat tctctacact ctctctagct tggacccgaa gaacaaaggc 1021 acatgagagg agaaaggtga ccagctggac gatgacggtc acgtccaaac ggatccccct 1081 tgtcctcggc tctctcgtcc tgagagtggg tttaattttt ttttttttaa tttatataag 1141 aagggagctc ttaaaggaga gccatctcca ccctcaggta tccatcaccc agctccagcg 1201 cgcctgggaa aaccgccccg aaagtccaag aggaagctca gagttgtaac ggccgcggag 1261 ccagctcggc ggtgacgcaa ggtccagtcc agattgccag gcccggggca tgagagagga 1321 tccttgtagg tttcggaggt gggggggctg cactccattg ttcactccgg gccaatcagg 1381 gttggcccac ttcctcccag ccaatctccc ttcaccccca gcctccaacc caacccaccc 1441 cgcccatcag cccctggatc cccatcacct cccccgcatc cccggcagtt ctggggaagc 1501 ttcgtgacgc cacaggtccc gcccccagct ccggcccggg gctagtgcgt gttgacgtca 1561 tgctgcgtgc gggccggtgc ggaatcgctc cttcaactcc gcggggcagt aggagttagt 1621 tagcaaagag ccgaggccgg gcgcgcgacc ctcgtccttc tgcccctggc cgcacacttt 1681 gcgcacatct cttgaattca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 1741 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 1801 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 1861 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 1921 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 1981 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 2041 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 2101 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 2161 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 2221 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 2281 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 2341 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 2401 ggacgagctg tacaagtaaa gatctacgcg tggtacctct agagtcgacc cgggcggcct 2461 cgagaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 2521 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 2581 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 2641 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 2701 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 2761 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 2821 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc 2881 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 2941 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 3001 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 3061 cccgcatcga taccgtcgac tcgctgatca gcctcgactg tgccttctag ttgccagcca 3121 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 3181 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 3241 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 3301 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga 3361 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt 3421 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 3481 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcag //