LOCUS       Exported               13639 bp ds-DNA     circular SYN 07-MAY-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     20..30
                     /label=350_VG-VV_chiRNA_R
                     /note="color: black; sequence: TCTAGAATGCATACGCATTAAGCG"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     propeptide      667..1144
                     /label=Promoter+5'UTR
                     /note="color: #ff99cc; direction: RIGHT"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /note="This feature has 6 segments:
                        1: 1145 .. 1216 / #993366
                        2: 1618 .. 1891 / #993366
                        3: 1966 .. 2620 / #993366
                        4: 2682 .. 2997 / #993366
                        5: 3218 .. 3349 / #993366
                        6: 3420 .. 4034 / #993366"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    4035..4802
                     /label=3'UTR
                     /note="color: #ff99cc; direction: RIGHT"
     protein_bind    complement(4809..4842)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     primer_bind     4820..4879
                     /label=1139.C1F
                     /note="color: black; sequence: 
                     taatgtatgctatacgaagttatgctagcggatccCTCCGTTGTATGTTGTAGCGTCTG
                     T; added: 2020-04-30"
     primer_bind     complement(4961..5020)
                     /label=1139.C2R
                     /note="color: black; sequence: 
                     ACGAATAACGGCCAGAGAAATTTCTCGActcgagATCAGCTTAGATATCGTAGAGAGTC
                     G; added: 2020-04-30"
     misc_feature    4993..5386
                     /label=Hsp70Bb promote
                     /note="Hsp70 Promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(5347..5406)
                     /label=1098A.C4R
                     /note="color: black; sequence: 
                     TATAGTCCATtttgtttaaaCAGAGTTCTCTTCTTGTATTCAATAATTACTTCTTGGCA
                     G; added: 2019-04-03"
     primer_bind     5370..5429
                     /label=1098A.C5F
                     /note="color: black; sequence: 
                     ACAAGAAGAGAACTCTGtttaaacaaaATGGACTATAAGGACCACGACGGAGACTACAA
                     G; added: 2019-04-03"
     CDS             5400..5465
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 5465"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5472..5492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5517..9617
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9618..9665
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9666..9719
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 9716"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9720..10439
                     /label=GFP
                     /note="GFP"
                     /note="color: #ccffcc; direction: RIGHT"
     5'UTR           10448..11124
                     /label=p10-3'-UTR-Rubin
                     /note="p10-3'-UTR-Rubin"
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(11099..11158)
                     /label=1098A.C6R
                     /note="color: black; sequence: 
                     tgacctacatcgtcgacactagtggatcaggcctCCGGCCGTTAACTCGAATCGCTATC
                     C; added: 2019-04-03"
     misc_binding    11142..11426
                     /label=attB site
                     /note="attB site"
                     /note="color: #8aa6f7"
     primer_bind     complement(11142..11173)
                     /label=NK=252L-2R
                     /note="color: black; sequence: 
                     gcggcttcgagaccgtgacctacatcgtcgac"
     promoter        complement(11451..11469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(11490..11506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    11514..11530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(11538..11568)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 11538 .. 11544 / #ffffff / -10
                        2: 11545 .. 11562 / #ffffff
                        3: 11563 .. 11568 / #ffffff / -35"
     protein_bind    11583..11604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(11892..12480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12651..13511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 12651 .. 13442 / #ccffcc
                        2: 13443 .. 13511 / #ccffcc / signal sequence
                     Cleavage site after base 13442"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13512..13616)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccctccgt
     4861 tgtatgttgt agcgtctgtg aagcgtccta ctcctaagca tgacttgaag cacaacttac
     4921 ctaagccatg acagcctcct gtcgtgtatc actgacagct cgactctcta cgatatctaa
     4981 gctgatctcg agtcgagaaa tttctctggc cgttattcgt tattctctct tttctttttg
     5041 ggtctctccc tctctgcact aatgctctct cactctgtca cacagtaaac ggcatactgc
     5101 tctcgttggt tcgagagagc gcgcctcgaa tgttcgcgaa aagagcgccg gagtataaat
     5161 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     5221 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     5281 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     5341 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgttta aacaaaatgg
     5401 actataagga ccacgacgga gactacaagg atcatgatat tgattacaaa gacgatgacg
     5461 ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca
     5521 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     5581 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     5641 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     5701 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     5761 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     5821 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     5881 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     5941 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     6001 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     6061 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     6121 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     6181 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     6241 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     6301 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     6361 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     6421 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     6481 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     6541 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     6601 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     6661 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     6721 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     6781 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     6841 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     6901 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     6961 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     7021 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     7081 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     7141 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     7201 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     7261 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     7321 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     7381 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     7441 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     7501 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     7561 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     7621 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     7681 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     7741 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     7801 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     7861 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     7921 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     7981 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc
     8041 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     8101 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     8161 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     8221 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     8281 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     8341 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     8401 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     8461 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     8521 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     8581 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     8641 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     8701 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     8761 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     8821 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa
     8881 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     8941 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     9001 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     9061 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     9121 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     9181 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     9241 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     9301 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     9361 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     9421 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     9481 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     9541 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     9601 ctcagctggg aggcgacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga
     9661 aaaaggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     9721 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     9781 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     9841 gcaagctgac cctgaagttc atctgcacca ccagcaagct gcccgtgccc tggcccaccc
     9901 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     9961 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
    10021 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
    10081 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
    10141 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
    10201 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
    10261 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
    10321 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
    10381 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
    10441 taattaacta gaatgaatcg tttttaaaat aacaaatcaa ttgttttata atattcgtac
    10501 gattctttga ttatgtaata aaatgtgatc attaggaaga ttacgaaaaa tataaaaaat
    10561 atgagttctg tgtgtataac aaatgctgta aacgccacaa ttgtgtttgt tgcaaataaa
    10621 cccatgatta tttgattaaa attgttgttt tctttgttca tagacaatag tgtgttttgc
    10681 ctaaacgtgt actgcataaa ctccatgcga gtgtatagcg agctagtggc taacgcttgc
    10741 cccaccaaag tagattcgtc aaaatcctca atttcatcac cctcctccaa gtttaacatt
    10801 tggccgtcgg aattaacttc taaagatgcc acataatcta ataaatgaaa tagagattca
    10861 aacgtggcgt catcgtccgt ttcgaccatt tccgaaaaga actcgggcat aaactctatg
    10921 atttctctgg acgtggtgtt gtcgaaactc tcaaagtacg cagtcaggaa cgtgcgcgac
    10981 atgtcgtcgg gaaactcgcg cggaaacatg ttgttgtaac cgaacgggtc ccatagcgcc
    11041 aaaaccaaat ctgccagcgt caatagaatg agcacgatgc cgacaatgga gctggcttgg
    11101 atagcgattc gagttaacgg ccggaggcct gatccactag tgtcgacgat gtaggtcacg
    11161 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
    11221 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
    11281 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
    11341 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
    11401 tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc cctttagtga
    11461 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    12001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    12061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    12121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    12181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12301 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    12361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    13021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    13081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    13141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    13201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    13261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13621 tttccccgaa aagtgccac
//
