LOCUS       Exported                2045 bp ds-DNA     circular UNA 14-JUN-2013
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      complement(98)
                     /direction=LEFT
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     primer_bind     251..269
                     /label=JS1691
     misc_feature    346..363
                     /label=end linker
                     /note="/feature_type=end linker"
     misc_feature    join(351..2045,1..350)
                     /label=Fragment
                     /label=nonstandard type:  Fragment
                     /note="/origin=ELE2-1 (2.070bp circular)Fragment:
                     fromBsmBIcutat 1830^1831 to BsmBI 
                     cutat1805^1806,2.045bpOrientation: originalPlus 
                     strand5':nochangePlus strand 3':   no changeMinus 
                     strand5':nochangeMinus strand 3':  no change"
     primer_bind     complement(445..464)
                     /label=JS1692
     CDS             590..1381
                     /codon_start=1
                     /label=NPTII
                     /note="/vntifkey=4"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     variation       1072
                     /label=A to G (BpiI removed)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
ORIGIN
        1 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
       61 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      301 ctctccccgc gcgttggccg attcattaat cactctgtgg tctcacggta taagctttag
      361 ctttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt
      421 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat
      601 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
      661 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
      721 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
      781 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
      841 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
      901 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
      961 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     1021 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc
     1081 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     1141 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     1201 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     1261 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     1321 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     1381 ttagctgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1681 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2041 agggg
//
