LOCUS       Exported               21653 bp ds-DNA     circular UNA 07-MAY-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 21653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..21653
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          9885..10988
                     /organism="Arabidopsis thaliana"
                     /chromosome="3"
                     /ecotype="Columbia"
                     /mol_type="genomic DNA"
                     /label=chr 3
                     /db_xref="taxon:3702"
     source          18135..19795
                     /organism="Arabidopsis thaliana"
                     /chromosome="3"
                     /ecotype="Columbia"
                     /mol_type="genomic DNA"
                     /label=chr 3
                     /db_xref="taxon:3702"
     source          19816..21279
                     /label=Invitrogen vector 679
                     /note="/invitrogen=679"
     CDS             77..763
                     /label=Excisionase?
                     /note="Ser-Recombinase superfamily"
     misc_feature    complement(823..1823)
                     /label=STA region
                     /note="/feature_type=Misc."
     CDS             1062..1691
                     /label=ParA
                     /note="Partitioning Protein"
     CDS             2086..3192
                     /label=pVS1 replication protein
     primer_bind     2331..2343
                     /label=JS1933
     primer_bind     3688..3710
                     /label=pGEX_3 primer
                     /note="pGEX_3 primer"
     misc_feature    4107..4186
                     /label=ori pBR322
                     /note="ori pBR322"
     primer_bind     complement(4933..4952)
                     /label=JS1630
     CDS             complement(4954..5964)
                     /label=Spect
     primer_bind     5966..5983
                     /label=JS1629
     misc_feature    6265..6299
                     /label=T-DNA LB
                     /note="T-DNA LB"
     misc_feature    6328..6351
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pICH41780 4 parts (3.318bp
                     circular)Fragment:from BpiI cut at 2847^2848 to BpiI cut at
                     2871^2872,24bpOrientation: reverse complementedPlus strand 
                     5':   nochangePlus strand 3':   no changeMinus strand 5':  
                     nochangeMinus strand 3':  no change"
     primer_bind     complement(6332..6355)
                     /label=SENSE_PRM
                     /note="Sense primer used for creating PCR20788
                     link4eb+pstuf4"
     misc_feature    complement(6332..6351)
                     /label=EL4
                     /note="/vntifkey=21"
     misc_feature    6352..8692
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG635 FAST 1-4f (6.693bp
                     circular)Fragment:from BpiI cut at 4335^4336 to BpiI cut at
                     6676^6677,2.341bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    6356..8676
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=E11_pICSL70008 FAST (4.568bp
                     circular)Fragment:from BsaI cut at 2247^2248 to BsaI cut at
                     -1^1,2.321bpOrientation: reverse complementedPlus strand 
                     5':no changePlus strand 3':   no changeMinus
                     strand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(6360..6797)
                     /label=olesin Terminator (A. thaliana)
     CDS             complement(6802..7497)
                     /codon_start=1
                     /label=TagRFP (Entacmaea quadricolor)
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             complement(join(7498..7663,7904..8256))
                     /codon_start=1
                     /label=Olesin (A. thaliana)
                     /translation="MADTARGTHHDIIGRDQYPMMGRDRDQYQMSGRGSDYSKSRQIAK
                     AATAVTAGGSLLVLSSLTLVGTVIALTVATPLLVIFSPILVPALITVALLITGFLSSGG
                     FGIAAITVFSWIYKYATGEHPQGSDKLDSARMKLGSKAQDLKDRAQYYGQQHTGGEHDR
                     DRTRGGQHTT"
     intron          complement(7664..7903)
                     /label=Intron olesin (A.thaliana)
     5'UTR           complement(8257..8324)
                     /label=5'UTR Olesin (A. thaliana)
     misc_signal     complement(8324)
                     /label=Start of transcription
     promoter        complement(8325..8670)
                     /label=Promoter Olesin (A. thaliana)
     TATA_signal     complement(8355..8361)
                     /label=TATA box
     misc_feature    join(8677..8692,6352..6355)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47761 1-4f (4.968bp circular)Target
                     vector:from BsaI cut at 4935^4936 to BsaI cut at 
                     4339^4340,4.372bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    8693..9876
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG634 pnos:Bar-tnos 1-3f
                     (5.536bpcircular)Fragment: from BpiI cut at 4335^4336 to 
                     BpiI cutat 5519^5520, 1.184bpOrientation: originalPlus 
                     strand 5':no changePlus strand 3':   no changeMinus
                     strand 5':  nochangeMinus strand 3':  no change"
     misc_feature    8697..9860
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=D11_pICSL70005 Bar (3.411bp
                     circular)Fragment:from BsaI cut at 2247^2248 to BsaI cut at
                     -1^1,1.164bpOrientation: reverse complementedPlus strand 
                     5':no changePlus strand 3':   no changeMinus
                     strand 5':  nochangeMinus strand 3':  no change"
     terminator      complement(8701..8999)
                     /label=Nos terminator (Agrobacterium tumefaciens)
     CDS             complement(9000..9551)
                     /codon_start=1
                     /label=Bar (phosphinothricin N-acetyltransferase)
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9594..9860)
                     /label=Nos promoter (Agrobacterium tumefaciens)
     misc_feature    join(9861..9876,8693..8696)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47751 1-3f (4.968bp circular)Target
                     vector:from BsaI cut at 4935^4936 to BsaI cut at 
