LOCUS       Exported               19832 bp ds-DNA     circular UNA 07-MAY-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 19832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19832
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          16312..17974
                     /organism="Arabidopsis thaliana"
                     /chromosome="3"
                     /ecotype="Columbia"
                     /mol_type="genomic DNA"
                     /label=chr 3
                     /db_xref="taxon:3702"
     source          17995..19458
                     /label=Invitrogen vector 679
                     /note="/invitrogen=679"
     CDS             77..763
                     /label=Excisionase?
                     /note="Ser-Recombinase superfamily"
     misc_feature    complement(823..1823)
                     /label=STA region
                     /note="/feature_type=Misc."
     CDS             1062..1691
                     /label=ParA
                     /note="Partitioning Protein"
     CDS             2086..3192
                     /label=pVS1 replication protein
     primer_bind     3688..3710
                     /label=pGEX_3 primer
                     /note="pGEX_3 primer"
     misc_feature    4107..4186
                     /label=ori pBR322
                     /note="ori pBR322"
     primer_bind     complement(4933..4952)
                     /label=JS1630
     CDS             complement(4954..5964)
                     /label=Spect
     primer_bind     5966..5983
                     /label=JS1629
     misc_feature    6265..6299
                     /label=T-DNA LB
                     /note="T-DNA LB"
     misc_feature    6328..6351
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pICH41780 4 parts 
                     (3.318bpcircular)Fragment:from
                     BpiI cut at 2847^2848 to BpiI cutat 
                     2871^2872,24bpOrientation: reverse complementedPlusstrand 
                     5':   nochangePlus strand 3':   no changeMinusstrand 5': 
                     nochangeMinus strand 3':  no change"
     primer_bind     complement(6332..6355)
                     /label=SENSE_PRM
                     /note="Sense primer used for creating PCR20788
                     link4eb+pstuf4"
     misc_feature    complement(6332..6351)
                     /label=EL4
                     /note="/vntifkey=21"
     misc_feature    6352..8692
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG635 FAST 1-4f 
                     (6.693bpcircular)Fragment:from
                     BpiI cut at 4335^4336 to BpiI cutat 
                     6676^6677,2.341bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    6356..8676
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=E11_pICSL70008 FAST
                     (4.568bpcircular)Fragment:from BsaI cut at 2247^2248 to 
                     BsaI cutat -1^1,2.321bpOrientation: reverse 
                     complementedPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(6360..6797)
                     /label=olesin Terminator (A. thaliana)
     CDS             complement(6802..7497)
                     /codon_start=1
                     /label=TagRFP (Entacmaea quadricolor)
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             complement(join(7498..7663,7904..8256))
                     /codon_start=1
                     /label=Olesin (A. thaliana)
                     /translation="MADTARGTHHDIIGRDQYPMMGRDRDQYQMSGRGSDYSKSRQIAK
                     AATAVTAGGSLLVLSSLTLVGTVIALTVATPLLVIFSPILVPALITVALLITGFLSSGG
                     FGIAAITVFSWIYKYATGEHPQGSDKLDSARMKLGSKAQDLKDRAQYYGQQHTGGEHDR
                     DRTRGGQHTT"
     intron          complement(7664..7903)
                     /label=Intron olesin (A.thaliana)
     5'UTR           complement(8257..8324)
                     /label=5'UTR Olesin (A. thaliana)
     misc_signal     complement(8324)
                     /label=Start of transcription
     promoter        complement(8325..8670)
                     /label=Promoter Olesin (A. thaliana)
     TATA_signal     complement(8355..8361)
                     /label=TATA box
     misc_feature    join(8677..8692,6352..6355)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47761 1-4f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    8693..9876
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG634 pnos:Bar-tnos
                     1-3f(5.536bpcircular)Fragment: from BpiI cut at 4335^4336 
                     toBpiI cutat 5519^5520, 1.184bpOrientation: 
                     originalPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     misc_feature    8697..9860
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=D11_pICSL70005 Bar
                     (3.411bpcircular)Fragment:from BsaI cut at 2247^2248 to 
                     BsaI cutat -1^1,1.164bpOrientation: reverse 
                     complementedPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(8701..8999)
                     /label=Nos terminator (Agrobacterium tumefaciens)
     CDS             complement(9000..9551)
                     /codon_start=1
                     /label=Bar (phosphinothricin N-acetyltransferase)
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9594..9860)
                     /label=Nos promoter (Agrobacterium tumefaciens)
     misc_feature    join(9861..9876,8693..8696)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47751 1-3f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    complement(9861..9864)
                     /label=GGAG 4bp overhang
                     /label=nonstandard type:  MIsc._Feature
     misc_feature    9877..17990
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK074_1-2f_pRPS5a:Cas9
                     -intronized2xNLS-trbcse9corrected (12.466bp 
                     circular)Fragment: fromBpiI cut at4335^4336 to BpiI cut
                     at 12449^12450,8.114bpOrientation:originalPlus strand 5': 
                     nochangePlus strand 3':   nochangeMinus strand 5': 
                     nochangeMinus strand 3':  nochange"
     misc_feature    9881..10518
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG416_pAGM28545 
                     (2.885bpcircular)Fragment:from
                     BsaI cut at 2247^2248 to BsaI cutat
                     -1^1,638bpOrientation: reverse complementedPlus strand5':no
                     changePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    complement(9885..10522)
                     /label=E9 ter
                     /note="/vntifkey=21"
