LOCUS       Exported                4387 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2109..4387)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2109..2170))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2109..2170))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    complement(432..2108)
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE332 M1_improved 
                     (1.694bplinear)Fragment:fullsequenceOrientation: 
                     originalPlusstrand 5':nochangePlus strand 3':   no 
                     changeMinusstrand 5':nochangeMinus strand 3':  no change"
     misc_feature    432..2108
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE341
                     M7_improvedinsert(1.677bplinear)Fragment: 
                     fullsequenceOrientation:reversecomplementedPlus strand 
                     5':no changePlus strand3':no changeMinus strand 5': 
                     nochangeMinus strand 3':nochange"
     misc_feature    complement(432..2004)
                     /label=guide RNA1
                     /note="/feature_type=guide RNA"
     misc_feature    complement(446..539)
                     /label=sgRNA
                     /note="/feature_type=sgRNA scaffold"
     CDS             complement(577..882)
                     /label=ccdB
     variation       1202..1226
                     /label=end of cat due to frameshift
                     /label=nonstandard type:  mutation
     CDS             complement(1227..1885)
                     /label=CmR
     variation       1227..1234
                     /label=deletion, frameshift?
                     /label=nonstandard type:  mutation
                     /note="cat gene is functional, beacteria are resistant to 
                     Cloramphenicol."
     misc_feature    complement(2005)
                     /label=TSS
                     /note="/feature_type=Transcription Start Site"
     promoter        complement(2006..2094)
                     /label=pU6-26
     TATA_signal     complement(2029..2036)
                     /label=TATA
     misc_feature    join(2109..4387,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2166..2182)
                     /label=M13r
     protein_bind    complement(2188..2208)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2253..2272)
                     /label=pUC_F_JS768
     protein_bind    complement(2255..2292)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2553..3167
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2567)
                     /direction=LEFT
     CDS             complement(3327..4187)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3327..4187)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3330..4118)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4119..4187)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttttggtctc attcgaaaaa aaagcaccga ctcggtgcca ctttttcaag
      481 ttgataacgg actagcctta tttcaacttg ctatgctgtt tccagcatag ctctgaaaca
      541 agtcttcgac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
      601 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      661 ggaaaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      721 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
      781 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
      841 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
      901 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
      961 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     1021 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     1081 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     1141 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     1201 tttagtaagc cggatccacg cggcgtttac gccccccctg ccactcatcg cagtactgtt
     1261 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1321 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1381 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1441 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1501 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1561 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1621 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     1681 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1741 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1801 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1861 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1921 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1981 gccgatcaac gtctcagaag acttcaatca ctacttcgac tctagctgta tataaactca
     2041 gcttcgtttt cttatctaag cgatgtggga cttttgaaga ttgttttcaa cttactggtg
     2101 agaccaaaat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
     2161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca
     3481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4321 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4381 tttcgtc
//
