LOCUS       Exported                4461 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2183..4461)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2183..2244))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2183..2244))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2182
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE412-SlU6-M1Eselection(1.754bplinear)Frag
                     ment: fullsequenceOrientation:originalPlusstrand 5': 
                     nochangePlus strand 3':   nochangeMinusstrand 5': 
                     nochangeMinus strand 3':  nochange"
     primer_bind     432..486
                     /label=JS2055
     primer_bind     445..465
                     /label=JS838
     promoter        466..613
                     /label=SlU6 promoter fragment
     misc_feature    582..589
                     /label=TATA box
                     /label=nonstandard type:  TATA
     misc_feature    610
                     /label=Mutation to adapt overhang
                     /label=nonstandard type:  SNP
     misc_feature    613
                     /label=TSS
                     /label=nonstandard type:  TSS
     CDS             733..1392
                     /label=CmR
     CDS             1734..2039
                     /label=ccdB
     misc_feature    2077..2182
                     /label=sgRNA scaffold
                     /label=nonstandard type:  sgRNA
     primer_bind     complement(2153..2182)
                     /label=JS1884
     misc_feature    join(2183..4461,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2240..2256)
                     /label=M13r
     protein_bind    complement(2262..2282)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2327..2346)
                     /label=pUC_F_JS768
     protein_bind    complement(2329..2366)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2627..3241
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2641)
                     /direction=LEFT
     CDS             complement(3401..4261)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3401..4261)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3404..4192)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4193..4261)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tttggtctca gactgccagc ttctatgagt actgacaaga gaaataagaa
      481 aaaggccggc ccatttagat tgaagatcca ggcccaggcc cgtaaaagaa acccaacaag
      541 caaattctcc ctcatcgctt atacaaagct actttgcctc gtttatatag cggaatatga
      601 acatgtatga ttgaagtctt ctgagacgtt gatcggcacg taagaggttc caactttcac
      661 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      721 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      781 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      841 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      901 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      961 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1021 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1081 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1141 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1201 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1261 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     1321 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1381 ggcggggcgt aatcgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1441 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1501 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1561 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1621 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1681 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1741 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1801 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1861 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1921 tgatgaccac cgatatggcc agtgtgccgg tttccgttat cggggaagaa gtggctgatc
     1981 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     2041 tgtcaggctc ccttatacac agccagtcga agacttgttt cagagctatg ctggaaacag
     2101 catagcaagt tgaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2161 gctttttttt tgcgtgagac caatcggatc ccgggcccgt cgactgcaga ggcctgcatg
     2221 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3541 gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4441 gtatcacgag gccctttcgt c
//