                     4339^4340,4.372bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    complement(9861..9864)
                     /label=GGAG 4bp overhang
                     /label=nonstandard type:  MIsc._Feature
     misc_feature    9877..19811
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK174 1-2f pRPS5:GFP-Cas9-trbcS
                     (corrected)(14.287bp circular)Fragment: from BpiI cut at 
                     4335^4336to BpiI cut at 14270^14271, 
                     9.935bpOrientation:originalPlus strand 5':   no
                     changePlus strand 3':   nochangeMinus strand 5':  no 
                     changeMinus strand 3':  nochange"
     misc_feature    9881..11620
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK124 rbcsE9+intergenic]_Insulator 1
                     3'U+ter(3.987bp circular)Fragment: from BsaI cut at 429^430
                     toBsaI cut at 2169^2170, 1.740bpOrientation: 
                     reversecomplementedPlus strand 5':   no changePlus strand 
                     3':no changeMinus strand 5':  no changeMinus strand 3': 
                     nochange"
     mRNA            9881..10143
                     /locus_tag="AT3G43220"
                     /product="Phosphoinositide phosphatase family protein"
                     /label=AT3G43220 mRNA
                     /db_xref="TAIR:AT3G43220"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EG514215.1,INSD"
     mRNA            9881..10143
                     /locus_tag="AT3G43220"
                     /product="Phosphoinositide phosphatase family protein"
                     /label=AT3G43220 mRNA
                     /db_xref="TAIR:AT3G43220"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EG514215.1,INSD"
                     /inference="Similar to RNAsequence,mRNA:same 
                     species:AY094477.1,INSD"
     gene            9881..10143
                     /locus_tag="AT3G43220"
                     /label=AT3G43220
                     /db_xref="TAIR:AT3G43220"
     primer_bind     9881..9904
                     /label=JS1805
     misc_feature    complement(9885..10988)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Insulator 1 (1.129bp linear)Fragment: from
                     BpiIcut at 10^11 to BpiI cut at 
                     1114^1115,1.104bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    complement(9885..10988)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK113 [Gene]_Insulator 1 selection 
                     (1.128bplinear)Fragment: from BpiI cut at 10^11 to BpiI cut
                     at1114^1115, 1.104bpOrientation: originalPlus strand 5':no 
                     changePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     mRNA            complement(10838..10984)
                     /gene="TES"
                     /gene_synonym="ARABIDOPSIS NPK1-ACTIVATING KINESIN 2; 
                     ATNACK2; NACK2; NPK1-ACTIVATING KINESIN 2; TES; TETRASPORE"
                     /locus_tag="AT3G43210"
                     /product="ATP binding microtubule motor family protein"
                     /label=TES mRNA
                     /db_xref="TAIR:AT3G43210"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EL150582.1,INSD"
                     /inference="Similar to RNA sequence, mRNA:same 
                     species:AJ495781.1"
     gene            complement(10838..10984)
                     /gene="TES"
                     /gene_synonym="ARABIDOPSIS NPK1-ACTIVATING KINESIN 2; 
                     ATNACK2; NACK2; NPK1-ACTIVATING KINESIN 2; TES; TETRASPORE"
                     /locus_tag="AT3G43210"
                     /label=TES
                     /note="Encodes a kinesin TETRASPORE. Required for
                     cytokinesis in pollen. In mutants, all four microspore 
                     nuclei remain within the same cytoplasm after meiosis."
                     /db_xref="TAIR:AT3G43210"
     primer_bind     complement(10963..10988)
                     /label=JS1804
     misc_feature    complement(10989..11624)
                     /label=E9 ter
                     /note="/vntifkey=21"
     misc_feature    complement(10989..11624)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG416_pAGM28545 selection
                     (660bplinear)Fragment: from BpiI cut at 10^11 to BpiI cut 
                     at646^647, 636bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     terminator      complement(10989..11620)
                     /label=rbcS-E9 Terminator (Pisum sativum)
     misc_feature    11621..17368
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pCK069 [CDS1] Cas9-intronized-NLS
                     [pAGM13741](corrected) (7.817bp circular)Fragment: from 
                     BsaI cut at32^33 to BsaI cut at 5780^5781, 
                     5.748bpOrientation:reverse complementedPlus strand 5':
                     no changePlusstrand 3':   no changeMinus strand 5':  no 
                     changeMinusstrand 3':  no change"
     CDS             complement(join(11625..11897,12007..12317,12477..12824,
                     12946..13291,13428..13724,13846..14163,14314..14588,
                     14725..15072,15197..15463,15585..15845,15947..16195,
                     16304..16537,16629..16965,17096..17371))
                     /codon_start=1
                     /label=zCas9
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV"
     intron          complement(11898..12006)
                     /label=intron atipl3
                     /note="/vntifkey=15"
     intron          complement(12318..12476)
                     /label=intron atbg3
                     /note="/vntifkey=15"
     intron          complement(12825..12945)
                     /label=intron e2f58
                     /note="/vntifkey=15"
     intron          complement(13292..13427)
                     /label=intron atatp11
                     /note="/vntifkey=15"
     intron          complement(13725..13845)
                     /label=Intron Rlk7
                     /note="/vntifkey=15"
     intron          complement(14164..14313)
     intron          complement(14589..14724)
                     /label=intron atatp2
                     /note="/vntifkey=15"
