LOCUS       Exported                4457 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2179..4457)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2179..2240))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2179..2240))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2178
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE412-SlU6-M1Eselection(1.749bplinear)Frag
                     ment: fullsequenceOrientation:originalPlusstrand 5': 
                     nochangePlus strand 3':   nochangeMinusstrand 5': 
                     nochangeMinus strand 3':  nochange"
     primer_bind     432..481
                     /label=JS1829
     primer_bind     444..460
                     /label=M13f
     promoter        461..608
                     /label=SlU6 promoter fragment
     misc_feature    577..584
                     /label=TATA box
                     /label=nonstandard type:  TATA
     misc_feature    605
                     /label=Mutation to adapt overhang
                     /label=nonstandard type:  SNP
     misc_feature    608
                     /label=TSS
                     /label=nonstandard type:  TSS
     CDS             728..1387
                     /label=CmR
     CDS             1729..2034
                     /label=ccdB
     misc_feature    2072..2165
                     /label=sgRNA scaffold
                     /label=nonstandard type:  sgRNA
     primer_bind     complement(2150..2178)
                     /label=JS741
     misc_feature    join(2179..4457,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2236..2252)
                     /label=M13r
     protein_bind    complement(2258..2278)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2323..2342)
                     /label=pUC_F_JS768
     protein_bind    complement(2325..2362)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2623..3237
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2637)
                     /direction=LEFT
     CDS             complement(3397..4257)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3397..4257)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3400..4188)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4189..4257)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttggtctcat tgcgttttcc cagtcacgac caagagaaat aagaaaaagg
      481 ccggcccatt tagattgaag atccaggccc aggcccgtaa aagaaaccca acaagcaaat
      541 tctccctcat cgcttataca aagctacttt gcctcgttta tatagcggaa tatgaacatg
      601 tatgattgaa gtcttctgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
      661 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
      721 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      781 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      841 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      901 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
      961 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     1021 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     1081 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     1141 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     1201 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     1261 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
     1321 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     1381 ggcgtaatcg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     1441 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     1501 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     1561 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     1621 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     1681 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     1741 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     1801 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     1861 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     1921 accaccgata tggccagtgt gccggtttcc gttatcgggg aagaagtggc tgatctcagc
     1981 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     2041 ggctccctta tacacagcca gtcgaagact tgtttcagag ctatgctgga aacagcatag
     2101 caagttgaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     2161 tttttgctta gagaccttat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag
     2221 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2281 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2341 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2401 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3541 gccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4381 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4441 cacgaggccc tttcgtc
//
