LOCUS       Exported               20082 bp ds-DNA     circular UNA 07-MAY-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20082
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          16562..18224
                     /organism="Arabidopsis thaliana"
                     /chromosome="3"
                     /ecotype="Columbia"
                     /mol_type="genomic DNA"
                     /label=chr 3
                     /db_xref="taxon:3702"
     source          18245..19708
                     /label=Invitrogen vector 679
                     /note="/invitrogen=679"
     CDS             77..763
                     /label=Excisionase?
                     /note="Ser-Recombinase superfamily"
     misc_feature    complement(823..1823)
                     /label=STA region
                     /note="/feature_type=Misc."
     CDS             1062..1691
                     /label=ParA
                     /note="Partitioning Protein"
     CDS             2086..3192
                     /label=pVS1 replication protein
     primer_bind     3688..3710
                     /label=pGEX_3 primer
                     /note="pGEX_3 primer"
     misc_feature    4107..4186
                     /label=ori pBR322
                     /note="ori pBR322"
     primer_bind     complement(4933..4952)
                     /label=JS1630
     CDS             complement(4954..5964)
                     /label=Spect
     primer_bind     5966..5983
                     /label=JS1629
     misc_feature    6265..6299
                     /label=T-DNA LB
                     /note="T-DNA LB"
     misc_feature    6328..6351
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pICH41780 4 parts 
                     (3.318bpcircular)Fragment:from
                     BpiI cut at 2847^2848 to BpiI cutat 
                     2871^2872,24bpOrientation: reverse complementedPlusstrand 
                     5':   nochangePlus strand 3':   no changeMinusstrand 5': 
                     nochangeMinus strand 3':  no change"
     primer_bind     complement(6332..6355)
                     /label=SENSE_PRM
                     /note="Sense primer used for creating PCR20788
                     link4eb+pstuf4"
     misc_feature    complement(6332..6351)
                     /label=EL4
                     /note="/vntifkey=21"
     misc_feature    6352..8692
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG635 FAST 1-4f 
                     (6.693bpcircular)Fragment:from
                     BpiI cut at 4335^4336 to BpiI cutat 
                     6676^6677,2.341bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    6356..8676
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=E11_pICSL70008 FAST
                     (4.568bpcircular)Fragment:from BsaI cut at 2247^2248 to 
                     BsaI cutat -1^1,2.321bpOrientation: reverse 
                     complementedPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(6360..6797)
                     /label=olesin Terminator (A. thaliana)
     CDS             complement(6802..7497)
                     /codon_start=1
                     /label=TagRFP (Entacmaea quadricolor)
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             complement(join(7498..7663,7904..8256))
                     /codon_start=1
                     /label=Olesin (A. thaliana)
                     /translation="MADTARGTHHDIIGRDQYPMMGRDRDQYQMSGRGSDYSKSRQIAK
                     AATAVTAGGSLLVLSSLTLVGTVIALTVATPLLVIFSPILVPALITVALLITGFLSSGG
                     FGIAAITVFSWIYKYATGEHPQGSDKLDSARMKLGSKAQDLKDRAQYYGQQHTGGEHDR
                     DRTRGGQHTT"
     intron          complement(7664..7903)
                     /label=Intron olesin (A.thaliana)
     5'UTR           complement(8257..8324)
                     /label=5'UTR Olesin (A. thaliana)
     misc_signal     complement(8324)
                     /label=Start of transcription
     promoter        complement(8325..8670)
                     /label=Promoter Olesin (A. thaliana)
     TATA_signal     complement(8355..8361)
                     /label=TATA box
     misc_feature    join(8677..8692,6352..6355)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47761 1-4f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    8693..10126
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG633 pnos:nptII-tnos
                     1-3f(5.786bpcircular)Fragment: from BpiI cut at 4335^4336 
                     toBpiI cutat 5769^5770, 1.434bpOrientation: 
                     originalPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     misc_feature    8697..8963
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=H11_pICH41421 nos 
                     (2.514bpcircular)Fragment:from
                     BsaI cut at 2231^2232 to BsaI cutat 
                     2498^2499,267bpOrientation: reverse
                     complementedPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(8701..8963)
                     /label=nost
                     /note="/vntifkey=43"
     misc_feature    8964..9759
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG351_nptII_CDS1
                     (3.043bpcircular)Fragment:from BsaI cut at 323^324 to
                     BsaI cut at1119^1120,796bpOrientation: reverse 
                     complementedPlusstrand 5':no changePlus strand 3':   no 
                     changeMinusstrand 5':  nochangeMinus strand 3':  no change"
     primer_bind     8964..8988
                     /label=R
     CDS             complement(8964..8967)
                     /codon_start=1
                     /label=GCTT
                     /note="/vntifkey=4"
                     /translation="A"
     misc_feature    complement(8968..9763)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pICH86966 selection (822bp
                     linear)Fragment:fromBpiI cut at 11^12 to BpiI cut 
                     at807^808,796bpOrientation: originalPlus strand 5': 
                     nochangePlusstrand 3':   no changeMinus strand 5': 
                     nochangeMinusstrand 3':  no change"
     CDS             complement(8968..9762)
                     /codon_start=1
                     /label=Kan
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9742..9763)
                     /label=F
     misc_feature    9760..10110
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=D3_pICH87633_nos (2.598bp
                     circular)Fragment:fromBsaI cut at 2247^2248 to BsaI cut 
                     at-1^1,351bpOrientation: reverse complementedPlus 
                     strand5':no changePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     5'UTR           complement(9764..9830)
