LOCUS       Exported                4442 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2164..4442)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2164..2225))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2164..2225))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2163
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE412-SlU6-M1Eselection(1.733bplinear)Frag
                     ment: fullsequenceOrientation:originalPlusstrand 5': 
                     nochangePlus strand 3':   nochangeMinusstrand 5': 
                     nochangeMinus strand 3':  nochange"
     primer_bind     432..465
                     /label=JS2053
     promoter        445..592
                     /label=SlU6 promoter fragment
     misc_feature    561..568
                     /label=TATA box
                     /label=nonstandard type:  TATA
     misc_feature    589
                     /label=Mutation to adapt overhang
                     /label=nonstandard type:  SNP
     misc_feature    592
                     /label=TSS
                     /label=nonstandard type:  TSS
     CDS             712..1371
                     /label=CmR
     CDS             1713..2018
                     /label=ccdB
     misc_feature    2056..2163
                     /label=sgRNA scaffold
                     /label=nonstandard type:  sgRNA
     primer_bind     complement(2132..2163)
                     /label=JS788
     misc_feature    join(2164..4442,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2221..2237)
                     /label=M13r
     protein_bind    complement(2243..2263)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2308..2327)
                     /label=pUC_F_JS768
     protein_bind    complement(2310..2347)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2608..3222
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2622)
                     /direction=LEFT
     CDS             complement(3382..4242)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3382..4242)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3385..4173)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4174..4242)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tttggtctca cggtcaagag aaataagaaa aaggccggcc catttagatt
      481 gaagatccag gcccaggccc gtaaaagaaa cccaacaagc aaattctccc tcatcgctta
      541 tacaaagcta ctttgcctcg tttatatagc ggaatatgaa catgtatgat tgaagtcttc
      601 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      661 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
      721 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      781 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      841 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      901 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      961 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1021 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1081 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1141 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1201 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1261 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
     1321 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atcgcgtgga
     1381 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     1441 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     1501 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     1561 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     1621 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     1681 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     1741 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     1801 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     1861 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     1921 gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     1981 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2041 gccagtcgaa gacttgtttc agagctatgc tggaaacagc atagcaagtt gaaataaggc
     2101 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttta tggtgagacc
     2161 aaaatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
     2221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc
     3541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4381 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4441 tc
//
