LOCUS       Exported                4443 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2165..4443)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2165..2226))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2165..2226))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2164
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE412-SlU6-M1Eselection(1.733bplinear)Frag
                     ment: fullsequenceOrientation:originalPlusstrand 5': 
                     nochangePlus strand 3':   nochangeMinusstrand 5': 
                     nochangeMinus strand 3':  nochange"
     primer_bind     432..466
                     /label=JS2052
     promoter        446..593
                     /label=SlU6 promoter fragment
     misc_feature    562..569
                     /label=TATA box
                     /label=nonstandard type:  TATA
     misc_feature    590
                     /label=Mutation to adapt overhang
                     /label=nonstandard type:  SNP
     misc_feature    593
                     /label=TSS
                     /label=nonstandard type:  TSS
     CDS             713..1372
                     /label=CmR
     CDS             1714..2019
                     /label=ccdB
     misc_feature    2057..2164
                     /label=sgRNA scaffold
                     /label=nonstandard type:  sgRNA
     primer_bind     complement(2133..2164)
                     /label=JS786
     misc_feature    join(2165..4443,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2222..2238)
                     /label=M13r
     protein_bind    complement(2244..2264)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2309..2328)
                     /label=pUC_F_JS768
     protein_bind    complement(2311..2348)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2609..3223
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2623)
                     /direction=LEFT
     CDS             complement(3383..4243)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3383..4243)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3386..4174)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4175..4243)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttttggtctc accctcaaga gaaataagaa aaaggccggc ccatttagat
      481 tgaagatcca ggcccaggcc cgtaaaagaa acccaacaag caaattctcc ctcatcgctt
      541 atacaaagct actttgcctc gtttatatag cggaatatga acatgtatga ttgaagtctt
      601 ctgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
      661 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
      721 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      781 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      841 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      901 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      961 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1021 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1081 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1141 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1201 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1261 gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag
     1321 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatcgcgtgg
     1381 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     1441 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     1501 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     1561 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     1621 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     1681 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     1741 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     1801 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1861 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1921 agtgtgccgg tttccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1981 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     2041 agccagtcga agacttgttt cagagctatg ctggaaacag catagcaagt tgaaataagg
     2101 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttt cggttgagac
     2161 caaaatcgga tcccgggccc gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat
     2221 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2281 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2341 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3061 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg
     3541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4381 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4441 gtc
//