                     /label=TMV Omega
                     /label=omega (TMV)
                     /note="/vntifkey=52"
     promoter        complement(9835..10110)
                     /label=nos promoter (A. tumefaciens)
                     /label=nos-p
                     /note="/vntifkey=29"
     misc_feature    join(10111..10126,8693..8696)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47751 1-3f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    complement(10111..10114)
                     /label=GGAG 4bp overhang
     misc_feature    10127..18240
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK074_1-2f_pRPS5a:Cas9
                     -intronized2xNLS-trbcse9corrected (12.466bp 
                     circular)Fragment: fromBpiI cut at4335^4336 to BpiI cut
                     at 12449^12450,8.114bpOrientation:originalPlus strand 5': 
                     nochangePlus strand 3':   nochangeMinus strand 5': 
                     nochangeMinus strand 3':  nochange"
     misc_feature    10131..10768
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG416_pAGM28545 
                     (2.885bpcircular)Fragment:from
                     BsaI cut at 2247^2248 to BsaI cutat
                     -1^1,638bpOrientation: reverse complementedPlus strand5':no
                     changePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    complement(10135..10772)
                     /label=E9 ter
                     /note="/vntifkey=21"
     terminator      complement(10135..10768)
                     /label=rbcS-E9 Terminator (Pisum sativum)
     misc_feature    10769..16561
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK070 [CDS1] Cas9 -intronized 
                     2xNLS[pAGM47523](corrected) (7.862bp circular)Fragment: 
                     fromBsaI cut at209^210 to BsaI cut at 
                     6002^6003,5.793bpOrientation:reverse complementedPlus 
                     strand 5':no changePlusstrand 3':   no changeMinus strand 
                     5':  nochangeMinusstrand 3':  no change"
     CDS             complement(join(10773..11045,11155..11465,11625..11972,
                     12094..12439,12576..12872,12994..13311,13462..13736,
                     13873..14220,14345..14611,14733..14993,15095..15343,
                     15452..15685,15777..16113,16244..16564))
                     /codon_start=1
                     /label=zmCas9
                     /translation="MASSPPKKKRKVSWKMDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNE
                     MAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDK
                     ADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVD
                     AKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQL
                     SKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRY
                     DEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKI
                     LTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
                     PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTV
                     KQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIV
                     LTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTI
                     LDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGIL
                     QTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILK
                     EHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK
                     VREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKAT
                     AKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQV
                     NIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGR
                     KRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFD
                     TTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV"
     intron          complement(11046..11154)
                     /label=intron atipl3
                     /note="/vntifkey=15"
     intron          complement(11466..11624)
                     /label=intron atbg3
                     /note="/vntifkey=15"
     intron          complement(11973..12093)
                     /label=intron e2f58
                     /note="/vntifkey=15"
     intron          complement(12440..12575)
                     /label=intron atatp11
                     /note="/vntifkey=15"
     intron          complement(12873..12993)
                     /label=Intron Rlk7
                     /note="/vntifkey=15"
     intron          complement(13312..13461)
     intron          complement(13737..13872)
                     /label=intron atatp2
                     /note="/vntifkey=15"
     intron          complement(14221..14344)
                     /label=intron atbg11
                     /note="/vntifkey=15"
     intron          complement(14612..14732)
                     /label=intron chtog11
                     /note="/vntifkey=15"
     intron          complement(14994..15094)
                     /label=intron cdpk3
                     /note="/vntifkey=15"
     intron          complement(15344..15451)
                     /label=intron chtog16 (no BpiI)
                     /note="/vntifkey=15"
     variation       15433
                     /label=G to C (no BpiI)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     intron          complement(15686..15776)
                     /label=intron Gluc4
                     /note="/vntifkey=15"
     intron          complement(16114..16243)
                     /label=intron E2f5-4
                     /note="/vntifkey=15"
     misc_feature    16562..18224
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG603_pRPS5a
                     [Pro+5U](3.910bpcircular)Fragment: from BsaI cut at 534^535
                     toBsaI cutat 2197^2198, 
                     1.663bpOrientation:reversecomplementedPlus strand 5':
                     no changePlus strand3':no changeMinus strand 5':  no 
                     changeMinus strand 3':nochange"
     primer_bind     16562..16587
                     /label=REVERSE
     misc_feature    complement(16566..18228)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At3g11940_RPS5a-RC
                     selection(1.687bplinear)Fragment: from BsaI cut at 10^11 to
                     BsaIcut at1673^1674, 1.663bpOrientation: originalPlus 
                     strand5':no changePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     gene            complement(16566..16716)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /label=RPS5A
                     /note="One of two genes encoding the ribosomal protein
                     S5. Mutants have semi-dominant developmental phenotypes. 