     terminator      complement(9885..10518)
                     /label=rbcS-E9 Terminator (Pisum sativum)
     misc_feature    10519..16311
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK070 [CDS1] Cas9 -intronized 
                     2xNLS[pAGM47523](corrected) (7.862bp circular)Fragment: 
                     fromBsaI cut at209^210 to BsaI cut at 
                     6002^6003,5.793bpOrientation:reverse complementedPlus 
                     strand 5':no changePlusstrand 3':   no changeMinus strand 
                     5':  nochangeMinusstrand 3':  no change"
     CDS             complement(join(10523..10795,10905..11215,11375..11722,
                     11844..12189,12326..12622,12744..13061,13212..13486,
                     13623..13970,14095..14361,14483..14743,14845..15093,
                     15202..15435,15527..15863,15994..16314))
                     /codon_start=1
                     /label=zmCas9
                     /translation="MASSPPKKKRKVSWKMDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNE
                     MAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDK
                     ADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVD
                     AKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQL
                     SKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI
                     LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
                     PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTV
                     KQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIV
                     LTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTI
                     LDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGIL
                     QTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILK
                     EHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK
                     VREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKAT
                     AKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQV
                     NIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGR
                     KRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFD
                     TTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV"
     intron          complement(10796..10904)
                     /label=intron atipl3
                     /note="/vntifkey=15"
     intron          complement(11216..11374)
                     /label=intron atbg3
                     /note="/vntifkey=15"
     intron          complement(11723..11843)
                     /label=intron e2f58
                     /note="/vntifkey=15"
     intron          complement(12190..12325)
                     /label=intron atatp11
                     /note="/vntifkey=15"
     intron          complement(12623..12743)
                     /label=Intron Rlk7
                     /note="/vntifkey=15"
     intron          complement(13062..13211)
     intron          complement(13487..13622)
                     /label=intron atatp2
                     /note="/vntifkey=15"
     intron          complement(13971..14094)
                     /label=intron atbg11
                     /note="/vntifkey=15"
     intron          complement(14362..14482)
                     /label=intron chtog11
                     /note="/vntifkey=15"
     intron          complement(14744..14844)
                     /label=intron cdpk3
                     /note="/vntifkey=15"
     intron          complement(15094..15201)
                     /label=intron chtog16 (no BpiI)
                     /note="/vntifkey=15"
     variation       15183
                     /label=G to C (no BpiI)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     intron          complement(15436..15526)
                     /label=intron Gluc4
                     /note="/vntifkey=15"
     intron          complement(15864..15993)
                     /label=intron E2f5-4
                     /note="/vntifkey=15"
     misc_feature    16312..17974
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG603_pRPS5a
                     [Pro+5U](3.910bpcircular)Fragment: from BsaI cut at 534^535
                     toBsaI cutat 2197^2198, 
                     1.663bpOrientation:reversecomplementedPlus strand 5':
                     no changePlus strand3':no changeMinus strand 5':  no 
                     changeMinus strand 3':nochange"
     primer_bind     16312..16337
                     /label=REVERSE
     misc_feature    complement(16316..17978)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At3g11940_RPS5a-RC
                     selection(1.687bplinear)Fragment: from BsaI cut at 10^11 to
                     BsaIcut at1673^1674, 1.663bpOrientation: originalPlus 
                     strand5':no changePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     gene            complement(16316..16466)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /label=RPS5A
                     /note="One of two genes encoding the ribosomal protein
                     S5. Mutants have semi-dominant developmental phenotypes. 
                     Most cell-division processes are delayed or disturbed in 
                     the heterozygous mutant, and development is completely 
                     arrested at an early embryonic stage in the homozygous 
                     mutant."
                     /db_xref="TAIR:AT3G11940"
     mRNA            complement(16316..16427)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL264057.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:AY091376.1,INSD"
     mRNA            complement(16402..16466)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar toRNAsequence,EST:same 
                     species:CB257800.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:BX824143.1,INSD"
     gene            complement(16799..17978)
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /label=HST
                     /note="Encodes a protein involved in plastoquinone-9 
                     biosynthesis. The enzyme possesses homogentisate 
                     prenyltransferase activity and was shown to use solanesyl 
                     diphosphate, farnesyl diphosphate and 
                     geranylgeranyldiphosphate as prenyl donors, but not 
                     phytyldiphosphate. This gene At3g11945 derives from a split
                     of At3g11950, publications Tian et al (2007) and Sadre et 
                     al (2006) refer to this gene as At3g11950."