     intron          complement(15073..15196)
                     /label=intron atbg11
                     /note="/vntifkey=15"
     intron          complement(15464..15584)
                     /label=intron chtog11
                     /note="/vntifkey=15"
     intron          complement(15846..15946)
                     /label=intron cdpk3
                     /note="/vntifkey=15"
     intron          complement(16196..16303)
                     /label=intron chtog16 (no BpiI)
                     /note="/vntifkey=15"
     variation       16285
                     /label=G to C (no BpiI)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     intron          complement(16538..16628)
                     /label=intron Gluc4
                     /note="/vntifkey=15"
     intron          complement(16966..17095)
                     /label=intron E2f5-4
                     /note="/vntifkey=15"
     misc_feature    17369..18134
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG1155 NLS(cmyc)-GFP as NT1
                     (3.013bpcircular)Fragment: from BsaI cut at 378^379 to BsaI
                     cutat 1144^1145, 766bpOrientation: reverse 
                     complementedPlusstrand 5':   no changePlus strand 3':
                     no changeMinusstrand 5':  no changeMinus strand 3':  no 
                     change"
     primer_bind     17369..17397
                     /label=JS966
     misc_feature    complement(17373..18138)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG170_pAGM1276 [CCAT-AATG]_mEGFP - PCR -
                     NT1selection (790bp linear)Fragment: from BpiI cut at 
                     10^11to BpiI cut at 776^777, 766bpOrientation: 
                     originalPlusstrand 5':   no changePlus strand 3':   no 
                     changeMinusstrand 5':  no changeMinus strand 3':  no 
                     change"
     exon            complement(17373..18096)
                     /label=GFP
     CDS             complement(17378..18091)
                     /codon_start=1
                     /label=mEGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(18074..18138)
                     /label=JS1933
     CDS             complement(18092..18103)
                     /codon_start=1
                     /label=link
                     /translation="SAAA"
     misc_feature    complement(18097..18138)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pAGM1276 [CCAT-AATG] (2.845bp
                     circular)Targetvector: from BpiI cut at 976^977 to BpiI cut
                     at 378^379,2.247bpOrientation: originalPlus strand
                     5':   nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     CDS             complement(18104..18138)
                     /codon_start=1
                     /label=cmyc-NLS
                     /translation="PWLLLPKELNS"
     misc_feature    18135..19795
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pTEI044_pAGM1251 [Pro+5Uf]_pRPS5a 
                     (3.908bpcircular)Fragment: from BsaI cut at 548^549 to BsaI
                     cutat 2209^2210, 1.661bpOrientation: 
                     reversecomplementedPlus strand 5':   no changePlus strand 
                     3':no changeMinus strand 5':  no changeMinus strand 3': 
                     nochange"
     primer_bind     18135..18158
                     /label=REVERSE
     misc_feature    complement(18139..19799)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At3g11940_RPS5a-RC selection
                     (1.687bplinear)Fragment: from BsaI cut at 10^11 to BsaI cut
                     at1673^1674, 1.663bpOrientation: originalPlus strand 5':no 
                     changePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    complement(18139..19799)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG603_pRPS5a [Pro+5U] selection 
                     (1.684bplinear)Fragment: from BpiI cut at 8^9 to BpiI cut 
                     at1669^1670, 1.661bpOrientation: originalPlus strand
                     5':no changePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     gene            complement(18139..18287)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /label=RPS5A
                     /note="One of two genes encoding the ribosomal protein
                     S5. Mutants have semi-dominant developmental phenotypes. 
                     Most cell-division processes are delayed or disturbed in 
                     the heterozygous mutant, and development is completely 
                     arrested at an early embryonic stage in the homozygous 
                     mutant."
                     /db_xref="TAIR:AT3G11940"
     mRNA            complement(18139..18248)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EL264057.1,INSD"
                     /inference="Similar to RNAsequence,mRNA:same 
                     species:AY091376.1,INSD"
     mRNA            complement(18223..18287)
                     /gene="RPS5A"
                     /gene_synonym="AML1; ARABIDOPSIS MINUTE-LIKE 1; ATRPS5A; 
                     MEC18.11; ribosomal protein 5A; RPS5A"
                     /locus_tag="AT3G11940"
                     /product="40S ribosomal protein S5-2"
                     /label=RPS5A mRNA
                     /db_xref="TAIR:AT3G11940"
                     /inference="Similar to RNAsequence,EST:same 
                     species:CB257800.1,INSD"
                     /inference="Similar to RNAsequence,mRNA:same 
                     species:BX824143.1,INSD"
     gene            complement(18620..19799)
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /label=HST
                     /note="Encodes a protein involved in plastoquinone-9 
                     biosynthesis. The enzyme possesses homogentisate 
                     prenyltransferase activity and was shown to use solanesyl 
                     diphosphate, farnesyl diphosphate and 
                     geranylgeranyldiphosphate as prenyl donors, but not 
                     phytyldiphosphate. This gene At3g11945 derives from a split
                     of At3g11950, publications Tian et al (2007) and Sadre et 
                     al (2006) refer to this gene as At3g11950."
