LOCUS       Exported                4402 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2124..4402)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2124..2185))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2124..2185))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2123
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE332 M1_improved 
                     (1.694bplinear)Fragment:fullsequenceOrientation: 
                     reversecomplementedPlus strand5':no changePlus strand 3':no
                     changeMinus strand5':  nochangeMinus strand 3': nochange"
     misc_feature    complement(432..2004)
                     /label=guide RNA1
                     /note="/feature_type=guide RNA"
     misc_feature    complement(446..539)
                     /label=sgRNA
                     /note="/feature_type=sgRNA scaffold"
     CDS             complement(577..882)
                     /label=ccdB
     variation       1202..1226
                     /label=end of cat due to frameshift
                     /label=nonstandard type:  mutation
     CDS             complement(1227..1885)
                     /label=CmR
     variation       1227..1234
                     /label=deletion, frameshift?
                     /label=nonstandard type:  mutation
                     /note="cat gene is functional, beacteria are resistant to 
                     Cloramphenicol."
     misc_feature    complement(2005)
                     /label=TSS
                     /note="/feature_type=Transcription Start Site"
     promoter        complement(2006..2094)
                     /label=pU6-26
     TATA_signal     complement(2029..2036)
                     /label=TATA
     primer_bind     complement(2095..2111)
                     /label=M13f
     misc_feature    join(2124..4402,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2181..2197)
                     /label=M13r
     protein_bind    complement(2203..2223)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2268..2287)
                     /label=pUC_F_JS768
     protein_bind    complement(2270..2307)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2568..3182
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2582)
                     /direction=LEFT
     CDS             complement(3342..4202)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3342..4202)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3345..4133)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4134..4202)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttttggtctc aagggaaaaa aaagcaccga ctcggtgcca ctttttcaag
      481 ttgataacgg actagcctta tttcaacttg ctatgctgtt tccagcatag ctctgaaaca
      541 agtcttcgac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
      601 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      661 ggaaaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      721 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
      781 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
      841 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
      901 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
      961 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     1021 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     1081 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     1141 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     1201 tttagtaagc cggatccacg cggcgtttac gccccccctg ccactcatcg cagtactgtt
     1261 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1321 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1381 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1441 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1501 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1561 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1621 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     1681 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1741 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1801 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1861 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1921 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1981 gccgatcaac gtctcagaag acttcaatca ctacttcgac tctagctgta tataaactca
     2041 gcttcgtttt cttatctaag cgatgtggga cttttgaaga ttgttttcaa cttagtcgtg
     2101 actgggaaaa cgcaatgaga ccaatcggat cccgggcccg tcgactgcag aggcctgcat
     2161 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3001 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3481 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4321 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4381 cgtatcacga ggccctttcg tc
//