                     Most cell-division processes are delayed or disturbed in 
                     the heterozygous mutant, and development is completely 
                     arrested at an early embryonic stage in the homozygous 
                     mutant."
                     /db_xref="TAIR:AT3G11940"
     mRNA            complement(16566..16677)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL264057.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:AY091376.1,INSD"
     mRNA            complement(16652..16716)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar toRNAsequence,EST:same 
                     species:CB257800.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:BX824143.1,INSD"
     gene            complement(17049..18228)
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /label=HST
                     /note="Encodes a protein involved in plastoquinone-9 
                     biosynthesis. The enzyme possesses homogentisate 
                     prenyltransferase activity and was shown to use solanesyl 
                     diphosphate, farnesyl diphosphate and 
                     geranylgeranyldiphosphate as prenyl donors, but not 
                     phytyldiphosphate. This gene At3g11945 derives from a split
                     of At3g11950, publications Tian et al (2007) and Sadre et 
                     al (2006) refer to this gene as At3g11950."
                     /db_xref="TAIR:AT3G11945"
     mRNA            complement(join(17049..17325,17418..17450,17594..17656,
                     17832..17949,18106..18198))
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate solanesyltransferase"
                     /label=HST mRNA
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
     mRNA            complement(join(17049..17325,17418..17450,17594..17656,
                     17832..17949,18106..18198))
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate solanesyltransferase"
                     /label=HST mRNA
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:DQ231060.1,INSD"
     CDS             complement(join(17251..17325,17418..17450,17594..17656,
                     17832..17949,18106..18198))
                     /codon_start=1
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate prenyltransferase"
                     /label=HST
                     /note="homogentisate prenyltransferase (HST); CONTAINS
                     InterPro DOMAIN/s: UbiA prenyltransferase 
                     (InterPro:IPR000537); BEST Arabidopsis thaliana protein 
                     match is: homogentisate phytyltransferase 1 
                     (TAIR:AT2G18950.1); Has 1367 Blast hits to 1364 proteins in
                     371 species: Archae - 252; Bacteria - 598; Metazoa -
                     1; Fungi - 4; Plants - 199; Viruses - 0; Other Eukaryotes -
                     313 (source: NCBI BLink); GO_component: GO:0009941 - 
                     chloroplast envelope [Evidence IDA] [PMID 17569077]; 
                     GO_function: GO:0010355 - homogentisate farnesyltransferase
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010356 - homogentisate geranylgeranyltransferase 
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010357 - homogentisate solanesyltransferase activity 
                     [Evidence IDA] [PMID 16989822]; GO_process: GO:0010236 - 
                     plastoquinone biosynthetic process [Evidence IMP] [PMID 
                     16989822]"
                     /db_xref="GI:332641605"
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /protein_id="AEE75126.1"
                     /translation="MTCGVHHIFCDTVCTGHCYYKGPS*C*RRSKVPNINPGNKTWSEK
                     HCIPRFWTSASKLCFSHITSFLHASGF*R*LDDSCTCDLGFRLNFPDMGTRKSKLHQGS
                     YLRILSVYMESLLRRVSVIPLPL"
     CDS             complement(join(17251..17325,17418..17450,17594..17656,
                     17832..17949,18106..18198))
                     /codon_start=1
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate prenyltransferase"
                     /label=HST
                     /note="homogentisate prenyltransferase (HST); FUNCTIONS
                     IN: homogentisate geranylgeranyltransferase activity, 
                     homogentisate farnesyltransferase activity, 
                     dimethylallyltranstransferase activity, homogentisate 