                     /db_xref="TAIR:AT3G11945"
     mRNA            complement(join(18620..18896,18989..19021,19165..19227,
                     19403..19520,19677..19769))
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate solanesyltransferase"
                     /label=HST mRNA
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EL101308.1,INSD"
     CDS             complement(join(18822..18896,18989..19021,19165..19227,
                     19403..19520,19677..19769))
                     /codon_start=1
                     /gene="HST"
                     /gene_synonym="ATHST; homogentisate prenyltransferase; HST;
                     PDS2; PHYTOENE DESATURATION 2"
                     /locus_tag="AT3G11945"
                     /product="homogentisate prenyltransferase"
                     /label=HST
                     /note="homogentisate prenyltransferase (HST); CONTAINS
                     InterPro DOMAIN/s: UbiA prenyltransferase 
                     (InterPro:IPR000537); BEST Arabidopsis thaliana protein 
                     match is: homogentisate phytyltransferase 1 
                     (TAIR:AT2G18950.1); Has 1367 Blast hits to 1364 proteins in
                     371 species: Archae - 252; Bacteria - 598; Metazoa -
                     1; Fungi - 4; Plants - 199; Viruses - 0; Other Eukaryotes -
                     313 (source: NCBI BLink); GO_component: GO:0009941 - 
                     chloroplast envelope [Evidence IDA] [PMID 17569077]; 
                     GO_function: GO:0010355 - homogentisate farnesyltransferase
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010356 - homogentisate geranylgeranyltransferase 
                     activity [Evidence IDA] [PMID 16989822]; GO_function: 
                     GO:0010357 - homogentisate solanesyltransferase activity 
                     [Evidence IDA] [PMID 16989822]; GO_process: GO:0010236 - 
                     plastoquinone biosynthetic process [Evidence IMP] [PMID 
                     16989822]"
                     /db_xref="GI:332641605"
                     /db_xref="TAIR:AT3G11945"
                     /inference="Similar to RNAsequence,EST:same 
                     species:EL101308.1,INSD"
                     /protein_id="AEE75126.1"
                     /translation="MTCGVHHIFCDTVCTGHCYYKGPS*C*RRSKVPNINPGNKTWSEK
                     HCIPRFWTSASKLCFSHITSFLHASGF*R*LDDSCTCDLGFRLNFPDMGTRKSKLHQGS
                     YLRILSVYMESLLRRVSVIPLPL"
     primer_bind     complement(19780..19799)
                     /label=FORWARD
     misc_feature    join(19796..19811,9877..9880)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pICH47742 1-2f (4.968bp circular)Target
                     vector:from BsaI cut at 4935^4936 to BsaI cut at 
                     4339^4340,4.372bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    19812..21275
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pJOG294_ccdB (4.200bp circular)Fragment:
                     fromBpiI cut at 442^443 to BpiI cut at 
                     1906^1907,1.464bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     primer_bind     19812..19831
                     /label=1032
     CDS             19933..20592
                     /codon_start=1
                     /label=Cm(R)
                     /note="/invitrogen=1080000"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             20934..21239
                     /codon_start=1
                     /label=ccdB
                     /note="/invitrogen=1060000"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(21260..21279)
                     /label=1033
     misc_feature    join(21276..21653,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pVM_BGW selection (6.700bp
                     circular)Targetvector: from BsaI cut at 7^8 to BsaI cut
                     at 6689^6690,6.682bpOrientation: originalPlus strand 5': 
                     nochangePlus strand 3':   no changeMinus strand 5': 
                     nochangeMinus strand 3':  no change"
     misc_feature    join(21276..21653,1..6327)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pJOG292_pVM_new_mod consensus
                     (7.303bpcircular)Target vector: from BpiI cut at
                     6925^6926 toBpiI cut at 6327^6328, 6.705bpOrientation: 
                     originalPlusstrand 5':   no changePlus strand 3':   no 
                     changeMinusstrand 5':  no changeMinus strand 3':  no 
                     change"
     misc_feature    complement(21360..21385)
                     /label=T-DNA RB
                     /note="T-DNA RB"
     region          21379..21404
                     /label=RB T-DNA repeat
                     /note="Match: GTAAACCTAAGAGAAAAGAGCGTTTA"
                     /note="Length of match: 26"