                     /db_xref="TAIR:AT3G11945"
     mRNA            complement(join(16799..17075,17168..17200,17344..17406,
                     17582..17699,17856..17948))
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate solanesyltransferase"
                     /label=HST mRNA
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
     mRNA            complement(join(16799..17075,17168..17200,17344..17406,
                     17582..17699,17856..17948))
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate solanesyltransferase"
                     /label=HST mRNA
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:DQ231060.1,INSD"
     CDS             complement(join(17001..17075,17168..17200,17344..17406,
                     17582..17699,17856..17948))
                     /codon_start=1
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate prenyltransferase"
                     /label=HST
                     /note="homogentisate prenyltransferase (HST); CONTAINS
                     InterPro DOMAIN/s: UbiA prenyltransferase 
                     (InterPro:IPR000537); BEST Arabidopsis thaliana protein 
                     match is: homogentisate phytyltransferase 1 
                     (TAIR:AT2G18950.1); Has 1367 Blast hits to 1364 proteins in
                     371 species: Archae - 252; Bacteria - 598; Metazoa -
                     1; Fungi - 4; Plants - 199; Viruses - 0; Other Eukaryotes -
                     313 (source: NCBI BLink); GO_component: GO:0009941 - 
                     chloroplast envelope [Evidence IDA] [PMID 17569077]; 
                     GO_function: GO:0010355 - homogentisate farnesyltransferase
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010356 - homogentisate geranylgeranyltransferase 
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010357 - homogentisate solanesyltransferase activity 
                     [Evidence IDA] [PMID 16989822]; GO_process: GO:0010236 - 
                     plastoquinone biosynthetic process [Evidence IMP] [PMID 
                     16989822]"
                     /db_xref="GI:332641605"
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /protein_id="AEE75126.1"
                     /translation="MTCGVHHIFCDTVCTGHCYYKGPS*C*RRSKVPNINPGNKTWSEK
                     HCIPRFWTSASKLCFSHITSFLHASGF*R*LDDSCTCDLGFRLNFPDMGTRKSKLHQGS
                     YLRILSVYMESLLRRVSVIPLPL"
     CDS             complement(join(17001..17075,17168..17200,17344..17406,
                     17582..17699,17856..17948))
                     /codon_start=1
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate prenyltransferase"
                     /label=HST
                     /note="homogentisate prenyltransferase (HST); FUNCTIONS
                     IN: homogentisate geranylgeranyltransferase activity, 
                     homogentisate farnesyltransferase activity, 
                     dimethylallyltranstransferase activity, homogentisate 
                     solanesyltransferase activity; INVOLVED IN: carotenoid 
                     biosynthetic process, plastoquinone biosynthetic process; 
                     LOCATED IN: chloroplast envelope; EXPRESSED IN: 22 plant 
                     structures; EXPRESSED DURING: 13 growth stages; CONTAINS 
                     InterPro DOMAIN/s: UbiA prenyltransferase 
                     (InterPro:IPR000537); BEST Arabidopsis thaliana protein 
                     match is: homogentisate phytyltransferase 1 
                     (TAIR:AT2G18950.1); Has 1360 Blast hits to 1357 proteins in
                     372 species: Archae - 250; Bacteria - 602; Metazoa -
                     1; Fungi - 4; Plants - 187; Viruses - 0; Other Eukaryotes -
                     316 (source: NCBI BLink); GO_component: GO:0009941 - 
                     chloroplast envelope [Evidence IDA] [PMID 17569077]; 
                     GO_function: GO:0004161 - dimethylallyltranstransferase 
                     activity [Evidence NAS]; GO_function: GO:0010355 - 