                     solanesyltransferase activity; INVOLVED IN: carotenoid 
                     biosynthetic process, plastoquinone biosynthetic process; 
                     LOCATED IN: chloroplast envelope; EXPRESSED IN: 22 plant 
                     structures; EXPRESSED DURING: 13 growth stages; CONTAINS 
                     InterPro DOMAIN/s: UbiA prenyltransferase 
                     (InterPro:IPR000537); BEST Arabidopsis thaliana protein 
                     match is: homogentisate phytyltransferase 1 
                     (TAIR:AT2G18950.1); Has 1360 Blast hits to 1357 proteins in
                     372 species: Archae - 250; Bacteria - 602; Metazoa -
                     1; Fungi - 4; Plants - 187; Viruses - 0; Other Eukaryotes -
                     316 (source: NCBI BLink); GO_component: GO:0009941 - 
                     chloroplast envelope [Evidence IDA] [PMID 17569077]; 
                     GO_function: GO:0004161 - dimethylallyltranstransferase 
                     activity [Evidence NAS]; GO_function: GO:0010355 - 
                     homogentisate farnesyltransferase activity [Evidence IDA] 
                     [PMID 16989822]; GO_function: GO:0010356 - homogentisate 
                     geranylgeranyltransferase activity [Evidence IDA] [PMID 
                     16989822]; GO_function: GO:0010357 - homogentisate 
                     solanesyltransferase activity [Evidence IDA] [PMID 
                     16989822]; GO_process: GO:0016117 - carotenoid biosynthetic
                     process [Evidence IMP] [PMID 8718624]; GO_process: 
                     GO:0010236 - plastoquinone biosynthetic process [Evidence 
                     IMP] [PMID 16989822]"
                     /db_xref="GI:332641604"
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar toRNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /inference="Similar toRNAsequence,mRNA:same 
                     species:DQ231060.1,INSD"
                     /protein_id="AEE75125.1"
                     /translation="MTCGVHHIFCDTVCTGHCYYKGPS*C*RRSKVPNINPGNKTWSEK
                     HCIPRFWTSASKLCFSHITSFLHASGF*R*LDDSCTCDLGFRLNFPDMGTRKSKLHQGS
                     YLRILSVYMESLLRRVSVIPLPL"
     primer_bind     complement(18209..18228)
                     /label=FORWARD
     misc_feature    join(18225..18240,10127..10130)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47742 1-2f (4.968bp
                     circular)Targetvector:from BsaI cut at 4935^4936 to BsaI 
                     cut at4339^4340,4.372bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    18241..19704
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG294_ccdB (4.200bp 
                     circular)Fragment:fromBpiI
                     cut at 442^443 to BpiI cut
                     at1906^1907,1.464bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     primer_bind     18241..18260
                     /label=1032
     CDS             18362..19021
                     /codon_start=1
                     /label=Cm(R)
                     /note="/invitrogen=1080000"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             19363..19668
                     /codon_start=1
                     /label=ccdB
                     /note="/invitrogen=1060000"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(19689..19708)
                     /label=1033
     misc_feature    join(19705..20082,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pVM_BGW selection 
                     (6.700bpcircular)Targetvector:
                     from BsaI cut at 7^8 to BsaI cutat 
                     6689^6690,6.682bpOrientation: originalPlus strand 
                     5':nochangePlus strand 3':   no changeMinus strand 
                     5':nochangeMinus strand 3':  no change"
     misc_feature    join(19705..20082,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pJOG292_pVM_new_mod
                     consensus(7.303bpcircular)Target vector: from BpiI cut 
                     at6925^6926 toBpiI cut at 6327^6328, 
                     6.705bpOrientation:originalPlusstrand 5':   no changePlus 
                     strand 3':   nochangeMinusstrand 5':  no changeMinus strand
                     3':  nochange"
     misc_feature    complement(19789..19814)
                     /label=T-DNA RB