                     /note="Accuracy (%): 100"
                     /note="Description: pCAMBIA"
                     /note="Found at strand: Positive"
     primer_bind     complement(21383..21402)
                     /label=JS1132
     primer_bind     complement(21575..21596)
                     /label=697
     primer_bind     21594..21613
                     /label=696
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgagcga
     6361 ctaaatggag caacctactg tttttgaagt ttgggttgcc atgtaatcac ttgcaataaa
     6421 tatatgtact aatgactgca tcggcttgcg tgtctaaatt tgtagcttat tgaaaacttt
     6481 ttgtggttaa agacgaatcc agttaactaa cataagttta acaaaacaag accttttcca
     6541 tatatagacc accgttacca acttacaaag aagccaaata aagtacaaca caacacttgc
     6601 aagttacaaa aagagaggaa aaaaaacatg gtaccatttg acaacatata gaaacacaca
     6661 taaacattac ttataacaaa aagattcaaa ctcggctttt attatctccc cctgatcaaa
     6721 ttctaaacac tttattcctc ataaactaac tccccgacat tggagttatt ggactatgac
     6781 gatgacatca gtggggtaag ctcacttgtg ccccagtttg ctagggaggt cgcagtatct
     6841 ggccacagcc acctcgtgct gctcgacgta ggtttctttg tcggcctcct tgattctttc
     6901 cagtctgtgg tccacatagt agacgccggg catcttgagg ttcttagcgg gtttcttgga
     6961 tctgtatgtg gtcttgaagt tgcagatcag gtggcccccg cccacgagct tcagggccat
     7021 gtcgcttctg ccttccaggc cgccgtcagc ggggtacagc atctcggtgt tggcctccca
     7081 gccgagtgtt ttcttctgca tcacagggcc gttggatggg aagttcaccc ctctgatctt
     7141 gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc
     7201 cccatcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttaaa
     7261 gaagtcgggg atgccctggg tgtggttgat gaaggttctg ctgccgtaca tgaagctggt
     7321 agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt
     7381 ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt ggtggttgtt
     7441 cacggtgccc tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctcatagt
     7501 agtgtgctgg ccaccacgag tacggtcacg gtcatgttcc ccaccagtat gttgctgtcc
     7561 gtagtactga gctctgtctt tcagatcctg agctttgctt cccaacttca tccttgcact
     7621 gtccaacttg tctgatccct gtgggtgctc tcccgttgcg tacctattca caatcaatag
     7681 gtatcatacc aagcatttta cctatataga tgatatacac agacacattc aagctagcta
     7741 ggtcaaattt cgtatttagt taaccaaatg ttccctagaa tttgcaattt cttattgtta
     7801 catttgttat tcgaccaaaa aaattgatcc aaagctactg gctcaacaca tgcatgcaca
     7861 tattgcacaa aattatgaag taagatgata aatgtgtgct tacttgtaaa tccaagagaa
     7921 aacggttata gcggcaatgc caaaccctcc agaggaaaga aaaccggtga tgaggagtgc
     7981 aactgtgatg agagccggga caaggattgg gctgaagata acgagcagag gtgttgcaac
     8041 agtcaaagct atgacagttc caacaagggt aaggctggag agaacaagga gggaaccacc
     8101 agctgtgaca gcagttgcag ctttagcaat ctgcctagac ttggagtagt cagatcctcg
     8161 tccggacatc tggtactggt cacgatctcg gcccatcatc gggtactgat ctctgccgat
     8221 gatatcgtga tgggttcctc tagctgtatc cgccattttt ttgttcttgt ttactagaga
     8281 gaatgagatt ttgaatgaga gattgatcaa aaagatgtgt atatattttg gaagaggtag
     8341 gagtgtttgt tagatatatt aagaaaggag acgagaagtc acgtgtcaat ggagacatca
     8401 tgcaattgta ggccacgtgt taatggcgtc accatgcagc cgctgcatgt gttaacgaga
     8461 ggagtctatt caaagacacg tttgtttttt gcctagctca gaatgtggcg ttggcatcga
     8521 accgaaacat aaaacgcaaa acgaaccttt gggctcgtca gtatgggttt gggccttcaa
     8581 tagattcctt ctatacggcc cacatatcca ctattggcaa caaaattaga aaaatttagg
     8641 atttagtttt aaaatttgtt ccgcgacatt ctagaactcc ccatgggaat tcgtaaagcg
     8701 ctccaattca gcaagcgtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     8761 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     8821 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     8881 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     8941 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctagatcca gagtcccgct
     9001 caaatctcag tcacaggaag aacaggtcta ggaggaacag gaagagagaa atcaagctgc
     9061 cagaatccca catcatgcca atttccatgt ttgaaaccag cagctctaag cattccccta
     9121 ggagcataac caagagcctc atgcatcctc acagaaggat cattaggaag tccaatcaca
     9181 gcaaccacag acttgaaacc ctgagcctcc aaagacttca aaagatgagt gtaaagagta
     9241 gatccaagac cagttctttg atgcctagga gacacgtaaa cagtagactc agcagtccaa
     9301 tcgtaagcat tcctagcctt ccaaggacca gcataagcaa taccagccac ttcaccatcc
     9361 acttcagcaa caagccaagg atacctctct ctaagcctca caagatcatc agtccactct
     9421 tgtggttctt gaggctcagt cctgaaattc acagtagaag tctcaatgta atgattcaca
     9481 atagtgcaca cagcaggcat atcagcctca gtagcccttc taatatcagc aggccttctt
     9541 tcaggagaca tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat
     9601 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat tttgacaaga
     9661 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     9721 cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg
     9781 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     9841 gtgactccct taattctccg ctccgagctc gaattctagt agcgtcccaa aatcttagga