                     homogentisate farnesyltransferase activity [Evidence IDA] 
                     [PMID 16989822]; GO_function: GO:0010356 - homogentisate 
                     geranylgeranyltransferase activity [Evidence IDA] [PMID 
                     16989822]; GO_function: GO:0010357 - homogentisate 
                     solanesyltransferase activity [Evidence IDA] [PMID 
                     16989822]; GO_process: GO:0016117 - carotenoid biosynthetic
                     process [Evidence IMP] [PMID 8718624]; GO_process: 
                     GO:0010236 - plastoquinone biosynthetic process [Evidence 
                     IMP] [PMID 16989822]"
                     /db_xref="GI:332641604"
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:DQ231060.1,INSD"
                     /protein_id="AEE75125.1"
                     /translation="MTCGVHHIFCDTVCTGHCYYKGPS*C*RRSKVPNINPGNKTWSEK
                     HCIPRFWTSASKLCFSHITSFLHASGF*R*LDDSCTCDLGFRLNFPDMGTRKSKLHQGS
                     YLRILSVYMESLLRRVSVIPLPL"
     primer_bind     complement(17959..17978)
                     /label=FORWARD
     misc_feature    join(17975..17990,9877..9880)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47742 1-2f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    17991..19454
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG294_ccdB (4.200bp 
                     circular)Fragment:fromBpiI
                     cut at 442^443 to BpiI cut
                     at1906^1907,1.464bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     primer_bind     17991..18010
                     /label=1032
     CDS             18112..18771
                     /codon_start=1
                     /label=Cm(R)
                     /note="/invitrogen=1080000"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             19113..19418
                     /codon_start=1
                     /label=ccdB
                     /note="/invitrogen=1060000"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(19439..19458)
                     /label=1033
     misc_feature    join(19455..19832,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pVM_BGW selection 
                     (6.700bpcircular)Targetvector:
                     from BsaI cut at 7^8 to BsaI cutat 
                     6689^6690,6.682bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    join(19455..19832,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pJOG292_pVM_new_mod
                     consensus(7.303bpcircular)Target vector: from BpiI cut 
                     at6925^6926 toBpiI cut at 6327^6328, 
                     6.705bpOrientation:originalPlusstrand 5':   no changePlus 
                     strand 3':   nochangeMinusstrand 5':  no changeMinus strand
                     3':  nochange"
     misc_feature    complement(19539..19564)
                     /label=T-DNA RB
                     /note="T-DNA RB"
     region          19558..19583
                     /label=RB T-DNA repeat
                     /note="Match: GTAAACCTAAGAGAAAAGAGCGTTTA"
                     /note="Length of match: 26"
                     /note="Accuracy (%): 100"
                     /note="Description: pCAMBIA"
                     /note="Found at strand: Positive"
     primer_bind     complement(19562..19581)
                     /label=JS1132
     primer_bind     complement(19754..19775)
                     /label=697
     primer_bind     19773..19792
                     /label=696
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgagcga
     6361 ctaaatggag caacctactg tttttgaagt ttgggttgcc atgtaatcac ttgcaataaa
     6421 tatatgtact aatgactgca tcggcttgcg tgtctaaatt tgtagcttat tgaaaacttt
     6481 ttgtggttaa agacgaatcc agttaactaa cataagttta acaaaacaag accttttcca
     6541 tatatagacc accgttacca acttacaaag aagccaaata aagtacaaca caacacttgc
     6601 aagttacaaa aagagaggaa aaaaaacatg gtaccatttg acaacatata gaaacacaca
     6661 taaacattac ttataacaaa aagattcaaa ctcggctttt attatctccc cctgatcaaa
     6721 ttctaaacac tttattcctc ataaactaac tccccgacat tggagttatt ggactatgac