                     /note="T-DNA RB"
     region          19808..19833
                     /label=RB T-DNA repeat
                     /note="Match: GTAAACCTAAGAGAAAAGAGCGTTTA"
                     /note="Length of match: 26"
                     /note="Accuracy (%): 100"
                     /note="Description: pCAMBIA"
                     /note="Found at strand: Positive"
     primer_bind     complement(19812..19831)
                     /label=JS1132
     primer_bind     complement(20004..20025)
                     /label=697
     primer_bind     20023..20042
                     /label=696
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgagcga
     6361 ctaaatggag caacctactg tttttgaagt ttgggttgcc atgtaatcac ttgcaataaa
     6421 tatatgtact aatgactgca tcggcttgcg tgtctaaatt tgtagcttat tgaaaacttt
     6481 ttgtggttaa agacgaatcc agttaactaa cataagttta acaaaacaag accttttcca
     6541 tatatagacc accgttacca acttacaaag aagccaaata aagtacaaca caacacttgc
     6601 aagttacaaa aagagaggaa aaaaaacatg gtaccatttg acaacatata gaaacacaca
     6661 taaacattac ttataacaaa aagattcaaa ctcggctttt attatctccc cctgatcaaa
     6721 ttctaaacac tttattcctc ataaactaac tccccgacat tggagttatt ggactatgac
     6781 gatgacatca gtggggtaag ctcacttgtg ccccagtttg ctagggaggt cgcagtatct
     6841 ggccacagcc acctcgtgct gctcgacgta ggtttctttg tcggcctcct tgattctttc
     6901 cagtctgtgg tccacatagt agacgccggg catcttgagg ttcttagcgg gtttcttgga
     6961 tctgtatgtg gtcttgaagt tgcagatcag gtggcccccg cccacgagct tcagggccat
     7021 gtcgcttctg ccttccaggc cgccgtcagc ggggtacagc atctcggtgt tggcctccca
     7081 gccgagtgtt ttcttctgca tcacagggcc gttggatggg aagttcaccc ctctgatctt
     7141 gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc
     7201 cccatcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttaaa
     7261 gaagtcgggg atgccctggg tgtggttgat gaaggttctg ctgccgtaca tgaagctggt
     7321 agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt
     7381 ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt ggtggttgtt
     7441 cacggtgccc tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctcatagt
     7501 agtgtgctgg ccaccacgag tacggtcacg gtcatgttcc ccaccagtat gttgctgtcc
     7561 gtagtactga gctctgtctt tcagatcctg agctttgctt cccaacttca tccttgcact
     7621 gtccaacttg tctgatccct gtgggtgctc tcccgttgcg tacctattca caatcaatag
     7681 gtatcatacc aagcatttta cctatataga tgatatacac agacacattc aagctagcta
     7741 ggtcaaattt cgtatttagt taaccaaatg ttccctagaa tttgcaattt cttattgtta
     7801 catttgttat tcgaccaaaa aaattgatcc aaagctactg gctcaacaca tgcatgcaca
     7861 tattgcacaa aattatgaag taagatgata aatgtgtgct tacttgtaaa tccaagagaa
     7921 aacggttata gcggcaatgc caaaccctcc agaggaaaga aaaccggtga tgaggagtgc
     7981 aactgtgatg agagccggga caaggattgg gctgaagata acgagcagag gtgttgcaac
     8041 agtcaaagct atgacagttc caacaagggt aaggctggag agaacaagga gggaaccacc
     8101 agctgtgaca gcagttgcag ctttagcaat ctgcctagac ttggagtagt cagatcctcg
     8161 tccggacatc tggtactggt cacgatctcg gcccatcatc gggtactgat ctctgccgat
     8221 gatatcgtga tgggttcctc tagctgtatc cgccattttt ttgttcttgt ttactagaga
     8281 gaatgagatt ttgaatgaga gattgatcaa aaagatgtgt atatattttg gaagaggtag
     8341 gagtgtttgt tagatatatt aagaaaggag acgagaagtc acgtgtcaat ggagacatca
     8401 tgcaattgta ggccacgtgt taatggcgtc accatgcagc cgctgcatgt gttaacgaga
     8461 ggagtctatt caaagacacg tttgtttttt gcctagctca gaatgtggcg ttggcatcga
     8521 accgaaacat aaaacgcaaa acgaaccttt gggctcgtca gtatgggttt gggccttcaa
     8581 tagattcctt ctatacggcc cacatatcca ctattggcaa caaaattaga aaaatttagg
     8641 atttagtttt aaaatttgtt ccgcgacatt ctagaactcc ccatgggaat tcgtaaagcg
     8701 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     8761 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     8821 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     8881 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     8941 aatgtttgaa cgatctgctt gacaagctca gaagaactcg tcaagaaggc gatagaaggc
     9001 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     9061 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     9121 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     9181 cggcaagcag gcatcgccat gagtcacgac gagatcctcg ccgtcgggca tacgcgcctt