     9901 acacggaatg gtacaaaaga gtagtacatt attgctgaat agctgaaacc tttgtgttgc
     9961 ataaaatttc atcattcgtt tgaaaccttt atttttcccc ctaatttgtt ttgtaaatag
    10021 tgtaatgttt atataaagct tgtaagaatc atgttaaaat tatttgtagg tttctttgtc
    10081 tctctgcaat cgtttgaatc acaaacacct gcggttatat attgcgtttg cgttttctga
    10141 agtagtagtc atgagtgact tacttgatac acaattttaa aatgctatta cttgatacca
    10201 aattactggc gcgttttata tacgggaatg gcaaattttg tgttgctata ccgataactg
    10261 acgtgacgtg gcccgttatt agtcctggac attcggtttc agttcgcttc cattcggtta
    10321 tcagtttttc tggttctaaa gcttagtatc cattcggtta ttttgaaagt ttagttcggt
    10381 tctagttttt ttggttttaa aattttgtat ccattcggtt gtttagattg aatatttttg
    10441 gatattttgg ttatttttga atattttcgg tcattttgga tatttttcgg tcatttttgg
    10501 atatttttgg atatttcggt tattttcgag tatttttact attatatata tatttttaat
    10561 tatttagtct agaaataact aatattttaa aaatatattt atatttcgga aagtttcgtt
    10621 tacccgaaat atttcgctac gtatctattt ggtttcagtt ctttcgatta taaaattcag
    10681 tatccattcg gttatttcca actttcggta cgattcggtt tagttatttt tgttttggtt
    10741 tcgattctgt acagtcggtt cagtttattc gcccagacct acccgttata tcgagtaata
    10801 tagaaacatt taccaattta aagaatcaac agagattaga tgatcatatg gttaggattt
    10861 taaaaagagg gttttcacag gtcgtgtgaa tcacactctc tattcagcat caaaagagtt
    10921 ttgaagttaa cgatttacaa acagagaaaa gtagacgtgt aggatttata ggagggtaca
    10981 acagcgttgt caatcaattg gcaagtcata aaatgcatta aaaaatattt tcatactcaa
    11041 ctacaaatcc atgagtataa ctataattat aaagcaatga ttagaatctg acaaggattc
    11101 tggaaaatta cataaaggaa agttcataaa tgtctaaaac acaagaggac atacttgtat
    11161 tcagtaacat ttgcagcttt tctaggtctg aaaatatatt tgttgcctag tgaataagca
    11221 taatggtaca actacaagtg ttttactcct catattaact tcggtcatta gaggccacga
    11281 tttgacacat ttttactcaa aacaaaatgt ttgcatatct cttataattt caaattcaac
    11341 acacaacaaa taagagaaaa aacaaataat attaatttga gaatgaacaa aaggaccata
    11401 tcattcatta actcttctcc atccatttcc atttcacagt tcgatagcga aaaccgaata
    11461 aaaaacacag taaattacaa gcacaacaaa tggtacaaga aaaacagttt tcccaatgcc
    11521 ataatactca aactcagtag gattctggtg tgtgcgcaat gaaactgatg cattgaactt
    11581 gacgaacgtt gtcgaaaccg atgatacgaa cgaaagctct aagctcagac tttacgcttc
    11641 ttctttgggt cagccctaga gtcaccaccc aactgcgaca gatctatcct tgtctcatag
    11701 agccctgtaa tagattggtg aataagggta gcgtccaaca cttccttggt tgatgtgtag
    11761 cgctttctgt cgatagtggt atcgaagtac ttaaaggctg ctggggctcc cagattggtc
    11821 agtgtgaaca ggtggatgat gttttccgct tgttccctta tcggcttatc tctgtgcttg
    11881 ttgtacgcgg aaagcacctt taattgtaga aaaccagtaa aacatggaca attaggaggc
    11941 atcgattatc taaccaaaga ctttaacaac actaatctgc cactaatatt catgagaagt
    12001 ccttacctta tcgaggttag cgtctgcaag aatgacccgc ttggaaaatt cgctgatctg
    12061 ctctatgatc tcatcaagat agtgcttgtg ctgctcgaca aacaactgct tctgctcgtt
    12121 gtcctctggt gaccccttca gcttctcata gtgtgaggcc aagtacagga agttgacgta
    12181 cttactgggc aatgccagct cgttgccctt ctgcagttct ccggcagatg ccagcatgcg
    12241 ttttctgcca ttttccaatt cgaaaagcga atacttgggc agtttgataa tgaggtcttt
    12301 cttcacctct ttgtaacctg gtttgtataa ttttggttca gttaattagg aaaattacat
    12361 aatggctcca aaaaaaaatt atcgaccttt taccaaaaaa aataaaattt atcgaccttg
    12421 acaggaaaaa gttacaaatc aaaagaaaga atatcgtctg gcatccaata ttttaccttt
    12481 agcctcgagg aagtcgatcg gattcttctc gaacgaggac ctttccatta tggtaattcc
    12541 aagcagttcc ttgacgcttt tcagtttctt gctcttgccc ttctccacct tggccactac
    12601 cagtacgctg tatgccacgg tagggctatc aaagcctccg tatttcttcg gatcccaatc
    12661 cttcttcctc gcaatcagtt tgtcggagtt gcgcttaggg agaatggact ccttggagaa
    12721 accaccggtt tgaacctccg ttttcttgac tatgttgact tggggcatgc tgagaacctt
    12781 acgcacagtc gcaaaatccc tgcccttatc ccagacaatc tcacctgcaa gaaacagcaa
    12841 agagagagtt aagatataag tgaacaagaa agtcttggga tcgacataca tacaatcaat
    12901 tgatcaaatt cctaggatta aacatatgaa ataggaaaga cagacctgtt tcgccatttg
    12961 tctcgatcag aggcctcttc cggatttcac cattcgccag agtgatctct gtcttgaaga
    13021 agttcatgat attcgagtaa aagaagtatt tcgcggtagc cttgccgatc tcttgttctg
    13081 acttcgcgat catcttccta acgtcgtaca ccttgtaatc cccgtacaca aactcagact
    13141 ccagtttcgg atacttcttg atgagagcag tgccaacaac ggcgttcagg taggcgtcat
    13201 gggcatgatg gtagttgttg atctcgcgaa ccttgtaaaa ctggaagtcc ttccggaagt
    13261 cactaaccag tttgctcttg agggtgatca cctgcgtaac catcaaaagt caatatggat
    13321 tcatcacaaa ttaatcagga atagaaagat cgggtggcct tgaagttgat caaataaaaa
    13381 gtttcgaact ttagtttgat atggtaaagg aaagttggaa actttacctt tacctccctg
    13441 ataagtttgt cattctcatc atacttcgta ttcatacgcg aatcgagtat ctgggccaca
    13501 tgtttcgtaa tctgacgagt ttcaaccaac tggcgcttga tgaagccggc tttgtcgagc
    13561 tcagacagtc cgcccctttc tgccttcgtg agattgtcga atttccgttg ggttatgagc
    13621 ttagcattca aaagctgcct ccaatagttc ttcatcttct tcacaacctc ctcgctgggt