     6781 gatgacatca gtggggtaag ctcacttgtg ccccagtttg ctagggaggt cgcagtatct
     6841 ggccacagcc acctcgtgct gctcgacgta ggtttctttg tcggcctcct tgattctttc
     6901 cagtctgtgg tccacatagt agacgccggg catcttgagg ttcttagcgg gtttcttgga
     6961 tctgtatgtg gtcttgaagt tgcagatcag gtggcccccg cccacgagct tcagggccat
     7021 gtcgcttctg ccttccaggc cgccgtcagc ggggtacagc atctcggtgt tggcctccca
     7081 gccgagtgtt ttcttctgca tcacagggcc gttggatggg aagttcaccc ctctgatctt
     7141 gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc
     7201 cccatcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttaaa
     7261 gaagtcgggg atgccctggg tgtggttgat gaaggttctg ctgccgtaca tgaagctggt
     7321 agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt
     7381 ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt ggtggttgtt
     7441 cacggtgccc tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctcatagt
     7501 agtgtgctgg ccaccacgag tacggtcacg gtcatgttcc ccaccagtat gttgctgtcc
     7561 gtagtactga gctctgtctt tcagatcctg agctttgctt cccaacttca tccttgcact
     7621 gtccaacttg tctgatccct gtgggtgctc tcccgttgcg tacctattca caatcaatag
     7681 gtatcatacc aagcatttta cctatataga tgatatacac agacacattc aagctagcta
     7741 ggtcaaattt cgtatttagt taaccaaatg ttccctagaa tttgcaattt cttattgtta
     7801 catttgttat tcgaccaaaa aaattgatcc aaagctactg gctcaacaca tgcatgcaca
     7861 tattgcacaa aattatgaag taagatgata aatgtgtgct tacttgtaaa tccaagagaa
     7921 aacggttata gcggcaatgc caaaccctcc agaggaaaga aaaccggtga tgaggagtgc
     7981 aactgtgatg agagccggga caaggattgg gctgaagata acgagcagag gtgttgcaac
     8041 agtcaaagct atgacagttc caacaagggt aaggctggag agaacaagga gggaaccacc
     8101 agctgtgaca gcagttgcag ctttagcaat ctgcctagac ttggagtagt cagatcctcg
     8161 tccggacatc tggtactggt cacgatctcg gcccatcatc gggtactgat ctctgccgat
     8221 gatatcgtga tgggttcctc tagctgtatc cgccattttt ttgttcttgt ttactagaga
     8281 gaatgagatt ttgaatgaga gattgatcaa aaagatgtgt atatattttg gaagaggtag
     8341 gagtgtttgt tagatatatt aagaaaggag acgagaagtc acgtgtcaat ggagacatca
     8401 tgcaattgta ggccacgtgt taatggcgtc accatgcagc cgctgcatgt gttaacgaga
     8461 ggagtctatt caaagacacg tttgtttttt gcctagctca gaatgtggcg ttggcatcga
     8521 accgaaacat aaaacgcaaa acgaaccttt gggctcgtca gtatgggttt gggccttcaa
     8581 tagattcctt ctatacggcc cacatatcca ctattggcaa caaaattaga aaaatttagg
     8641 atttagtttt aaaatttgtt ccgcgacatt ctagaactcc ccatgggaat tcgtaaagcg
     8701 ctccaattca gcaagcgtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     8761 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     8821 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     8881 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     8941 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctagatcca gagtcccgct
     9001 caaatctcag tcacaggaag aacaggtcta ggaggaacag gaagagagaa atcaagctgc
     9061 cagaatccca catcatgcca atttccatgt ttgaaaccag cagctctaag cattccccta
     9121 ggagcataac caagagcctc atgcatcctc acagaaggat cattaggaag tccaatcaca
     9181 gcaaccacag acttgaaacc ctgagcctcc aaagacttca aaagatgagt gtaaagagta
     9241 gatccaagac cagttctttg atgcctagga gacacgtaaa cagtagactc agcagtccaa
     9301 tcgtaagcat tcctagcctt ccaaggacca gcataagcaa taccagccac ttcaccatcc
     9361 acttcagcaa caagccaagg atacctctct ctaagcctca caagatcatc agtccactct
     9421 tgtggttctt gaggctcagt cctgaaattc acagtagaag tctcaatgta atgattcaca
     9481 atagtgcaca cagcaggcat atcagcctca gtagcccttc taatatcagc aggccttctt
     9541 tcaggagaca tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat
     9601 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat tttgacaaga
     9661 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     9721 cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg
     9781 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     9841 gtgactccct taattctccg ctccgagctc gaattctagt agcgttgtca atcaattggc
     9901 aagtcataaa atgcattaaa aaatattttc atactcaact acaaatccat gagtataact
     9961 ataattataa agcaatgatt agaatctgac aaggattctg gaaaattaca taaaggaaag