     9241 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     9301 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     9361 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     9421 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     9481 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     9541 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
     9601 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     9661 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     9721 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc attgtaattg taaatagtaa
     9781 ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatac agtatgagag
     9841 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     9901 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     9961 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
    10021 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
    10081 gtcataacgt gactccctta attctccgct ctccgagctc gaattctagt agcgttgtca
    10141 atcaattggc aagtcataaa atgcattaaa aaatattttc atactcaact acaaatccat
    10201 gagtataact ataattataa agcaatgatt agaatctgac aaggattctg gaaaattaca
    10261 taaaggaaag ttcataaatg tctaaaacac aagaggacat acttgtattc agtaacattt
    10321 gcagcttttc taggtctgaa aatatatttg ttgcctagtg aataagcata atggtacaac
    10381 tacaagtgtt ttactcctca tattaacttc ggtcattaga ggccacgatt tgacacattt
    10441 ttactcaaaa caaaatgttt gcatatctct tataatttca aattcaacac acaacaaata
    10501 agagaaaaaa caaataatat taatttgaga atgaacaaaa ggaccatatc attcattaac
    10561 tcttctccat ccatttccat ttcacagttc gatagcgaaa accgaataaa aaacacagta
    10621 aattacaagc acaacaaatg gtacaagaaa aacagttttc ccaatgccat aatactcaaa
    10681 ctcagtagga ttctggtgtg tgcgcaatga aactgatgca ttgaacttga cgaacgttgt
    10741 cgaaaccgat gatacgaacg aaagctctaa gctcagactt tacgcttctt ctttgggtca
    10801 gccctagagt caccacccaa ctgcgacaga tctatccttg tctcatagag ccctgtaata
    10861 gattggtgaa taagggtagc gtccaacact tccttggttg atgtgtagcg ctttctgtcg
    10921 atagtggtat cgaagtactt aaaggctgct ggggctccca gattggtcag tgtgaacagg
    10981 tggatgatgt tttccgcttg ttcccttatc ggcttatctc tgtgcttgtt gtacgcggaa
    11041 agcaccttta attgtagaaa accagtaaaa catggacaat taggaggcat cgattatcta
    11101 accaaagact ttaacaacac taatctgcca ctaatattca tgagaagtcc ttaccttatc
    11161 gaggttagcg tctgcaagaa tgacccgctt ggaaaattcg ctgatctgct ctatgatctc
    11221 atcaagatag tgcttgtgct gctcgacaaa caactgcttc tgctcgttgt cctctggtga
    11281 ccccttcagc ttctcatagt gtgaggccaa gtacaggaag ttgacgtact tactgggcaa
    11341 tgccagctcg ttgcccttct gcagttctcc ggcagatgcc agcatgcgtt ttctgccatt
    11401 ttccaattcg aaaagcgaat acttgggcag tttgataatg aggtctttct tcacctcttt
    11461 gtaacctggt ttgtataatt ttggttcagt taattaggaa aattacataa tggctccaaa
    11521 aaaaaattat cgacctttta ccaaaaaaaa taaaatttat cgaccttgac aggaaaaagt
    11581 tacaaatcaa aagaaagaat atcgtctggc atccaatatt ttacctttag cctcgaggaa
    11641 gtcgatcgga ttcttctcga acgaggacct ttccattatg gtaattccaa gcagttcctt
    11701 gacgcttttc agtttcttgc tcttgccctt ctccaccttg gccactacca gtacgctgta
    11761 tgccacggta gggctatcaa agcctccgta tttcttcgga tcccaatcct tcttcctcgc
    11821 aatcagtttg tcggagttgc gcttagggag aatggactcc ttggagaaac caccggtttg
    11881 aacctccgtt ttcttgacta tgttgacttg gggcatgctg agaaccttac gcacagtcgc
    11941 aaaatccctg cccttatccc agacaatctc acctgcaaga aacagcaaag agagagttaa
    12001 gatataagtg aacaagaaag tcttgggatc gacatacata caatcaattg atcaaattcc
    12061 taggattaaa catatgaaat aggaaagaca gacctgtttc gccatttgtc tcgatcagag
    12121 gcctcttccg gatttcacca ttcgccagag tgatctctgt cttgaagaag ttcatgatat
    12181 tcgagtaaaa gaagtatttc gcggtagcct tgccgatctc ttgttctgac ttcgcgatca
    12241 tcttcctaac gtcgtacacc ttgtaatccc cgtacacaaa ctcagactcc agtttcggat
    12301 acttcttgat gagagcagtg ccaacaacgg cgttcaggta ggcgtcatgg gcatgatggt
    12361 agttgttgat ctcgcgaacc ttgtaaaact ggaagtcctt ccggaagtca ctaaccagtt
    12421 tgctcttgag ggtgatcacc tgcgtaacca tcaaaagtca atatggattc atcacaaatt
    12481 aatcaggaat agaaagatcg ggtggccttg aagttgatca aataaaaagt ttcgaacttt
    12541 agtttgatat ggtaaaggaa agttggaaac tttaccttta cctccctgat aagtttgtca
    12601 ttctcatcat acttcgtatt catacgcgaa tcgagtatct gggccacatg tttcgtaatc
    12661 tgacgagttt caaccaactg gcgcttgatg aagccggctt tgtcgagctc agacagtccg
    12721 cccctttctg ccttcgtgag attgtcgaat ttccgttggg ttatgagctt agcattcaaa
    12781 agctgcctcc aatagttctt catcttcttc acaacctcct cgctgggtac attgtcggat
    12841 tttccgcggt tcttgtctga gcgcgtaagg acctataaca cgggagaaac caaaaagcaa
    12901 actcatttca cgccaaatac tacagatttc tcaaaaatta agttataatc catatatcct
