LOCUS       Exported                4440 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2162..4440)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2162..2223))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2162..2223))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2161
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE412-SlU6-M1Eselection(1.732bplinear)Frag
                     ment: fullsequenceOrientation:originalPlusstrand 5': 
                     nochangePlus strand 3':   nochangeMinusstrand 5': 
                     nochangeMinus strand 3':  nochange"
     primer_bind     432..464
                     /label=JS2058
     promoter        444..591
                     /label=SlU6 promoter fragment
     misc_feature    560..567
                     /label=TATA box
                     /label=nonstandard type:  TATA
     misc_feature    588
                     /label=Mutation to adapt overhang
                     /label=nonstandard type:  SNP
     misc_feature    591
                     /label=TSS
                     /label=nonstandard type:  TSS
     CDS             711..1370
                     /label=CmR
     CDS             1712..2017
                     /label=ccdB
     misc_feature    2055..2161
                     /label=sgRNA scaffold
                     /label=nonstandard type:  sgRNA
     primer_bind     complement(2133..2161)
                     /label=JS741
     misc_feature    join(2162..4440,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI 
                     (2.710bpcircular)Targetvector:plasmid
                     opened by EcoRV cutat431^432Orientation:originalPlus strand
                     5':nochangePlus strand 3':   nochangeMinus strand 
                     5':nochangeMinus strand 3':  nochange"
     primer_bind     complement(2219..2235)
                     /label=M13r
     protein_bind    complement(2241..2261)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2306..2325)
                     /label=pUC_F_JS768
     protein_bind    complement(2308..2345)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2606..3220
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2620)
                     /direction=LEFT
     CDS             complement(3380..4240)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3380..4240)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3383..4171)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4172..4240)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttggtctcac gaacaagaga aataagaaaa aggccggccc atttagattg
      481 aagatccagg cccaggcccg taaaagaaac ccaacaagca aattctccct catcgcttat
      541 acaaagctac tttgcctcgt ttatatagcg gaatatgaac atgtatgatt gaagtcttct
      601 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
      661 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      721 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      781 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      841 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      901 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      961 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     1021 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     1081 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     1141 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     1201 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     1261 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
     1321 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tcgcgtggat
     1381 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
     1441 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg
     1501 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     1561 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     1621 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     1681 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag
     1741 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     1801 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     1861 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     1921 tgtgccggtt tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     1981 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     2041 ccagtcgaag acttgtttca gagctatgct ggaaacagca tagcaagttg aaataaggct
     2101 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttttgc ttagagacct
     2161 tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt
     2221 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2281 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2341 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2401 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3061 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3301 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3361 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3421 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3481 agggcttacc atctggcccc agtgctgcaa tgataccgcg agagccacgc tcaccggctc
     3541 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3601 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3661 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3721 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3781 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3841 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3901 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3961 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4021 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4081 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4141 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4201 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4261 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4321 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     4381 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