    10021 ttcataaatg tctaaaacac aagaggacat acttgtattc agtaacattt gcagcttttc
    10081 taggtctgaa aatatatttg ttgcctagtg aataagcata atggtacaac tacaagtgtt
    10141 ttactcctca tattaacttc ggtcattaga ggccacgatt tgacacattt ttactcaaaa
    10201 caaaatgttt gcatatctct tataatttca aattcaacac acaacaaata agagaaaaaa
    10261 caaataatat taatttgaga atgaacaaaa ggaccatatc attcattaac tcttctccat
    10321 ccatttccat ttcacagttc gatagcgaaa accgaataaa aaacacagta aattacaagc
    10381 acaacaaatg gtacaagaaa aacagttttc ccaatgccat aatactcaaa ctcagtagga
    10441 ttctggtgtg tgcgcaatga aactgatgca ttgaacttga cgaacgttgt cgaaaccgat
    10501 gatacgaacg aaagctctaa gctcagactt tacgcttctt ctttgggtca gccctagagt
    10561 caccacccaa ctgcgacaga tctatccttg tctcatagag ccctgtaata gattggtgaa
    10621 taagggtagc gtccaacact tccttggttg atgtgtagcg ctttctgtcg atagtggtat
    10681 cgaagtactt aaaggctgct ggggctccca gattggtcag tgtgaacagg tggatgatgt
    10741 tttccgcttg ttcccttatc ggcttatctc tgtgcttgtt gtacgcggaa agcaccttta
    10801 attgtagaaa accagtaaaa catggacaat taggaggcat cgattatcta accaaagact
    10861 ttaacaacac taatctgcca ctaatattca tgagaagtcc ttaccttatc gaggttagcg
    10921 tctgcaagaa tgacccgctt ggaaaattcg ctgatctgct ctatgatctc atcaagatag
    10981 tgcttgtgct gctcgacaaa caactgcttc tgctcgttgt cctctggtga ccccttcagc
    11041 ttctcatagt gtgaggccaa gtacaggaag ttgacgtact tactgggcaa tgccagctcg
    11101 ttgcccttct gcagttctcc ggcagatgcc agcatgcgtt ttctgccatt ttccaattcg
    11161 aaaagcgaat acttgggcag tttgataatg aggtctttct tcacctcttt gtaacctggt
    11221 ttgtataatt ttggttcagt taattaggaa aattacataa tggctccaaa aaaaaattat
    11281 cgacctttta ccaaaaaaaa taaaatttat cgaccttgac aggaaaaagt tacaaatcaa
    11341 aagaaagaat atcgtctggc atccaatatt ttacctttag cctcgaggaa gtcgatcgga
    11401 ttcttctcga acgaggacct ttccattatg gtaattccaa gcagttcctt gacgcttttc
    11461 agtttcttgc tcttgccctt ctccaccttg gccactacca gtacgctgta tgccacggta
    11521 gggctatcaa agcctccgta tttcttcgga tcccaatcct tcttcctcgc aatcagtttg
    11581 tcggagttgc gcttagggag aatggactcc ttggagaaac caccggtttg aacctccgtt
    11641 ttcttgacta tgttgacttg gggcatgctg agaaccttac gcacagtcgc aaaatccctg
    11701 cccttatccc agacaatctc acctgcaaga aacagcaaag agagagttaa gatataagtg
    11761 aacaagaaag tcttgggatc gacatacata caatcaattg atcaaattcc taggattaaa
    11821 catatgaaat aggaaagaca gacctgtttc gccatttgtc tcgatcagag gcctcttccg
    11881 gatttcacca ttcgccagag tgatctctgt cttgaagaag ttcatgatat tcgagtaaaa
    11941 gaagtatttc gcggtagcct tgccgatctc ttgttctgac ttcgcgatca tcttcctaac
    12001 gtcgtacacc ttgtaatccc cgtacacaaa ctcagactcc agtttcggat acttcttgat
    12061 gagagcagtg ccaacaacgg cgttcaggta ggcgtcatgg gcatgatggt agttgttgat
    12121 ctcgcgaacc ttgtaaaact ggaagtcctt ccggaagtca ctaaccagtt tgctcttgag
    12181 ggtgatcacc tgcgtaacca tcaaaagtca atatggattc atcacaaatt aatcaggaat
    12241 agaaagatcg ggtggccttg aagttgatca aataaaaagt ttcgaacttt agtttgatat
    12301 ggtaaaggaa agttggaaac tttaccttta cctccctgat aagtttgtca ttctcatcat
    12361 acttcgtatt catacgcgaa tcgagtatct gggccacatg tttcgtaatc tgacgagttt
    12421 caaccaactg gcgcttgatg aagccggctt tgtcgagctc agacagtccg cccctttctg
    12481 ccttcgtgag attgtcgaat ttccgttggg ttatgagctt agcattcaaa agctgcctcc
    12541 aatagttctt catcttcttc acaacctcct cgctgggtac attgtcggat tttccgcggt
    12601 tcttgtctga gcgcgtaagg acctataaca cgggagaaac caaaaagcaa actcatttca
    12661 cgccaaatac tacagatttc tcaaaaatta agttataatc catatatcct gacaagaaat
    12721 tgattaaaac acagttgctt taccttgttg tcaatactat cgtccttcag gaaagattga
    12781 gggacaatgt gatccacatc atagtcggag agtctgttaa tgtcgagctc ttggtcgacg
    12841 tacatatctc tcccattctg gaggtaatag aggtacagct tctcgttctg gagttgagta
    12901 ttttcgactg ggtgctcttt cagaatctga ctcccaagct ccttgatgcc ttcctctatc
    12961 cgcttcatgc gctcccgtga gttcttctgc cctttctgag tcgtctggtt ttccctcgcc
    13021 atctcgatga cgatgttctc cggcttatgg cggcccatga cctgtagcaa taattaaaac
    13081 aaaaatacca caaacagtta gaactgaggc aagaagaatg aatgcagaga aaaaaaatct
    13141 tagctattca tggaaatgga agtgcgacga acacctaaaa tgcaaggagc ataaccaaat