    13681 acattgtcgg attttccgcg gttcttgtct gagcgcgtaa ggacctataa cacgggagaa
    13741 accaaaaagc aaactcattt cacgccaaat actacagatt tctcaaaaat taagttataa
    13801 tccatatatc ctgacaagaa attgattaaa acacagttgc tttaccttgt tgtcaatact
    13861 atcgtccttc aggaaagatt gagggacaat gtgatccaca tcatagtcgg agagtctgtt
    13921 aatgtcgagc tcttggtcga cgtacatatc tctcccattc tggaggtaat agaggtacag
    13981 cttctcgttc tggagttgag tattttcgac tgggtgctct ttcagaatct gactcccaag
    14041 ctccttgatg ccttcctcta tccgcttcat gcgctcccgt gagttcttct gccctttctg
    14101 agtcgtctgg ttttccctcg ccatctcgat gacgatgttc tccggcttat ggcggcccat
    14161 gacctgtagc aataattaaa acaaaaatac cacaaacagt tagaactgag gcaagaagaa
    14221 tgaatgcaga gaaaaaaaat cttagctatt catggaaatg gaagtgcgac gaacacctaa
    14281 aatgcaagga gcataaccaa atgcagaact taccttcacc agctcatcca caaccttcac
    14341 tgtctgcagt atgcccttct ttatagctgg cgatccagcc aggttggcta tgtgttcgtg
    14401 aaggctatcg ccctgacctg acacttgggc cttctggata tcttctttga aggtcagtga
    14461 gtcatcgtga ataagctgca tgaagttccg attcgcaaaa ccgtcggatt tgagaaagtc
    14521 cagtatagtc ttgccggatt gcttgtcgcg gatgccgttg atgagcttcc tggagagcct
    14581 tccccaacct aaaacataga acaaacattt gtctcatcac aaatatgcat caaggaacac
    14641 tcagaagtta gtcatatacc caaattcttg aaactagaga aacaaaaaaa gagggatcat
    14701 tgcggaaacc ttttgacctc ttacctgtat atctcctcct cttcaactgc ttcatgacct
    14761 tgtcatcgaa cagatgcgca taagtcttaa ggcgctcctc gatcatttct ctgtcctcaa
    14821 acagcgtgag agtcagcacg atgtcctcga ggatgtcctc gttctcctcg ttgtcaagga
    14881 agtccttgtc cttgatgatt ttcaacaggt catggtatgt gccaagagag gcattgaacc
    14941 tatcctcaac gccgctgatt tccacagaat caaagcattc gatcttctta aagtagtcct
    15001 ctttaagctg tttcacagtg accttccgat tggttttgaa caagagatcg acaatggcct
    15061 tcttctgctc acctgttata acagtaaaaa ttactttaga aacaaaacaa aagtgtggtc
    15121 ttttaggaat gattattgat cagttcatag tttaaaatct tgtgttttta tcgtaaaagt
    15181 gtggaccagg atttacctga caggaaggcc ggtttgcgca tcccctccgt cacatatttc
    15241 accttcgtca gctcgttgta gaccgtgaag tattcgtaca ggagactatg cttgggcagg
    15301 actttctcat ttgggagatt cttatcgaag ttggtcatcc tttcgatgaa ggactgtgca
    15361 ctcgcaccct tgtccacgac ctcttcgaag ttccaaggcg tgatagtctc ctcggatttg
    15421 cgtgtcatcc acgcaaaccg cgagttcccg cgtgcgagtg gacctaatga attaatagaa
    15481 aaatcaggca aattagaaaa gaaataattc tcaaaagaga acaactgagg tcagaaaata
    15541 gcattcatca actgaaacta ggtatttctt catatgatac taacctacat agtagggaat
    15601 gcggaacgtg aggatctttt caatcttctc tctgttgtct ttcaggaacg gatagaagtc
    15661 ctcttgcctc ctcaagatag catgcagctc accgaggtgg atctggtgcg gtatgctgcc
    15721 attgtcgaag gtacgctgct tccgaagcaa atcttcgcgg ttcaatttaa cgagcagctc
    15781 ctctgtaccg tccatctttt ccagaatagg cttgatgaat ttgtagaatt cctcctgact
    15841 tgcacctaat aatatcatca gaggaaaact caattcttat atcttacaga aatgaacaac
    15901 aaaatacatg tagcagagag gaaaacaatc ataagtaaca acttacctcc atcaatgtac
    15961 cctgcgtagc cattcttcga ttggtcaaag aaaatctcct tgtacttctc ggggagttgc
    16021 tggcgaacca aagccttcag aagggtcaga tcctggtgat gctcgtcgta cctcttgatc
    16081 atggaggcgc taagtggcgc tttggttatc tccgtgttga cccgcagaat gtcactgagc
    16141 aagatagcgt ctgacagatt cttggcagcc aagaacaggt cggcatactg atcacctaca
    16201 aaagtttaaa agactcaatt ttaactctac agcttcaaat aaatttccgc aaagaaattg
    16261 ataggtttag acaacaaaag aagagttcca atttcaagag cacctatttg ggcgagcaga
    16321 ttgtccagat catcatcgta tgtatccttt gacagctgca gcttcgcgtc ctcggcgagg
    16381 tcaaagttgc ttttgaagtt aggggtgagg cccagagaca gtgcaatcag attcccgaac
    16441 aagccgttct tcttctcgcc tggcaattga gcgatcagat tctcaagccg tctggacttc
    16501 gaaagtctgg cagacagtat tgccttggcg tcgacacctg agattgtttg caaagaaaaa
    16561 tctcaaagca tgtacaatct ataaacttat gatggaaaaa gaaaagaaag gtaactaagg
    16621 aatgttacct gatgcattga tggggttctc ctcgaacagc tgattgtagg tttggacgag
    16681 ctgaatgaac agtttatcca catcggagtt atccgggttg aggtcccctt cgatcaagaa
    16741 atggccacga aactttatca tgtgcgcgag agccaggtat atgagccgaa ggtctgcctt
    16801 atcggttgag tccaccagct tcttgcgaag gtggtatata gtcggatact tttcatgata
    16861 ggctacttcg tcaacaatat tcccaaagat ggggtgacgc tcgtgtttct tatcttcctc
    16921 taccaggaag gattcctcga ggcgatggaa gaacgagtcg tcaacctgca aaatcaagtt
    16981 tattgatcat caagtgagtt tacatgtagt taaatgcttt cagtaatatc ccaatgaagc
    17041 aggatatgct ttcagtacaa aatacataag catactatat cataaaaatc cttaccttcg
    17101 ccatctcgtt gctaaagatc tcttggagat aacagattct gttcttcctg cgagtgtacc
    17161 ttctcctcgc agtacgttta agccttgtcg cctccgcggt ttctccagag tcaaagagca
    17221 gagctccgat caagttcttc ttaatgctgt gcctatctgt gttgcccagc actttaaatt
    17281 tcttcgaagg taccttgtat tcatcggtga taacggccca cccaactgag ttcgtaccaa
    17341 tatcaagtcc aatgctgtac ttcttgtcca ttgcagattt gtacagctcg tccatgccga
    17401 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
    17461 ggtctttgct cagcttggac tgagtactca ggtagtggtt gtcgggcagc agcacggggc