    12961 gacaagaaat tgattaaaac acagttgctt taccttgttg tcaatactat cgtccttcag
    13021 gaaagattga gggacaatgt gatccacatc atagtcggag agtctgttaa tgtcgagctc
    13081 ttggtcgacg tacatatctc tcccattctg gaggtaatag aggtacagct tctcgttctg
    13141 gagttgagta ttttcgactg ggtgctcttt cagaatctga ctcccaagct ccttgatgcc
    13201 ttcctctatc cgcttcatgc gctcccgtga gttcttctgc cctttctgag tcgtctggtt
    13261 ttccctcgcc atctcgatga cgatgttctc cggcttatgg cggcccatga cctgtagcaa
    13321 taattaaaac aaaaatacca caaacagtta gaactgaggc aagaagaatg aatgcagaga
    13381 aaaaaaatct tagctattca tggaaatgga agtgcgacga acacctaaaa tgcaaggagc
    13441 ataaccaaat gcagaactta ccttcaccag ctcatccaca accttcactg tctgcagtat
    13501 gcccttcttt atagctggcg atccagccag gttggctatg tgttcgtgaa ggctatcgcc
    13561 ctgacctgac acttgggcct tctggatatc ttctttgaag gtcagtgagt catcgtgaat
    13621 aagctgcatg aagttccgat tcgcaaaacc gtcggatttg agaaagtcca gtatagtctt
    13681 gccggattgc ttgtcgcgga tgccgttgat gagcttcctg gagagccttc cccaacctaa
    13741 aacatagaac aaacatttgt ctcatcacaa atatgcatca aggaacactc agaagttagt
    13801 catataccca aattcttgaa actagagaaa caaaaaaaga gggatcattg cggaaacctt
    13861 ttgacctctt acctgtatat ctcctcctct tcaactgctt catgaccttg tcatcgaaca
    13921 gatgcgcata agtcttaagg cgctcctcga tcatttctct gtcctcaaac agcgtgagag
    13981 tcagcacgat gtcctcgagg atgtcctcgt tctcctcgtt gtcaaggaag tccttgtcct
    14041 tgatgatttt caacaggtca tggtatgtgc caagagaggc attgaaccta tcctcaacgc
    14101 cgctgatttc cacagaatca aagcattcga tcttcttaaa gtagtcctct ttaagctgtt
    14161 tcacagtgac cttccgattg gttttgaaca agagatcgac aatggccttc ttctgctcac
    14221 ctgttataac agtaaaaatt actttagaaa caaaacaaaa gtgtggtctt ttaggaatga
    14281 ttattgatca gttcatagtt taaaatcttg tgtttttatc gtaaaagtgt ggaccaggat
    14341 ttacctgaca ggaaggccgg tttgcgcatc ccctccgtca catatttcac cttcgtcagc
    14401 tcgttgtaga ccgtgaagta ttcgtacagg agactatgct tgggcaggac tttctcattt
    14461 gggagattct tatcgaagtt ggtcatcctt tcgatgaagg actgtgcact cgcacccttg
    14521 tccacgacct cttcgaagtt ccaaggcgtg atagtctcct cggatttgcg tgtcatccac
    14581 gcaaaccgcg agttcccgcg tgcgagtgga cctaatgaat taatagaaaa atcaggcaaa
    14641 ttagaaaaga aataattctc aaaagagaac aactgaggtc agaaaatagc attcatcaac
    14701 tgaaactagg tatttcttca tatgatacta acctacatag tagggaatgc ggaacgtgag
    14761 gatcttttca atcttctctc tgttgtcttt caggaacgga tagaagtcct cttgcctcct
    14821 caagatagca tgcagctcac cgaggtggat ctggtgcggt atgctgccat tgtcgaaggt
    14881 acgctgcttc cgaagcaaat cttcgcggtt caatttaacg agcagctcct ctgtaccgtc
    14941 catcttttcc agaataggct tgatgaattt gtagaattcc tcctgacttg cacctaataa
    15001 tatcatcaga ggaaaactca attcttatat cttacagaaa tgaacaacaa aatacatgta
    15061 gcagagagga aaacaatcat aagtaacaac ttacctccat caatgtaccc tgcgtagcca
    15121 ttcttcgatt ggtcaaagaa aatctccttg tacttctcgg ggagttgctg gcgaaccaaa
    15181 gccttcagaa gggtcagatc ctggtgatgc tcgtcgtacc tcttgatcat ggaggcgcta
    15241 agtggcgctt tggttatctc cgtgttgacc cgcagaatgt cactgagcaa gatagcgtct
    15301 gacagattct tggcagccaa gaacaggtcg gcatactgat cacctacaaa agtttaaaag
    15361 actcaatttt aactctacag cttcaaataa atttccgcaa agaaattgat aggtttagac
    15421 aacaaaagaa gagttccaat ttcaagagca cctatttggg cgagcagatt gtccagatca
    15481 tcatcgtatg tatcctttga cagctgcagc ttcgcgtcct cggcgaggtc aaagttgctt
    15541 ttgaagttag gggtgaggcc cagagacagt gcaatcagat tcccgaacaa gccgttcttc
    15601 ttctcgcctg gcaattgagc gatcagattc tcaagccgtc tggacttcga aagtctggca
    15661 gacagtattg ccttggcgtc gacacctgag attgtttgca aagaaaaatc tcaaagcatg
    15721 tacaatctat aaacttatga tggaaaaaga aaagaaaggt aactaaggaa tgttacctga
    15781 tgcattgatg gggttctcct cgaacagctg attgtaggtt tggacgagct gaatgaacag
    15841 tttatccaca tcggagttat ccgggttgag gtccccttcg atcaagaaat ggccacgaaa
    15901 ctttatcatg tgcgcgagag ccaggtatat gagccgaagg tctgccttat cggttgagtc
    15961 caccagcttc ttgcgaaggt ggtatatagt cggatacttt tcatgatagg ctacttcgtc
    16021 aacaatattc ccaaagatgg ggtgacgctc gtgtttctta tcttcctcta ccaggaagga
    16081 ttcctcgagg cgatggaaga acgagtcgtc aacctgcaaa atcaagttta ttgatcatca
    16141 agtgagttta catgtagtta aatgctttca gtaatatccc aatgaagcag gatatgcttt
    16201 cagtacaaaa tacataagca tactatatca taaaaatcct taccttcgcc atctcgttgc
    16261 taaagatctc ttggagataa cagattctgt tcttcctgcg agtgtacctt ctcctcgcag
    16321 tacgtttaag ccttgtcgcc tccgcggttt ctccagagtc aaagagcaga gctccgatca