    13201 gcagaactta ccttcaccag ctcatccaca accttcactg tctgcagtat gcccttcttt
    13261 atagctggcg atccagccag gttggctatg tgttcgtgaa ggctatcgcc ctgacctgac
    13321 acttgggcct tctggatatc ttctttgaag gtcagtgagt catcgtgaat aagctgcatg
    13381 aagttccgat tcgcaaaacc gtcggatttg agaaagtcca gtatagtctt gccggattgc
    13441 ttgtcgcgga tgccgttgat gagcttcctg gagagccttc cccaacctaa aacatagaac
    13501 aaacatttgt ctcatcacaa atatgcatca aggaacactc agaagttagt catataccca
    13561 aattcttgaa actagagaaa caaaaaaaga gggatcattg cggaaacctt ttgacctctt
    13621 acctgtatat ctcctcctct tcaactgctt catgaccttg tcatcgaaca gatgcgcata
    13681 agtcttaagg cgctcctcga tcatttctct gtcctcaaac agcgtgagag tcagcacgat
    13741 gtcctcgagg atgtcctcgt tctcctcgtt gtcaaggaag tccttgtcct tgatgatttt
    13801 caacaggtca tggtatgtgc caagagaggc attgaaccta tcctcaacgc cgctgatttc
    13861 cacagaatca aagcattcga tcttcttaaa gtagtcctct ttaagctgtt tcacagtgac
    13921 cttccgattg gttttgaaca agagatcgac aatggccttc ttctgctcac ctgttataac
    13981 agtaaaaatt actttagaaa caaaacaaaa gtgtggtctt ttaggaatga ttattgatca
    14041 gttcatagtt taaaatcttg tgtttttatc gtaaaagtgt ggaccaggat ttacctgaca
    14101 ggaaggccgg tttgcgcatc ccctccgtca catatttcac cttcgtcagc tcgttgtaga
    14161 ccgtgaagta ttcgtacagg agactatgct tgggcaggac tttctcattt gggagattct
    14221 tatcgaagtt ggtcatcctt tcgatgaagg actgtgcact cgcacccttg tccacgacct
    14281 cttcgaagtt ccaaggcgtg atagtctcct cggatttgcg tgtcatccac gcaaaccgcg
    14341 agttcccgcg tgcgagtgga cctaatgaat taatagaaaa atcaggcaaa ttagaaaaga
    14401 aataattctc aaaagagaac aactgaggtc agaaaatagc attcatcaac tgaaactagg
    14461 tatttcttca tatgatacta acctacatag tagggaatgc ggaacgtgag gatcttttca
    14521 atcttctctc tgttgtcttt caggaacgga tagaagtcct cttgcctcct caagatagca
    14581 tgcagctcac cgaggtggat ctggtgcggt atgctgccat tgtcgaaggt acgctgcttc
    14641 cgaagcaaat cttcgcggtt caatttaacg agcagctcct ctgtaccgtc catcttttcc
    14701 agaataggct tgatgaattt gtagaattcc tcctgacttg cacctaataa tatcatcaga
    14761 ggaaaactca attcttatat cttacagaaa tgaacaacaa aatacatgta gcagagagga
    14821 aaacaatcat aagtaacaac ttacctccat caatgtaccc tgcgtagcca ttcttcgatt
    14881 ggtcaaagaa aatctccttg tacttctcgg ggagttgctg gcgaaccaaa gccttcagaa
    14941 gggtcagatc ctggtgatgc tcgtcgtacc tcttgatcat ggaggcgcta agtggcgctt
    15001 tggttatctc cgtgttgacc cgcagaatgt cactgagcaa gatagcgtct gacagattct
    15061 tggcagccaa gaacaggtcg gcatactgat cacctacaaa agtttaaaag actcaatttt
    15121 aactctacag cttcaaataa atttccgcaa agaaattgat aggtttagac aacaaaagaa
    15181 gagttccaat ttcaagagca cctatttggg cgagcagatt gtccagatca tcatcgtatg
    15241 tatcctttga cagctgcagc ttcgcgtcct cggcgaggtc aaagttgctt ttgaagttag
    15301 gggtgaggcc cagagacagt gcaatcagat tcccgaacaa gccgttcttc ttctcgcctg
    15361 gcaattgagc gatcagattc tcaagccgtc tggacttcga aagtctggca gacagtattg
    15421 ccttggcgtc gacacctgag attgtttgca aagaaaaatc tcaaagcatg tacaatctat
    15481 aaacttatga tggaaaaaga aaagaaaggt aactaaggaa tgttacctga tgcattgatg
    15541 gggttctcct cgaacagctg attgtaggtt tggacgagct gaatgaacag tttatccaca
    15601 tcggagttat ccgggttgag gtccccttcg atcaagaaat ggccacgaaa ctttatcatg
    15661 tgcgcgagag ccaggtatat gagccgaagg tctgccttat cggttgagtc caccagcttc
    15721 ttgcgaaggt ggtatatagt cggatacttt tcatgatagg ctacttcgtc aacaatattc
    15781 ccaaagatgg ggtgacgctc gtgtttctta tcttcctcta ccaggaagga ttcctcgagg
    15841 cgatggaaga acgagtcgtc aacctgcaaa atcaagttta ttgatcatca agtgagttta
    15901 catgtagtta aatgctttca gtaatatccc aatgaagcag gatatgcttt cagtacaaaa
    15961 tacataagca tactatatca taaaaatcct taccttcgcc atctcgttgc taaagatctc
    16021 ttggagataa cagattctgt tcttcctgcg agtgtacctt ctcctcgcag tacgtttaag
    16081 ccttgtcgcc tccgcggttt ctccagagtc aaagagcaga gctccgatca agttcttctt
    16141 aatgctgtgc ctatctgtgt tgcccagcac tttaaatttc ttcgaaggta ccttgtattc
    16201 atcggtgata acggcccacc caactgagtt cgtaccaata tcaagtccaa tgctgtactt
    16261 cttgtccatt ttccagctga ccttccgctt cttcttcggt gggctagaag ccattgctgt
    16321 ggtgagagaa acagagcgtg agctcaaata cacaagtacg aaatcagagt tatgagagag