    17521 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
    17581 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
    17641 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
    17701 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
    17761 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
    17821 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
    17881 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
    17941 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
    18001 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
    18061 ccccggtgaa cagctcctcg cccttgctca ctgcggcagc tgagtcgagt ttaactcttt
    18121 tggcagcagg agccatggtg tggtgagaga aacagagcgt gagctcaaat acacaagtac
    18181 gaaatcagag ttatgagaga ggatttggag aaagaagatc acctgaagat cgtttgcgtc
    18241 gcgcgaatga gagagacggc agagaaaacg agaagcttta agaggacgaa caaaaaccct
    18301 aatgtcactc cttttatata gatgagaaat ttagggtttg ggctctaatg gagctgtcta
    18361 catgggcttt catttcttta tccagtaaag aaccggttta acaggttccc tggtttatac
    18421 gtggttttag ccggttaact tacggaatat tatttttatc ttaagttaca caaattacat
    18481 cattaatagt aaaacaaaat tttatggtgc aatttaagtt tttatgatga gtgaaattcg
    18541 aataatatac aaatatttta ctgtttaggt ttccctaaac ctacttttag atatgtaaat
    18601 taaacatttt tttttttttg gttagaacca agtcagtgat catagaactc aaaaagctgg
    18661 tgtttcaatt ggtacaagca tttttattgc aagatataaa aattcagaag gacatatcct
    18721 tgatgtatgt acatgaagga accgattttg actccaagct acaaagaaga agaagaatga
    18781 tatacaaaga aaactgcata tcctcaccat gaaattgaaa gctagaggaa ggggaataac
    18841 agatactctg cgtagaagag attccatata aaccgataat atcctgagat agcttcctgc
    18901 aataatcaaa tgtttctaca ctccgttaga tatacactcc attgatgttc cgtgataacg
    18961 agtttatgtt tgtacaaaag tattttacct tggtgtagtt tgctttttct agtacccatg
    19021 tctaccaaag atcaacaaca aagatattag agattaatga atttggcagt gtcaaatgca
    19081 gaaactaaag catatagtta tgaagttctt gtgcattatc tcaaaagtat gtaagagata
    19141 taagagtatc tcataatgct ttacctggaa aattaagcct gaagccaaga tcacatgtgc
    19201 aggaatcatc aagctacctc taaaaacctg ttcattgtta aaggataaga gggttggtct
    19261 tttcaaagct gcatactgag gaaagtaaca atatcgcaac aaaaataaat tggtggcgat
    19321 agtttatcaa gcttctacat gcagtaatgg tgttcgtgct ttaattaacc gaaatagtta
    19381 agacggaaat agatgaattc acctgaggca tgtagaaagc tagtgatatg gctgaaacat
    19441 aatttactag cagaagtcca gaaccgagga atgcaatgtt tctcactcca agttttgttg
    19501 ccagggttga tatttggaac ctattcaaga tcaatcaaag gcatgattaa ctaaacatgc
    19561 ttgaacaaac acataaagac aatatatcaa acggagctgg ttaaacattg tctttgactg
    19621 tcaaatggat tgagccaaag agcggaaaga aaatggttat gcgtgcagtt acttactttc
    19681 gatctccttc aacatcagga aggtcctttg taatagcaat gaccagtgca aacagtgtca
    19741 caaaagatgt gatgaacgcc acaggtgcac tgcaaatagc gaatcaaaag ttgagctcca
    19801 agcttgaatt cttgcagaga ccgagacgtt gatcggcacg taagaggttc caactttcac
    19861 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
    19921 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
    19981 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
    20041 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
    20101 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
    20161 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
    20221 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
    20281 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
    20341 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
    20401 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
    20461 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
    20521 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
    20581 ggcggggcgt aatcgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
    20641 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
    20701 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
    20761 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
    20821 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
    20881 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
    20941 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
    21001 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
    21061 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
    21121 tgatgaccac cgatatggcc agtgtgccgg tttccgttat cggggaagaa gtggctgatc
    21181 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
    21241 tgtcaggctc ccttatacac agccagtcgg tctcagcttc tgcaggcatg cgacgtcgag
    21301 cttagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
    21361 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
    21421 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
    21481 ggttcccctc gggatcaaag tactttaaag tactttaaag tactttaaag tactttgatc
    21541 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtgttctgaa
    21601 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc ctt
//
