LOCUS       Exported                3502 bp ds-DNA     circular UNA 14-JUN-2013
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          359..1826
                     /label=Invitrogen vector 679
                     /note="/invitrogen=679"
     rep_origin      complement(105)
                     /direction=LEFT
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     primer_bind     258..276
                     /label=JS1691
     primer_bind     complement(331..358)
                     /label=JS1878
     misc_feature    351..354
                     /label=ACAT
                     /note="/vntifkey=21"
     primer_bind     355..388
                     /label=JS1879
     CDS             474..1157
                     /codon_start=1
                     /label=Cm(R) [variant]
                     /note="/invitrogen=1080000"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     CDS             1477..1782
                     /codon_start=1
                     /label=ccdB
                     /note="/invitrogen=1060000"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1794..1826)
                     /label=JS1880
     misc_feature    join(1823..3502,1..354)
                     /label=Vector
                     /label=nonstandard type:  Vector
                     /note="/origin=3 backbone 
                     PCR-1-1(2.057bpcircular)Targetvector:
                     from BsmBI cut at 10^11 toBsmBIcut at2043^2044, 
                     2.033bpOrientation: originalPlusstrand5':no changePlus 
                     strand 3':   no changeMinus strand5':nochangeMinus strand 
                     3':  no change"
     primer_bind     1823..1844
                     /label=JS1877
     misc_feature    1827..1830
                     /label=TTGT
                     /note="/vntifkey=21"
     primer_bind     complement(1909..1928)
                     /label=JS1692
     CDS             2054..2845
                     /codon_start=1
                     /label=NPTII
                     /note="/vntifkey=4"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       2536
                     /label=A to G (BpiI removed)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
ORIGIN
        1 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      301 aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tcctgtgaag acatcggttt
      361 gctgagacct tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
      421 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
      481 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      541 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      601 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      661 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      721 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      781 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      841 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      901 ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      961 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1021 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1081 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggggggcgt aaacgccgcg
     1141 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1201 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctatgaag
     1261 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1321 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1381 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1441 acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt ttacacctat
     1501 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgcct
     1561 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1621 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     1681 gccagtgtgc cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     1741 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     1801 cacagccagt cggtctcagc ttgactttgt cttcacgaag tggctcttca gtggacgaaa
     1861 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2041 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     2101 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     2161 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     2221 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     2281 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     2341 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     2401 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     2461 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     2521 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     2581 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     2641 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     2701 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     2761 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     2821 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
     2881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3121 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3481 aggagagcgc acgagggagc tt
//
