LOCUS       Exported                4812 bp ds-DNA     linear   UNA 05-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    px551-mSncg-0.27kb-HA-SpCas9
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Yang Hu Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2020 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
     misc_feature    1..130
                     /label=ITR
                     /note="color: #a6acb3"
     misc_feature    143..407
                     /label=mSncg 0.27kb Promoter
                     /note="color: #a6acb3"
     misc_feature    424..450
                     /label=HA
                     /note="color: #a6acb3"
     CDS             451..4620
                     /label=SpCas9
                     /note="color: #993366; direction: RIGHT"
     misc_feature    complement(4683..4812)
                     /label=ITR
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gaaagagtca gcagggcaga atagagccag taggggcccg
      181 ggccctgctc gctggtatcc ccgtgaggca tgccttctct ctggcccgcc ctccctgccc
      241 ccaccctggc ccgggctggc tgggctccag ccagcagcca cagcatcaat atttcatctg
      301 cgtcaataag aggcagtagc agcagagaca gcggctgcgg cagcactcca gtccatagct
      361 tgcagcagcc aggttccatc cttgcaaaca ccatggacgt cttcaagacc ggtcgccacc
      421 atgtacccat acgatgttcc agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg
      481 tccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      541 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      601 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      661 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      721 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      781 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
      841 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
      901 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
      961 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1021 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1081 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1141 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
     1201 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1261 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1321 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1381 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1441 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1501 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1561 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     1621 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     1681 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     1741 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     1801 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     1861 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     1921 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     1981 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2041 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2101 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2161 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2221 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2281 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2341 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2401 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2461 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2521 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     2581 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     2641 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     2701 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     2761 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     2821 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     2881 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     2941 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     3001 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3061 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3121 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3181 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3241 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3301 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3361 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3421 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3481 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3541 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     3601 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     3661 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     3721 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     3781 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     3841 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     3901 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     3961 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4021 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4081 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4141 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4201 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4261 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4321 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4381 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4441 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4501 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4561 gacctgtctc agctgggagg cgacagcccc aagaagaaga gaaaggtgga ggccagctaa
     4621 gaattcaata aaagatcttt attttcatta gatctgtgtg ttggtttttt gtgtgcggcc
     4681 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4741 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4801 gcgagcgcgc ag
//