    16381 agttcttctt aatgctgtgc ctatctgtgt tgcccagcac tttaaatttc ttcgaaggta
    16441 ccttgtattc atcggtgata acggcccacc caactgagtt cgtaccaata tcaagtccaa
    16501 tgctgtactt cttgtccatt ttccagctga ccttccgctt cttcttcggt gggctagaag
    16561 ccattgctgt ggtgagagaa acagagcgtg agctcaaata cacaagtacg aaatcagagt
    16621 tatgagagag gatttggaga aagaagatca cctgaagatc gtttgcgtcg cgcgaatgag
    16681 agagacggca gagaaaacga gaagctttaa gaggacgaac aaaaacccta atgtcactcc
    16741 ttttatatag atgagaaatt tagggtttgg gctctaatgg agctgtctac atgggctttc
    16801 atttctttat ccagtaaaga accggtttaa caggttccct ggtttatacg tggttttagc
    16861 cggttaactt acggaatatt atttttatct taagttacac aaattacatc attaatagta
    16921 aaacaaaatt ttatggtgca atttaagttt ttatgatgag tgaaattcga ataatataca
    16981 aatattttac tgtttaggtt tccctaaacc tacttttaga tatgtaaatt aaacattttt
    17041 ttttttttgg ttagaaccaa gtcagtgatc atagaactca aaaagctggt gtttcaattg
    17101 gtacaagcat ttttattgca agatataaaa attcagaagg acatatcctt gatgtatgta
    17161 catgaaggaa ccgattttga ctccaagcta caaagaagaa gaagaatgat atacaaagaa
    17221 aactgcatat cctcaccatg aaattgaaag ctagaggaag gggaataaca gatactctgc
    17281 gtagaagaga ttccatataa accgataata tcctgagata gcttcctgca ataatcaaat
    17341 gtttctacac tccgttagat atacactcca ttgatgttcc gtgataacga gtttatgttt
    17401 gtacaaaagt attttacctt ggtgtagttt gctttttcta gtacccatgt ctaccaaaga
    17461 tcaacaacaa agatattaga gattaatgaa tttggcagtg tcaaatgcag aaactaaagc
    17521 atatagttat gaagttcttg tgcattatct caaaagtatg taagagatat aagagtatct
    17581 cataatgctt tacctggaaa attaagcctg aagccaagat cacatgtgca ggaatcatca
    17641 agctacctct aaaaacctgt tcattgttaa aggataagag ggttggtctt ttcaaagctg
    17701 catactgagg aaagtaacaa tatcgcaaca aaaataaatt ggtggcgata gtttatcaag
    17761 cttctacatg cagtaatggt gttcgtgctt taattaaccg aaatagttaa gacggaaata
    17821 gatgaattca cctgaggcat gtagaaagct agtgatatgg ctgaaacata atttactagc
    17881 agaagtccag aaccgaggaa tgcaatgttt ctcactccaa gttttgttgc cagggttgat
    17941 atttggaacc tattcaagat caatcaaagg catgattaac taaacatgct tgaacaaaca
    18001 cataaagaca atatatcaaa cggagctggt taaacattgt ctttgactgt caaatggatt
    18061 gagccaaaga gcggaaagaa aatggttatg cgtgcagtta cttactttcg atctccttca
    18121 acatcaggaa ggtcctttgt aatagcaatg accagtgcaa acagtgtcac aaaagatgtg
    18181 atgaacgcca caggtgcact gcaaatagcg aatcaaaagt tgagctccaa gcttgaattc
    18241 ttgcagagac cgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
    18301 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
    18361 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
    18421 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
    18481 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
    18541 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
    18601 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
    18661 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
    18721 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
    18781 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
    18841 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
    18901 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
    18961 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
    19021 atcgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
    19081 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
    19141 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
    19201 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
    19261 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
    19321 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
    19381 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
    19441 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
    19501 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
    19561 gatatggcca gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc
    19621 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
    19681 cttatacaca gccagtcggt ctcagcttct gcaggcatgc gacgtcgagc ttagcttgag
    19741 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
    19801 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
    19861 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
    19921 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg
    19981 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tgttctgaaa acgacatgtc
    20041 gcacaagtcc taagttacgc gacaggctgc cgccctgccc tt
//
