LOCUS       Exported                6754 bp ds-DNA     circular UNA 07-MAY-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 7, 2020 from SnapGene Viewer 5.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=Human Synapsin Promoter
                     /label=synapsin
     misc_feature    446..469
                     /label=ColonyF_hSyn
     CDS             598..2856
                     /codon_start=1
                     /label=Ado1.0mut
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGPIMGSSVYITVEL
                     AIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAA
                     CHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLS
                     FAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLAEDVVPMNYMVYFNFFACVLVPLL
                     LMLGVYLRIFLAARRQLKQMESQPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAE
                     AEPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGG
                     VQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMD
                     ELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
                     TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYK
                     TRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRGRQNRELHAAKSLA
                     IIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREF
                     RQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHP
                     ERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS"
     misc_feature    complement(803..826)
                     /label=Seq1R_hA2AR
     CDS             1192..1194
                     /codon_start=1
                     /label=F168a
                     /translation="A"
     misc_feature    1206..1229
                     /label=Seq1F_hA2AR
     misc_feature    1743..1764
                     /label=EGFP-Cnew
     misc_feature    complement(1870..1891)
                     /label=GFPreverse
     misc_feature    complement(2397..2420)
                     /label=Seq2R_hA2AR
     misc_feature    2733..2756
                     /label=Seq2F_hA2AR
     unsure          2871..3479
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    complement(2956..2973)
                     /label=pWPTRev
     polyA_signal    3499..3977
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          4017..4157
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4249..4555
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          5074..5931
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6082..6749
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtcccatca tgggctcctc ggtgtacatc
      721 acggtggagc tggccattgc tgtgctggcc atcctgggca atgtgctggt gtgctgggcc
      781 gtgtggctca acagcaacct gcagaacgtc accaactact ttgtggtgtc actggcggcg
      841 gccgacatcg cagtgggtgt gctcgccatc ccctttgcca tcaccatcag caccgggttc
      901 tgcgctgcct gccacggctg cctcttcatt gcctgcttcg tcctggtcct cacgcagagc
      961 tccatcttca gtctcctggc catcgccatt gaccgctaca ttgccatccg catcccgctc
     1021 cggtacaatg gcttggtgac cggcacgagg gctaagggca tcattgccat ctgctgggtg
     1081 ctgtcgtttg ccatcggcct gactcccatg ctaggttgga acaactgcgg tcagccaaag
     1141 gagggcaaga accactccca gggctgcggg gagggccaag tggcctgtct cgctgaggat
     1201 gtggtcccca tgaactacat ggtgtacttc aacttctttg cctgtgtgct ggtgcccctg
     1261 ctgctcatgc tgggtgtcta tttgcggatc ttcctggcgg cgcgacgaca gctgaagcag
     1321 atggagagcc agcctcccag ccgccggggt ccggacgccg tcgccgcgcc gccggggggc
     1381 accgagcgca ggcccaacgg tctgggcccc gagcgcagcg cgggcccggg gggcgcagag
     1441 gccgaaccgc tgcccaccca gctcaacggc gcccctggcg agcccgcgcc ggccgggccg
     1501 cgcgacaccg acgcgctgga cctggaggag ggaggaaacg tctatatcaa ggccgacaag
     1561 cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga cggcggcgtg
     1621 cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1681 gacaaccact acctgagcgt gcagtccaaa ctttcgaaag accccaacga gaagcgcgat
     1741 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1801 tacaagggcg gtaccggagg gagcatggtg agaaagggcg aggagctgtt caccggggtg
     1861 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1921 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1981 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2041 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2101 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2161 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2221 gaggacggca acatcctggg gcacaagctg gagtacaaca ccggagcagc agcacgctgg
     2281 cgcgggcggc agaaccgcga gctccatgct gccaagtcac tggccatcat tgtggggctc
     2341 tttgccctct gctggctgcc cctacacatc atcaactgct tcactttctt ctgccccgac
     2401 tgcagccacg cccctctctg gctcatgtac ctggccatcg tcctctccca caccaattcg
     2461 gttgtgaatc ccttcatcta cgcctaccgt atccgcgagt tccgccagac cttccgcaag
     2521 atcattcgca gccacgtcct gaggcagcaa gaacctttca aggcagctgg caccagtgcc
     2581 cgggtcttgg cagctcatgg cagtgacgga gagcaggtca gcctccgtct caacggccac
     2641 ccgccaggag tgtgggccaa cggcagtgct ccccaccctg agcggaggcc caatggctac
     2701 gccctggggc tggtgagtgg agggagtgcc caagagtccc aggggaacac gggcctccca
     2761 gacgtggagc tccttagcca tgagctcaag ggagtgtgcc cagagccccc tggcctagat
     2821 gaccccctgg cccaggatgg agcaggagtg tcctgagaat tcgatatcaa gcttatcgat
     2881 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2941 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3001 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3061 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3121 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3181 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3241 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3301 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3361 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3421 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     3481 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     3541 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     3601 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3661 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3721 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3781 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3841 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3901 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3961 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     4021 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4081 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4141 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4201 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4681 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4741 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4801 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4861 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4921 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5281 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5581 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6241 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6721 ttcctggcct tttgctggcc ttttgctcac atgt
//
