LOCUS       Exported                6751 bp ds-DNA     circular UNA 07-MAY-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     misc_feature    446..469
                     /label=ColonyF_hSyn
     misc_feature    568..626
                     /label=138_F_hSyn_GRAB
     CDS             598..2853
                     /codon_start=1
                     /label=Ado1.0med
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTGPIMGSSVYITVELA
                     IAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAAC
                     HGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSF
                     AIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLL
                     MLGVYLRIFLAARRQLKQMESQPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEA
                     EPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGV
                     QLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
                     TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRGRQNRELHAAKSLAI
                     IVGLFALCWLPLHIINCFTFFCPDCSHAPLWLHYLAIVLSHTNSVVNPFIYAYRIREFR
                     QTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPE
                     RRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS"
     CDS             685..687
                     /codon_start=1
                     /label=1 T deletion
                     /label=1 T deletion
                     /translation="T"
     misc_feature    complement(800..823)
                     /label=Seq1R_hA2AR
     misc_feature    1203..1226
                     /label=Seq1F_hA2AR
     misc_feature    1740..1761
                     /label=EGFP-Cnew
     misc_feature    complement(1867..1888)
                     /label=GFPreverse
     misc_feature    complement(2394..2417)
                     /label=Seq2R_hA2AR
     CDS             2422..2424
                     /codon_start=1
                     /label=M270H
                     /translation="H"
     misc_feature    2730..2753
                     /label=Seq2F_hA2AR
     misc_feature    complement(2830..2888)
                     /label=139_R_AAV_Ado
     unsure          2868..3476
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    complement(2953..2970)
                     /label=pWPTRev
     polyA_signal    3496..3974
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          4014..4154
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4246..4552
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          5071..5928
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6079..6746
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccggt cccatcatgg gctcctcggt gtacatcacg
      721 gtggagctgg ccattgctgt gctggccatc ctgggcaatg tgctggtgtg ctgggccgtg
      781 tggctcaaca gcaacctgca gaacgtcacc aactactttg tggtgtcact ggcggcggcc
      841 gacatcgcag tgggtgtgct cgccatcccc tttgccatca ccatcagcac cgggttctgc
      901 gctgcctgcc acggctgcct cttcattgcc tgcttcgtcc tggtcctcac gcagagctcc
      961 atcttcagtc tcctggccat cgccattgac cgctacattg ccatccgcat cccgctccgg
     1021 tacaatggct tggtgaccgg cacgagggct aagggcatca ttgccatctg ctgggtgctg
     1081 tcgtttgcca tcggcctgac tcccatgcta ggttggaaca actgcggtca gccaaaggag
     1141 ggcaagaacc actcccaggg ctgcggggag ggccaagtgg cctgtctctt tgaggatgtg
     1201 gtccccatga actacatggt gtacttcaac ttctttgcct gtgtgctggt gcccctgctg
     1261 ctcatgctgg gtgtctattt gcggatcttc ctggcggcgc gacgacagct gaagcagatg
     1321 gagagccagc ctcccagccg ccggggtccg gacgccgtcg ccgcgccgcc ggggggcacc
     1381 gagcgcaggc ccaacggtct gggccccgag cgcagcgcgg gcccgggggg cgcagaggcc
     1441 gaaccgctgc ccacccagct caacggcgcc cctggcgagc ccgcgccggc cgggccgcgc
     1501 gacaccgacg cgctggacct ggaggaggga ggaaacgtct atatcaaggc cgacaagcag
     1561 aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg cggcgtgcag
     1621 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1681 aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
     1741 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1801 aagggcggta ccggagggag catggtgaga aagggcgagg agctgttcac cggggtggtg
     1861 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1921 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1981 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2041 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2101 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2161 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2221 gacggcaaca tcctggggca caagctggag tacaacaccg gagcagcagc acgctggcgc
     2281 gggcggcaga accgcgagct ccatgctgcc aagtcactgg ccatcattgt ggggctcttt
     2341 gccctctgct ggctgcccct acacatcatc aactgcttca ctttcttctg ccccgactgc
     2401 agccacgccc ctctctggct ccactacctg gccatcgtcc tctcccacac caattcggtt
     2461 gtgaatccct tcatctacgc ctaccgtatc cgcgagttcc gccagacctt ccgcaagatc
     2521 attcgcagcc acgtcctgag gcagcaagaa cctttcaagg cagctggcac cagtgcccgg
     2581 gtcttggcag ctcatggcag tgacggagag caggtcagcc tccgtctcaa cggccacccg
     2641 ccaggagtgt gggccaacgg cagtgctccc caccctgagc ggaggcccaa tggctacgcc
     2701 ctggggctgg tgagtggagg gagtgcccaa gagtcccagg ggaacacggg cctcccagac
     2761 gtggagctcc ttagccatga gctcaaggga gtgtgcccag agccccctgg cctagatgac
     2821 cccctggccc aggatggagc aggagtgtcc tgagaattcg atatcaagct tatcgataat
     2881 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2941 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3001 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3061 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3121 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3181 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3241 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3301 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3361 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3421 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     3481 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3541 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3601 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3661 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     3721 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3781 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3841 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3901 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3961 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     4021 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4081 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4141 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4201 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4441 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4561 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4681 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4741 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4801 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4861 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4921 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6241 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6721 ctggcctttt gctggccttt tgctcacatg t
//
