LOCUS       Exported                4449 bp ds-DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; l...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 4449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 6, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..431,2171..4449)
                     /organism="Cloning vector pUC57"
                     /lab_host="E.coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:66198"
     CDS             complement(join(146..431,2171..2232))
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="N-terminal fragment"
                     /db_xref="GI:2440163"
                     /protein_id="CAA75110.1"
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
                     CTLSTICSDAA"
     gene            complement(join(146..431,2171..2232))
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     170..189
                     /label=JS703
     primer_bind     263..280
                     /label=pUC_R_JS767
     primer_bind     359..375
                     /label=M13f
     misc_feature    432..2170
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE413 SlU3-M1E selection (1.765bp
                     linear)Fragment: full sequenceOrientation: originalPlus 
                     strand 5':   no changePlus strand 3':   no changeMinus 
                     strand 5':  no changeMinus strand 3':  no change"
     misc_feature    432..2170
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pDGE493 SlU3 M5new selection (1.743bp
                     linear)Fragment: full sequenceOrientation: originalPlus 
                     strand 5':   no changePlus strand 3':   no changeMinus 
                     strand 5':  no changeMinus strand 3':  no change"
     primer_bind     432..465
                     /label=JS1886
     promoter        444..600
                     /label=SlU3 promoter fragment
     misc_feature    600
                     /label=Transcript
                     /label=nonstandard type:  Transcript
                     /note="/Alias=U3"
                     /note="/ID=Solyc06r072195.1.1"
                     /note="/Name=RF00012"
                     /note="/e-value=1.245e-20"
                     /note="/rna_type=snRNA"
                     /note="/score=133.64"
                     /note="/source=ITAG_infernal"
     CDS             720..1403
                     /label=CmR (variant)
     CDS             1723..2028
                     /label=ccdB
     misc_feature    2066..2159
                     /label=sgRNA scaffold
                     /note="/feature_type=sgRNA scaffold"
     primer_bind     complement(2142..2170)
                     /label=JS796
     misc_feature    join(2171..4449,1..431)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=pUC57-BsaI (2.710bp circular)Target vector:
                     plasmid opened by EcoRV cut at 431^432Orientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     primer_bind     complement(2228..2244)
                     /label=M13r
     protein_bind    complement(2250..2270)
                     /label=lacI binding site
                     /bound_moiety="lacI"
                     /note="lac repressor"
     primer_bind     complement(2315..2334)
                     /label=pUC_F_JS768
     protein_bind    complement(2317..2354)
                     /label=CAP binding site
                     /bound_moiety="CAP"
     misc_feature    2615..3229
                     /label=replicon of pMB1
                     /note="replicon of pMB1"
     rep_origin      complement(2629)
                     /direction=LEFT
     CDS             complement(3389..4249)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers resistance to ampicilin"
                     /label=bla
                     /db_xref="GI:4379389"
                     /protein_id="CAA75111.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3389..4249)
                     /gene="bla"
                     /label=bla
     mat_peptide     complement(3392..4180)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4181..4249)
                     /gene="bla"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttggtctcat gcgcaaaaac ttcaaaatct aacacaaatt tttattgtat
      481 ttgagcagat ttgcataata gtcagcgaag ctgtgtcaag ggccaacata ttattcccac
      541 atcggtaggt caacacataa actccaggtt tatgaggctt cgtagcagta gcacatgtca
      601 aagtcttctg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      661 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      721 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      781 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      841 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      901 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
      961 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1021 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1081 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1141 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1201 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1261 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1321 tcggtagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggg gggcgtaaac
     1381 gccgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1441 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     1501 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1561 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1621 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1681 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     1741 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1801 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1861 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1921 gatatggcca gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc
     1981 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2041 cttatacaca gccagtcgaa gacttgtttc agagctatgc tggaaacagc atagcaagtt
     2101 gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttc
     2161 cagtgagacc atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt
     2221 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2281 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2341 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2401 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2461 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2521 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2581 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2641 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2701 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2761 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2821 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2881 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2941 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3001 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3061 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3301 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3361 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3421 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3481 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gagccacgct
     3541 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3601 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3661 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3721 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3781 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3841 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3901 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3961 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4021 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4081 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4141 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4201 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4261 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4321 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4381 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4441 cctttcgtc
//
