LOCUS       Exported                4385 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 6, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2107..4385)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2107..2168))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2107..2168))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2106
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE332 M1_improved 
                     (1.694bplinear)Fragment:fullsequenceOrientation: 
                     originalPlusstrand 5':nochangePlus strand 3':   no 
                     changeMinusstrand 5':nochangeMinus strand 3':  no change"
     misc_feature    complement(432..2106)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE338
                     M5_improvedinsert(1.698bplinear)Fragment: 
                     fullsequenceOrientation:reversecomplementedPlus strand 
                     5':no changePlus strand3':no changeMinus strand 5': 
                     nochangeMinus strand 3':nochange"
     misc_feature    432..2106
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=PCR AtU6 M5 new-RC 
                     (1.676bplinear)Fragment:fullsequenceOrientation: 
                     originalPlusstrand 5':nochangePlus strand 3':   no 
                     changeMinus strand5':nochangeMinus strand 3':  no change"
     primer_bind     432..462
                     /label=JS1885
     promoter        444..532
                     /label=pU6-26
     TATA_signal     502..509
                     /label=TATA
     misc_feature    533
                     /label=TSS
                     /note="/feature_type=Transcription Start Site"
     misc_feature    534..2106
                     /label=guide RNA1
                     /note="/feature_type=guide RNA"
     CDS             653..1311
                     /label=CmR
     variation       1304..1311
                     /label=deletion, frameshift?
                     /label=nonstandard type:  mutation
                     /note="cat gene is functional, beacteria are resistant to 
                     Cloramphenicol."
     variation       1312..1336
                     /label=end of cat due to frameshift
                     /label=nonstandard type:  mutation
     CDS             1656..1961
                     /label=ccdB
     misc_feature    1999..2092
                     /label=sgRNA
                     /note="/feature_type=sgRNA scaffold"
     primer_bind     complement(2075..2106)
                     /label=JS741
     misc_feature    join(2107..4385,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2164..2180)
                     /label=M13r
     protein_bind    complement(2186..2206)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2251..2270)
                     /label=pUC_F_JS768
     protein_bind    complement(2253..2290)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2551..3165
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2565)
                     /direction=LEFT
     CDS             complement(3325..4185)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3325..4185)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3328..4116)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4117..4185)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttggtctcat gcgtaagttg aaaacaatct tcaaaagtcc cacatcgctt
      481 agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga ttgaagtctt
      541 ctgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
      601 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
      661 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      721 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      781 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      841 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      901 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      961 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1021 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1081 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1141 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1201 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1261 aatgcttaat gaattacaac agtactgcga tgagtggcag ggggggcgta aacgccgcgt
     1321 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     1381 ataagaatat atactgatat gtatacccga agtatgtcag aaagaggtat gctatgaagc
     1441 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     1501 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     1561 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     1621 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     1681 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     1741 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     1801 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     1861 ccagtgtgcc ggtttccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     1921 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     1981 acagccagtc gaagacttgt ttcagagcta tgctggaaac agcatagcaa gttgaaataa
     2041 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ttgcttagag
     2101 acctttatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc
     2161 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2221 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2281 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3001 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3121 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3301 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3361 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3421 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     3481 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3541 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3601 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3661 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3721 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3781 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3841 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3901 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3961 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4021 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4081 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4141 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4201 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4261 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4321 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4381 tcgtc
//