    16381 gatttggaga aagaagatca cctgaagatc gtttgcgtcg cgcgaatgag agagacggca
    16441 gagaaaacga gaagctttaa gaggacgaac aaaaacccta atgtcactcc ttttatatag
    16501 atgagaaatt tagggtttgg gctctaatgg agctgtctac atgggctttc atttctttat
    16561 ccagtaaaga accggtttaa caggttccct ggtttatacg tggttttagc cggttaactt
    16621 acggaatatt atttttatct taagttacac aaattacatc attaatagta aaacaaaatt
    16681 ttatggtgca atttaagttt ttatgatgag tgaaattcga ataatataca aatattttac
    16741 tgtttaggtt tccctaaacc tacttttaga tatgtaaatt aaacattttt ttttttttgg
    16801 ttagaaccaa gtcagtgatc atagaactca aaaagctggt gtttcaattg gtacaagcat
    16861 ttttattgca agatataaaa attcagaagg acatatcctt gatgtatgta catgaaggaa
    16921 ccgattttga ctccaagcta caaagaagaa gaagaatgat atacaaagaa aactgcatat
    16981 cctcaccatg aaattgaaag ctagaggaag gggaataaca gatactctgc gtagaagaga
    17041 ttccatataa accgataata tcctgagata gcttcctgca ataatcaaat gtttctacac
    17101 tccgttagat atacactcca ttgatgttcc gtgataacga gtttatgttt gtacaaaagt
    17161 attttacctt ggtgtagttt gctttttcta gtacccatgt ctaccaaaga tcaacaacaa
    17221 agatattaga gattaatgaa tttggcagtg tcaaatgcag aaactaaagc atatagttat
    17281 gaagttcttg tgcattatct caaaagtatg taagagatat aagagtatct cataatgctt
    17341 tacctggaaa attaagcctg aagccaagat cacatgtgca ggaatcatca agctacctct
    17401 aaaaacctgt tcattgttaa aggataagag ggttggtctt ttcaaagctg catactgagg
    17461 aaagtaacaa tatcgcaaca aaaataaatt ggtggcgata gtttatcaag cttctacatg
    17521 cagtaatggt gttcgtgctt taattaaccg aaatagttaa gacggaaata gatgaattca
    17581 cctgaggcat gtagaaagct agtgatatgg ctgaaacata atttactagc agaagtccag
    17641 aaccgaggaa tgcaatgttt ctcactccaa gttttgttgc cagggttgat atttggaacc
    17701 tattcaagat caatcaaagg catgattaac taaacatgct tgaacaaaca cataaagaca
    17761 atatatcaaa cggagctggt taaacattgt ctttgactgt caaatggatt gagccaaaga
    17821 gcggaaagaa aatggttatg cgtgcagtta cttactttcg atctccttca acatcaggaa
    17881 ggtcctttgt aatagcaatg accagtgcaa acagtgtcac aaaagatgtg atgaacgcca
    17941 caggtgcact gcaaatagcg aatcaaaagt tgagctccaa gcttgaattc ttgcagagac
    18001 cgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
    18061 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
    18121 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
    18181 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
    18241 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
    18301 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
    18361 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
    18421 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
    18481 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
    18541 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
    18601 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
    18661 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
    18721 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atcgcgtgga
    18781 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
    18841 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
    18901 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
    18961 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
    19021 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
    19081 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
    19141 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
    19201 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
    19261 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
    19321 gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
    19381 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
    19441 gccagtcggt ctcagcttct gcaggcatgc gacgtcgagc ttagcttgag cttggatcag
    19501 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
    19561 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
    19621 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
    19681 actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt
    19741 gcagtcggct tctgacgttc agtgcagccg tgttctgaaa acgacatgtc gcacaagtcc
    19801 taagttacgc gacaggctgc cgccctgccc tt
//
