LOCUS       Exported                4403 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2125..4403)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2125..2186))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2125..2186))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2124
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE331 insert 
                     (1.694bplinear)Fragment:fullsequenceOrientation: 
                     reversecomplementedPlus strand5':no changePlus strand 3': 
                     nochangeMinus strand 5':nochangeMinus strand 3':  no 
                     change"
     misc_feature    complement(432..2003)
                     /label=guide RNA1
                     /note="/feature_type=guide RNA"
     misc_feature    complement(445..538)
                     /label=sgRNA
                     /note="/feature_type=sgRNA scaffold"
     CDS             complement(576..881)
                     /label=ccdB
     variation       1201..1225
                     /label=end of cat due to frameshift
                     /label=nonstandard type:  mutation
     CDS             complement(1226..1884)
                     /label=CmR
     variation       1226..1233
                     /label=deletion, frameshift?
                     /label=nonstandard type:  mutation
                     /note="cat gene is functional, beacteria are resistant to 
                     Cloramphenicol."
     misc_feature    complement(2004)
                     /label=TSS
                     /note="/feature_type=Transcription Start Site"
     promoter        complement(2005..2093)
                     /label=pU6-26
     TATA_signal     complement(2028..2035)
                     /label=TATA
     primer_bind     complement(2094..2110)
                     /label=M13f
     misc_feature    join(2125..4403,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2182..2198)
                     /label=M13r
     protein_bind    complement(2204..2224)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2269..2288)
                     /label=pUC_F_JS768
     protein_bind    complement(2271..2308)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2569..3183
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2583)
                     /direction=LEFT
     CDS             complement(3343..4203)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3343..4203)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3346..4134)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4135..4203)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga taaggtctct aagcaaaaaa aagcaccgac tcggtgccac tttttcaagt
      481 tgataacgga ctagccttat ttcaacttgc tatgctgttt ccagcatagc tctgaaacaa
      541 gtcttcgact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
      601 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
      661 gaaaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
      721 accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg
      781 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
      841 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
      901 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
      961 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
     1021 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
     1081 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
     1141 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt
     1201 ttagtaagcc ggatccacgc ggcgtttacg ccccccctgc cactcatcgc agtactgttg
     1261 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1321 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1381 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1441 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1501 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1561 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1621 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     1681 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1741 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1801 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     1861 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1921 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1981 ccgatcaacg tctcagaaga cttcaatcac tacttcgact ctagctgtat ataaactcag
     2041 cttcgttttc ttatctaagc gatgtgggac ttttgaagat tgttttcaac ttagtcgtga
     2101 ctgggaaaac gcaatgagac caaaatcgga tcccgggccc gtcgactgca gaggcctgca
     2161 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2221 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2281 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2341 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2401 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3001 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3481 gcgagagcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4321 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4381 gcgtatcacg aggccctttc gtc
//
